Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Biobased itaconzuur en methacrylzuur : Chemische bouwstenen van de toekomst
    Es, D.S. van - \ 2016
    Fluids Processing Benelux (2016)4. - ISSN 1874-7914 - p. 46 - 47.
    biobased economy - chemie op basis van biologische grondstoffen - chemicaliën uit biologische grondstoffen - zuren - biomassa - glucose - biochemie - biobased economy - biobased chemistry - biobased chemicals - acids - biomass - glucose - biochemistry
    Wageningen UR Food & Biobased Research is van plan een flinke stap te zetten in de productie van biobased itaconzuur en methacrylzuur. Deze zuren kunnen bouwstenen zijn voor hoogwaardige materialen, zoals biobased verf en drukinkt. De stoffen worden geproduceerd uit biomassa (glucose) en vormen alternatieven voor fossiele grondstoffen. Voor verdere ontwikkeling wordt samengewerkt met de Amerikaanse agrifoodproducent Archer Daniels Midland, leverancier voor de verfindustrie EOC Belgium en de Nederlandse verfproducent Van Wijhe Verf. Daan van Es, is senioronderzoeker bij Wageningen UR en treedt op als projectleider.
    Energie en eiwitwaardeschatting gras-klaverkuilen
    Schooten, H.A. van; Spek, J.W. - \ 2016
    Wageningen : Wageningen Livestock Research (Wageningen Livestock Research rapport 994) - 33
    grasklaver - eiwitten - energie - voedingswaarde - biochemie - diervoedering - grass-clover swards - proteins - energy - nutritive value - biochemistry - animal feeding
    Plant growth promotion by Pseudomonas fluorescens : mechanisms, genes and regulation
    Cheng, X. - \ 2016
    Wageningen University. Promotor(en): Francine Govers; J.M. Raaijmakers, co-promotor(en): M. van der Voort. - Wageningen : Wageningen University - ISBN 9789462578753 - 192
    soil bacteria - pseudomonas fluorescens - plants - growth stimulators - soil suppressiveness - plant diseases - induced resistance - biochemistry - biosynthesis - plant-microbe interactions - transcriptomics - bodembacteriën - pseudomonas fluorescens - planten - groeistimulatoren - bodemweerbaarheid - plantenziekten - geïnduceerde resistentie - biochemie - biosynthese - plant-microbe interacties - transcriptomica

    Pseudomonas fluorescens is a Gram-negative rod shaped bacterium that has a versatile metabolism and is widely spread in soil and water. P. fluorescens strain SBW25 (Pf.SBW25) is a well-known model strain to study bacterial evolution, plant colonization and biocontrol of plant diseases. It produces the biosurfactant viscosin, a lipopeptide that plays a key role in motility, biofilm formation and activity against zoospores of Phytophthora infestans and other oomycete pathogens. In addition to viscosin, Pf.SBW25 produces other metabolites with activity against plant pathogens. The production of these yet unknown metabolites appeared to be regulated by the GacS/GacA two-component regulatory system (the Gac-system). The second P. fluorescens strain SS101 (Pf.SS101) studied in this thesis is known for its plant growth-promoting activities but the underlying mechanisms and genes are largely unknown. Therefore, in this study, we aimed to identify novel metabolites and biosynthetic genes in Pf.SBW25 and Pf.SS101, and to investigate their role in plant growth promotion and biocontrol. To this end, a multidisciplinary approach involving bioinformatic analysis of the genome sequences of strains Pf.SBW25 and Pf.SS101, microarray-based expression profiling, screening of genomic libraries, bioactivity assays, mass spectrometric image analysis (MALDI-IMS) and GC/MSMS analysis was adopted. In conclusion, we showed that the GacS/GacA two-component system as a global regulator of the expression of genes play important roles in antagonism of Pseudomonas fluorescens toward plant pathogenic microbes as well as in plant growth promotion and ISR. Growth promotion by P. fluorescens is associated with alterations in auxin biosynthesis and transport, steroid biosynthesis, carbohydrate metabolism and sulfur assimilation. Moreover, advanced chemical profiling allowed us to compare the metabolite profiles of free-living P. fluorescens and P. fluorescens living in association with plant roots. A better understanding of yet unknown mechanisms exploited by the various Pseudomonas fluorescens strains will lead to new opportunities for the discovery and application of natural bioactive compounds for both industrial and agricultural purposes.

    Chemie - Van suiker naar mobiel : Kennisclip Bogo-project e-learning
    Baltissen, A.H.M.C. - \ 2016
    Groen Kennisnet
    biopolymeren - suiker - biobased economy - biochemie - chemische reacties - polymelkzuur - melkzuur - bioplastics - tuinbouw - lesmaterialen - biopolymers - sugar - biobased economy - biochemistry - chemical reactions - polylactic acid - lactic acid - bioplastics - horticulture - teaching materials
    Deze kennisclip maakt onderdeel uit van de lesmodule Biobased Economy van het CIV T&U.
    The first wolf found in the Netherlands in 150 years was the victim of a wildlife crime
    Gravendeel, B. ; Groot, G.A. de; Kik, M. ; Beentjes, K. ; Bergman, H. ; Caniglia, R. ; Cremers, H. ; Fabbri, E. ; Groenenberg, D. ; Grone, A. ; Groot Bruinderink, G.W.T.A. ; Font, I. ; Hakhof, J. ; Harms, V. ; Jansman, H.A.H. ; Janssen, R. ; Lammertsma, D.R. ; Laros, I. ; Linnartz, L. ; Marel, D. van der; Mulder, J.L. ; Mije, S. van der; Nieman, A.M. ; Nowak, C. ; Randi, E. ; Rijks, M. ; Speksnijder, A. ; Vonhof, H.B. - \ 2013
    Lutra 56 (2013)2. - ISSN 0024-7634 - p. 93 - 109.
    wolven - fauna - migratie - diergedrag - dna - biochemie - menselijke invloed - centraal-europa - noordoostpolder - wolves - fauna - migration - animal behaviour - dna - biochemistry - human impact - central europe - noordoostpolder
    On July 4th 2013 a dead subadult female wolf-like canid was found by the roadside between Luttelgeest and Marknesse in the Noordoostpolder in the central part of the Netherlands. As the last observations of wild wolves in the Netherlands date back to 1869 the discovery of this animal generated a lot of media attention. European wolf populations have been expanding since the 1950s and the first packs recently established themselves in Germany in geographic proximity of the Dutch border, so natural re-appearance of the species in the Netherlands seemed likely. We investigated the taxonomy of the animal, its geographical origin, and its most recent history. Macroscopic and biochemical analyses of the dead animal convincingly showed that it was a purebred wolf, related to populations from eastern Europe. Bullet impacts and shattered fragments found in the chest and flank, and a discrepancy between the timing of the post mortem and rigor mortis intervals indicated that this wolf was shot prior to illegal transport to the Netherlands. The wolf fed on beaver in either the Carpathian mountains or the Eifel which is too far for the animal to have walked from by itself within the 24 hours needed to digest its last meal. These geographical areas are the only regions where haplotypes and 87Sr/86Sr isotopes retrieved from both the dead wolf and the beaver remains in its stomach co-occur. We therefore conclude that the first Dutch wolf found in the Netherlands in 150 years did not enter the Netherlands by itself but sadly proved to be the victim of wildlife crime. Keywords: Canis lupus, Europe, haplotypes, isotopes, microsatellites, wildlife forensics, wolf.
    Physiology and biochemistry of aromatic hydrocarbon-degrading bacteria that use chlorate and/or nitrate as electron acceptor
    Oosterkamp, M.J. - \ 2013
    Wageningen University. Promotor(en): Fons Stams, co-promotor(en): Caroline Plugge; Peter Schaap. - Wageningen : Wageningen University - ISBN 9789461737779 - 191
    bacteriën - aromatische koolwaterstoffen - fysiologie - biochemie - elektronen - genomen - nitraten - chloraten - microbiologie - bacteria - aromatic hydrocarbons - physiology - biochemistry - electrons - genomes - nitrates - chlorates - microbiology
    The biochemical basis of plant development
    Weijers, D. - \ 2013
    Wageningen : Wageningen University - ISBN 9789461736215 - 23
    fytochemie - plantenontwikkeling - planten - plantenweefsels - celbiologie - moleculaire biologie - biochemie - plantenembryo's - phytochemistry - plant development - plants - plant tissues - cellular biology - molecular biology - biochemistry - plant embryos
    Plants develop highly elaborate structures, ranging from small mosses to large trees. All these structures are made by stem cells and consist of a few basic types of tissue. The field of Biochemistry of Plant Development studies the mechanisms by which regulatory proteins control the formation of stem cells and tissues. The young embryo, developing within the seed, is the simplest model to study these fundamental processes, and to gain understanding of the basis for plant development at cellular, molecular and atomic scale.
    Natuurkwaliteit Drentse vennen opnieuw gemeten : bijna een eeuw ecologische veranderingen
    Dam, H. van; Arts, G.H.P. ; Bijkerk, R. ; Boonstra, H. ; Belgers, J.D.M. ; Mertens, A. - \ 2013
    Amsterdam [etc.] : Herman van Dam Adviseur Water en Natuur [etc.] (Alterra-rapport 2351) - 286
    stilstaand water - plassen - zoetwaterecologie - biochemie - macrofauna - aquatische ecologie - ecologisch herstel - zure regen - verdroging - drenthe - standing water - ponds - freshwater ecology - biochemistry - macrofauna - aquatic ecology - ecological restoration - acid rain - desiccation - drenthe
    In 2010-2011 zijn in 18 Drentse vennen inventarisaties uitgevoerd van beïnvloeding en beheer, waterstandsfluctuaties, waterchemie, vegetatie, kiezelwieren, sieralgen en macrofauna. De resultaten worden vergeleken met die van vergelijkbare inventarisaties in 1990 – 1994 en 2003. In de afgelopen dertig jaar is de kwaliteit van de onderzochte vennen aanzienlijk toegenomen, vooral door de afname van verzurende atmosferische depositie, maar ook door maatregelen tegen eutrofiëring zoals het uitbaggeren van vennen en het bestrijden van meeuwenkolonies. Ook anti-verdrogingsmaatregelen hebben rendement opgeleverd. Gemiddeld is de kwaliteit van de vegetatie, sieralgen en kiezelwieren nu even goed als of zelfs beter dan in de eerste helft van de twintigste eeuw. In de meeste vennen is de kwaliteit van de macrofauna sinds begin jaren negentig niet vooruitgegaan, mogelijk door het slechte koloniserende vermogen van veel macrofaunasoorten, de zuurstofarme waterbodem met toxische zwavelverbindingen en een eventuele ontoereikende omgevingskwaliteit van de vennen. In sommige vennen draagt externe belasting door overnachtende ganzen bij tot eutrofiëring. In vennen waar dat niet het geval is treedt soms interne eutrofiëring op. Door stijging van de pH door afname van verzuring wordt organische stof uit het sediment gemineraliseerd en komen voedingsstoffen vrij in de waterlaag. Vooral in de laatste vijf jaar heeft dat tot achteruitgang van de kwaliteit van algen en macrofauna geleid. Er worden aanbevelingen voor beheer en onderzoek gedaan, zoals het continueren van het huidige beheer, aangevuld met het opnieuw graven van veenputten in dichtgroeiende vennen, het beperken van de invloed van ganzen en grazende runderen en het voorkómen van invasies van exoten. Om de effecten van beleidsmaatregelen en beheer in de toekomst te blijven volgen wordt aanbevolen het onderzoek in alle vennen elke 10-12 jaar te herhalen, aangevuld met frequentere monitoring in een klein aantal vennen.
    Structural and biochemical characterization of 3-hydroxybenzoate 6-hydroxylase
    Montersino, S. - \ 2012
    Wageningen University. Promotor(en): Willem van Berkel, co-promotor(en): A. Mattevi. - [S.l.] : s.n. - ISBN 9789461732781 - 158
    aspecifiek mono-oxygenase - moleculaire structuur - biochemie - unspecific monooxygenase - molecular conformation - biochemistry

    The thesis deals with the characterization of a new flavoprotein hydroxylase 3 hydroxybenzoate 6-hydroxylase (3HB6H) from Rhodococcus jostii RHA1. 3HB6H is able to insert exclusively oxygen in para-position and the enzyme has been chosen to study the structural basis of such regioselectivity. As main result, functional mirror image active sites direct regioselective 3-hydroxybenzoate hydroxylation. Moreover, the nature and role of unprecedented phospholipid binding has been analyzed demonstrating a role in enzyme oligomerization and a possible protective role during catalysis. To conclude, the knowledge acquired improves our insight into the strategies of flavin-dependent regioselective hydroxylation and the results emerged in this thesis provide a foundation for further structural and kinetic studies on 3HB6H and related enzymes.

    Quantification of net exudation for the plant-microbial fuel cell
    Kuijken, R.C.P. ; Snel, J.F.H. ; Bouwmeester, H.J. ; Marcelis, L.F.M. - \ 2011
    wortelexudaten - analyse - microbiële brandstofcellen - biobased economy - biochemie - root exudates - analysis - microbial fuel cells - biobased economy - biochemistry
    The objective of this project is to develop a simple procedure to estimate net root exudation to study genetic and environmental effects on exudation.
    Pectins and pectinases
    Schols, H.A. ; Visser, R.G.F. ; Voragen, A.G.J. - \ 2009
    Wageningen : Wageningen Academic Publishers - ISBN 9789086861088 - 331
    pectinen - biochemie - plantaardige producten - voedselsamenstelling - pectins - biochemistry - plant products - food composition
    Hyperspectral remote sensing of biochemical and biophysical parameters: the derivate red-edge "double-peak feature", a nuisance or an opportunity?
    Cho, M.A. - \ 2007
    Wageningen University. Promotor(en): Andrew Skidmore, co-promotor(en): Sip van Wieren. - [S.l.] : S.n. - ISBN 9789085046226 - 206
    remote sensing - vegetatie - biochemie - biofysica - remote sensing - vegetation - biochemistry - biophysics

    Improved quantification and monitoring of biophysical and biochemical attributes is required to predict the response of ecosystems to climate change and acquire deeper understanding of the carbon cycle. Remote sensing is widely viewed as a time- and cost-efficient way to proceed with large-scale monitoring of vegetation parameters. For over thirty years, use has been made of broadband sensors such as Landsat TM/ETM+. The advent of hyperspectral remote sensing or imaging spectrometry enlarged the number of available bands within the visible, near-infrared (NIR) and shortwave infrared (SWIR). Hyperspectrally detectable variables associated with 1eaf chlorophyll content, phenological state and vegetation stress such as the spectral shift of the red-edge (670-780 nm) slope and its inflection point termed the red-edge position (REP), are not accessible with broadband sensors. State of the art indices and analytical techniques applied for broad-band remote sensing are not always suitable for information extraction from high dimensional hyperspectral data. This study aimed to develop new hyperspectral indices and propose innovative ways for empirically estimating biochemical and biophysical parameters from hyperspectral data.

    The red edge position is estimated using the first derivative of the spectral curve. Existing curve fitting approaches localise the REP while assuming a derivative curve with a single peak. The proposed linear extrapolation method localises the red edge position while explicitly considering two peaks in the derivative curve. The major contribution of this study is that the linear extrapolation method allows optimised estimates of 1eaf chlorophyll or nitrogen content while minimising the confounding effects of background and the structure of leaves and canopy. By minimising these canopy effects, the linear extrapolation may be useful for detecting early physiological stresses associated with changes in 1eaf chlorophyll/nitrogen levels. The linear extrapolation method also shows high potential for discriminating tree and shrub species at both the leafand canopy scales. Lastly, it could be used as a more stable predictor for monitoring green grass biomass in the Majella National park, Italy compared with two-band vegetation indices. The method is simple to implement, but sensitive to spectral noise. Spectral smoothing is recommended when noise is a problem.

    The study also highlights the utility of partial least squares (PLS) regression based on airborne hyperspectral imagery (HyMap) for estimating grass biomass and beech (Fagus sylvatica L.) forest mean diameter-at-breast height (DBH) in the Majella National Park, Italy. PLS regression produced lower prediction errors for grass biomass and beech forest mean DBH compared with univariate regression involving vegetation indices such as NDVI. NDVI may be simple to implement but could be lacking in terms of exploiting the information content inherent in several narrow bands.

    In a nutshell, this study makes a contribution in the domain of information extraction from hyperspectral data for estimating vegetation parameters such as 1eaf chlorophyll/nitrogen concentration, grass biomass and forest structural parameter using empirical models. Other studies are focused on developing physically based methods given the lack of robustness and portability of empirical models for varying environmental conditions. However, empirical models that are less sensitive to environmental conditions such as models based on the linear extrapolation REP could be used to support the development of physically based models, particularly to estimate the values of the model parameters, or to refine the underlying concepts on which the model is constructed. The future of hyperspectral remote sensing could hinge or enhancing the link between empirical and physically based approaches.

    Fractions of ruminant feeds: kinetics of degradation in vitro
    Azarfar, A. - \ 2007
    Wageningen University. Promotor(en): Seerp Tamminga, co-promotor(en): Thomas van der Poel; H. Boer. - [S.l.] : S.n. - ISBN 9789085045571 - 219
    herkauwers - voer - concentraten - ingrediënten - fractionering - kinetica - degradatie - fermentatieproducten - pensfermentatie - in vitro - voedingsfysiologie - biochemie - ruminants - feeds - concentrates - ingredients - fractionation - kinetics - degradation - fermentation products - rumen fermentation - in vitro - nutrition physiology - biochemistry
    A widely adopted procedure to characterise the degradation in the rumen and its dynamics is the in situ incubation technique that assumes the washable fraction of feeds (W) to be equal to the soluble (S) fraction and that both are rapidly and completely degraded which may not be the case. Because W fraction is washed out of nylon bags, their behaviour cannot directly be measured. This thesis, therefore, aimed to characterise the degradative behaviour of some important unprocessed and processed concentrate (barley, maize, milo, peas, lupins and faba beans) ingredients and their fractions, using a methodology that fractionates feed samples into its inherent constituents (non-washable, NWF; insoluble washable, ISWF and soluble washable fraction, SWF) in combination with an in vitro gas production technique. The results show that the size of the W fraction obtained in nylon bag studies differs from that of the soluble fraction. Except in lupins, ISWF of the concentrate ingredients was very rich in starch. SWF was relatively rich in ash, crude protein, soluble sugars, anda residualfraction(chemically not determined)but contained only a negligible quantity of starch. The degradative behaviour of this difference (ISWF), measured with in vitro gas production,isvery similar to that of the non-washable fraction. The nature of the VFA profile resulting from the fermentation of the different fractions differs between fractions and changes with time of fermentation. Except in lupins, expander processing decreases the contribution of the truly soluble fraction (SWF) in concentrate ingredients. In maize, milo, peas and faba beans, gelatinised starch serves as a binding agent reducing the size of ISWF. However, the pelleting after expander processing disturbs the binding effect of gelatinised starch to some extent, and increases the size of ISWF. In all cereal grains, faba beans and lupins, thermo-mechanical processing (expander processing and ensuing pelleting) shows a tendency to increase the fractional rate of substrate degradation and rate of gas production at the early stage of fermentation, thereby shifting the pattern of fermentation towards a more glucogenic fermentation. Moreover, it appears that the expander processing and the ensuing pelleting process provides a certain level of protection to dietary protein as represented by a lowered NH 3 -N production. Our data show that grinding the samples of technological processing changes the particle size distribution in the samples of processed material. As a consequence, the kinetics of gas productionchange. When monitoring the effects of feed processing by using an in vitro gas production technique, no grinding prior to in vitro incubation should be applied.
    Molecular microbial ecology manual
    Kowalchuk, G.A. ; Bruijn, F.J. de; Head, I.M. ; Akkermans, A.D.L. - \ 2004
    Dordrecht : Kluwer - ISBN 9781402021831
    microbiologie - microbiële ecologie - microbiële fysiologie - methodologie - micro-organismen - ecologie - biochemie - microbiology - microbial ecology - microbial physiology - methodology - microorganisms - ecology - biochemistry
    The field of microbial ecology has been revolutionized in the past two decades by the introduction of molecular methods into the toolbox of the microbial ecologist. This molecular arsenal has helped to unveil the enormity of microbial diversity across the breadth of the earth's ecosystems, and has revealed that we are only familiar with a very small minority of the organisms that carry out key microbial functions in diverse habitats. The Molecular Microbial Ecology Manual, Second Edition (MMEM-II) provides a detailed and user-friendly description of the methods that have made this revolution in microbial ecology possible. However, what is perhaps most exciting about MMEM-II is that it contains a large number of new chapters, highlighting the newest trends in microbial ecology research, which seek to provide more quantitative and statistically robust data, and means of coupling microbial identity and function. In addition, the majority of the proven methods described in MMEM's first version have undergone significant revisions to provide the most up-to-date applications available. The state-of-the-art methods described in MMEM-II have not only been provided by experts in the field, but in most cases by the laboratories that actually first developed and applied the methods, thus providing the MMEM-II user with unique first-hand tips and insight.
    Phyllis database uitbreiding met biochemische samenstellingen ten behoeve van de productie van vloeibare en gasvormige brandstoffen
    Rabou, L.P.L.M. ; Lips, S.J.J. - \ 2003
    Petten : ECN (Rapport / ECN ECN-C-03-064) - 24
    biochemie - energiebronnen - biomassa - duurzame energie - biobased economy - biobrandstoffen - databasebeheer - biochemistry - energy sources - biomass - sustainable energy - biobased economy - biofuels - database management
    Eindrapport van het project "Phyllis database uitbreiding met biochemische samenstellingen ten behoeve van de productie van vloeibare en gasvormige brandstoffen"
    Biochemistry and biophysics of tolerance systems
    Buitink, J. ; Hoekstra, F.A. ; Leprince, O. - \ 2002
    In: Desiccation and survival in plants: drying without dying / Black, M., Pritchard, H.W., Wallingford : CABI - p. 293 - 318.
    plantenweefsels - verdroging - biochemie - biofysica - tolerantie - plant tissues - desiccation - biochemistry - biophysics - tolerance
    Pyruvate dehydrogenase : its structure, function and interactions within the pyruvate dehydrogenase multienzyme complex
    Hengeveld, A.F. - \ 2002
    Wageningen University. Promotor(en): C. Veeger; A. de Kok. - S.l. : S.n. - ISBN 9789058086204 - 150
    pyruvaat dehydrogenase (lipoamide) - biochemie - pyruvate dehydrogenase (lipoamide) - biochemistry

    Pyruvate dehydrogenase multi-enzyme complex (PDHC) is member of a family of multienzyme complexes that catalyse the irreversible decarboxylation of various 2-oxoacid substrates to their corresponding acyl-CoA derivatives, NADH and C02. 2-oxoacid dehydrogenase complexes hold key points in primary energy metabolism: PDHC in glycolysis, the oxoglutarate dehydrogenase complex (OGDC) in the citric acid cycle, the branched-chain oxoacid dehydrogenase complex (BCDC) in amino acid catabolism, the glycine dehydrogenase complex (GDHC) in the glycine cleavage system and acetoin dehydrogenase complex (ADHC) in acetoin metabolism. Malfunctioning of a 2-oxo acid dehydrogenase complex in man leads to a broad variety of clinical manifestations, e.g. lactic acidosis (PDHC-deficiency) or maple syrup urine disease (BCDC-deficiency).
    PDHC consists of multiple copies of three enzymes: 2-oxoacid dehydrogenase or Elp-component, dihydrolipoyl acyltransferase (E2p) and dihydrolipoamide dehydrogenase (E3). The E2 component plays a central role in the complex, both catalytically and structurally. It consists of multiple domains (from N- to C-terminus, 1-3 lipoyl domains, an EI/E3-binding domain and a catalytic domain) connected by flexible linkers, allowing a high degree of flexibility of the individual domains. In gram-negative bacteria, the C-terminal catalytic domain forms a cubic core of 24 subunits. Multiple copies of the peripheral components Elp and E3 are non-covalently attached to this core. Three-dimensional structures of the different components and domains of PDHC have been solved by X-ray crystallography or NMR.
    E1 catalyses the rate limiting step in the overall complex reaction; the reductive acylation of the lipoyl groups of E2 utilising thiamin diphosphate (ThDP). The El-component exists either as a homodimer (α 2 or heterotetramer (α 2 β 2 ), depending on the type and source of the complex. There is strikingly little sequence similarity between homodimeric and heterotetranieric E1 or even between homodimeric E1o and homodimeric E1p. An exception to this is the so-called "ThDP-binding motif', that has so far been found in all known ThDP dependent enzymes. Until quite recently EI was by far the least characterised component of the complex. This lack of (structural) information was largely due to the lack of a stable form of the enzyme: when resolved from the complex, E 1 is unstable. To obtain a better insight into the structure and functioning of the El-component of the pyruvate dehydrogenase multienzyme complex from A. vinelandii and its interactions within the complex, a PhD project was started in 1995. The results of this project are presented in this thesis.

    Blood chemistry in southern elephant seal mothers and pups during lactation reveals no effect of handling
    Engelhard, G.H. ; Hall, A.J. ; Brasseur, S.M.J.M. ; Reijnders, P.J.H. - \ 2002
    Comparative Biochemistry and Physiology. A, Molecular and Integrative Physiology 133A (2002)2. - ISSN 1095-6433 - p. 367 - 378.
    fauna - Macquarie - biochemie - ecologie - fauna - zeeolifant - zeezoogdieren - zuidpoolgebied - Grote Oceaan - Macquarie
    Serum clinical chemistry parameters were examined in lactating southern elephant seal Mirounga leonina mothers and their pups from the declining Macquarie Island population. There were significant changes in serum values from 2 to 21 days postpartum in both nursing mothers (increase: inorganic phosphate; decrease: creatinine, potassium, chloride, cholesterol, total protein, albumin, globulin, aspartate aminotransferase, creatine kinase) and suckling pups (increase: inorganic phosphate, globulin, cholesterol; decrease: albumin, alkaline phosphatase, gammaglutamyl transferase; increase followed by decrease: triglycerides, iron). We found no evidence that changes were due to chronic stress effects caused by repeated chemical immobilisations (mothers) or physical restraint (pups): at late lactation, clinical chemistry values were similar for mother-pup pairs of a control group (not handled previously), moderate treatment group (previously handled twice) and high treatment group (previously handled three to four times). We were not able to detect differences in clinical chemistry values between mother-pup pairs distributed over two areas differing in the frequency of human visits. The clinical chemistry values presented here can serve as reference ranges to allow future comparison with other southern elephant seal populations to investigate factors, e.g. food limitation, suspected to be involved in population declines.
    In vitro EROD induction equivalency factors for the 10 PAHs generally monitored in risk assessment studies in The Netherlands
    Bosveld, A.T.C. ; Bie, P.A.F. de; Brink, N.W. van den; Jongepier, H. ; Klomp, A.V. - \ 2002
    Chemosphere 49 (2002)1. - ISSN 0045-6535 - p. 75 - 83.
    biochemie - ecotoxicologie - milieu
    Growth response of a benthic detritivore to organic matter composition in sediments
    Vos, J.H. ; Brink, P.J. van den; Ende, F.P. van den; Ooijevaar, M.A.G. ; Oosthoek, A.J.P. ; Postma, J.E. ; Admiraal, W. - \ 2002
    Journal of the North American Benthological Society 21 (2002)3. - ISSN 0887-3593 - p. 443 - 456.
    biochemie - microbiologie - organische stof - waterbodem
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