|The effect of dehulling and a-glactosidase supplementation on the ileal digestibility of lupin based diets in broiler chickens and adult roosters
Mieczkowska, A. ; Jansman, A.J.M. ; Kwakkel, R.P. ; Smulikowski, S. - \ 2005
Journal of Animal and Feed Sciences 14 (2005). - ISSN 1230-1388 - p. 297 - 304.
polysaccharides - cotyledons - passage - protein - pigs
Two hundred fifty six broiler chickens, 22-days-old, allocated to four groups of four replicate pens with 16 birds each, and six adult ileostomized roosters were used. Four diets containing maize starch and 550 g/kg whole or 458 g/kg dehulled lupin seeds (Lupinus luteus L. cv. Amulet) as the only protein source were offered to birds with or without alpha-galactosidase supplementation. The apparent ileal digestibility of dry matter and nitrogen of the diets was determined. In chickens digesta was collected from the lower part of the ileum immediately after slaughter, while in roosters digesta was collected via a T cannula placed in the terminal ileum during three 12 h periods in a change-over design. Dehulling of lupins significantly increased apparent ileal digestibility of dry matter of the diet by 10% in roosters (P <0.01) and 14% in broilers (P <0.001), while alpha-galactosidase supplementation increased DM digestibility by 3% only in roosters (P <0.05). Dehulling of lupins increased the apparent ileal digestibility of N only in roosters (P <0.01). There was no difference between roosters and broiler chickens with regard to the DM digestibility, but the apparent digestibility of N was lower in roosters, than in broilers. The viscosity of ileal digesta was measured only in chickens. It was generally low and was highest (2.92 mPas.s) in broilers fed the whole lupin diet without alpha-galactosidase. Viscosity was about 37% lower after dehulling the seeds, and decreased about 15% after alpha-galactosidase supplementation.
A xylogalacturonan epitope is specifically associated with plant cell detachment.
Willats, W.G.T. ; McCartney, L. ; Steele-King, C.G. ; Marcus, S.E. ; Mort, A.J. ; Huisman, M.M.H. ; Alebeek, G.J.W.M. van; Schols, H.A. ; Voragen, A.G.J. ; Goff, A. le; Bonnin, E. ; Thibault, J.F. ; Knox, J.P. - \ 2004
Planta 218 (2004)4. - ISSN 0032-0935 - p. 673 - 681.
pectic polysaccharides - spatial regulation - hairy regions - pea hulls - walls - homogalacturonan - tomato - cotyledons - separation - pericarp
A monoclonal antibody (LM8) was generated with specificity for xyloglacturonan (XGA) isolated from pea (Pisum sativum L.) testae. Characterization of the LM8 epitope indicates that it is a region of XGA that is highly substituted with xylose. Immunocytochemical analysis indicates that this epitope is restricted to loosely attached inner parenchyma cells at the inner face of the pea testa and does not occur in other cells of the testa. Elsewhere in the pea seedling, the LM8 epitope was found only in association with root cap cell development at the root apex. Furthermore, the LM8 epitope is specifically associated with root cap cells in a range of angiosperm species. In embryogenic carrot suspension cell cultures the epitope is abundant at the surface of cell walls of loosely attached cells in both induced and non-induced cultures. The LM8 epitope is the first cell wall epitope to be identified that is specifically associated with a plant cell separation process that results in complete cell detachment.