Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Zout afvalwater van zeebaarskwekerij geschikt voor teelt van zoutminnende gewassen en een tweede visteeltronde
    Blom, M. ; Heijden, P.G.M. van der - \ 2014
    Aquacultuur 4 (2014)29. - ISSN 1382-2764 - p. 9 - 16.
    aquacultuur - halofyten - combinatie - egypte - aquaculture - halophytes - combination - egypt
    Vis is een belangrijke bron van inkomsten voor Egypte. Niet alleen aan de kust, maar ook in de woestijn worden commerciële viskweekbedrijven opgezet. In Wadi Natroun, het gebied waar in de oudheid het zout vandaan werd gehaald om de overleden farao's te mummificeren, wordt op de boerderij 'Rula for Land Reclamation' door Wadi Fish sinds enige jaren ook vis gekweekt in water uit een zoute bron. In Aquacultuur 2014, nr. 1 is een bedrijfsreportage over Wadi Fish te vinden. Aanvankelijk werd het afvalwater van de kwekerij geloosd in de woestijn. Maar de Egyptische overheid oefent de laatste jaren steeds meer druk uit om zuinig om te gaan met water en met het woestijn-ecosysteem. Daarom is in 2010 een project gestart om te testen of de viskwekerij gecombineerd kan worden met een teelt van halofyten (ofwel zoutminnende gewassen) en of het afvalwater met de zgn. 'biofloc' methode (een waterzuinige viskweekmethode) weer voor visteelt kan worden gebruikt. Het bleek succesvol.
    An Improved Agrobacterium tumefaciens Mediated Transformation of Artemisia annua L. by Using Stem Internodes as Explants
    Tian, N.A. ; Liu, S. ; Huang, J. ; Krol, A.R. van der; Bouwmeester, H.J. ; Liu, Z. - \ 2013
    Czech Journal of Genetics and Plant Breeding 49 (2013)3. - ISSN 1212-1975 - p. 123 - 129.
    regeneration - malaria - plants - combination - drug - dna
    Transformation of Artemisia annua, which produces the sesquiterpenoid endoperoxide artemisinin widely used for the treatment of malaria, has been hampered by the low efficiency of adventitious shoot and root formation on a selective medium containing additional compounds for Agrobacterium decontamination. Here we identified several factors which were all shown to be of importance for optimization of Artemisia annua transformation. Results indicated that stem internodes showed better resistance capacity to Agrobacterium decontaminator than leaves did. Agrobacterium tumefaciens with an optical density (OD) value of 0.2–0.5 plus 100 µmol of acetosyringone per litre of solution gave the best transformation efficiency. Moreover, kanamycin at 30 mg/l in the culture medium was effective in suppressing the growth of non-transformed tissue. Furthermore, transgenic shoots required an early induction of rooting. In addition, dimethyl sulphoxide considerably improved the rooting of shoots. The present work provides rapid and reproducible transformation and regeneration of A. annua
    Persistency of methane mitigation by dietary nitrate supplementation in dairy cows
    Zijderveld, S.M. van; Gerrits, W.J.J. ; Dijkstra, J. ; Newbold, J.R. ; Hulshof, R.B.A. ; Perdok, H.B. - \ 2011
    Journal of Dairy Science 94 (2011)8. - ISSN 0022-0302 - p. 4028 - 4038.
    rumen fermentation - l-cysteine - sheep - manipulation - combination - ruminants - hydrogen - nitrite - cattle
    Feeding nitrate to dairy cows may lower ruminal methane production by competing for reducing equivalents with methanogenesis. Twenty lactating Holstein-Friesian dairy cows (33.2±6.0 kg of milk/d; 104±58 d in milk at the start of the experiment) were fed a total mixed ration (corn silage-based; forage to concentrate ratio 66:34), containing either a dietary urea or a dietary nitrate source [21 g of nitrate/kg of dry matter (DM)] during 4 successive 24-d periods, to assess the methane-mitigating potential of dietary nitrate and its persistency. The study was conducted as paired comparisons in a randomized design with repeated measurements. Cows were blocked by parity, lactation stage, and milk production at the start of the experiment. A 4-wk adaptation period allowed the rumen microbes to adapt to dietary urea and nitrate. Diets were isoenergetic and isonitrogenous. Methane production, energy balance, and diet digestibility were measured in open-circuit indirect calorimetry chambers. Cows were limit-fed during measurements. Nitrate persistently decreased methane production by 16%, whether expressed in grams per day, grams per kilogram of dry matter intake (DMI), or as percentage of gross energy intake, which was sustained for the full experimental period (mean 368 vs. 310±12.5 g/d; 19.4 vs. 16.2±0.47 g/kg of DMI; 5.9 vs.4.9±0.15% of gross energy intake for urea vs. nitrate, respectively). This decrease was smaller than the stoichiometrical methane mitigation potential of nitrate (full potential=28% methane reduction). The decreased energy loss from methane resulted in an improved conversion of dietary energy intake into metabolizable energy (57.3 vs. 58.6±0.70%, urea vs. nitrate, respectively). Despite this, milk energy output or energy retention was not affected by dietary nitrate. Nitrate did not affect milk yield or apparent digestibility of crude fat, neutral detergent fiber, and starch. Milk protein content (3.21 vs. 3.05±0.058%, urea vs. nitrate respectively) but not protein yield was lower for dietary nitrate. Hydrogen production between morning and afternoon milking was measured during the last experimental period. Cows fed nitrate emitted more hydrogen. Cows fed nitrate displayed higher blood methemoglobin levels (0.5 vs. 4.0±1.07% of hemoglobin, urea vs. nitrate respectively) and lower hemoglobin levels (7.1 vs. 6.3±0.11 mmol/L, urea vs. nitrate respectively). Dietary nitrate persistently decreased methane production from lactating dairy cows fed restricted amounts of feed, but the reduction in energy losses did not improve milk production or energy balance
    Bestrijding schimmels met combi van middelen
    Bulle, A.A.E. ; Dijkema, M.H.G.E. ; Hollinger, T.C. ; Haaster, A.J.M. van; Aanholt, J.T.M. van; Korsuize, C.A. - \ 2011
    bestrijdingsmethoden - beschermingsmiddelen - combinatie - schimmelbestrijding - tegen herbiciden resistente onkruiden - preventie - control methods - protectants - combination - fungus control - herbicide resistant weeds - prevention
    Informatieposter over de bestrijding van schimmels met een combinatie van middelen
    CE-LIF-MSn profiling of oligosaccharides in human milk and feces of breast-fed babies
    Albrecht, S.A. ; Schols, H.A. ; Heuvel, E.G.H.M. van den; Voragen, A.G.J. ; Gruppen, H. - \ 2010
    Electrophoresis 31 (2010)7. - ISSN 0173-0835 - p. 1264 - 1273.
    induced fluorescence detection - capillary-electrophoresis - mass-spectrometry - fed infants - sialylated oligosaccharides - liquid-chromatography - preterm - lactose - electrochromatography - combination
    Mixtures of the complex human milk oligosaccharides (HMOs) are difficult to analyze and gastrointestinal bioconversion products of HMOs may complicate analysis even more. Their analysis, therefore, requires the combination of a sensitive and high-resolution separation technique with a mass identification tool. This study introduces for the first time the hyphenation of CE with an electrospray mass spectrometer, capable to perform multiple MS analysis (ESI-MSn) for the separation and characterization of HMOs in breast milk and feces of breast-fed babies. LIF was used for on- and off-line detections. From the overall 47 peaks detected in off-line CE-LIF electropherograms, 21 peaks could be unambiguously and 11 peaks could be tentatively assigned. The detailed structural characterization of a novel lacto-N-neo-tetraose isomer and a novel lacto-N-fucopentaose isomer was established in baby feces and pointed to gastrointestinal hydrolysis of higher-Mw HMOs. CE-LIF-ESI-MSn presents, therefore, a useful tool which contributes to an advanced understanding on the fate of individual HMOs during their gastrointestinal passage.
    Malaria vector control: current and future strategies
    Takken, W. ; Knols, B.G.J. - \ 2009
    Trends in Parasitology 25 (2009)3. - ISSN 1471-4922 - p. 101 - 104.
    insecticide-treated nets - transgenic mosquitos - combination - burden
    The recently announced call for malaria eradication represents a new page in the history of this disease. This has been triggered by remarkable reductions in malaria resulting from combined application of effective drugs and vector control. However, this strategy is threatened by development of insecticide resistance. Efforts to develop alternative tools to complement or even replace insecticide-based vector-control strategies must continue. Here, an overview is presented of the novel vector-control tools expected to contribute to malaria eradication
    Towards Surface Plasmon Resonance biosensing combined with bioaffinity-assisted nano HILIC Liquid Chromatography / Time-of-flight Mass Spectrometry identification of Paralytic Shellfish Poisons
    Marchesini, G.R. ; Hooijerink, H. ; Haasnoot, W. ; Buijs, J. ; Campbell, K. ; Elliott, C.T. ; Nielen, M.W.F. - \ 2009
    TrAC : Trends in Analytical Chemistry 28 (2009)6. - ISSN 0165-9936 - p. 792 - 803.
    biomolecular interaction analysis - prechromatographic oxidation - directed identification - chemical separations - optical biosensor - binding-proteins - toxins - bioassay - assay - combination
    The potential for coupling technologies to deliver new, improved forms of bioanalysis is still in its infancy. We review a number of examples in which coupling has been successful, with special emphasis on combining surface-plasmon-resonance biosensors with mass spectrometry. We give an overview of current progress towards combining biosensor-based bioanalysis with chemical analysis for confirmation of paralytic shellfish poisons that are marine toxins. This comprehensive approach could be an alternative to the official methods currently used (e.g., animal testing and high-performance liquid chromatography with fluorescence detection) and could serve as a model for many more such applications
    Anaerobic biodegradation of estrogens-hard to digest
    Mes, T.Z.D. de; Kujawa, K. ; Zeeman, G. ; Lettinga, G. - \ 2008
    Water Science and Technology 57 (2008)8. - ISSN 0273-1223 - p. 1177 - 1182.
    geactiveerd slib - varkensmest - oestrogenen - afvalwaterbehandeling - anaërobe behandeling - biodegradatie - korrelslib - activated sludge - pig manure - oestrogens - waste water treatment - anaerobic treatment - biodegradation - granular sludge - waste-water treatment - removal - sewage - combination - behavior - sludge - plant
    Although many publications are available on the fate of estrone (E1), 17b-estradiol (E2) and 17a-ethynylestradiol (EE2) during aerobic wastewater treatment, little is published on their fate under strictly anaerobic conditions. Present research investigated the digestibility of E1 and EE2, using digested pig manure, granular UASB sludge, UASB-septic tank sludge and activated sludge as inocula. Besides, actual concentrations were measured in a UASB septic tank treating black water. Under anaerobic conditions E1 is reduced to E2 but the extent of this reduction depends on type of inoculum. No significant loss of the sum of E1 and E2 and of EE2 was observed. Adsorption was responsible for a 32¿35% loss of E1 and E2 from the liquid phase in the UASB septic tank and the effluent still contained considerable concentrations of respectively 4.02 mg/l and 18.79 mg/l for E1 and E2 with a large fraction present in conjugated form. No EE2 was detected in the UASB effluent
    Gecombineerd rooien en sorteren van vruchtbomen kan beter
    PPO Bomen-bollen, - \ 2007
    De Boomkwekerij 2007 (2007)47. - ISSN 0923-2443 - p. 17 - 17.
    vruchtbomen - plantmateriaal - rooien - sorteermachines - combinatie - houding t.o.v. werk - fruit trees - planting stock - grubbing - sorters - combination - attitudes to work
    Er zijn mogelijkheden om de combinatie van het rooien en het sorteren van vruchtbomen te optimaliseren. Recent onderzoek hiernaar door PPO en de Agrotechnology & Food Sciences Group heeft interessante verbeterpunten opgeleverd.
    Resins and additives for powder coatings and alkyd paints, based on renewable resources
    Haveren, J. van; Oostveen, E.A. ; Micciche, F. ; Noordover, B.A.J. ; Koning, C.E. ; Benthem, R.A.T.M. van; Frissen, A.E. ; Weijnen, J.G.J. - \ 2007
    Journal of Coatings Technology and Research 4 (2007)2. - ISSN 1547-0091 - p. 177 - 186.
    combination - acid - linoleate - oxidation
    Due to limited fossil resources and an increased need for environmentally friendly, sustainable technologies, the importance of using renewable feedstocks in the paint and coatings area will increase in the decades to come. This paper highlights some of the perspectives in this area. Alkyd resins for high-solid paints and reactive diluents, completely based on commercially available renewable resources, were prepared and characterized. Alkyd resins based on sucrose and unsaturated fatty acids or oils showed a low intrinsic viscosity, making them suitable to be used in high-solid alkyd paints. Reactive diluents based on similar starting materials showed excellent properties with regard to thinning behavior and effect on drying characteristics. Powder coating polyester resins were synthesized, starting from isosorbide and diacids. Polyester resins with glass transition temperatures up to 70°C were obtained. Incorporation of small amounts of other diols and trifunctional components was found to improve color and coating properties. In order to create completely renewable resin systems, the development of renewable drying agents for alkyds and crosslinkers for powder coatings is in progress.
    Untargeted large-scale plant metabolomics using liquid chromatography coupled to mass spectrometry
    Vos, C.H. de; Moco, S.I.A. ; Lommen, A. ; Keurentjes, J.J.B. ; Bino, R.J. ; Hall, R.D. - \ 2007
    Nature protocols 2 (2007)4. - ISSN 1754-2189 - p. 778 - 791.
    functional genomics - h-1-nmr spectroscopy - metabolites - tomato - identification - arabidopsis - ms - systems - combination - alignment
    Untargeted metabolomics aims to gather information on as many metabolites as possible in biological systems by taking into account all information present in the data sets. Here we describe a detailed protocol for large-scale untargeted metabolomics of plant tissues, based on reversed phase liquid chromatography coupled to high-resolution mass spectrometry (LC-QTOF MS) of aqueous methanol extracts. Dedicated software, MetAlign, is used for automated baseline correction and alignment of all extracted mass peaks across all samples, producing detailed information on the relative abundance of thousands of mass signals representing hundreds of metabolites. Subsequent statistics and bioinformatics tools can be used to provide a detailed view on the differences and similarities between (groups of) samples or to link metabolomics data to other systems biology information, genetic markers and/or specific quality parameters. The complete procedure from metabolite extraction to assembly of a data matrix with aligned mass signal intensities takes about 6 days for 50 samples
    Beta-adrenergic receptor agonists induce the release of granulocyte chemotactic protein-2, oncostatin M, and vascular endothelial growth factor from macrophages
    Verhoeckx, K.C.M. ; Doornbos, R.P. ; Witkamp, R.F. ; Greef, J. de; Rodenburg, R.J.T. - \ 2006
    International Immunopharmacology 6 (2006)1. - ISSN 1567-5769 - p. 1 - 7.
    nitric-oxide production - cyclic-amp - cell-line - modulation - cytokine - combination - transcriptomics - expression - proteomics - pathways
    Vascular endothelial growth factor (VEGF), oncostatin M (OSM), and granulocyte chemotactic protein-2 (GCP-2/CXCL6) are up-regulated in U937 macrophages and peripheral blood macrophages exposed to LPS, beta-adrenergic receptor (ß2-AR) agonists (e.g. zilpaterol, and clenbuterol) and some other agents that induce intracellular cAMP (prostaglandin E2, forskolin, and butyryl cAMP). LPS in combination with ß2-agonists and cAMP elevating agents had an additional effect on the release of VEGF, OSM, and CXCL6. These proteins are up-regulated after 16–24 h of exposure and this is mediated by the ß2-AR, as determined by time course experiments and the use of a specific ß2-AR antagonist (ICI 118551). Beta2-AR agonists are used as bronchodilators in the treatment of asthma, but appear to have no effect on the chronic inflammation of the disease. However, the up-regulation of VEGF, OSM, and CXCL6 may have adverse effects on the inflammatory process of asthma. These mediators are involved in the recruitment of neutrophils, airway remodelling and angiogenesis, known features of chronic inflammatory diseases. We propose that the up-regulation of these proteins could play a role in the adverse effects of prolonged excessive usage of ß2-AR agonists on the airways besides the desensitization of the ß2-AR
    NVO combineert beste van DPS, DPST en PBI tot nieuwe totaalindex. DPS verdwijnt
    Arendonk, J.A.M. van - \ 2006
    Veeteelt 23 (2006)11. - ISSN 0168-7565 - p. 36 - 37.
    melkveehouderij - melkkoeien - dierveredeling - fokwaarde - selectie - koe-indexen - duurzaamheid (sustainability) - combinatie - stamboeken - prestatie-indexen - interviews - uitwendige kenmerken - dairy farming - dairy cows - animal breeding - breeding value - selection - cow indexes - sustainability - combination - herdbooks - performance indexes - interviews - external traits
    De duurzameprestatiesom, DPS, gaat mogelijk nog dit jaar verdwijnen. Ervoor in de plaats komt een nieuwe totaalindex, waarin ook functioneel exterieur een onderdeel zal zijn. Fokkerijprofessor Johan van Arendonk licht de achtergronden toe van de nieuwe totaalindex
    A good life environment for all through conceptual, technological and social innovations
    Lettinga, G. - \ 2006
    Water Science and Technology 54 (2006)2. - ISSN 0273-1223 - p. 1 - 9.
    sewage-treatment system - waste-water treatment - developing-countries - uasb reactor - performance - posttreatment - feasibility - combination
    In conventional environmental protection the parallel development of advanced technical solutions alongside ever more stringent environmental standards increasingly conflicts with the moral and practical imperatives to ensure sustainability and drastically improve the life conditions of the world's poor. Such priorities are far better tackled by technological and social innovations based on relatively simple and highly sustainable concepts: e.g., applying Natural Biological Mineralization Routes (NBMR) for wastewater and waste treatment, implementing Decentralized Sanitation and Resource Recovery and Reuse (DESAR3) where transport of waste(water)s is kept to an optimum level and pollutants valorized, etc. With developing countries now taking a lead in applying these concepts in public sanitation, the more prosperous countries will gradually abandon the expensive, vulnerable and non-sustainable conventional approaches to wastes treatment and environmental protection
    Morphological and molecular characterization of Endophyllum species on perennial asteraceous plants in South Africa
    Wood, A.R. ; Crous, P.W. - \ 2005
    Mycological Research 109 (2005)4. - ISSN 0953-7562 - p. 387 - 400.
    rust fungi - uredinales-pucciniaceae - phylogenetic analysis - osteospermi - combination - infection
    Endophyllum osteospermi is an autoecious, endocyclic rust fungus, which has only been recorded on Chrysanthemoides monilifera ssp. monilifera (Asteraceae, Calendulae), a perennial woody shrub. Both organisms are indigenous to South Africa. Because E. osteospermi is being considered for release in Australia as a biocontrol agent against C. monilifera ssp. monilifera, it was necessary to determine its host range and natural distribution in South Africa. To address this, natural stands of Chrysanthemoides species, as well as other South African asteraceous plants, were monitored for E. osteospermi between 1992 and 2003. A morphological and molecular comparison of specimens referable to Endophyllum was undertaken. Based on these results, E. osteospermi was recorded on C. monilifera sspp. monilifera, pisifera, rotundata, canescens, and subcanescens, C. incana, and an undescribed taxon. E. osteospermi was also recorded on Osteospermum ciliatum, O. polygaloides, and O. potbergense. Furthermore, a closely related but previously undescribed species, E. dimorphothecae sp. nov. is described on Dimorphotheca cuneata. Aecidium elytropappi is transferred to Endophyllum as E. elytropappi comb. nov., being recorded on Elytropappus rhinocerostis and Stoebe plumosa. This study shows that in South Africa E. osteospermi is restricted to a small group of related plant species in the Calenduleae. This rust is therefore considered suitable as a candidate agent for the biocontrol of C. monilifera ssp. monilifera, and pending the results of host specificity testing, would most likely be safe to introduce into Australia.
    Tegelijk composteren en vergisten
    Willers, H.C. ; Veeken, A.H.M. - \ 2005
    Landbouwmechanisatie 56 (2005)3. - ISSN 0023-7795 - p. 14 - 15.
    landbouwwerktuigen - compostering - afvalverwerking - organisch afval - fermentatie - verwerking - combinatie - landbouwkundig onderzoek - farm machinery - composting - waste treatment - organic wastes - fermentation - processing - combination - agricultural research
    Twee onderzoekers van Wageningen UR hebben een concept ontworpen, waarbij composteren en vergisten van een agrarische afvalstroom tegelijk gebeuren. De werking en het resultaat van dit geïntegreerde proces worden hier uitgelegd.
    Significance testing of synergistic/antagonistic, dose level-dependent, or dose ratio-dependent effects in mixture dose-response analysis
    Jonker, M.J. ; Svendsen, C. ; Bedaux, J.J. ; Bongers, M. ; Kammenga, J.E. - \ 2005
    Environmental Toxicology and Chemistry 24 (2005)10. - ISSN 0730-7268 - p. 2701 - 2713.
    vibrio-fischeri - acting chemicals - toxicity - copper - combination - predictability - keratinocytes - diazinon - cadmium - synergy
    In ecotoxicology, the state of the art for effect assessment of chemical mixtures is through multiple dose¿response analysis of single compounds and their combinations. Investigating whether such data deviate from the reference models of concentration addition and/or independent action to identify overall synergism or antagonism is becoming routine. However, recent data show that more complex deviation patterns, such as dose ratio¿dependent deviation and dose level¿dependent deviation, need to be addressed. For concentration addition, methods to detect such deviation patterns exist, but they are stand-alone methods developed separately in literature, and conclusions derived from these analyses are therefore difficult to compare. For independent action, hardly any methods to detect such deviations from this reference model exist. This paper describes how these well-established mixture toxicity principles have been incorporated in a coherent data analysis procedure enabling detection and quantification of dose level¿and dose ratio¿specific synergism or antagonism from both the concentration addition and the independent action models. Significance testing of which deviation pattern describes the data best is carried out through maximum likelihood analysis. This analysis procedure is demonstrated through various data sets, and its applicability and limitations in mixture research are discussed
    Design and development of a DNA array for rapid detection and identification of tomato vascular wilt pathogens
    Lievens, B. ; Brouwer, M. ; Vanachter, A.C.R.C. ; Lévesque, C.A. ; Cammue, B.P.A. ; Thomma, B.P.H.J. - \ 2003
    FEMS Microbiology Letters 223 (2003)1. - ISSN 0378-1097 - p. 113 - 122.
    verticillium-albo-atrum - dot-blot hybridization - f-sp lycopersici - nonpathogenic fusarium-oxysporum - biological-control - genetic-analysis - dahliae - microsclerotia - combination - resistance
    Fusarium wilt, caused by Fusarium oxysporum f. sp. lycopersici, and Verticillium wilt, caused by either Verticillium albo-atrum or Verticillium dahliae, are devastating diseases of tomato (Lycopersicon esculentum) found worldwide. Monitoring is the cornerstone of integrated pest management of any disease. The lack of rapid, accurate, and reliable means by which plant pathogens can be detected and identified is one of the main limitations in integrated disease management. In this paper, we describe the development of a molecular detection system, based on DNA array technology, for rapid and efficient detection of these vascular wilt pathogens. We show the utility of this array for the sensitive detection of these pathogens from complex substrates like soil, plant tissues and irrigation water, and samples that are collected by tomato growers in their greenhouses.
    Practical applications of hydrogenase I from Pyrococcus furiosus for NADPH generation and regeneration
    Mertens, R. ; Greiner, L. ; Ban, E.C.D. van den; Haaker, H.B.C.M. ; Liese, A. - \ 2003
    Journal of Molecular Catalysis. B, Enzymatic 24-25 (2003). - ISSN 1381-1177 - p. 39 - 52.
    catalyzed organic-synthesis - hyperthermophilic archaeon - industrial biocatalysis - alcohol-dehydrogenase - ketone hydrogenation - pyridine-nucleotides - soluble hydrogenase - enzymes - combination - reduction
    The soluble hydrogenase I (H-2:NADP(+) oxidoreductase, EC 1.18.99.1) from the marine hyperthermophilic strain of the archaeon Pyrococcus furiosus was partially purified by anion-exchange chromatography. This P furiosus hydrogenase I preparation (PF H(2)ase I) has been used as biocatalyst in the enzymatic production and regeneration of beta-1,4-nicotinamide adenindinucleotide phosphate, reduced form (NADPH), utilizing cheap molecular hydrogen and forming protons as the only side-product. Any excess of dihydrogen can be removed easily. It could be demonstrated, that this hyperthermophilic hydrogenase exhibits a high stability under reaction conditions. Generation as well as regeneration of NADPH were performed in batch and repetitive batch experiments with recyclisation of the biocatalyst. In two repetitive batch-series 6.2 g l(-1) NADPH could be produced with a total turnover number (ttn: mol produced NADPH/mol consumed enzyme) of 10,000. Utilizing the thermophilic NADPH-dependent alcohol dehydrogenase from Thermoanaerobium spec. (ADH M) coupled to the PF H(2)ase I in situ NADPH-regenerating system, two prochiral model substrates, acetophenone and (2S)-hydroxy-1-phenyl-propanone (HPP), were quantitatively reduced to the corresponding (S)-alcohol and (1R,2S)-diol. An e.e. >99.5% and d.e. >98%, respectively, with total turnover numbers (ttn: mol product/mol consumed cofactor NADP(+)) of 100 and 160 could be reached. (C) 2003 Elsevier B.V. All rights reserved.
    Nisine geholpen met hordentechnologie
    Jong, L.S. de - \ 2001
    Voedingsmiddelentechnologie 34 (2001)8. - ISSN 0042-7934 - p. 49 - 49.
    bacteriocinen - nisine - conserveerkwaliteit - voedselserveermethoden - pathogenen - synergie - combinatie - voedselonderzoek - bacteriocins - nisin - canning quality - food serving methods - pathogens - synergism - combination - food research
    Een combinatie van nisine met carvacrol, thymol of carvon leidde tot een synergistische reductie van het aantal levensvatbare cellen van Listeria monocytogenes en Bacillus cereus. Verslag van een promotieonderzoek
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