Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Spatiotemporal proliferation of human stromal cells adjusts to nutrient availability and leads to stanniocalcin-1 expression in vitro and in vivo
    Higuera, G.A. ; Fernandes, H. ; Spitters, T.W.G.M. ; Boxtel, A.J.B. van - \ 2015
    Biomaterials 61 (2015). - ISSN 0142-9612 - p. 190 - 202.
    mesenchymal stem-cells - tissue engineering scaffolds - bone-marrow - mechanical compression - mathematical-model - progenitor cells - up-regulation - shear-stress - gradients - oxygen
    Cells and tissues are intrinsically adapted to molecular gradients and use them to maintain or change their activity. The effect of such gradients is particularly important for cell populations that have an intrinsic capacity to differentiate into multiple cell lineages, such as bone marrow derived mesenchymal stromal cells (MSCs). Our results showed that nutrient gradients prompt the spatiotemporal organization of MSCs in 3D culture. Cells adapted to their 3D environment without significant cell death or cell differentiation. Kinetics data and whole-genome gene expression analysis suggest that a low proliferation activity phenotype predominates in stromal cells cultured in 3D, likely due to increasing nutrient limitation. These differences implied that despite similar surface areas available for cell attachment, higher cell concentrations in 3D reduced MSCs proliferation, while activating hypoxia related-pathways. To further understand the in vivo effects of both proliferation and cell concentrations, we increased cell concentrations in small (1.8 ml) implantable wells. We found that MSCs accumulation and conditioning by nutrient competition in small volumes leads to an ideal threshold of cell-concentration for the induction of blood vessel formation, possibly signaled by the hypoxia-related stanniocalcin-1 gene.
    The physics of tissue formation with mesenchymal stem cells
    Higuera, G.A. ; Boxtel, A.J.B. van; Blitterswijk, C.A. van; Moroni, L. - \ 2012
    Trends in Biotechnology 30 (2012)11. - ISSN 0167-7799 - p. 583 - 590.
    bone-marrow - chondrogenic differentiation - mechanical-properties - bioreactor system - shear-stress - iron-oxide - in-vitro - expansion - proliferation - micro
    Cells react to various forms of physical phenomena that promote and maintain the formation of tissues. The best example of this are cells of musculoskeletal origin, such as mesenchymal stem cells (MSCs), which consistently proliferate or differentiate under cues from hydrostatic pressure, diffusive mass transport, shear stress, surface chemistry, mechanotransduction, and molecular kinetics. To date, no other cell type shows greater receptiveness to macroscopic and microscopic cues, highlighting the acute sensitivity of MSCs and the importance of physical principles in tissue homeostasis. In this review, we describe the literature that has shown how physical phenomena govern MSCs biology and provide insight into the mechanisms and strategies that can spur new biotechnological applications with tissue biology.
    Discharge estimation from H-ADCP measurements in a tidal river subject to sidewall effects and a mobile bed
    Sassi, M.G. ; Hoitink, A.J.F. ; Vermeulen, B. ; Hidayat, H. - \ 2011
    Water Resources Research 47 (2011). - ISSN 0043-1397 - 14 p.
    open-channel flows - shear-stress - sediment transport - secondary currents - velocity - roughness - sand
    Horizontal acoustic Doppler current profilers (H-ADCPs) can be employed to estimate river discharge based on water level measurements and flow velocity array data across a river transect. A new method is presented that accounts for the dip in velocity near the water surface, which is caused by sidewall effects that decrease with the width to depth ratio of a channel. A boundary layer model is introduced to convert single-depth velocity data from the H-ADCP to specific discharge. The parameters of the model include the local roughness length and a dip correction factor, which accounts for the sidewall effects. A regression model is employed to translate specific discharge to total discharge. The method was tested in the River Mahakam, representing a large river of complex bathymetry, where part of the flow is intrinsically three-dimensional and discharge rates exceed 8000 m3 s-1. Results from five moving boat ADCP campaigns covering separate semidiurnal tidal cycles are presented, three of which are used for calibration purposes, whereas the remaining two served for validation of the method. The dip correction factor showed a significant correlation with distance to the wall and bears a strong relation to secondary currents. The sidewall effects appeared to remain relatively constant throughout the tidal cycles under study. Bed roughness length is estimated at periods of maximum velocity, showing more variation at subtidal than at intratidal time scales. Intratidal variations were particularly obvious during bidirectional flow conditions, which occurred only during conditions of low river discharge. The new method was shown to outperform the widely used index velocity method by systematically reducing the relative error in the discharge estimates
    Forces involved in bacterial adhesion to hydrophilic and hydrophobic surfaces
    Boks, N.P. ; Norde, W. ; Meil, H.C. ; Busscher, H.J. - \ 2008
    Microbiology 154 (2008). - ISSN 1350-0872 - p. 3122 - 3133.
    escherichia-coli - staphylococcus-epidermidis - microbial adhesion - optical tweezers - pseudomonas-fluorescens - coated surfaces - parallel-plate - shear-stress - flow chamber - deposition
    Using a parallel-plate flow chamber, the hydrodynamic shear forces to prevent bacterial adhesion (F-prev) and to detach adhering bacteria (F-det) were evaluated for hydrophilic glass, hydrophobic, dimethyldichlorosilane (DDS)-coated glass and six different bacterial strains, in order to test the following three hypotheses. 1. A strong hydrodynamic shear force to prevent adhesion relates to a strong hydrodynamic shear force to detach an adhering organism. 2. A weak hydrodynamic shear force to detach adhering bacteria implies that more bacteria will be stimulated to detach by passing an air-liquid interface (an air bubble) through the flow chamber. 3. DLVO (Derjaguin, Landau, Verwey, Overbeek) interactions determine the characteristic hydrodynamic shear forces to prevent adhesion and to detach adhering micro-organisms as well as the detachment induced by a passing air-liquid interface. F-prev varied from 0.03 to 0.70 pN, while F-det varied from 0.31 to over 19.64 pN, suggesting that after initial contact, strengthening of the bond occurs. Generally, it was more difficult to detach bacteria from DIDS-coated glass than from hydrophilic glass, which was confirmed by air bubble detachment studies. Calculated attractive forces based on the DLVO theory (F-DLVO) towards the secondary interaction minimum were higher on glass than on DIDS-coated glass. In general, all three hypotheses had to be rejected, showing that it is important to distinguish between forces acting parallel (hydrodynamic shear) and perpendicular (DLVO, air-liquid interface passages) to the substratum surface.
    A biofilm model for flowing systems in the food industry
    Asselt-den Aantrekker, E.D. van; Vernooij, W.W. ; Reij, M.W. ; Zwietering, M.H. ; Beumer, R.R. ; Schothorst, M. van; Boom, R.M. - \ 2003
    Journal of Food Protection 66 (2003)8. - ISSN 0362-028X - p. 1432 - 1438.
    bacterial biofilms - meat surfaces - shear-stress - detachment - attachment - kinetics
    When bacteria attach to the walls of pipelines, they can form biofilms, which can cause the recontamination of food products. In order to quantify such recontamination, a one-dimensional biofilm model was developed taking into account adsorption, desorption, and the growth of cells. The model consisted of two mass balances describing increases in biofilm formation at the wall and the accumulation of cells in the liquid phase. The necessary parameters for the model were obtained in laboratory biofilm experiments. These experiments involved a flowing system and the use of Staphylococcus aureus as a model pathogen and silicon tubing as a testing material. S. aureus was inoculated into the system for 2 h, and then the system was changed to a sterile medium. Both biofilm formation and the release of cells into the flowing liquid were measured until steady-state conditions were reached (for up to 9 days). The experiments were performed in duplicate for different flow conditions (i.e., for Reynolds numbers of 3.2, 32, and 170). It was shown that at higher Reynolds numbers, the biofilm developed faster, probably owing to an increase in the transfer of nutrients to the surface. The proposed biofilm model was capable of describing the data obtained for the three different flow conditions with the use of the specific growth rate in the biofilm and the desorption coefficient as fit parameters. The specific growth rates were 0.16, 0.27, and 0.49 h(-1) for Reynolds numbers of 3.2, 32, and 170, respectively, and the desorption coefficients were about 1% of these values.
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