Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Cell disruption for microalgae biorefineries
    Günerken, E. ; Hondt, E. d'; Eppink, M.H.M. ; Garcia-Gonzalez, L. ; Elst, K. ; Wijffels, R.H. - \ 2015
    Biotechnology Advances 33 (2015)2. - ISSN 0734-9750 - p. 243 - 260.
    microwave-assisted extraction - fluidized-bed adsorption - electric-field treatment - synechocystis pcc 6803 - life-cycle assessment - chlorella-vulgaris - lipid extraction - microbial-cells - saccharomyces-cerevisiae - biodiesel production
    Microalgae are a potential source for various valuable chemicals for commercial applications ranging from nutraceuticals to fuels. Objective in a biorefinery is to utilize biomass ingredients efficiently similarly to petroleum refineries in which oil is fractionated in fuels and a variety of products with higher value. Downstream processes in microalgae biorefineries consist of different steps whereof cell disruption is the most crucial part. To maintain the functionality of algae biochemicals during cell disruption while obtaining high disruption yields is an important challenge. Despite this need, studies on mild disruption of microalgae cells are limited. This review article focuses on the evaluation of conventional and emerging cell disruption technologies, and a comparison thereof with respect to their potential for the future microalgae biorefineries. The discussed techniques are bead milling, high pressure homogenization, high speed homogenization, ultrasonication, microwave treatment, pulsed electric field treatment, non-mechanical cell disruption and some emerging technologies.
    Inactivation of L. plantarum in a PEF microreactor The effect of pulse width and temperature on the inactivation
    Fox, M.B. ; Esveld, D.C. ; Mastwijk, H.C. ; Boom, R.M. - \ 2008
    Innovative Food Science and Emerging Technologies 9 (2008)1. - ISSN 1466-8564 - p. 101 - 108.
    electric-field treatment - kinetics - microorganisms - model
    This article describes the inactivation of Lactobacillus plantarum by pulsed electric fields (PEF) in a microfluidic reactor. The microreactor has the specific advantage that the field intensity can be extremely high with accurate control and measurement of the pulse shape, combined with good temperature controllability. It is demonstrated that the temperature increase due to the ohmic heating of the fluid during treatment is marginal, thereby making this an excellent device for decoupling the temperature and electric field effects of PEF. Flow cytometry measurements showed that the electroporation of cells by PEF is a gradual effect. Reducing the pulsewidth at equal energy inputs did not show a change in inactivation. Higher temperatures showed higher inactivation rates. The effect of the temperature and the electric field strength could be described by a model that combines an Arrhenius equation for temperature dependency with either a Huelsheger or an activation energy based model for electric field dependency.
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