Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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    The cashew allergens : a molecular and serological characterization
    Reitsma, Marit - \ 2017
    Wageningen University. Promotor(en): H.J. Wichers; H.F.J. Savelkoul, co-promotor(en): N.W. de Jong. - Wageningen : Wageningen University - ISBN 9789463430784 - 186
    anacardium occidentale - allergens - serological surveys - protein transport - molecular detection - small intestine - pichia pastoris - in vivo experimentation - sds-page - western blotting - electrophoresis - anacardium occidentale - allergenen - serologische overzichten - eiwittransport - moleculaire detectie - dunne darm - pichia pastoris - in vivo experimenten - sds-page - western blotting - elektroforese

    Cashew nut allergy can be a severe food allergy of which the prevalence appears to be increasing. The aim of this thesis was a comprehensive molecular and serological characterisation of the cashew nut allergens Ana o 1, 2 and 3 for improved diagnosis and characterisation of patient populations.

    Chapter 1 in this thesis provides background information on cashew nuts, allergy, the allergens Ana o 1, 2 and 3, the effect of heat treatments on cashew nut proteins, the digestibility of cashew nut proteins, cross-reactivity between cashew nut proteins and other tree nuts, and the detection of cashew nut in food products. Subsequently, in Chapter 2, a review is presented on the topic of epithelial protein and allergen transport. This review describes multiple pathways of intestinal protein transport, sums up existing experimental data concerning protein and peptide transport, and presents different methods to study this. Interestingly, the pathway of (allergenic) protein transport can differ between sensitized and non-sensitized persons. In sensitized persons, protein transport occurs transcellularly via enterocytes, and paracellularly with the involvement of mast cells, while in non-sensitized persons microfold cells and enterocytes are considered most important.

    In the next three chapters, cashew nut allergens were studied. Cashew nut allergy and cashew nut allergens were chosen because of a high number of undiagnosed cashew nut allergic children reported at the children’s hospital “Kinderhaven”, in Rotterdam, an outpatient clinic that is involved in this study. Chapter 3 describes a protocol for the purification of Ana o 1, 2 and 3 from cashew nuts. Ana o 1 and 3 were purified by protein extraction, salt precipitation and filtering over a 30kDa molecular weight membrane. Ana o 2 was purified by protein extraction followed by gel filtration chromatography. These purified proteins were characterised by SDS-PAGE, western blot, glycoprotein stain, and protein identification. In this chapter also more in-depth analysis was performed on the N- and C-termini of the large and small subunits of Ana o 3. These N- and C-termini of Ana o 3, as well as the SDS-PAGE protein profiles were compared between cashew nuts of different origins in Chapter 4. In this chapter also the effects of different heat treatments on the electrophoretic behaviour of cashew nut allergens from various origins were studied, using both 1D and 2D electrophoresis. In these data no significant differences were detected between the electrophoresis patterns of Ana o 1, 2 or 3 in the various origins of cashew nuts. Some small but significant differences in Ana o 1, 2 and 3 content, however, were detected between the differently heated cashew nuts. No major differences in N- and C-terminal micro-heterogeneity were detected between cashew nuts of different origins.

    Next, in Chapter 5, the cashew nut allergens Ana o 1, 2 and 3 were produced as recombinant proteins using a yeast (P. pastoris) production system. This procedure was used as recombinant allergens often produce higher yields of higher purity compared to native purified allergens. The recombinant proteins were compared to the native cashew nut proteins for their glycosylation pattern, IgE binding capacity, and 2D electrophoresis profile. In Chapter 6, the major findings of this thesis are discussed. An overview of the protein characteristics (e.g. 1D and 2D electrophoresis profile, glycosylation, IgE binding, pepsin-digestibility) was provided, as well as a discussion on the clinical benefits that can be derived from the results obtained in this thesis. Also some additional results are presented, studying the serologic cross-reactivity between cashew nuts and other tree nuts and Anacardiaceae nuts and fruits.

    This thesis provides an in-depth study regarding the protein characteristics of the cashew nut allergens Ana o 1, 2 and 3. Using the allergens that were purified in this thesis project, the serum IgE levels of Ana o 1, 2 and 3 could be measured in cashew nut-allergic children. The allergens were also recombinantly produced to obtain higher quantity of allergens for regular use in diagnostics of cashew nut allergy. The results from this thesis can potentially expand clinical patient characterisation with measurements of IgE levels to purified and recombinantly produced major cashew nut allergens. These results might have applications for other food allergens or patient populations.

    Selective recovery of nickel over iron from a nickel-iron solution using microbial sulfate reduction in a gas-lift bioreactor
    Bijmans, M.F.M. ; Helvoort, P.J. van; Dar, S. ; Dopson, M. ; Lens, P.N.L. ; Buisman, C.J.N. - \ 2009
    Water Research 43 (2009)3. - ISSN 0043-1354 - p. 853 - 861.
    mijnbouw - metallurgie - mijnafval - slib - waterstof - ijzer - nikkel - bioreactoren - elektroforese - sulfaatreductie - slibzuivering - verwijdering - mining - metallurgy - mine tailings - sludges - hydrogen - iron - nickel - bioreactors - electrophoresis - sulfate reduction - sludge treatment - removal - gradient gel-electrophoresis - sulfide precipitation - metal precipitation - heavy-metals - soils - water - ores
    Process streams with high concentrations of metals and sulfate are characteristic for the mining and metallurgical industries. This study aims to selectively recover nickel from a nickel-iron-containing solution at pH 5.0 using a single stage bioreactor that simultaneously combines low pH sulfate reduction and metal-sulfide formation. The results show that nickel was selectively precipitated in the bioreactor at pH 5.0 and the precipitates consisted of >or=83% of the nickel content. The nickel-iron precipitates were partly crystalline and had a metal/sulfur ratio of 1, suggesting these precipitates were NiS and FeS. Experiments focusing on nickel recovery at pH 5.0 and 5.5 reached a recovery of >99.9%, resulting in a nickel effluent concentration
    Image analysis, methanogenic activity measurements, and molecular biological techniques to monitor granular sludge from EGSB reactors fed with oleic acid
    Pereira, M.A. ; Roest, K. de; Stams, A.J.M. ; Akkermans, A.D.L. ; Amaral, A.L. ; Pons, M.N. ; Ferreira, E.C. ; Mota, M. ; Alves, M. - \ 2003
    Water Science and Technology 47 (2003). - ISSN 0273-1223 - p. 181 - 188.
    slib - oleïnezuur - korrels - afbeelden - methaan - elektroforese - microbiële afbraak - afvalwaterbehandeling - sludges - oleic acid - granules - imagery - methane - electrophoresis - microbial degradation - waste water treatment - gradient gel-electrophoresis - diversity - bacteria - biodegradability - identification - toxicity - dna
    Morphological changes in anaerobic granular sludge fed with increasing loads of oleic acid were quantified by image analysis. The combination of this technique with data on-the accumulation of adsorbed long chain fatty acid and with the molecular characterization of microbial community gave insight into the mechanisms of sludge disintegration, flotation and washout
    Morphological changes in anaerobic granular sludge fed with increasing loads of oleic acid were quantified by image analysis. The combination of this technique with data on-the accumulation of adsorbed long chain fatty acid and with the molecular characterization of microbial community gave insight into the mechanisms of sludge disintegration, flotation and washout. It was found that the bacterial domain was more affected than the archaeal domain during this process. However, no acetoclastic activity and only a residual hydrogenotrophic activity were detected in the sludge at the end of the operation.
    Molecular identification and characterisation of bifidobacteria and lactobacilli in the human gastrointestinal tract
    Satokari, R. - \ 2002
    Wageningen University. Promotor(en): W.M. de Vos; E.E. Vaughan; M. Saarela. - S.l. : S.n. - ISBN 9789058085634 - 135
    bifidobacterium - lactobacillus - identificatie - spijsverteringsstelsel - polymerase-kettingreactie - elektroforese - bifidobacterium - lactobacillus - identification - digestive system - polymerase chain reaction - electrophoresis

    Bifidobacteria and lactobacilli are considered to be members of the beneficial microbiota in the human gastrointestinal (GI) tract. The present study describes the development and validation of new molecular methods for the detection and analysis of bifidobacteria and lactobacilli and the application of new techniques to study Bifidobacterium and Lactobacillus populations in the human intestine.

    A method based on genus-specific PCR of 16S rDNA and denaturing gradient gel electrophoresis (DGGE) was developed and validated for profiling Bifidobacterium populations in human faeces. The PCR-DGGE method is a qualitative tool for assessing species/strain composition of complex communities by a single PCR reaction and subsequent resolution of the amplification products by DGGE in a sequence-dependent manner. The approach greatly facilitates the monitoring of faecal samples from large numbers of subjects to reveal bifidobacterial diversity and shifts occurring in it. The identification of DGGE fragments can be done by subsequent cloning and sequencing of the PCR products.

    Genotypic methods were developed and evaluated for the identification and characterisation of Lactobacillus casei -group lactobacilli ( L. casei, L. paracasei, L. rhamnosus , and L. zeae ). L. rhamnosus species-specific PCR was developed and validated. The discriminatory power of the three fingerprinting techniques, pulsed field gel electrophoresis (PFGE), randomly amplified polymorphic DNA (RAPD) and ribotyping, was compared. All three techniques were highly effective in differentiating strains below the species level and they can be placed in the following order with respect to their discriminatory power: PFGE > ribotyping > RAPD.

    Newly developed molecular methods were used to trace ingested probiotic strains L. rhamnosus GG (LGG) and B. lactis Bb12 in the GI-tract. The identity of LGG colonies was verified using a species-specific PCR and Bb12 was detected using the PCR-DGGE method. Both probiotic strains colonised the gut transiently and they were no longer detected in the faeces one week after the end of the administration in most subjects. The synbiotic approach with galactooligosaccharide (GOS) did not prolong the persistence of Bb12. Furthermore, LGG was found to attach in vivo to colonic mucosae and, although the attchment was temporary, to remain for more than a week after discontinuation of LGG administration.

    PCR-DGGE method was used to monitor qualitative changes in adult faecal Bifidobacterium populations in response to B. lactis Bb12 and/or GOS administration. In most subjects two weeks administration of Bb12 and/or GOS did not affect the qualitative composition of indigenous bifidobacterial populations, while Bb12 transiently colonised the gut. Qualitative molecular analysis was used to study the bacterial, bifidobacterial and lactobacilli populations in faeces of breast-fed and formula-fed infants before and after weaning. Genus and group-specific PCRs combined with DGGE and subsequent sequencing of the amplified 16S rDNA fragments revealed no difference in the prevalence or species distribution of Bifidobacterium and Lactobacillus between the two groups of infants. In general, DGGE patterns of 16S rDNA showed equal complexity of bacterial communities in breast-fed and formula-fed infants. Equally intensive changes occurred in the faecal microbiota in infants of both groups due to weaning.

    Elektroforetische identificatie en detectie van plantpathogene organismen
    Rijn, C. van; Kerssies, A. - \ 1996
    Aalsmeer : Proefstation voor Bloemisterij en Glasgroente (Rapport / Proefstation voor Bloemisterij en Glasgroente 29) - 36
    plantenziekteverwekkers - identificatie - elektroforese - phytophthora - gnomonia - cylindrocladium - nederland - fusarium oxysporum f.sp. cyclaminis - fusarium oxysporum f.sp. gladioli - plant pathogens - identification - electrophoresis - phytophthora - gnomonia - cylindrocladium - netherlands - fusarium oxysporum f.sp. cyclaminis - fusarium oxysporum f.sp. gladioli
    Reiniging van dunne meststromen door middel van elektrodialyse
    Gastel, J. van; Vrielink, M. - \ 1996
    Praktijkonderzoek varkenshouderij 10 (1996)1. - ISSN 1382-0346 - p. 20 - 22.
    rundveedrijfmest - schoonmaken - desinfectie - elektrodialyse - elektroforese - filters - filtratie - industrie - vloeibare meststoffen - mest - varkens - elektrochemie - mestoverschotten - mestverwerking - cattle slurry - cleaning - disinfection - electrodialysis - electrophoresis - filters - filtration - industry - liquid manures - manures - pigs - electrochemistry - manure surpluses - manure treatment
    Het Praktijkonderzoek Varkenshouderij onderzocht in samenwerking met Tauw Milieu bv in Deventer de mogelijkheden voor reiniging van dunne meststromen door middel van elektrodialyse. Reiniging tot de lozingsnormen voor het riool lijkt haalbaar. De kosten voor het proces op boerderijschaal zijn echter vooralsnog te hoog.
    Identificatie van anjerrassen met behulp van elektroforese
    Schoot, J. van der - \ 1994
    Wageningen : CPRO-DLO (Intern rapport / CPRO-DLO ) - 15
    rassen (planten) - cultivars - rassen (taxonomisch) - sierplanten - caryophyllaceae - elektroforese - varieties - races - ornamental plants - electrophoresis
    Ontwikkeling van een detectiemethode voor Fusarium oxysporum f.sp. cyclaminis en dianthi gebaseerd op polyacrylamidegel - elektroforese
    Everink, J.T. - \ 1992
    Aalsmeer : Proefstation voor de Bloemisterij (Rapport / Proefstation voor de Bloemisterij 142) - 34
    fusarium oxysporum f.sp. dianthi - detectie - page - elektroforese - fusarium oxysporum f.sp. cyclaminis - fusarium oxysporum f.sp. dianthi - detection - page - electrophoresis - fusarium oxysporum f.sp. cyclaminis
    Optimalisering elektroforesemethode van aardappel ten behoeve van geautomatiseerde identificatie : intern rapport CPRO-DLO
    Schoot, J. van der; Wouters, T.C.A.E. - \ 1992
    Wageningen : CPRO-DLO - 18
    cultivars - descriptoren - elektroforese - identificatie - aardappelen - rassen (taxonomisch) - solanum tuberosum - rassen (planten) - descriptors - electrophoresis - identification - potatoes - races - varieties
    Identificatie van tulperassen met behulp van elektroforese
    Schoot, H. van der - \ 1991
    Wageningen : CPRO-DLO - 14
    cultivars - elektroforese - identificatie - liliaceae - bloembollen - rassen (taxonomisch) - rassen (planten) - tulipa - electrophoresis - identification - ornamental bulbs - races - varieties
    Vergelijkend onderzoek naar de toepasbaarheid van de Nieuwe Nederlandse Niertest voor het aantonen van bacteriegroeiremmende stoffen in nieren van slachtdieren
    Nouws, J.F.M. ; Broex, N.J.G. ; Hartog, J.M.P. den - \ 1986
    Wageningen : RIKILT (Rapport / RIKILT 86.92) - 19
    vleeskeuring - nieren - slachtdieren - residuen - elektroforese - meat inspection - kidneys - meat animals - residues - electrophoresis
    Door vier R.V.V. kringlaboratoria zijn integraal nieren van slachtdieren (noodslachtingen en 1/2%-onderzoek) onderzocht op het voorkomen van bacteriegroeirenm1ende stoffen . Van een aantal als positief beoordeelde nieren (wettelijke test remming >15 mm; nieuwe Nederlandse niertest remming >20 mm), is de bacteriegroeiremmende stof geidentificeerd met behulp van hoogspanningselektroforese. Gedurende het onderzoek is een kwaliteitscontrole-systeem met behulp van standaard controleschijfjes geintroduceerd . De reproduceerbaarheld van het resultaat van de N.N.N.T. werd nagegaan met simultaan geimpregneerde papierschijfjes, welke gedurende 1 week bij -25°C waren bewaard en met de gedurende 2 x 24 uren gekoelde karkasnier.
    Elektroforese en plantenveredelingsonderwijs.
    Suurs, L.C.J.M. - \ 1986
    Landbouwkundig Tijdschrift 98 (1986)4. - ISSN 0927-6955 - p. 31 - 32.
    karakteristieken - elektroforese - zaadcontrole - zaden - characteristics - electrophoresis - seed testing - seeds
    Een standaardmethode is beschreven voor de zuiverheidscontrole van zaden van koolgewassen, uitgewerkt als practicumproef voor grote groepen tweedejaars studenten plantenveredeling. Het principe van de methode, de proefuitvoering en het resultaat zijn uiteengezet
    Onderzoek naar de mogelijkheden om elektroforese en iso-elektrofocusing toe te passen ten behoeve van de veredeling van tuinbouwgewassen
    Dijk, A.J. van - \ 1984
    Wageningen : IVT (Rapport / Instituut voor Veredeling van Tuinbouwgewassen no. 201) - 36
    elektroforese - tuinbouw - plantenveredeling - plantensamenstelling - onderzoek - electrophoresis - horticulture - plant breeding - plant composition - research
    Iso-elektrofocusing is een scheidingsmethode, waarmee van kleine monsters de eiwitten en/of de iso-enzymen van elkaar gescheiden kunnen worden, waarbij het iso-elektrisch punt van deze stoffen bepalend is voor deze scheiding. Bij Lilium, Cucumis en Allium is onderzoek gedaan: om na te gaan of het met gestandaardiseerde methoden mogelijk is voor verschillende gewassen iso-enzympatronen zichtbaar te maken; om verschillen in genotype op te sporen, zodat er in een vroeg stadium selectie kan plaatsvinden; voor het zoeken naar correlaties tussen bepaalde eigenschappen en bepaalde bandenpatronen van eiwitten of iso-enzymen; om verwantschappen tussen soorten binnen een geslacht vast te kunnen stellen
    Melkeiwitten : elektroforese van melkeiwitten
    Anonymous, - \ 1978
    Wageningen : Pudoc (Literatuurlijst / Centrum voor landbouwpublikaties en landbouwdocumentatie no. 4126)
    analyse - dierlijke producten - bibliografieën - controle - zuivelindustrie - elektroforese - melkproducten - melkeiwitten - testen - analysis - animal products - bibliographies - control - dairy industry - electrophoresis - milk products - milk proteins - testing
    Computer simulation of transport patterns of associating macromolecules : with special attention to the electrophoresis of αS1- and β-casein
    Nijhuis, H. - \ 1974
    Landbouwhogeschool Wageningen. Promotor(en): C. Veeger. - Wageningen : Veenman - 56
    elektroforese - fosfoproteïnen - caseïne - computersimulatie - numerieke methoden - electrophoresis - phosphoproteins - casein - computer simulation - numerical methods

    The anomalous boundaries occurring during free electrophoresis or ultracentrifugation of interacting biopolymers are investigated. As is already known from the early studies of LONGSWORTH and MACINNES (1942), such interactions can give rise to abnormal velocities and areas of the migrating peaks in transport patterns.

    In this thesis in particular the complex formation between α S1 - and β-casein two major proteins from milk, was studied. The importance of such complex formation for the cohesion of the natural casein micelles in milk has been stressed repeatedly.

    Complex formation between these proteins was easily recognizable from the different number of moving peaks on both sides of the electrophoretic channel.

    Application of the moving boundary theory (Chapter 2) leads to the conclusion that the constituent mobility of α S1 -casein is fairly high, which indicates the presence of complexes of a high stoichiometric ratio α S1 /β.

    The development of the reaction boundaries during electrophoresis was simulated in the ALGOL-programs presented in Chapter IV. In the computations the self-polymerization of α S1 -casein under the experimental conditions and the simultaneous formation of the various complexes was taken into account. The results of such a simulation was found to be consistent with the conclusions of the moving boundary theory.

    The simulation (Chapter 3) is brought about by dividing the electrophoretic channel into a large number of small boxes of equal length. The development of the reaction boundary then was simulated by means of alternate rounds of transport of material from one box to the next and subsequent re-equilibration. Only the transport due to the velocity is accounted for, which makes the procedure a simplification of GOAD's method for the numerical solution of the conservation-of- mass equation of a system of migrating and interacting macromolecules. By reducing the calculation in this way a diffusion-like error is introduced which is used to advantage to imitate the diffusional flux. It is shown that this procedure is essentially identical to the countercurrent analog developed by BETHUNE and KEGELES to account for the effect of diffusion on the transport pattern of interacting proteins.

    The adjustment of the simulated diffusion coefficients to their actual values is discussed and the results of different calculations compared. It is shown that in agreement with expectation diffusional spreading had only a minor influence on the development of a reaction boundary in prolonged experiments.

    Inactivation of peroxidase, pectinesterase and alkaline phosphatase in polymers as a model for irradiation of dried foodstuffs
    Roozen, J.P. - \ 1972
    Wageningen University. Promotor(en): W. Pilnik. - Wageningen : Pudoc - ISBN 9789022003848 - 11
    effecten - elektrodialyse - elektroforese - enzymen - fermentatie - voedselbestraling - voedselbewaring - voedingsmiddelen - immobilisatie - industrie - plantaardige producten - druk - straling - opslag - groenteproducten - elektrochemie - effects - electrodialysis - electrophoresis - enzymes - fermentation - food irradiation - food preservation - foods - immobilization - industry - plant products - pressure - radiation - storage - vegetable products - electrochemistry

    This thesis consists of a summary only of 6 refereed scientific papers published in Lebensmittel -Wissenschaft und -Technologie 3, 37-40 (1970); 4, 24-26, 93-96, 158-162, 196-200 (1971); 5, 128-131 (1972).

    Aqueous solutions containing an enzyme (peroxidase, alkaline phosphatase, pectinesterase) and a polymer (carboxymethylcellulose, soluble starch, poIyvinylpyrrolidone, sodium pectate) were freeze-dried. The systems thus obtained were incubated over saturated salt solutions at various levels of water activity. They were then irradiated by an electron generator. The storage and radiation sensitivity of the enzymes depended on the polymer used in the model, the enzyme concentration, the humidity and the pH.

    Polyacrylamide Disc electrophorosis of the soluble leaf proteins from Nicotiana tabacum var. "Samsun" and "Samsun NN"
    Loon, L.C. van; Kammen, A. van - \ 1970
    Wageningen : [s.n.] (Mededeling / Laboratorium voor virologie no. 57)
    plantkunde - elektroforese - nicotiana - plantenziekten - afwijkingen, planten - plantenziektekunde - plantenplagen - gewasbescherming - eiwitten - tabak - botany - electrophoresis - plant diseases - plant disorders - plant pathology - plant pests - plant protection - proteins - tobacco
    Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotiana tabacum var. "Samsun" and "Samsun NN", 1
    Loon, L.C. van; Kammen, A. van - \ 1968
    Wageningen : [s.n.] (Mededeling / Laboratorium voor virologie no. 52) - 9
    plantkunde - elektroforese - nicotiana - plantenziekten - afwijkingen, planten - plantenziektekunde - plantenplagen - gewasbescherming - eiwitten - tabak - botany - electrophoresis - plant diseases - plant disorders - plant pathology - plant pests - plant protection - proteins - tobacco
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