Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Toward in vitro fertilization in Brachiaria spp.
    Dusi, D. ; Alves, E.R. ; Willemse, M.T.M. ; Falcao, R. ; Valle, C.B. do; Carneiro, V.T.C. - \ 2010
    Sexual Plant Reproduction 23 (2010)3. - ISSN 0934-0882 - p. 187 - 197.
    pollen-tube - brizantha poaceae - chromosome-number - embryo sac - colchicine - decumbens - guidance - maize - ovule
    Brachiaria are forage grasses widely cultivated in tropical areas. In vitro pollination was applied to accessions of Brachiaria spp. by placing pollen of non-dehiscent anthers on a solid medium near isolated ovaries. Viability and in vitro germination were tested in order to establish good conditions for pollen development. Comparing sexual to apomictic plants, apomictic pollen has more abortion after meiosis during the microspore stage and a lower viability and, of both types, only some plants have sufficient germination in a high sugar concentration. Using in vitro pollination with the sexual plant, the pollen tube penetrates into the nucellus and micropyle, but the embryo sac degenerates and collapses. In the apomictic B. decumbens, in vitro pollination leads to the transfer of the sperm nuclei into the egg cell and the central cell. The results are discussed according to normal fertilization and barriers in sexual and apomictic plants.
    Alteration of the genomic composition of Solanum nigrum (+) potato backcross derivatives by somatic hybridisation: selection of fusion hybrids by DNA measurements and GISH
    Horsman, K. ; Gavrilenko, T. ; Bergervoet, M. ; Huigen, D.J. ; Tjin Wong Joe, A. ; Jacobsen, E. - \ 2001
    Plant Breeding 120 (2001)3. - ISSN 0179-9541 - p. 201 - 207.
    infestans mont debary - lycopersicon-esculentum - chromosome-number - alien chromosomes - rflp analysis - tuberosum - brevidens - tomato - identification - resistance
    Fusion experiments were performed with a first (BC1-6738) and a second (BC2-9017) generation backcross hybrid of 6x Solanum nigrum (+) 2x potato somatic hybrids with potato cultivars. Because no progeny was obtained from the BC2 genotypes, alternative approaches were sought to overcome the sexual crossing barrier. Five potato genotypes, one of which contains the hygromycin resistance gene, were used in the fusion experiments. All vigorous regenerants were used for the estimation of nuclear DNA content using flow cytometry. Plants with a DNA content higher than that of the BC1-6738 or BC2 genotypes were considered potential somatic hybrids. Forty-nine potential somatic hybrids resulted from fusion experiments with BC1-6738, from which 20 grew vigorously in the greenhouse and flowered. After pollination with several 4x potato cultivars. eight genotypes produced seeded berries and five genotypes gave seedless berries. In addition. Il of these 13 somatic hybrids were selected for genomic in situ hybridization (GISH) analysis to determine their genomic composition. Nine had exactly or approximately the expected number of 36 S. nigrum and 60 potato chromosomes. In one genotype. only 22 instead of 36 S. nigrum chromosomes were found acid one potato chromosome was possibly missing. Only five potential somatic hybrids were detected among the 79 regenerants from BC2-9017 (+) 2x potato Fusion experiments that were analysed by flow cytometry. Two of these hybrids were rather vigorous and did flower, but pollinations with potato have not yet yet any berries.
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