Diaporthe species associated with Vaccinium, with specific reference to Europe
Lombard, L. ; Leeuwen, G.C.M. van; Guarnaccia, V. ; Polizzi, G. ; Rijswick, P.C.J. van; Rosendahl, K.C.H.M. ; Gabler, J. ; Crous, P.W. - \ 2014
Phytopathologia Mediterranea 53 (2014)2. - ISSN 0031-9465 - p. 287 - 299.
phomopsis-vaccinii - maximum-likelihood - south-africa - stem canker - blueberry - grapevines - cranberry - australafricana - inference - diseases
Species of the genus Vaccinium are commercially cultivated in Europe for their berries, which are highly valued for dietary and pharmaceutical properties. Cultivation is severely limited due to a range of fungal diseases, especially those caused by species of Diaporthe. A number of Diaporthe isolates have been collected from Vaccinium growing regions in Europe, and initially identified as D. vaccinii based on host association. Using DNA sequence inference of the combined ß-tubulin, calmodulin, translation elongation factor 1-alpha and the internal transcribed spacer region of the nuclear rDNA, along with morphological characteristics, six species were characterised. Diaporthe eres, D. vaccinii and D. viticola are known species and three novel taxa are described here as D. asheicola, D. baccae and D. sterilis. This study is the first confirmed report of D. vaccinii in Latvia and the Netherlands.
Characterisation of antioxidant and antiproliferative acidic polysaccharides from Chinese wolfberry fruits
He, N.W. ; Yang, X.B. ; Jiao, Y.D. ; Tian, L. ; Zhao, Y. - \ 2012
Food Chemistry 133 (2012)3. - ISSN 0308-8146 - p. 978 - 989.
lycium-barbarum polysaccharides - breast-cancer cells - in-vitro - apoptosis - extraction - arrest - growth - anticancer - prevention - cranberry
Wolfberry fruit polysaccharides (WFPs) were isolated by hot-water extraction and ethanol precipitation. With HPLC analysis, WFPs were for the first time identified as acidic polysaccharides with galacturonic acid being the main component monosaccharide (24.9%), followed by galactose (21.3%), arabinose (18.5%), and glucose (15.9%), accounting for up to 80.6% of the molar percentage. WFPs exhibited a high antioxidant activity and a dose-dependent antiproliferative effect, with IC50 values of 134.9, 70.1 and 138.4 mu g/mL against A549, MCF-7 and LoVo cancer cells after 48 h of incubation as estimated by MTT assay, respectively. Flow cytometry analysis showed that WFPs exerted a stimulatory effect on apoptosis of MCF-7 cells, and induced the cell-cycle arrest at the G0/G1 phase, with the observation of intracellular ROS production and DNA damage. The present study demonstrated that these polysaccharides might have the potential to provide significant natural defence against human cancer.