Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Novel COX-2 products of n-3 polyunsaturated fatty acid-ethanolamine-conjugates identified in RAW264.7 macrophages
    Bus, Ian de; Zuilhof, Han ; Witkamp, Renger ; Balvers, Michiel ; Albada, Bauke - \ 2019
    Journal of Lipid Research 60 (2019)11. - ISSN 0022-2275 - p. 1829 - 1840.
    cyclooxygenase - cyclooxygenase 2 - fatty acid amides - fatty acid oxidation - high-performance liquid chromatography - inflammation - mass spectrometry - prostaglandins

    Cyclooxygenase 2 (COX-2) plays a key role in the regulation of inflammation by catalyzing the oxygenation of PUFAs to prostaglandins (PGs) and hydroperoxides. Next to this, COX-2 can metabolize neutral lipids, including endocannabinoid-like esters and amides. We developed an LC-HRMS-based human recombinant (h)COX-2 screening assay to examine its ability to also convert n-3 PUFA-derived N-acylethanolamines. Our assay yields known hCOX-2-derived products from established PUFAs and anandamide. Subsequently, we proved that eicosapentaenoylethanolamide (EPEA), the N-acylethanolamine derivative of EPA, is converted into PGE3-ethanolamide (PGE3-EA), and into 11-, 14-, and 18-hydroxyeicosapentaenoyl-EA (11-, 14-, and 18-HEPE-EA, respectively). Interestingly, we demonstrated that docosahexaenoylethanolamide (DHEA) is converted by hCOX-2 into the previously unknown metabolites, 13- and 16-hydroxy-DHEA (13- and 16-HDHEA, respectively). These products were also produced by lipopolysaccharide-stimulated RAW267.4 macrophages incubated with DHEA. No oxygenated DHEA metabolites were detected when the selective COX-2 inhibitor, celecoxib, was added to the cells, further underlining the role of COX-2 in the formation of the novel hydroxylated products. This work demonstrates for the first time that DHEA and EPEA are converted by COX-2 into previously unknown hydroxylated metabolites and invites future studies toward the biological effects of these metabolites.

    Het gebruik van Prostaglandine-FZalpha na werpen
    Loo, D. van de - \ 1995
    Praktijkonderzoek varkenshouderij 9 (1995)6. - ISSN 1382-0346 - p. 24 - 24.
    geboorte - vruchtbaarheid - oestrus - prostacycline - prostaglandinen - zeugen - birth - fertility - oestrus - prostacyclin - prostaglandins - sows
    Prostaglandine-FZalpha, zoals Dinolytice, Ilirene, Planatee en Prosolvine wordt in de Nederlandse varkenshouderij toegepast om geboorten te synchroniseren. Daarnaast zou het gebruik van Prostaglandine-F2alpha na het werpen de baarmoeder sneller en beter schoon krijgen. Dit zou een positief effect hebben op de vruchtbaarheid bij de volgende worp.
    Bedrijfservaringen met het opwekken van geboorten
    Loo, D. van de - \ 1995
    Praktijkonderzoek varkenshouderij 9 (1995)6. - ISSN 1382-0346 - p. 8 - 9.
    dierhouderij - geboorte - productiviteit - rentabiliteit - prostacycline - prostaglandinen - animal husbandry - birth - productivity - profitability - prostacyclin - prostaglandins
    Prostaglandine-F2alpha (verkocht onder de merknamen Dinolytice, Ilirene, Planatee, Prosolvine etcetera) wordt op sommige varkensbedrijven toegepast om geboorten te synchroniseren. Op het Varkensproefbedrijf te Sterksel zijn bedrijfservaringen opgedaanmet Dinolytice. Dit blijkt goed toepasbaar om de geboorte op te wekken.
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