Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Functional aspects of baculovirus DNA photolyases
    Xu, F. - \ 2010
    Wageningen University. Promotor(en): Just Vlak, co-promotor(en): Monique van Oers. - [S.l. : S.n. - ISBN 9789085857730 - 112
    baculoviridae - organismen ingezet bij biologische bestrijding - insectenplagen - fotolyse - chrysodeixis chalcites - kernpolyedervirussen - lyasen - genen - genexpressie - fylogenetica - lokalisatie - ultraviolette straling - eiwitexpressieanalyse - gevoeligheid - baculoviridae - biological control agents - insect pests - photolysis - chrysodeixis chalcites - nuclear polyhedrosis viruses - lyases - genes - gene expression - phylogenetics - localization - ultraviolet radiation - proteomics - sensitivity
    Keywords: baculovirus, ChchNPV, CPD photolyase, phylogeny, UV resistance, DNA binding, localization, proteomics

    Baculoviruses are insect viruses that are applied as biological control agents due to adequate virulence, host specificity and safety for the environment. Solar light negatively affects field performance of baculoviruses by reducing their infectivity, most likely as a consequence of the formation of cyclobutane pyrimidine dimers (CPDs) in the viral DNA upon ultraviolet (UV) irradiation. CPDs can be repaired by CPD photolyases when exposed to blue light photons, a process called photoreactivation. From previous work it was known that the Cc-phr2 gene of the baculovirus Chrysodeixis chalcites nucleopolyhedrovirus (ChchNPV) encodes a biochemically active photolyase. The research in this thesis focuses on (i) the degree of conservation of CPD photolyase (phr) genes in a subgroup of baculoviruses, (ii) the localization of baculovirus photolyase proteins in insect cells and occlusion derived virus (ODV), and (iii) the in vivo effect of phr genes on the UV sensitivity of baculoviruses. Homologues of the Cc-phr genes were found in all studied group II NPVs in the genus Alphabaculovirus that infect insects in the subfamily Plusiinae insects. Phylogenetic analysis suggested that these phr-like genes have a common ancestor. Intracellular localization of the two ChchNPV encoded PHR proteins in insect cells was studied using enhanced GFP fusion. Both PHR1 and PHR2 localized in the nucleus and associated with chromosomes, spindle, aster and midbody structures during host cell mitosis. Moreover, Cc-PHR2 co-localized with virogenic stroma, when PHR2-EGFP-transfected cells were infected with Autographa californica (Ac) MNPV. Neither of the two Cc-PHR proteins was identified by LC/MS-MS in the ODVs of ChchNPV. To evaluate the potential of the Cc-PHR2 protein to reduce the UV sensitivity of a baculovirus, the Cc-phr2 gene was incorporated in the genome of Helicoverpa armigera (Hear) NPV, which does not have a UV damage repair system. This resulted in a decreased sensitivity to UV-light compared to wild type HearNPV. A cell line was established from embryos of the insect C. chalcites. This cell line was shown to be permissive for both ChchNPV and the related Trichoplusia ni NPV (TnSNPV). This novel cell line will be a useful tool for making ChchNPV phr mutant viruses to study the impact of DNA repair mediated by photolyases on baculovirus ecology. The collected data support the hypothesis that the Cc- phr2 gene provides a baculovirus with an ecological benefit by increasing the resistance to UV.

    Ivoren toren op het vlakke land
    Rekittke, J. - \ 2007
    Topos : periodiek over landschapsarchitectuur, ruimtelijke planning en sociaal-ruimtelijke analyse 17 (2007)1. - ISSN 1572-302X - p. 34 - 35.
    academische maatstaven - studieresultaat - universitaire onderwijsprogramma's - universiteiten - persoonlijke ontwikkeling - persoonlijkheid - oriëntatie - karakteristieken - lokalisatie - landschapsarchitectuur - fysiografische elementen - reorganisatie - academic standards - academic achievement - college programs - universities - personal development - personality - orientation - characteristics - localization - landscape architecture - physiographic features - reorganization
    Twee columns in het thema: ‘Bakens van/en herkenning’ Krostof van Assche over het nut van de variatie in bakens en ‘Ivoren toren op het vlakke land’ van Jörg Rekittke over academische waarden en opbouwen van een moderne universiteit
    Robotic harvesting of vegetable fruit
    Zedde, H.J. van de; Pekkeriet, E.J. - \ 2007
    robots - plukken (picking) - komkommers - kassen - lokalisatie - beeldverwerking - videocamera's - glastuinbouw - patroonherkenning - beeldvormende spectroscopie - 3d visualisatie - robots - picking - cucumbers - greenhouses - localization - image processing - video cameras - greenhouse horticulture - pattern recognition - imaging spectroscopy - 3d visualization
    Since labour costs are high and still increasing, automated harvesting of fruits as cucumber, paprika and tomato will be important in the near future. Supported by the Dutch Ministry of Agriculture, Food and Fishery a project to develop a system for robot harvesting of cucumbers in greenhouses started in 1996. The robot harvester is mounted on an autonomous vehicle that serves as a mobile platform. The robot can move through the greenhouse using the heating pipes (figure 1). Three important topics of this research are vision systems, fruit detection and 3d localization
    Ruimtelijke gadgets in de agri-sector
    Jong, S.M. de; Bregt, A.K. - \ 2001
    Agro Informatica 14 (2001)1. - ISSN 0925-4455 - p. 11 - 14.
    technische vooruitgang - landbouw - lokalisatie - remote sensing - satellieten - geografische informatiesystemen - spectrometers - precisielandbouw - technical progress - agriculture - localization - remote sensing - satellites - geographical information systems - spectrometers - precision agriculture
    Gekeken wordt naar een aantal technologische ontwikkelingen van de laatste jaren, zoals Global Positioning System, hoge resolutie satellietbeelden, beeldvormende spectrometers en low budget remote sensing, en hoe deze ontwikkelingen een weg vinden in de agri-sector, bijvoorbeeld binnen de precisielandbouw
    Meten en beoordelen variatie in grasopbrengst binnen graslandpercelen
    Lokhorst, C. ; Radersma, H. ; Kasper, G. ; Werkhoven, C. - \ 1997
    Agro Informatica 10 (1997)5. - ISSN 0925-4455 - p. 5 - 7.
    droge stof - graslanden - geografische informatiesystemen - positie - lokalisatie - optische instrumenten - meting - geneeskunde - schatting - gewassen - oogstvoorspelling - geodesie - dry matter - grasslands - geographical information systems - position - localization - optical instruments - measurement - medicine - estimation - crops - yield forecasting - geodesy
    De auteurs laten zien dat het mogelijk is opbrengstkaarten van grasland te produceren en geven inzicht in de informatieaspecten die daarbij een rol spelen
    Geografische informatie voor plaats specifieke werkzaamheden.
    Goense, D. ; Hofstee, J.W. ; Bergeijk, J. van - \ 1995
    Agro Informatica 8 (1995)3. - ISSN 0925-4455 - p. 28 - 32.
    geografische informatiesystemen - lokalisatie - positie - precisielandbouw - geodesie - geographical information systems - localization - position - precision agriculture - geodesy
    Presentatie van een gestructureerd informatiemodel als basis voor gegevensverwerking met betrekking tot plaatsspecifieke veldwerkzaamheden, waarbij ook het gebruik van coordinaatsystemen aan de orde komt
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