Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Virulence contribution and recognition of homologs of the Verticillium dahliae effector Ave1
    Boshoven, Jordi C. - \ 2017
    Wageningen University. Promotor(en): B.P.H.J. Thomma; P.J.G.M. de Wit. - Wageningen : Wageningen University - ISBN 9789463436441 - 183
    verticillium dahliae - plant pathogenic fungi - plant pathogens - disease resistance - virulence factors - virulence - immunity - host parasite relationships - plant-microbe interactions - symbiosis - mutagenesis - resistance breeding - verticillium dahliae - plantenziekteverwekkende schimmels - plantenziekteverwekkers - ziekteresistentie - virulente factoren - virulentie - immuniteit - gastheer parasiet relaties - plant-microbe interacties - symbiose - mutagenese - resistentieveredeling

    Disease resistance in crops is an important aspect of securing global food security. Resistant plants carry immune receptors that sense pathogen invasion often through the recognition of important pathogen virulence factors, known as effectors. Thus, identification and characterization of effectors is important for the fundamental understanding of virulence mechanisms and to aid in resistance breeding. In this thesis the VdAve1 effector of the soil-borne fungal pathogen Verticillium dahliae is studied that is recognized by tomato immune receptor Ve1. Homologs were found in other plant pathogens and the role in virulence in these pathogens was analyzed. Ave1 homologs are differentially recognized by Ve1 and with a combination of domain swaps and truncations a surface exposed patch was identified that contributes to the recognition by Ve1. Knowledge of specific effector-receptor combinations and knowledge of effectors in general can be exploited to aid in breeding for durable resistance in crops.

    Recognition of Verticillium effector Ave1 by tomato immune receptor Ve1 mediates Verticillium resistance in diverse plant species
    Song, Yin - \ 2017
    Wageningen University. Promotor(en): B.P.H.J. Thomma; P.J.G.M. de Wit. - Wageningen : Wageningen University - ISBN 9789463437950 - 231
    disease resistance - defence mechanisms - immunity - plant-microbe interactions - plant pathogens - verticillium dahliae - verticillium - tomatoes - solanum lycopersicum - receptors - genes - tobacco - nicotiana glutinosa - potatoes - solanum tuberosum - solanum torvum - humulus lupulus - cotton - gossypium hirsutum - transgenic plants - arabidopsis thaliana - ziekteresistentie - verdedigingsmechanismen - immuniteit - plant-microbe interacties - plantenziekteverwekkers - verticillium dahliae - verticillium - tomaten - solanum lycopersicum - receptoren - genen - tabak - nicotiana glutinosa - aardappelen - solanum tuberosum - solanum torvum - humulus lupulus - katoen - gossypium hirsutum - transgene planten - arabidopsis thaliana

    Plant-pathogenic microbes secrete effector molecules to establish disease on their hosts, whereas plants in turn employ immune receptors to try and intercept such effectors in order to prevent pathogen colonization. Based on structure and subcellular location, immune receptors fall into two major classes; cell surface-localized receptors that comprise receptor kinases (RKs) and receptor-like proteins (RLPs) that monitor the extracellular space, and cytoplasm-localized nucleotide-binding domain leucine-rich repeat receptors (NLRs) that survey the intracellular environment. Race-specific resistance to Verticillium wilt in tomato (Solanum lycopersicum) is governed by the tomato extracellular leucine-rich repeat (eLRR)-containing RLP-type cell surface receptor Ve1 upon recognition of the effector protein Ave1 that is secreted by race 1 strains of the soil-borne vascular wilt Verticillium dahliae. Homologues of V. dahliae Ave1 (VdAve1) are found in plants and in a number of plant pathogenic microbes, and some of these VdAve1 homologues are recognized by tomato Ve1. The research presented in this thesis aims to characterize the role of the tomato cell surface-localized immune receptor Ve1, and its homologues in other diverse plant species, in Verticillium wilt resistance.

    Evasion of chitin-triggered immunity by fungal plant pathogens
    Rövenich, Hanna J. - \ 2017
    Wageningen University. Promotor(en): B.P.H.J. Thomma; P.J.G.M. de Wit. - Wageningen : Wageningen University - ISBN 9789463436137 - 133
    plant-microbe interactions - immunity - receptors - verticillium dahliae - cladosporium - plant pathogens - chitin - arabidopsis thaliana - fungi - plant-microbe interacties - immuniteit - receptoren - verticillium dahliae - cladosporium - plantenziekteverwekkers - chitine - arabidopsis thaliana - schimmels

    Plants establish intricate relationships with microorganisms that range from mutualistic to pathogenic. In order to prevent colonization by potentially harmful microbes, plant hosts employ surface-localized receptor molecules that perceive ligands, which are either microbe-derived or result from microbe-mediated plant manipulation. This recognition ultimately leads to the activation of host immunity. In order to circumvent recognition or suppress immune responses, microbes secrete effector proteins that deregulate host physiological processes. While the number of identified putative effectors has rapidly increased in recent years, their functions and the mechanisms governing their recognition have largely remained unexplored. To enhance our understanding of the molecular interplay between host and microbe, the work presented here was designed to identify further components involved in the recognition of the two fungal pathogens Verticillium dahliae and Cladosporium fulvum, as well as to characterize the functions of effector proteins produced by these pathogens during tomato infection.

    Nieuwe methoden in plantversterking tegen ondergrondse ziekten en plagen : gebruik van lokaal aanwezige antagonisten uit groeisubstraat en plant
    Wurff, Andre van der; Streminska, M.A. ; Boer, F.A. de; Bruyant, Ewen ; Cuesta Arenas, Y. - \ 2016
    Bleiswijk : Wageningen University & Research, BU Glastuinbouw (Rapport GTB 1427) - 42
    kasgewassen - kassen - glastuinbouw - gewasbescherming - plantenziekteverwekkers - antagonisten - bacteriën - verdediging - verdedigingsmechanismen - endofyten - pythium ultimum - meloidogyne - rhizobium rhizogenes - fusarium oxysporum - fusarium - micro-organismen - proteïnaseremmers - bèta-glucanase - chitinase - greenhouse crops - greenhouses - greenhouse horticulture - plant protection - plant pathogens - antagonists - bacteria - defence - defence mechanisms - endophytes - pythium ultimum - meloidogyne - rhizobium rhizogenes - fusarium oxysporum - fusarium - microorganisms - proteinase inhibitors - beta-glucanase - chitinase
    Within this project, two new methods of the control of pathogens were investigated. New methods are: a. use of local bacteria that are isolated from soils or growing substrates; and b. bacteria that are present within the plant. By using local antagonists, already present in growing substrates or within plants in the greenhouse, the chance is higher that antagonist can be successfully used against local pathogens. Bacteria that were isolated from soil of growers were assessed on their antagonistic potential in lab trials against Pythium ultimum, Meloidogyne spp. and Rhizobium rhizogenes and Fusarium solani and F. oxysporum. Finally, the effect of antagonists against Pythium and Meloidogyne was evaluated in pot trials in the greenhouse. All antagonists diminished brown colourization symptoms in stems caused by Pythium. Alcaligenues sp., Bacillus sp. en two unidentified species diminished root damage and Alcaligenues sp. as well as Bacillus also reduced also the number of offspring of Meloidogyne spp. within the roots. The use of local microorganisms offers a sustainable-, new solution to control pathogens. In this study, it was shown that Proteinase inhibitor 2 (PINII), Glucanase (LeGluB) and Chitinase (LeChi3) can be used in tomato to investigate the influence of antagonists or endophytes on the plant defence.
    Enrichment proteomics challenges and perspectives : analysis of the N-glycoproteome and plasma membrane proteome in glycosylation mutants and plant-pathogen interactions
    Song, Wei - \ 2016
    Wageningen University. Promotor(en): Harro Bouwmeester, co-promotor(en): Sander van der Krol; Twan America. - Wageningen : Wageningen University - ISBN 9789462578722 - 172
    proteomics - glycoproteins - arabidopsis - plant-animal interactions - plant pathogens - plasma membranes - eiwitexpressieanalyse - glycoproteïnen - arabidopsis - plant-dier interacties - plantenziekteverwekkers - plasmamembranen

    This thesis is based on two technology projects from the Centre for BioSystems Genomics (CBSG), entitled ‘Comparative proteomics on Plant Pathogen interactions through enrichment of the N-glycoproteome and tagged-glycoproteome’ (TD7) and ‘Plasma Membrane proteomics for Plant Pathogen interactions’ (TD5). In the former project we developed the protocol for isolation, identification and quantification of N-linked glycoproteins from plants and used it to obtain a comprehensive inventory of glycan-occupancy of Arabidopsis glycoproteins. In the second project, a protocol for the enrichment of plasma membrane (PM) fraction from plant material was developed and applied to study the role of the PM proteome in the interaction of plants with the plant pathogen Phytophthora infestans. Combined these activities have resulted in a thesis devoted to technical developments in label-free comparative enrichment proteomics, with validation in a number of different biological systems.

    Screening of the COGEM lists of non-pathogenic bacteria and fungi for postharvest diseases and plant pathogens
    Vlugt, R.A.A. van der; Verbeek, M. ; Molhoek, W. ; Stevens, L. - \ 2015
    Wageningen : Wageningen UR, Business Unit Biointeractions and Plant Heath (CGM onderzoeksrapport 2016-06) - 109
    plantenziekteverwekkers - plantenziekten - bederf na de oogst - plantenziekteverwekkende bacteriën - plantenziekteverwekkende schimmels - screenen - plant pathogens - plant diseases - postharvest decay - plant pathogenic bacteria - plant pathogenic fungi - screening
    Extensive literature searches soil and growing media inventories : (RC/EFSA/PLH/2013/01-SC1)
    Bremmer, J. ; Holeva, M. ; Breukers, M.L.H. ; Brouwer, J.H.D. - \ 2015
    Brussels : IBF International Consulting (EFSA supporting publication 2015- EN-834) - 49
    bodem - groeimedia - basisproducten - plantenziekteverwekkers - plantenplagen - onkruiden - gastheerreeks - inventarisaties - risicoschatting - soil - growing media - commodities - plant pathogens - plant pests - weeds - host range - inventories - risk assessment
    In this project two inventories by means of extensive literature searches have been executed: Inventory 1 of all types of soil and growing media (if relevant components thereof) to be elaborated considering (i) the soil and growing medium imported as commodities (i.e. not in association with plants intended for planting), (ii) the soil and growing medium attached to plants for planting, and (iii) the soil and growing medium attached as a contaminant to imported goods (ranging from ware potatoes to agricultural machinery). Inventory 2, based on interceptions data and scientific and technical literature, of plant pests, plant diseases and weeds that can be associated with soil and growing medium (if relevant components thereof). Execution of Inventory 1 resulted in a list of 956 soils and growing media. Execution of Inventory 2 resulted in Excel tables for each taxonomic group separately in which the pests and diseases are linked to the soil and growing media. Additional information has been provided for processes applied to produce growing media and the impact on harmful organisms and private certification schemes applied in Europe to control the trade and use of growing media.
    Suppression of soil-borne plant pathogens
    Agtmaal, M. van - \ 2015
    Wageningen University. Promotor(en): Wietse de Boer; J.A. van Veen. - Wageningen : Wageningen University - ISBN 9789462572911 - 151
    plantenziekteverwekkers - bodempathogenen - bodembacteriën - desinfecteren - landbouwgronden - modellen - rizosfeer - ziektewerende gronden - plant pathogens - soilborne pathogens - soil bacteria - disinfestation - agricultural soils - models - rhizosphere - suppressive soils

    Soil borne plant pathogens considerably reduce crop yields worldwide and are difficult to control due to their ”masked” occurrence in the heterogeneous soil environment. This hampers the efficacy of chemical - and microbiological control agents. Outbreaks of crop diseases are not only dependent on the presence of pathogen propagules in the soil, but are also influenced by soil-related properties like physico-chemical characteristics, microbial activity and community composition. Strong competition for limited available carbon substrates restricts or prevents germination and pre-infective growth of pathogens. This competition can occur directly by rapid exploitation of substrates, so called resource competition, or indirectly via inhibitory secondary metabolites, called interference competition

    The overall effect of all competition based mechanisms and the abiotic environment on disease development is known as “general disease suppression” and is the sum of all factors that reduce disease. The aim of this thesis was to study different aspects of general disease suppression, in order to get more insight into the interplay between microbial communities, pathogen dynamics, and substrate availability in different agricultural soils.

    The first objective was to study the role of microbial volatile organic compounds in natural disease suppression in agricultural soils. In chapter 2 a series of simultaneous experiments were performed on a agricultural soil that received different management practises. We showed a strong correlation between root infection and -biomass production in a bioassay and the suppressive effects of microbial volatiles on the in vitro growth of the pathogen Pythium intermedium. No or weak volatile suppression coincided with significant lower root biomass and a higher disease index, whereas a strong volatile suppression related to high biomass and a low disease index. Furthermore, the composition of the original soil bacterial community showed a drastic shift due to the legacy effects of management practices, coinciding with the loss of volatile suppression. By comparing the emission profiles and the bacterial community composition of the differently managed soils, candidate inhibitory compounds and volatile producing bacterial groups could be identified. Altogether these results indicate that volatile organic compounds can have an important role in general disease suppression.

    To follow up on volatile suppression chapter 3 investigates the influence of soil-related (abiotic and biotic) variables on volatile mediated in vitro growth inhibition of different plant pathogens via an extensive soil survey including 50 Dutch arable agricultural fields. The volatile mediated suppression of three phylogenetic different soil borne pathogens (Rhizoctonia solani, Fusarium oxysporum and Pythium intermedium) was linked to a wide range of soil-related variables with univariate and multivariate regression models. The overall suppression of different pathogens was linked to microbial activity and organic substrates. However, different pathogens showed different sensitivity to volatile suppression. Furthermore, the soil-related factors corresponding to volatile mediated suppression were pathogen specific. In total, the results described in this chapter show that part of volatile suppression for a particular pathogen is based on general microbial activity, but our data shows as well that the individual response is pathogen specific.

    Chapter 4 explores the reservoir of potential plant pathogens harboring agricultural soils before the start of the growth season, together with the environmental drivers of this pool of pathogens. By investigating the pathogenic seedbank in relation to its environment we assessed which soil-related variables could explain differences among site pathogen community composition. Pathogens differing in phylogeny or mode of infection were related to different soil variables. For example the among-site differences in the presence of oomycetes could not be related to their environmental context. On the other hand the variation in root and shoot fungal pathogen community composition was linked to soil physico-chemical properties and non-pathogen microbial community composition, with potentially a significant role of litter saprophytes therein.

    As the presence of pathogen propagules in soil is not necessarily related to disease incidence, chapter 5 investigates the dynamics of root pathogens in the presence of a root in a model rhizosphere. We developed a qPCR based assay to test the growth response of a pathogen (Pythium intermedium) to the presence of root exudates over time. This exposure to root exudates showed soil specific pathogen dynamics. This finding may indicate that in situ (microbial) processes can successfully prevent pathogen development in some of the soils but not in others. Thus this method has the potential to provide an alternative way to assess the susceptibility of a soil to certain soil-borne diseases.

    The results of this thesis gave new insights into different aspects of disease suppression in agricultural soils which could serve as a fundament to develop environmentally-friendly control methods based on natural occurring ecological processes. Ideas for the implementation of this study and future research are discussed in chapter 6.

    Virus : buitenland kijkt al lang mee
    Dwarswaard, A. ; Kock, M. de - \ 2014
    BloembollenVisie 2014 (2014)313. - ISSN 1571-5558 - p. 75 - 75.
    bloembollen - landbouwkundig onderzoek - effecten - virussen - plantenziekteverwekkers - gewasbescherming - plantenveredeling - resistentie van variëteiten - ornamental bulbs - agricultural research - effects - viruses - plant pathogens - plant protection - plant breeding - varietal resistance
    De afgelopen eeuw is er veel onderzoek gedaan aan bloembollen. Praktische problemen werden opgelost. Nu de sector op een keerpunt staat waar het gaat om de collectieve financiering van onderzoek, is het goed om nog eens na te gaan wat het onderzoek de praktijk heeft gebracht. in deze serie staat die vraag centraal. Dit keer: onderzoek aan virussen.
    Detectie van plantenpathogenen en –aantasters
    Bonants, P.J.M. ; Lee, T.A.J. van der; Vlugt, R.A.A. van der; Dullemans, A.M. ; Verbeek, M. ; Ebskamp, M. ; Bruinsma, M. ; Kooman, M. ; Haan, E. de; Kock, M. de; Miglino, R. ; Kox, L. - \ 2014
    Gewasbescherming 45 (2014)5. - ISSN 0166-6495 - p. 157 - 158.
    conferenties - plantenziekteverwekkers - kwaliteitscontroles - inspectie - diagnostische technieken - samenwerking - detectie - gewasbescherming - conferences - plant pathogens - quality controls - inspection - diagnostic techniques - cooperation - detection - plant protection
    Nederland speelt internationaal een belangrijke rol in productie en handel van plantaardig materiaal. Hierin zijn vaak ongewenste veroorzakers van quarantaine- (Q) en kwaliteitsziekten (K) en -plagen een storende factor. Om de (fytosanitaire) kwaliteit van verhandeld (met name ook geëxporteerd) plantmateriaal ook in de toekomst te kunnen blijven garanderen, en eigen teelten te vrijwaren van ongewenste organismen is het van groot belang vroegtijdig deze plantenpathogenen te kunnen detecteren en te identificeren. Hiervoor zijn gevalideerde onderzoekmethoden noodzakelijk, die geschikt zijn voor routinematig gebruik door het bedrijfsleven, de keuringsdiensten en de NVWA. De hier voorgestelde intensieve samenwerking tussen kennisinstellingen, overheid en keuringslaboratoria is noodzakelijk om de agrarische sector toekomstbestendig te houden. Daarnaast dient naast aandacht voor nieuwe ontwikkelingen ook aandacht besteedt te worden aan kwaliteitsborging en efficiëntie verbetering van bestaande technieken zodat de taken van de keuringsdiensten beter uitgevoerd kunnen worden.
    Detection and Diagnostics of Plant Pathogens
    Gullino, M.L. ; Bonants, P.J.M. - \ 2014
    Dordrecht, The Netherlands : Springer (Plant pathology in the 21st Century volume 5) - ISBN 9789401790192 - 200
    plantenziekteverwekkers - detectie - diagnostiek - plantenziekten - diagnostische technieken - plant pathogens - detection - diagnostics - plant diseases - diagnostic techniques
    This book is part of the Plant Pathology in the 21st Century Series, started in the occasion of the IX International Congress of Plant Pathology, Torino, 2008. In conjunction with the Xth International Congress of Plant Pathology, held in Beijing in August 2013. Although deriving from a Congress, the book will not have the format of traditional Proceedings, but will be organized as a resource book. It will be based on invited lectures presented at the Congress as well as by other chapters selected by the editors among offered papers. This book will cover a topic very important in the field of plant pathology, dealing with detection and diagnostics. This field of research is continuously moving forwards, due to innovation in techniques. The application of new detection and diagnostic technologies are relevant to many applied fields in agriculture. The different chapters will provide a very complete figure of the topic, from general and basic aspects to practical aspects. Contents: 1. New developments in identification and quantification of airborne inoculum 2. siRNA deep sequencing and assembly: piecing together viral infections 3. Use of airborne inoculum detection for disease management decisions 4. Proximal sensing of plant diseases Case studies and special applications 5. Diagnostic Challenges for the Detection of Emerging Pathogens: A Case Study Involving the Incursion of Pseudomonas syringae pv. actinidiae in New Zealand 6. Detection of Human Pathogens on Plants - 7. Plant Disease Diagnostics For Forensic Applications III. Role of diagnostics in plant disease management 8. Results of the EU project QBOL, focusing on DNA barcoding of quarantine organisms, added to an international database (Q-bank) on identification of plant quarantine pathogens and relatives 9. On-site testing - moving decision making from the lab to the field 10. Virtual Diagnostic Networks: A platform for collaborative diagnostics 11. Development and implementation of rapid and specific detection techniques for seed-borne pathogens of leafy vegetable crops. 12. Diagnosis of plant pathogens and implications for plant health regulation: the European Food Safety Authority perspective
    PPO zoekt naar mogelijkheden aanpak Burkholderia
    Dwarswaard, A. ; Dam, M.F.N. van - \ 2014
    BloembollenVisie 2014 (2014)302. - ISSN 1571-5558 - p. 16 - 16.
    gladiolus - bloembollen - bacterieziekten - burkholderia - plantenziekteverwekkers - plantenziekteverwekkende bacteriën - cultivars - teeltsystemen - landbouwkundig onderzoek - gladiolus - ornamental bulbs - bacterial diseases - burkholderia - plant pathogens - plant pathogenic bacteria - cultivars - cropping systems - agricultural research
    In de bloemen- en knollenteelt van gladiool komt de afgelopen decennia met enige regelmaat de bacterieziekte Burkholderia voor. Vorig jaar startte PPO met een onderzoek naar de mogelijkheden om deze ziekte aan te pakken. Een tussenstand.
    Oligotrophic bacteria and root disease suppression in organically managed soils
    Senechkin, I.V. - \ 2013
    Wageningen University. Promotor(en): Ariena van Bruggen, co-promotor(en): Leo van Overbeek. - Wageningen : Wageningen University - ISBN 9789461738035 - 141
    bodempathogenen - plantenziekteverwekkers - thanatephorus cucumeris - fusarium oxysporum - bodembacteriën - bodembeheer - biologische landbouw - linum usitatissimum - vlas - modellen - bodemweerbaarheid - soilborne pathogens - plant pathogens - thanatephorus cucumeris - fusarium oxysporum - soil bacteria - soil management - organic farming - linum usitatissimum - flax - models - soil suppressiveness

    The objective of this thesis was to obtain a better understanding of soil health in terms of microbial and chemical characteristics as well as suppression of soil borne plant pathogens. Organic soils were chosen as an appropriate model for studying soil health. Four different organic amendments were applied on two experimental fields with different crop history, providing a diverse range of soil quality levels. Many soil microbial variables were measured, including copiotrophic and oligotrophic bacterial populations, the abundance and diversity of Eubacteria, Alphaproteobacteria, Pseudomonas and fungal communities, as well as several microbial genes involved in nitrogen cycling. Plant disease suppressiveness was used as a quantitative integrative parameter reflecting the health status of soils. Rhizoctonia solani on beet and Fusarium oxysporum on flax were selected as pathosystems; areas under disease progress curves were measured in bioassays with differentially amended field soils and were related to soil parameters. Combined rather than single amendments enhanced Fusarium suppression, but Rhizoctonia suppression was more related to crop history than organic amendments. No universal correlations were found between disease suppression and microbial and chemical parameters, although pH and organic matter affected microbial communities and Fusarium wilt. A significant relation between ammonia oxidizing bacteria and disease suppression was observed for both pathogens; this relation was likely indirect via nitrogen availability and pH. No direct relationship was found between quantities of N cycling genes and disease suppression. A specific emphasis was put on the potential role of oligotrophic bacteria in soil health and disease suppression. Bacteria isolated on low carbon medium (10 mg C/L) were repeatedly transferred onto this medium to select true oligotrophic bacteria. Most isolates could grow on both low carbon and higher carbon (1000 mg C/L) media and belonged to Streptomyces, Rhizobium, Bradyrhizobium and Mesorhizobium. A new oligotrophic isolate was identified as Collimonas sp. IS343 and its interaction with R. solani was studied. This strain was better adapted to oligotrophic conditions than a copiotrophic Collimonas reference strain and was more effective in controlling R. solani. This thesis provided a better understanding of some aspects of soil health and emphasized the role of oligotrophic bacteria, a poorly understood but very important group of soil inhabitants.

    Aantonen stengelaaltje in uitschot: wel in lab, niet in praktijk
    Dees, R.H.L. ; Doorn, J. van; Vreeburg, P.J.M. ; Sibbel, L. - \ 2013
    BloembollenVisie 2013 (2013)274. - ISSN 1571-5558 - p. 22 - 23.
    tulpen - bollen - ditylenchus dipsaci - plantenziekteverwekkers - tests - besmetting - landbouwkundig onderzoek - tulips - bulbs - ditylenchus dipsaci - plant pathogens - tests - contamination - agricultural research
    Stengelaaltjes vormen een sluipend probleem in de teelt van tulpen. Vaak wordt de teler toch door een aantasting verrast. De vraag aan PPO was om een financieel acceptabele en praktisch uitvoerbare test te ontwikkelen om al snel te weten of een partij tulpen is besmet met het tulpenstengelaaltje.
    'Intraspecific pathogen variation' Verslag KNPV/Plantum/EPS-eendagsconferentie : Wageningen, 22 januari 2013
    Folkertsma, R.T. ; Goverse, A. ; Posthuma, E. ; Gilijamse, T. - \ 2013
    Gewasbescherming 44 (2013)2. - ISSN 0166-6495 - p. 32 - 34.
    plantenziekteverwekkers - genetische variatie - genotypische variatie - dna-sequencing - moleculaire technieken - moleculaire genetica - plantenziekten - conferenties - plant pathogens - genetic variation - genetic variance - dna sequencing - molecular techniques - molecular genetics - plant diseases - conferences
    Dinsdag 22 januari 2013 werd in Wageningen een eendagsconferentie gehouden getiteld ‘Intraspecific pathogen variation - implications and opportunities’. Deze conferentie werd georganiseerd naar aanleiding van discussies over het werken met intraspecifieke variatie voor diagnostiek en veredeling binnen de Nematodenwerkgroep van de KNPV en de Isolaten-beheerwerkgroep van Plantum. Het doel van de bijeenkomst was a.) onderzoekers uit de private en de publieke sector samenbrengen om recente ontwikkelingen te bespreken in fundamentele en toegepaste aspecten van het werken met intraspecifieke variatie, en b.) het stimuleren van uitwisselen van ideeën binnen en tussen beide groepen, voor mogelijke vervolginitiatieven.
    Aanpak van overmatige wortelgroei in vruchtgroentegewassen
    Ludeking, D.J.W. ; Hamelink, R. ; Wubben, J.P. ; Wubben, J. ; Schenk, M.F. - \ 2013
    Bleiswijk : Wageningen UR Glastuinbouw (Rapporten WUR GTB 1244) - 34
    kasgewassen - vruchtgroenten - rhizobium rhizogenes - worteloppervlak - plantenziekteverwekkers - substraten - drainagewater - tests - bestrijdingsmethoden - glastuinbouw - greenhouse crops - fruit vegetables - rhizobium rhizogenes - rhizoplane - plant pathogens - substrates - drainage water - tests - control methods - greenhouse horticulture
    Bij de teelt van komkommer, tomaat en aubergine in uiteen lopende glastuinbouwgebieden in Nederland worden planten waargenomen in de teelt waarbij het wortelgestel een overmatige productie aan wortels laat zien. Het fenomeen dat daarom ook ‘overmatige wortelgroei’ wordt genoemd, uit zich door het ontstaan van veel extra wortels aan het grond- of steenwoloppervlak. De oorzaak van deze overmatige wortelgroei is de bacterie Agrobacterium rhizogenes. Deze bacterie wordt ook wel Agrobacterium radiobacter genoemd . In dit experiment zijn verschillende methoden of middelen onderzocht die de aanwezigheid en infectie van wortels met de Ri-plasmide afkomstig uit Agrobacterium rhizogenes in een kas kunnen voorkomen of verspreiding ervan kunnen inperken. De uitgevoerde proeven hebben als doel: Het ontwikkelen en testen van effectieve maatregelen die de aanwezigheid en verspreiding van overmatige wortelgroei in vruchtgroentegewassen kunnen beperken.
    Minder overmatige wortels door grotere weerbaarheid planten en substraat (interview met Marta Streminska, André van der Wurff en Daniël Ludeking)
    Arkesteijn, M. ; Streminska, M.A. ; Wurff, A.W.G. van der; Ludeking, D.J.W. - \ 2013
    Onder Glas 10 (2013)2. - p. 40 - 41.
    glastuinbouw - vruchtgroenten - potplanten - worteloppervlak - rhizobium rhizogenes - substraten - bestrijdingsmethoden - plantenziekteverwekkers - tests - groenten - greenhouse horticulture - fruit vegetables - pot plants - rhizoplane - rhizobium rhizogenes - substrates - control methods - plant pathogens - tests - vegetables
    Overmatige wortelgroei is een nachtmerrie voor veel telers van tomaat, komkommer en aubergine. Veroorzaker is Agrobacterium rhizogenes. Na besmetting gaan de wortels van zieke planten overmatig groeien. Het aantal aangetaste planten varieert van enkele tot 100%. De symptomen zorgen voor stressvolle omstandigheden en voor een teruglopende productie.
    Onderzoek rol insecten bij overdracht komkommerbontvirus
    Stijger, I. ; Hamelink, R. - \ 2013
    Onder Glas 10 (2013)2. - p. 35 - 35.
    plantenziekteverwekkers - plantenvirussen - mijten - komkommerbontvirus - overdracht - insecten - gewasbescherming - glastuinbouw - groenten - plant pathogens - plant viruses - mites - cucumber green mottle mosaic virus - transfer - insects - plant protection - greenhouse horticulture - vegetables
    Het komkommerbontvirus is een zeer persistent virus. Bekend is dat dit virus via mechanische overdracht en zaad kan worden verspreid. Mensen kunnen via de vele gewashandelingen het virus van plant naar plant overdragen.
    Weerbaarheid, ook tegen plantenvirussen
    Kock, M.J.D. de; Stijger, I. - \ 2013
    BloembollenVisie 2013 (2013)262. - ISSN 1571-5558 - p. 18 - 19.
    plantenziekteverwekkers - plantenvirussen - infectiebestrijding - bedrijfshygiëne - bestrijdingsmethoden - landbouwkundig onderzoek - planten - plant pathogens - plant viruses - infection control - industrial hygiene - control methods - agricultural research - plants
    Op een nieuwe manier naar de aanpak van virussen kijken. Dat doen de onderzoekers Maarten de Kock en Ineke Stijger. In dit artikel gaan ze na welke mogelijkheden er liggen op het gebied van weerbaarheid om plantenvirussen aan te pakken.
    Tools voor identificatie en detectie van quarantaine plantenpathogenen (Parallelle sessie A-II: Risicomanagement - Inzicht en Ingrijpen: waar, wanneer en hoe?)
    Bonants, P.J.M. - \ 2011
    In: KNPV-voorjaarsvergadering: ‘Plantgezondheid grenzeloos! Fytosanitair nader belicht’, Wageningen, 8 juni 2011. - Wageningen : KNPV - p. 125 - 125.
    plantenziekten - detectie - moleculaire detectie - plantenziekteverwekkers - quarantaine organismen - plant diseases - detection - molecular detection - plant pathogens - quarantine organisms
    Samenvatting van de sessie 'tools voor identificatie en detectie van quarantaine plantenpathogenen' op de KNPV-voorjaarsvergadering, 8 juni 2011.
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