Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Haemolytic Complement Activity and Humoral Immune Responses to Sheep Red Blood Cells in Indigenous Chickens and in Eight German Dahlem Red Chicken Lines with Different Combinations of Major Genes (dwarf, naked neck and frizzled) of Tropical Interest
    Baelmans, R. ; Parmentier, H.K. ; Nieuwland, M.G.B. ; Dorny, P. ; Demey, F. ; Berkvens, D. - \ 2005
    Tropical Animal Health and Production 37 (2005)3. - ISSN 0049-4747 - p. 173 - 186.
    low antibody-production - white leghorn chickens - histocompatibility complex - divergent selection - laying hens - immunocompetence - erythrocytes - multitrait - antigens - serum
    A total of 376 chickens from different ecotypes were immunized with the non-pathogenic multideterminant antigen sheep red blood cells (SRBC). The ecotypes included indigenous chickens from various locations in Tanzania (n = 102), India (n = 86) and Bolivia (n = 89). In addition, eight German Dahlem Red (GDR) chicken lines with different major genes (dwarf, naked neck and frizzled) of tropical interest were also immunized with SRBC. Immune competence of the breeds was assessed by measuring complement haemolytic activity, both from the classical calcium-dependent complement pathway (CPW) and alternative calcium-independent complement pathway (APW), alongside IgTotal, IgG and IgM antibody responses to SRBC at 7 days post immunization. Large variations in complement activity and antibody responses to SRBC were observed within and between the indigenous breeds. Many indigenous chickens, especially from Bolivia, showed decreased complement activity (APW) following immunization with SRBC. Breeds from India showed the highest CPW activity and humoral (especially IgM) responses to SRBC, suggesting high immune competence. In contrast, Bolivian chickens were characterized by low CPW activity, low APW activity and low antibody levels to SRBC suggesting an overall low immune competence. In the GDR chickens, characterized by high CPW activity and high IgG antibody responses to SRBC, the major genes for naked neck, frizzling and dwarfism had no significant effect on the antibody responses and complement activity to SRBC.
    Characterisation of fish leucocytes : an immunocytochemical and functional study in carp (Cyprinus carpio L.)
    Koumans - van Diepen, J.C.E. - \ 1993
    Agricultural University. Promotor(en): W.B. van Muiswinkel, co-promotor(en): J.H.W.M. Rombout. - S.l. : Koumans-van Diepen - ISBN 9789054851110 - 167
    cyprinidae - karper - bloedserum - fibrine - bloedplaatjes - bloed - erytrocyten - leukocyten - bloedplasma - reticulo-endotheliaal systeem - antilichamen - immunoglobulinen - immunocytochemie - cyprinidae - carp - blood serum - fibrin - platelets - blood - erythrocytes - leukocytes - blood plasma - reticuloendothelial system - antibodies - immunoglobulins - immunocytochemistry

    A panel of monoclonal antibodies (MAbs) against carp serum immunoglobulin (Ig), WCIs or carp thymocytes (T), WCTs were used for the characterisation of carp leucocytes. Unfortunately, all WCTs and some WCIs react with common carbohydrate determinants present on all leucocytes and Ig. Most WCIs react specific with protein determinants at the heavy chain of Ig. Consequently, B lymphocyte (sub) populations, plasma cells and Ig-binding cells could be studied. Ig molecules are found in clusters at the cell membrane of B cells and plasma cells, and in contrast to mammalian plasma cells, most carp plasma cells still have Ig at their surface membrane. Mainly the dull surface Ig-positive (sIg +) cells were stimulated by the mammalian B cell mitogen LPS and not by PHA (T cell mitogen) in vitro , whereas the sIg-negative (sIg -) cells were stimulated by PHA and not by LPS. The percentages of B cells and plasma cells showed an increase during ontogeny and reached a plateau at about 3 months and 8 months of age respectively. It is suggested that full development of the carp (humoral) immune system needs at least 8 months (at 21-22 °C). Three different subpopulations of B cells and plasma cells and at least two Ig isotypes can be distinguished based upon their reactivity with WCI 4 arid WCI 12. The distribution of the three B cell subpopulations appeared to be organ and age dependent which indicates functional differences between the Ig isotypes. Fc-like receptors were mainly demonstrated on gut macrophages while pronephros macrophages and neutrophilic granulocytes did not show Ig binding. Consequently, other forms of antigen opsonisation (e.g. complement) may play a role in phagocytosis by these non Ig-binding cells. Several procedures were tested for obtaining MAbs specific for Ig -lymphoid cells. It is concluded that the presence of immunodominant carbohydrate determinants is the major problem for obtaining specific MAbs. Tolerisation of mice against these determinants or the use of isolated membrane lysates from (sIg -) PBL appeared promising but till now only specific thrombocyte markers have been obtained. The use of more purified antigen is recommended in further attempts. The data presented in this thesis can be used for fundamental studies on cell interactions in the immune response, but also for more applied investigations on fish health control.

    Influence of wet vs. dry by-product ingredients and addition of branched-chain volatile fatty acids and valerate to dairy diets. 3. Kinetics of rumen ingesta turnover, whole tract digestibility, and some plasma hormone concentrations.
    Robinson, P.H. ; Tamminga, S. ; Vuuren, A.M. van - \ 1987
    Netherlands Journal of Agricultural Science 35 (1987)4. - ISSN 0028-2928 - p. 459 - 471.
    blood - blood plasma - blood serum - carboxylic acids - dairy cattle - dairy farming - endocrinology - erythrocytes - fatty acids - fibrin - hormones - leukocytes - platelets - rumen - rumen digestion - rumination
    Genetical and some environmental influences affecting the level of leucocyte counts in the milk of cows
    Afifi, Y.A. - \ 1967
    Wageningen University. Promotor(en): T. Stegenga. - Wageningen : Veenman - 81
    rundvee - rauwe melk - diergeneeskunde - melkklieren - melksecretie - lactatie - dierlijke producten - vervalsing - besmetting - verouderen - gebreken - achteruitgang (deterioration) - bloedserum - fibrine - bloedplaatjes - bloed - erytrocyten - leukocyten - bloedplasma - mastitis - cattle - raw milk - veterinary science - mammary glands - milk secretion - lactation - animal products - adulteration - contamination - aging - defects - deterioration - blood serum - fibrin - platelets - blood - erythrocytes - leukocytes - blood plasma - mastitis
    The progeny groups of different sires varied widely in white-cell count in milk, even after exclusion of all cows which had suffered from mastitis. The sire had a demonstrable effect on white-cell count in milk, especially during the second half of lactation. Heritability estimates of white-cell count in milk showed that values for the fourth lactation were higher than those for heifers. But at the end of lactation heritability values for 4th lactation cows and heifers were nearly equal (about 0.40). The daughter groups with high average white-cell counts mostly showed frequent mastitis. There was a high phenotypic and genetic correlation between clinical mastitis and white-cell count. Within seasons for cows which, so far known, had never mastitis, very high and very low producers had higher white-cell counts than other cows. White-cell counts increased remarkably with advancing lactation. A relation between white-cell count and ease of milking could not be demonstrated.

    Increasing milking vacuum over 40 cm mercury pressure, especially at the end of lactation, or increasing pulsation to over 50 per min. tended to increase white-cells. Milking routine (man/machine ratio) affected white-cell count in the end of lactation. More cows per milker increased the number of white-cells.

    Verspreiding van bloedgroepen in het Nederlandse zwartbonte rundvee : een onderzoek naar de frequenties van bloedgroepen en naar enige factoren, die de frequenties beinvloeden
    Kraaij, G.J. - \ 1967
    Wageningen University. Promotor(en): T. Stegenga. - Wageningen : Veenman - 127
    bloedserum - fibrine - bloedplaatjes - bloed - erytrocyten - leukocyten - bloedplasma - rundvee - melkveerassen - nederland - genetica - heritability - genetische variatie - blood serum - fibrin - platelets - blood - erythrocytes - leukocytes - blood plasma - cattle - dairy breeds - netherlands - genetics - heritability - genetic variation
    Blood groups are genetically determined components of the red blood cells. In cattle there were 13 loci known to determine blood groups and some of these loci had large series of alleles. There were also 14 other loci known to determine proteins and enzymes in blood and milk of cattle.

    The author examined how the distribution of blood groups in the Dutch Friesian population had been influenced by the restrictions of breeders. He found that parent cattle were paired independently of blood group. In offspring there was no selection for a certain phenotype until the end of the first year.

    There were clear differences in the frequency of some genes between adult bulls and cows. The gene for blood group A was less frequent in bulls and that for blood group F was less frequent in cows. This occurred in some foreign breeds of cattle.

    The distribution of blood groups over the population was not even. There were differences in gene frequency between breeding areas and in the breeding areas there were large differences between artificial insemination stations. Differences within farms and within breeding pedigrees were even greater. These differences could be ascribed largely to the use of one or only a few sires.

    Het kopergehalte van lever en bloedserum bij het Fries-Hollandse rund
    Grift, J. van der - \ 1955
    's-Gravenhage : Staatsdrukkerij (Verslagen van landbouwkundige onderzoekingen 61.10) - 61
    melkveerassen - rundvee - bloedserum - fibrine - bloedplaatjes - bloed - erytrocyten - leukocyten - bloedplasma - dairy breeds - cattle - blood serum - fibrin - platelets - blood - erythrocytes - leukocytes - blood plasma
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