Records 1 - 20 / 706
On the genetic mechanisms of nutrient-dependent lifespan and reproduction
Zandveld, Jelle - \ 2017
Wageningen University. Promotor(en): B.J. Zwaan, co-promotor(en): A.J.M. Debets. - Wageningen : Wageningen University - ISBN 9789463436861 - 209
genetics - lifespan - reproduction - nutrients - drosophila melanogaster - fungi - diet - evolution - genetica - levensduur - voortplanting - voedingsstoffen - drosophila melanogaster - schimmels - dieet - evolutie
Dietary restriction (DR), a moderate reduction in nutrient intake, improves health or extends lifespan across many species. Moreover, recent insights have shown that also the effects of specific nutrients are of importance for the beneficial effects of DR rather than intake alone. However, we still lack much insight through what mechanisms the lifespan increase through diet changes is exactly mediated.
To further increase our understanding of the genetic mechanisms of nutrient-dependent lifespan, in Chapter 2, 3, 4, and 5 I employed different methods of genetic interventions (i.e. a genetic knockout, natural genetic variation and experimental evolution) using the model species Drosophila melanogaster and Podospora anserina. To test whether the genetic interventions affected the diet response, a broad range of diets was applied, thereby taking the recent insights of nutritional geometry into account. Furthermore, the response of the fly’s whole-genome transcription to different dietary treatments were assessed in Chapter 6 and 7 to identify and potentially disentangle genetic mechanisms for lifespan from those for reproduction.
Chapter 2 addressed the effects of a triple knockout in the insulin-IGF signalling (IIS) pathway, namely for three genes encoding insulin-like peptides in Drosophila (dilp2-3,5). The mutant showed a strong elevation of lifespan that was irrespective of food type, but also a strong reduction of the female fly fecundity. In addition, this assay also revealed that the same knockout can yield different interpretations for its function in the fly’s diet response, which was strongly dependent per diet dimension under consideration (i.e. varying yeast, sugar, or its ratio in the diet). This observation set the stage for other experimental chapters in this thesis, where a broad range of diets was applied to depict the exact genotypic effects that are involved in the lifespan response to diet. For example, in Chapter 2, interactive effects were observed between dilp2-3,5 knockout and the lifespan response to dietary sugar, but however, not for the yeast component of the diet.
In Chapter 3, for the same experimental diets, gene expression responses in dilp2-3,5 knockout flies were measured to describe the general dynamics on the pathway level. Interestingly, expression of the remaining fly head-expressed dilp, dilp6, was elevated on higher yeast levels upon dilp2-3,5 knockout. Therefore, compensatory mechanisms within IIS might still partly mediate the lifespan response to yeast.
In Chapter 4 the natural genetic variation for the response to DR was explored in wild-derived strains of the fungus Podospora anserina. By applying a broad range of glucose concentrations in a synthetic medium, we constructed reaction norms for 62 natural occurring strains and showed considerable natural variation in the shape of the reaction norms, including the glucose concentration at which lifespan increased and how steeply the fungus’ lifespan responds to diet (the slope S). Furthermore, I identified a significant correlation between a strain’s general lifespan and both parameters, suggesting that the lifespan response to diet partly acts through a mechanism involved in the fungus’ lifespan determination under high nutrient, growth and reproduction permissive, conditions. On moderate glucose restriction levels we showed that a reduced reproduction was not always associated with lifespan extension, which indicates that decoupling of these traits (that often trade-off) can be achieved.
An evolutionary perspective on diet response and the connection between reproduction and lifespan, two often interconnected traits in lifespan research, was provided in Chapter 5. Here, experimental evolution (EE) was performed in Drosophila melanogaster to test whether improved reproductive capacity (i.e. local adaptation) to three nutritionally distinct diets directly affected the lifespan response. Adaptation to the distinct nutritional conditions, had no consistent effect on the lifespan response to diet. Other life-history traits that I assessed could more consistently be associated with the evolutionary nutritional treatments, which together suggested that the adaptive genetic mechanisms increasing the fly’s reproduction were not necessarily interconnected singly with a change of lifespan, but rather with a change in the whole life-history strategy.
By exploring the fly’s whole-genome transcription response in a continuously changing environment, Chapter 6 continued on the evolutionary relevance of lifespan responses to diet. This type of fluctuations may better reflect the fly’s natural ecological setting than the continuous diets typically applied in whole-genome transcription laboratory studies. This revealed that flies were able to respond quickly to diet fluctuations throughout lifespan by drastically changing their transcription pattern and, moreover, my results indicated that a large part of the whole-genome transcription response could be attributed to the female fly’s reproduction. Because I measured the response of multiple life-history traits to the fluctuating diet changes, I was able to decouple groups of genes associated with lifespan from those associated with reproduction. This is an important step in the direction of unravelling the genetic architecture that specifically mediates the lifespan response to diet, which can be especially useful in whole-genome transcription studies.
In Chapter 7, the consistencies between studies for their whole-genome transcription responses upon DR were investigated. This revealed large transcriptomic variations on different regulatory levels, i.e. the level of whole-genome transcription, most significant genes, and also gene ontology. To test whether the observed inconsistent whole-genome transcription responses were primarily a reflection of the fly’s reproduction, such as observed in Chapter 6, a new cohort of flies was subjected to different regimes that resulted in very different age-dependent reproduction patterns. By assessing whole-genome transcription in this cohort at two time points, the gene expression changes reflected the age-dependent reproduction patterns observed, rather than the lifespan phenotypes. Similar to Chapter 6, this again highlighted the importance of measuring multiple life-history traits for associating whole-genome transcription responses to lifespan effects of dietary restriction.
In Chapter 8 the acquired insights across the experimental chapters were synthesized, discussing the importance of assessing a broad range of nutrients for the interpretation of any genotypic effect, and in addition discussing the value of measuring multiple life-history traits for genetic associations. In this chapter I also suggested directions for future research in Drosophila and Podospora that may be valuable for further unravelling and understanding the mechanisms of diet responses in other organisms, including in humans.
Vervolgonderzoek emissiearme Lisianthus
Raaphorst, Marcel ; Eveleens, Barbara ; Burg, Rick van der; Schuddebeurs, Lisanne - \ 2017
Bleiswijk : Wageningen UR Glastuinbouw (Rapport GTB 1440) - 30
kasgewassen - kassen - glastuinbouw - snijbloemen - emissiereductie - voedingsstoffen - gewasbescherming - kooldioxide - substraten - cultuur zonder grond - fusarium - bodemschimmels - kunstmatige verlichting - kunstlicht - greenhouse crops - greenhouses - greenhouse horticulture - cut flowers - emission reduction - nutrients - plant protection - carbon dioxide - substrates - soilless culture - fusarium - soil fungi - artificial lighting - artificial light
Lisianthus growers look for methods to minimise the emission of nutrients, crop protection chemicals and CO2. In 2014 and 2015, nine crops with Lisianthus have been tested at the Delphy Improvement Centre. This report describes the four trials that have been carried out in the extended research in 2016. With this extension, a distinction was made between different substrates and intensities of assimilation lighting. In addition to knowledge about light use efficiency, water use, heat use, substrate differences and growth development, these extra crop cycles have brought to light that growing Lisianthus on substrate gives a less resilient plant against soil fungi than was experienced during the first crop cycles.
Mogelijke oorzaken van een dalende opbrengst van witte klaver (Trifolium repens L.) in de tijd
Boer, H.C. de; Eekeren, N. van - \ 2017
Wageningen : Wageningen Livestock Research (Wageningen Livestock Research rapport 1025) - 36
trifolium repens - opbrengsten - reductie - voedingsstoffen - melkveehouderij - trifolium repens - yields - reduction - nutrients - dairy farming
Onderzoek naar oorzaken en oplossingen van grasklaverdaling in de landbouw.
A blooming business : Identifying limits to Lake Taihu's nutrient input
Janssen, Annette B.G. - \ 2017
Wageningen University. Promotor(en): Wolf Mooij, co-promotor(en): J.H. Janse; A.A. van Dam. - Wageningen : Wageningen University - ISBN 9789463431897 - 268
lakes - freshwater ecology - aquatic ecosystems - nutrients - cycling - nutrient flows - biodiversity - algae - models - critical loads - limnology - spatial variation - ecological restoration - china - meren - zoetwaterecologie - aquatische ecosystemen - voedingsstoffen - kringlopen - nutriëntenstromen - biodiversiteit - algen - modellen - critical loads - limnologie - ruimtelijke variatie - ecologisch herstel - china
Last century, Lake Taihu (China) became serious eutrophic due to excessive nutrient input. During the 1980s, the first algal blooms emerged in the lake, reaching disastrous proportions in 2007. During that year, the intake of drinking water had to be shut down and millions of people had to look for an alternative source of drinking water. This raises the question whether such problems can be avoided. Of crucial importance in avoiding and reducing toxic algal blooms is the identification of the maximum nutrient load ecosystems can absorb, while remaining in a good ecological state. In this thesis, I aim to determine the critical nutrient load for Lake Taihu. I approach the search for critical nutrient loads of Lake Taihu in five steps with diversity as an overarching topic throughout this thesis: diversity in lakes, diversity in models, diversity in spatial distribution of nutrient and water sources, diversity in the development of lakes around the earth and finally diversity within specific lakes. From the long list of available models I chose the model PCLake to use in my analysis because it is the most extensively used food web model applied for bifurcation analysis of shallow aquatic ecosystems. The approach has resulted in a range of critical nutrient loads for different parts of Lake Taihu. Furthermore, critical nutrient loads depend on management goals, i.e. the maximum allowable chlorophyll-a concentration. According to the model results, total nutrient loads need to be more than halved to reach chlorophyll-a concentrations of 30-40 μg.L-1 in most sections of the lake. To prevent phytoplankton blooms with 20 μg.L-1 chlorophyll-a throughout Lake Taihu, both phosphorus and nitrogen loads need a nearly 90% reduction. This range contrasts to the single point of recovery that is often found for small shallow lakes. The range in critical nutrient loads found for Lake Taihu can be interpreted as providing a path of recovery for which each step leads to water quality improvement in certain parts of the lake. To reach total recovery, nutrient reduction seems to be the most promising management option.
Verkenning regionale kringlopen : sluiten van nutriëntenkringloop op het niveau van Noordwest-Europa
Leenstra, Ferry ; Vellinga, Theun - \ 2017
Wageningen : Wageningen Livestock Research (Wageningen Livestock Research rapport 987) - 34
voer - voedingsstoffen - dierlijke producten - kringlopen - dierhouderij - dierlijke meststoffen - noord-brabant - noordwest-europa - feeds - nutrients - animal products - cycling - animal husbandry - animal manures - noord-brabant - northwestern europe
Closing the nutrient loops in (peri-)urban farming systems through composting
Nigussie, Abebe - \ 2017
Wageningen University. Promotor(en): Thomas Kuijper; A. de Neergaard, co-promotor(en): S. Bruun. - Wageningen : Wageningen University - ISBN 9789463430050 - 144
urban agriculture - farming systems - nutrients - composting - refuse - sewage - waste treatment - vermicomposting - soil quality - nitrogen - ethiopia - stadslandbouw - bedrijfssystemen - voedingsstoffen - compostering - vuilnis - rioolwater - afvalverwerking - vermicompostering - bodemkwaliteit - stikstof - ethiopië
Organic amendments are used to improve soil fertility and maintain agricultural fields in a productive state. Despite these benefits, the use of organic amendments is limited in many developing countries. The overall objective of this thesis is therefore to provide a better understanding of current waste management practices in developing countries and ensure sustainable crop production via the biotransformation of urban waste into a high-quality soil amendment. First, I aimed at determining the causes for the limited use of organic amendments in small-scale urban farming systems. I interviewed 220 urban farmers in Ethiopia and found that competition for agricultural waste between fuel, feed and soil amendment is a major cause for the limited use of organic amendments. I demonstrated that allocation of agricultural waste for soil amendment is linked with farmers’ livelihood strategies. I also studied variation in compost demand among different farmer groups, and the socio-economic variables which explained these variations.
Gaseous losses of ammonia and greenhouse gas (GHG) emissions occur during composting of nitrogen-rich urban waste. Several technologies could reduce these losses. However, these technologies are inadequate to fit within the broader farming systems because they are expensive. The second aim of this thesis was to develop low-cost methods to mitigate N losses and GHG emissions from composting, while retaining its fertilising value.
Composting by earthworms (vermicomposting) is proposed as a low-cost strategy for minimising N losses and GHG emissions. Using a wide range of substrate qualities (C:N ratio, labile C sources) and other factors (earthworm density, amount of input, and moisture), I showed that vermicomposting reduced N losses and GHG emissions compared with traditional thermophilic composting, but the magnitude of the earthworm effect varied between substrates. Earthworms also change the quantity and composition of dissolved organic carbon during composting. Another low-cost strategy is to delay the addition of N-rich substrates during composting. I demonstrated that addition of nitrogen-rich substrate after the thermophilic phase reduced N losses. Delayed addition of N-rich substrates increased N2O emissions, but reduced CH4 emissions. Delayed addition resulted in compost that was as stable and effective at completely eradicating weed seeds as traditional composting.
In conclusion, urban waste compost should be considered as alternative source for soil amendment, particularly in developing countries with competition for agricultural waste. Technologies such as vermicomposting and delayed addition of N-rich substrate are recommended to increase or maintain the nitrogen content of compost, reduce N losses and mitigate GHG emissions.
Onderzoek Waterkringloopsluiting Tulpenbroeierij : overzicht huidige en toekomstige technieken om waterkringloopsluiting mogelijk te maken
Os, E.A. van; Feenstra, L. ; Ruijven, J. van; Stijger, C.C.M.M. ; Koeman-Stein, N. ; Appelman, W. - \ 2016
Bleiswijk : Wageningen UR Glastuinbouw (Rapport GTB 1394) - 38
tulpen - forceren van planten - glastuinbouw - kasgewassen - afvalwaterbehandeling - afvalwater - recirculatiesystemen - drainagewater - pathogenen - pathogeen eliminatie - gewasbescherming - voedingsstoffen - fenolen - tulips - forcing - greenhouse horticulture - greenhouse crops - waste water treatment - waste water - recirculating systems - drainage water - pathogens - pathogen elimination - plant protection - nutrients - phenols
Closing of the water cycle is required in the forcing of tulips. For growers it is important to know if and how they can eliminate pathogens at an adequate way and which equipment is most suitable to break down plant protection products from waste water. Now participating growers have various equipment for full or partly disinfection. It is recommended to start with good pre-filtration methods to eliminate pathogens which can be fully achieved with UV and ozone, while products such as hydrogen peroxide with additives or chlorite products can be used for an after-effect in the pipe work. Phenolic compounds, released by the roots, can be eliminated with oxidising methods. However efficacy has not been investigated. Purification of discharge water, before entering the waste ditch, will be obliged. New, certified, equipment will come on the market soon. Reuse of discharge water, after pre-fi ltration and disinfection, is also an option.
Improving sustainability of striped catfish (Pangasianodon hypophthalmus) farming in the Mekong Delta, Vietnam through recirculation technology
Nguyen, Nhut - \ 2016
Wageningen University. Promotor(en): Johan Verreth, co-promotor(en): Marc Verdegem. - Wageningen : Wageningen University - ISBN 9789462579194 - 180
fish culture - recirculating aquaculture systems - aquaculture - fishes - nutrients - vietnam - visteelt - recirculatie aquacultuur systemen - aquacultuur - vissen - voedingsstoffen - vietnam
The aim of this thesis was to document improvements in sustainability indicators of striped catfish (Pangasianodon hypophthalmus, Sauvage, 1878) production through the application of recirculation and waste treatment techniques. To be able to document improvements in sustainability, in each system studied the same set of twenty sustainability indicators were measured. Indicators related to the use of fingerlings, water, diesel oil, electricity, labor, chemicals and antibiotics.
Also, indicators related to nutrient utilization efficiencies and waste discharge were monitored. In addition, a sampling scheme, allowing to calculate organic matter, nitrogen, phosphorous and chemical oxygen demand mass balances covering a full production cycle and applicable in different production systems, was developed. Overall, from a sustainability point of view, striped catfish culture in ponds compared well to other important aquaculture species.
Although favorable, it was concluded that water, chemicals and antibiotics use, survival, and the amounts of waste discharged could be further reduced through recirculation and treatment of solid wastes. The realized improvements through RAS technology and waste treatment technology were quantified in lab or pilot scale experiments. Large improvements were realized for water, antibiotic and chemical use, survival, waste discharge and color grade of striped catfish fillets at harvest. In addition, in RAS, utilization efficiencies of nutrients supplied through feeding were improved.
Solid wastes removed from ponds or RAS could be partially re-used by making compost or producing methane for generating electricity. Another approach tested was the integration of a denitrification reactor in the recirculation system, which allowed to decompose solid waste and reduce nitrogen discharge. Denitrification in RAS did not affect fish growth, nutrient retention efficiencies and the quality of the fish fillets produced, and thus also improved sustainability of striped catfish farming.
In conclusion, application of recirculation and waste treatment techniques tested in this thesis improved the sustainability for striped catfish culture. The challenge remains to scale up RAS and waste treatment technology for striped catfish to the production volumes handled in outdoor ponds without raising production costs.
River export of nutrients to the coastal waters of China: the MARINA model to assess sources, effects and solutions
Strokal, Maryna - \ 2016
Wageningen University. Promotor(en): Carolien Kroeze, co-promotor(en): S. Luan; Lin Ma. - Wageningen : Wageningen University - ISBN 9789462579729 - 226
cum laude - nutrients - rivers - coastal water - models - eutrophication - coastal areas - water pollution - china - voedingsstoffen - rivieren - kustwateren - modellen - eutrofiëring - kustgebieden - waterverontreiniging - china
Rivers export increasing amounts of nitrogen (N) and phosphorus (P) to the coastal waters of China. This causes eutrophication problems that can damage living organisms when oxygen levels drop and threaten human health through toxic algae. We know that these problems result from human activities on land such as agriculture and urbanization. However, the relative importance of these human activities for river export of nutrients to Chinese seas is not well studied. There are two important issues that need further investigation: the relative importance of upstream pollution on downstream impacts and the relative importance of typical sources of nutrients in Chinese rivers that are often ignored in existing modeling studies.
My PhD thesis, therefore, aims to better understand trends in river export of nutrients to the coastal waters of China by source from sub-basins, and the associated coastal eutrophication. To this end, I developed the MARINA model: Model to Assess River Inputs of Nutrients to seAs. For this, I used the existing Global NEWS-2 model (Nutrient Export from WaterSheds) as a starting point.
I formulated five sub-objectives to achieve the main objective:
To analyze the original Global NEWS-2 model for river export of nutrients and the associated coastal eutrophication (Chapter 2);
To develop a sub-basin scale modeling approach to account for impacts of upstream human activities on downstream water pollution, taking the Pearl River as an example (Chapter 3);
To quantify the relative share of manure point sources to nutrient inputs to rivers at the sub-basin scale (Chapter 4);
To quantify the relative share of sources to river export of nutrients at the sub-basin scale (Chapter 5);
To explore optimistic futures to reduce river export of nutrients and coastal eutrophication in China (Chapter 6).
The study area includes rivers draining roughly 40% of China. This includes the most densely populated areas, and areas with intensive economic activities. The rivers include the Yangtze (Changjiang), Yellow (Huanghe), Pearl, Huai, Hai and Liao. In the MARINA model, the drainage areas of the large Yangtze, Yellow and Pearl rivers are divided into up-, middle- and downstream sub-basins. The principle of the sub-basin approach of MARINA is that nutrients from human activities are transported by tributaries to outlets of sub-basins and then to the river mouth (coastal waters) through the main channel. The model takes into account nutrients that are partly lost or retained during transport towards the river mouth. The model quantifies river export of nutrients by source from sub-basins for 1970, 2000 and 2050.
The main six findings of the MARINA results for China are:
Finding 1: Dissolved N and P export by Chinese rivers increased by a factor of 2-8 between 1970 and 2000;
Finding 2: The potential for coastal eutrophication was low in 1970 and high in 2000 in China;
Finding 3: Most dissolved N and P in Chinese seas is from middlestream and downstream human activities;
Finding 4: Manure point sources are responsible for 20-80% of dissolved N and P in Chinese rivers;
Finding 5: In the future, river export of nutrients may increase in the Global Orchestration (GO) scenario of the Millennium Ecosystem Assessment. Current policy plans (CP scenario) may not sufficient to avoid this increase;
Finding 6: In optimistic scenarios (OPT-1 and OPT-2), the potential for coastal eutrophication is low in 2050, mainly as a result of assumed full implementation of: (1) high recycling rates of animal manure (OPT-1 and OPT-2), and (2) high efficiencies of nutrient removal in sewage systems (OPT-2, see Figure 1).
Figure 1. Illustration of future scenarios for coastal water quality in China. GO is Global Orchestration of the Millennium Ecosystem Assessment and assumes environmental actions that are either absent or ineffective in reducing water pollution. CP is based on GO, but incorporates the “Zero Growth in Synthetic Fertilizers after 2020” policy. OPT-1 and OP-2 are optimistic scenarios that assume high nutrient use efficiencies in agriculture (OPT-1, OPT-2) and sewage (OPT-2).
My PhD thesis reveals novel insights for effective environmental policies in China. It shows the importance of manure point sources in water pollution by nutrients. Clearly, managing this source will likely reduce coastal eutrophication in the future. Furthermore, the implementation of advanced technologies is essential when dealing with urban pollution. My PhD thesis may also be useful for other world regions with similar environmental problems as in China. The new, sub-basin scale MARINA model is rather transparent and thus can be applied to other large, data-poor basins that may benefit from the allocation of effective management options. With this I hope to contribute to future availability of sufficiently clean water for next generations, not only in China, but also in other world regions.
Agricultural intensification in Nepal, with particular reference to systems of rice intensification
Uprety, Rajendra - \ 2016
Wageningen University. Promotor(en): Thomas Kuijper, co-promotor(en): Harro Maat. - Wageningen : Wageningen University - ISBN 9789462579651 - 190
rice - oryza sativa - nepal - asia - south asia - intensification - livelihoods - livelihood strategies - farming systems - farming - crop management - fertilizers - nutrients - irrigation - varieties - rijst - oryza sativa - nepal - azië - zuid-azië - intensivering - middelen van bestaan - strategieën voor levensonderhoud - bedrijfssystemen - landbouw bedrijven - gewasteelt - kunstmeststoffen - voedingsstoffen - irrigatie - rassen (planten)
This thesis deals with agricultural intensification in Nepal. The initial focus of the study was the System of Rice Intensification (SRI), as introduced in Nepal from 2001. The multiple factors affecting SRI adoption, modification and dissemination together with the option to apply SRI in different combinations of its components result in a variety of SRI applications. For the same reason the effect of SRI on overall agricultural and livelihood development of Nepalese farmers has to be evaluated within the variety of farming systems in which it is applied.
Despite government policies to promote rice cultivation, national rice production is declining. Farmer livelihood strategies, as reflected in rice farming systems, and field management strategies were influenced by several agro-ecological and socio-economic factors. Livelihood and field management strategies of rice farmers are interconnected. In the study presented here four livelihood strategies and three kinds of field management strategies are distinguished. Two livelihood strategies can be characterized as more intensive and more productive; the other two are less intensive and less productive. Livelihood strategies are more family resource-based strategies, while farmers’ field management strategies are more context-dependent. Field management strategies were characterized by forms of nutrient management. Intensive management strategies had most similarities with SRI. But rice intensification is not achievable as a general strategy.
Government policies (fertiliser subsidies) encourage increased fertiliser use. Study results didn't show any significant effect of volume of fertilisers on rice yield but the combined use of organic manure and mineral fertilisers resulted in the highest average rice yields. Irrigation management is another important factor for rice production. Field management is influenced by the reliability of water which was better in farmers' managed irrigation system. Choice of rice varieties influenced the overall rice farming system and cropping intensity and preference of varieties for rice cultivation by scientists and by farmers were different in eastern Nepal. Most popular varieties were those not recommended by science and policy and were disseminated farmer to farmer.
The introduction of SRI in Morang district resulted in several changes in rice farming, but only part of the farmers have adopted such technologies, and adoption has been only in part of their fields. Other farmers have incorporated some SRI practices in their conventional practices. After the introduction of SRI, farmers further tested, re-packaged or hybridized SRI methods to make SRI ideas suitable for their agro-ecological and socio-economic environments. In order to reform Nepalese rice farming, we need to recognize that different farmers, with different livelihood strategies, and with access to different kinds of fields, need different forms for agricultural intensification. High-intensive farmers prefer to use modified SRI methods where there is good irrigation and drainage facilities. There are many possibilities for improvement of the existing nutrient management practices of rice farmers in Nepal. Nutrient management will be useful to increase rice production because the majority of farmers currently use fertilisers non-judiciously. The SRI-recommended practices (younger seedlings, early weeding, use of organic manure, and alternate wetting and drying (AWD) irrigation) will be useful to improve the nutrient use efficiency of rice farmers. Cost-reduction strategies and less labour-intensive cultivation practices will be appropriate options to improve existing rice farming system of Nepal. Participatory cultivar selection and dissemination will be better strategies to introduce new, promising rice cultivars among rice farmers.
Landbouw en de KRW-opgave voor nutriënten in regionale wateren : het aandeel van landbouw in de KRW-opgave, de kosten van enkele maatregelen en de effecten ervan op de uit- en afspoeling uit landbouwgronden
Groenendijk, Piet ; Boekel, Erwin van; Renaud, Leo ; Greijdanus, Auke ; Michels, Rolf ; Koeijer, Tanja de - \ 2016
Wageningen : Wageningen Environmental Research (Wageningen Environmental Research rapport 2749) - 149
kaderrichtlijn water - voedingsstoffen - oppervlaktewater - oppervlaktewaterkwaliteit - stikstof - fosfaten - uitspoelen - landbouw - oppervlakkige afvoer - water framework directive - nutrients - surface water - surface water quality - nitrogen - phosphates - leaching - agriculture - runoff
Het doel van de Europese Kaderrichtlijn Water is duurzame bescherming van ecosystemen en watervoorraden. Een deel van de regionale waterlichamen voldoet nog niet aan de normen voor stikstof- en fosforconcentraties die behoren bij een goede ecologische toestand. Om af te wegen welke maatregelen kunnen bijdragen aan het realiseren van de KRW-doelen, is inzicht nodig in de herkomst van stikstof en fosfor in het oppervlaktewater. In dit onderzoek is het aandeel van de landbouw in de overschrijding van de normen voor de stikstof- en de fosforconcentratie in regionale waterlichamen berekend met modellen. In deze analyse zijn verschillende brontermen onderscheiden die niet afzonderlijk te meten zijn (kwel, nalevering bodem, na-ijling uit overschotten in het verleden) en alleen met modellen zijn te berekenen. Vervolgens zijn van een viertal maatregelen (vervanging uitspoelingsgevoelige gewassen in het zuidelijke zandgebied, bodemverbetering, verbetering nutriëntenbenutting en drainage) de effecten op de uit- en afspoeling van stikstof en fosfor geschat. Ook zijn gevolgen voor het gebiedsgemiddelde financieel saldo geschat. Om te voldoen aan de normen voor stikstof- en fosforconcentraties in regionale waterlichamen, moet de uit- en afspoeling uit landbouwgronden landelijk gemiddeld met respectievelijk 12─17% en 12–38% worden verminderd, afhankelijk van de wijze waarop de uit- en afspoeling wordt vertaald naar een aandeel in de overschrijding van de concentratienormen. Tussen regio’s doen zich grote verschillen voor. Door een combinatie van de doorgerekende maatregelen lijkt in de zandgebieden een groot deel van de opgave voor reductie van de stikstofuitspoeling gerealiseerd te kunnen worden, terwijl de opgave voor het reduceren van de uit- en afspoeling van fosfor slechts voor een klein deel gerealiseerd kan worden met deze maatregelen. Voor fosfor zijn andere en/of aanvullende maatregelen nodig die voorkomen dat de opgehoopte voorraad fosfaat in de bovengrond kan uitspoelen naar het oppervlaktewater en/of maatregelen met een zuiverende werking in het oppervlaktewater.
Vergelijking tussen gangbaar en emissieloos teeltsysteem : waterefficiënte Emissieloze Kas
Os, Erik van; Ruijven, Jim van; Janse, Jan ; Beerling, Ellen ; Staaij, Marieke van der; Kaarsemaker, Ruud - \ 2016
Bleiswijk : Wageningen UR Glastuinbouw (Rapport GTB 1406) - 62
paprika's - capsicum annuum - teelt onder bescherming - kasgewassen - glasgroenten - glastuinbouw - emissiereductie - emissie - stikstof - fosfaat - voedingsstoffen - pesticiden - sweet peppers - capsicum annuum - protected cultivation - greenhouse crops - greenhouse vegetables - greenhouse horticulture - emission reduction - emission - nitrogen - phosphate - nutrients - pesticides
Stricter legislation forces growers to decrease discharge of water with nitrogen, phosphate and plant protection products. A demonstration was set-up to compare emission free cultivation with a traditional growing method. Goal was to achieve a comparable yield and quality. Essential differences were disinfection of rainwater, ring pipes for drip irrigation, no discharge of first drain in cultivation, a filter without rinsing and weekly analysis of the nutrient solution. It appeared that production and quality were similar as were other climate parameters. Water use was higher in emission free crop, discharge was 4% in traditional crop realizing 152 kg N discharge per ha per year, which was more than the allowed 133 kg. Specific end cultivation strategy is useful to decrease nitrogen and phosphate stored in slabs and stock containers.
Belasting van waterlichamen in de Krimpenerwaard met stikstof en fosfor
Schipper, P.N.M. ; Hendriks, R.F.A. ; Massop, H.T.L. ; Boekel, E.M.P.M. - \ 2016
Wageningen : Wageningen Environmental Research (Wageningen Environmental Research rapport 2738) - 75
waterbalans - voedingsstoffenbalans - voedingsstoffen - drainage - oppervlaktewater - modellen - krimpenerwaard - water balance - nutrient balance - nutrients - drainage - surface water - models - krimpenerwaard
Om inzicht te krijgen in de herkomst van nutriënten in de Krimpenerwaard is een modelinstrumentarium gebouwd dat de waterbalans en nutriëntenbalans in beeld brengt voor vier afwateringsgebieden binnen de Krimpenerwaard. Vervolgens is de beïnvloedbaarheid van bronnen afgeleid conform de werkwijze die wordt toegepast door de Nutriëntenwerkgroep Rijn-West. Uit de resultaten blijkt dat er tussen de vier afwateringsgebieden duidelijke verschillen zijn in herkomst van de stikstof- en fosforbelasting van het oppervlaktewater en de beïnvloedbaarheid hiervan. In de Nesse is het grootste gedeelte moeilijk/niet beïnvloedbaar door de relatief hoge bijdrage van de uit- en afspoeling vanuit natuurgronden, voor de Krimpenerwaard is het grootste gedeelte beïnvloedbaar met een direct effect door de aanvoer van nutriënten via inlaatwater. Met het modelinstrumentarium is verder verkend wat het effect is van een aantal scenario’s op de nutriëntenbelasting naar het oppervlaktewater. In het autonome scenario daalt de af- en uitspoeling van stikstof in de periode 2015–2029 t.o.v. de huidige situatie (2014) met ca. 15%, de fosforbelasting verandert door de sterke buffering van de bodem bijna niet of neemt zelfs wat toe. Door verruiming van het areaal natuur, aanleg van onderwaterdrainage op koopveengronden en lagere bodemoverschotten door kringlooplandbouw, neemt de af- en uitspoeling ten opzichte van de autonome situatie met ca. 10 à 20% af.
Goed Gietwater Werkpakket 3, taak 2 Industrieelonderzoek naar waterkringloopsluiting in opkweekbedrijven
Appelman, W.A.J. ; Brouwer, J.G.H. ; Blok, C. ; Maas, A.A. van der; Staaij, M. van der; Beerling, E.A.M. ; Meeuwsen, B. - \ 2016
Zeist : TNO - 61
waterverontreiniging - emissie - emissiereductie - glastuinbouw - voedingsstoffen - waterbeheer - afvalwater - hergebruik van water - water pollution - emission - emission reduction - greenhouse horticulture - nutrients - water management - waste water - water reuse
Efficiënt omgaan met water speelt voor biologische en niet-biologische opkweekbedrijven van plantmateriaal een steeds grotere rol. Toenemende en strengere eisen met betrekking tot lozing van water en de emissie van nutriënten en gewasbeschermingsmiddelen naar het oppervlaktewater vragen nieuwe maatregelen. Overheden en de glastuinbouwsector hebben als doelstelling om de emissies van de glastuinbouwsector via het waterspoor in 2027 nagenoeg tot nul te reduceren waarbij er in 2018 al maatregelen moeten worden genomen met betrekking tot gewasbeschermingsmiddelen. Het doel van het Goed Gietwater project is om middels industrieel onderzoek te komen tot een vergaande waterkringloopsluiting in de opkweeksector. Met een geoptimaliseerd watermanagement en behandeling van het overtollige gietwater kunnen water en nutriënten worden teruggewonnen en emissies van nutriënten en gewasbeschermingsmiddelen naar het oppervlaktewater worden voorkomen.
Bronnen van nutriënten in het oppervlaktewater in het beheergebied van Wetterskip Fryslân : studie naar de herkomst en beïnvloedbaarheid van stikstof en fosfor in het oppervlaktewater voor zes polders in het beheergebied van Wetterskip Fryslân
Boekel, E.M.P.M. van; Groenendijk, P. ; Renaud, L.V. - \ 2016
Wageningen : Alterra, Wageningen-UR (Alterra-rapport 2727) - 50
oppervlaktewater - voedingsstoffen - stikstof - fosfor - modellen - oppervlaktewaterkwaliteit - mestbeleid - friesland - surface water - nutrients - nitrogen - phosphorus - models - surface water quality - manure policy - friesland
Om inzicht te krijgen in de herkomst en beïnvloedbaarheid van stikstof en fosfor in het oppervlaktewater in het beheergebied van Wetterskip Fryslân zijn met de ECHO-methodiek stofbalansen opgesteld voor een zestal polders. De beïnvloedbaarheid van bronnen is afgeleid conform de werkwijze die wordt toegepast door de Nutriëntenwerkgroep Rijn-West. Daarnaast is het effect van het mestbeleid op de nutriëntenbelasting van het oppervlaktewater in beeld gebracht op basis van uit het landelijke STONE-model afgeleide resultaten voor de uitspoeling bij gebruiksnormen van het 5e Nitraat Actieprogramma (5e NAP). Uit de resultaten blijkt dat het grootste deel (46 −74%) van de stikstof- en fosforbelasting van het oppervlaktewater afkomstig is van beïnvloedbare bronnen (actuele bemesting en nalevering bodem) met een effect op de korte en lange termijn. Bronnen met een direct effect (voornamelijk inlaatwater) dragen voor 5 − 45% bij aan de totale nutriëntenbelasting. De stikstofbelasting van het oppervlaktewater is voor 7 − 22% afkomstig van niet of moeilijk te beïnvloeden bronnen, voor fosfor ligt dit tussen 1 en 12%. Het doorrekenen van het mestbeleid (5e NAP) resulteert in een reductie van de uit- en afspoeling tussen 1,9 en 9,1%. Voor de totale nutriëntenbelasting ligt de reductie als gevolg van de maatregelen in het 5e NAP tussen 1,1 en 7,7%.
Mining into interspecific bacterial interactions
Tyc, Olaf - \ 2016
Wageningen University. Promotor(en): Wietse de Boer, co-promotor(en): Paolina Garbeva. - Wageningen : Wageningen University - ISBN 9789462578340 - 234
soil bacteria - secondary metabolites - microbial interactions - antibiotics - nutrients - bodembacteriën - secundaire metabolieten - microbiële interacties - antibiotica - voedingsstoffen
In terrestrial ecosystems bacteria live in close proximity with many different microbial species and form complex multi-species networks. Within those networks bacteria are constantly interacting with each other and produce a plethora of secondary metabolites like antibiotics, enzymes, volatiles and other compounds from diverse chemical classes. Several independent studies revealed that the production of secondary metabolites by soil bacteria can be influenced by the interaction with other microorganisms in their vicinity.
In this thesis we show how interspecific interactions between soil bacteria influence the production of soluble and volatile secondary metabolites, gene expression and fitness. To elucidate the effect of interspecific interactions on antimicrobial activity in soil bacteria a high-through-put screening method was developed and applied on a collection of 146 rhizobacterial isolates obtained from similar habitats. In addition we examined if the production of volatile organic compounds is influenced by interspecific interactions. Thus, the identity and antimicrobial activity of volatiles produced by bacteria cultivated in monoculture as well in interaction were examined. Furthermore a sand microcosm approach was applied to investigate how Pseudomonas fluorescens strain Pf0-1 responded to the presence of monocultures and mixtures of a Gram-negative (Pedobacter sp. V48) and a Gram-positive (Bacillus sp. V102) bacterial strain under two nutritional conditions.
The interaction between a gram-negative Burkholderia and a gram-positive Paenibacillus isolate was subjected to detailed metabolome, volatolome and transcriptome analysis. One distinct volatile and one non-volatile compound produced only during interspecific interaction but not in the monoculture were identified. The activity of the interacting bacteria and the compounds produced during interaction were tested against a range of human and plant pathogens.
In summary, this thesis extends the knowledge about the effect of interspecific bacterial interactions on secondary metabolites production (soluble and volatiles), gene expression and fitness in bacteria. The exploitation of such bacterial interspecific interactions can be an important “tool” for the discovery of novel antimicrobial and agro-chemical compounds. The obtained knowledge can help in selecting the right players in synthetic communities that fulfil important ecosystem services like disease suppression in agricultural crop systems.
Nieuwe voedingsschema’s phalaenopsis voor veranderde teeltwijze : recirculatie, productieverhoging, teeltverkorting
Kromwijk, Arca ; Blok, Chris - \ 2016
horticulture - greenhouse horticulture - pot plants - phalaenopsis - recirculating systems - cropping systems - nutrients - crop production - urea - sodium - zinc - crop growth stage - agricultural research
De teelt van phalaenopsis is in de afgelopen vijftien jaar zo sterk veranderd, dat de praktijk niet goed meer uit de voeten kon met de voedingsschema’s uit de jaren negentig. Onderzoekers van Wageningen UR Glastuinbouw hebben daarom nieuwe schema’s ontwikkeld, die inmiddels op grote schaal toepassing vinden. Het gaat om schema’s voor opkweek (vegetatief) en afkweek (generatief) in bedrijfssituaties met en zonder recirculatie.
Recovery of nutrients from biogas digestate with biochar and clinoptilolite
Kocaturk, N.P. - \ 2016
Wageningen University. Promotor(en): L. Stoumann Jensen; Lijbert Brussaard, co-promotor(en): S. Bruun; Kor Zwart. - Wageningen : Wageningen University - ISBN 9789462578234 - 130
biochar - clinoptilolite - digestate - nutrients - waste management - fractionation - growth - biochar - clinoptiloliet - digestaat - voedingsstoffen - afvalbeheer - fractionering - groei
The liquid fraction of digestate contains nutrients which makes it a valuable fertiliser in agricultural crop production systems. However, direct application of digestate may raise practical and environmental problems. Therefore, processes to concentrate nutrients have been proposed aiming not only to treat the liquid fraction of digestate to overcome the problems related to direct application, but also to recover the nutrients of which natural reserves are being depleted such as phosphorus and potassium.
In this thesis, the focus was on the evaluation of the use of clinoptilolite and biochar for nutrient recovery from the liquid fraction of digestate; and to investigate the further use of these nutrient-enriched materials as fertiliser.
This thesis showed that sorption with clinoptilolite and biochar can be a promising technology to recover nutrients from liquid fraction of digestate. Using biochar and clinoptilolite provides concentration of nutrients and, thereby, volume reduction which allows for savings on storage, transport and application of the voluminous liquid fraction of digestate. The end-products are digestate-enriched clinoptilolite and enriched biochar can act as N fertilisers.
Are all eggs equal? : embryonic development and nutrient metabolism in chicken eggs of different origins
Nangsuay, A. - \ 2016
Wageningen University. Promotor(en): Bas Kemp, co-promotor(en): Henry van den Brand; R. Meijerhof. - Wageningen : Wageningen University - ISBN 9789462577749 - 213
eggs - hens - broilers - characteristics - strains - embryonic development - nutrients - metabolism - hatcheries - poultry - nutrition physiology - eieren - hennen - vleeskuikens - karakteristieken - stammen (biologisch) - embryonale ontwikkeling - voedingsstoffen - metabolisme - broedinstallaties - pluimvee - voedingsfysiologie
Hatching eggs, supplied to hatcheries are originating from different origins varying in breed, strain, and breeder age. These hatching eggs can be different in size, composition and eggshell properties, which might influence nutrient and O2 availability and consequently could affect embryonic development and nutrient metabolism. The aim of this thesis was therefore 1) to investigate effects of egg origin on nutrient and O2 availability, 2) to investigate effects of egg origins on nutrient metabolism and embryonic development and 3) to investigate consequences of different egg origins on the incubation process and hatching characteristics. In five studies, effects of different egg origins on nutrient and O2 availability, nutrient metabolism, embryo development and hatching characteristics were investigated. The first and second study focused on breeder age and egg size. The third study on breed; broilers and layers. The fourth study on broiler strain and the fifth study on breeder age, strain and eggshell temperature (EST). The overall findings in this thesis suggest that hatching eggs from different origins are not equal in availability of nutrients and O2. Nutrient availability is altered through variation in yolk size, especially by the effects of breeder age and breed. O2 availability is altered by differences in eggshell properties, which is influenced by especially breed and broiler strain. The availability of both nutrients and O2 plays a role on nutrient metabolism measured as embryonic heat production (HP) and consequently on embryonic development. Between incubation day (E) E7 and E14, both nutrient and O2 availability might affect nutrient metabolism as shown in the results of the broiler and layer comparison. Between E14 and hatching, the availability of O2 becomes the most determinant factor for nutrient metabolism and consequently for embryonic development. An increase in EST from 37.8 to 38.9°C from E7 onward resulted in an acceleration of nutrient metabolism and embryonic development until E16, but thereafter a high EST resulted in reduced yolk free body mass development. Embryos with an accelerated metabolic speed at an early stage of incubation, caused by an increased EST, might reach limited O2 availability at a higher magnitude than the embryos at a normal EST. As a result, nutrient metabolism is restricted and embryonic development is depressed. It can be concluded that not only the HP, but also the availability of O2 is crucial to be taken into account for developing incubator temperature. The principle is to obtain an optimal EST, which could maintain the balance between O2 requirement (driven by nutrient metabolism) and O2 availability for a continuing optimal nutrient metabolism to generate sufficient energy for embryonic development throughout incubation.
Recirculatie bij snij-amaryllis (Hippeastrum) in drie teeltjaren (2013-2015) : Behoud plantgezondheid en voorkomen groeiremming bij hergebruik drainwater
Kromwijk, J.A.M. ; Burg, R. van der; Nijs, L. ; Overkleeft, J. ; Eveleens-Clark, B.A. ; Blok, C. ; Os, E.A. van; Baar, P.H. van; Grootscholten, M. ; Woets, F. - \ 2016
Bleiswijk : Wageningen UR Glastuinbouw (Rapport GTB 1398) - 88
amaryllis - glastuinbouw - kasgewassen - kasproeven - teelt onder bescherming - emissie - hergebruik van water - afvalwaterbehandeling - afvalwater - recirculatiesystemen - lycorine - stikstof - voedingsstoffen - kunstmeststoffen - amaryllis - greenhouse horticulture - greenhouse crops - greenhouse experiments - protected cultivation - emission - water reuse - waste water treatment - waste water - recirculating systems - lycorine - nitrogen - nutrients - fertilizers
In the Netherlands most crops grown in greenhouses reuse drain water. However, in the cultivation of amaryllis
cut flowers (Hippeastrum) little drainage water is being reused so far because of strong suspicions of inhibitory
substances in the drainage water. To reduce the emission of nutrients to the environment an experiment was
started on request of the amaryllis growers. In a greenhouse experiment drainage water of amaryllis was treated
with advanced oxidation and reused. This was compared with the reuse of drainage water treated with an UV
disinfector and a control treatment without the reuse of drainage water. In three years of cultivation, there was
no difference in production and no adverse effects were seen in crop growth. This research was funded by the
Dutch amaryllis growers, the ‘Topsector Tuinbouw en Uitgangsmaterialen’, the Product Board for Horticulture, the
project ‘Samenwerken aan Vaardigheden’ and Koppert.