Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Reproductive biology and induced sterility as determinants for genetic control of mosquitoes with the Sterile Insect Technique
    Helinski, M. - \ 2008
    Wageningen University. Promotor(en): Marcel Dicke, co-promotor(en): B.G.J. Knols. - S.l. : S.n. - ISBN 9789085049555 - 203
    steriele insecten techniek - genetische gewasbescherming - sterilisatie - ioniserende straling - stabiele isotopen - ziekten overgebracht door muskieten - malaria - anopheles arabiensis - paarconcurrentie - sudan - sterile insect release - genetic control - sterilization - ionizing radiation - stable isotopes - mosquito-borne diseases - malaria - anopheles arabiensis - mating competitiveness - sudan
    Malaria remains an important health issue in sub-Saharan Africa, and new methods to reduce the disease are needed. In this thesis, the use of the Sterile Insect Technique (SIT) against the African malaria vector Anopheles arabiensis was explored. The SIT relies on the releases of large numbers of sterilised males. If the sterile males are successful in competing for mates and the females use their sperm for fertilisation, the wild population is reduced. Ultimately, this can lead to a reduction in disease incidence. Sterilisation of the sperm cells occurs by ionising radiation, resulting in the death of the developing embryo after fertilisation. Somatic cells are also damaged, which can lead to a reduced mating competitiveness of the males.
    In this thesis I investigated 1) the relationship between dose and induced sterility for pupal or adult stage irradiation, 2) sperm quantity and sperm length polymorphism and the influence of irradiation, 3) the use of stable isotopes to determine mating in mosquitoes, 4) the incidence of multiple mating in relation to irradiation, 5) the fitness of irradiated males in terms of survival and mating competitiveness, and 6) the use of a field cage for mating studies, and the small-scale feasibility of the SIT in Sudan.
    Results showed that even though the dose-response curves between dose and induced sterility were largely similar for pupae and adults, irradiation of adults resulted in a better competitiveness compared to pupal irradiation. A negative relationship between dose and competitiveness was observed for pupal stage irradiation. In addition, radiation during the pupal stage affected the number of spermatozoa in the testes and the distribution of sperm lengths, but no impact on the incidence of multiple mating could be observed. Stable isotopes were used successfully to determine paternity in mating. Mating competitiveness of males irradiated as pupae could be improved by a three-fold increase in their number compared to un-irradiated males, but only for the partially-sterilising dose. The small-scale irradiation and transportation of insects in Sudan was feasible, and the preparation of the field cage for experiments successful.
    It is concluded that from a biological viewpoint the irradiation of adults would be recommended; however, the feasibility of adult irradiation on a large scale is questionable. The next steps would be to scale up irradiation procedures to accommodate much larger numbers of insects, and to determine male competitiveness in the semi-field system in Sudan. However, many other factors including mass rearing, sexing, and release methodology, are of importance for an SIT programme and only when all components are in place can the true feasibility of the SIT in Sudan be determined.

    Radiation induced sterility to control tsetse flies : the effect of ionising radiation and hybridisation on tsetse biology and the use of the sterile insect technique in integrated tsetse control
    Vreysen, M.J.B. - \ 1995
    Agricultural University. Promotor(en): J.C. van Lenteren; W. Takken. - S.l. : Vreysen - ISBN 9789054854432 - 282
    sterilisatie - insecten - steriele insecten techniek - genetische gewasbescherming - vectorbestrijding - infectieziekten - anthomyiidae - muscidae - drosophilidae - calliphoridae - agromyzidae - tachinidae - glossinidae - tephritidae - glossina - schizophora - sterilization - insects - sterile insect release - genetic control - vector control - infectious diseases - anthomyiidae - muscidae - drosophilidae - calliphoridae - agromyzidae - tachinidae - glossinidae - tephritidae - glossina - schizophora

    The induction of dominant lethal mutations by exposing tsetse flies as pupae or adult insects to ionising radiation and the use of hybrid sterility resulting from crosses of closely related tsetse species or subspecies, are potential methods of genetic control of tsetse flies. In this thesis the effects of radiation and hybridisation on the reproductive biology and fitness of several species of tsetse flies has been examined. In addition, aspects of field releases of sterile insects have also been examined.

    De gevleugelde missie van Thijs Loosjes.
    Beekman, W. - \ 1994
    Ekoland 14 (1994)12. - ISSN 0926-9142 - p. 20 - 21.
    agromyzidae - allium cepa - anthomyiidae - nuttige insecten - biologische bestrijding - calliphoridae - drosophilidae - genetische gewasbescherming - glossinidae - insecten - muscidae - uien - steriele insecten techniek - sterilisatie - tachinidae - tephritidae - delia antiqua - schizophora - agromyzidae - allium cepa - anthomyiidae - beneficial insects - biological control - calliphoridae - drosophilidae - genetic control - glossinidae - insects - muscidae - onions - sterile insect release - sterilization - tachinidae - tephritidae - delia antiqua - schizophora
    Interview with Thijs Loosjes, who runs a firm for biological control in agriculture. The firm is specialized in biocontrol of the onion fly by means of the sterile male technique
    Translocations and sex ratio distortion in the onion fly, Hylemya antiqua (Meigen), and their relevance to genetic control
    Vosselman, L. - \ 1980
    Landbouwhogeschool Wageningen. Promotor(en): J. Sybenga. - Wageningen : Vosselman - 117
    agromyzidae - anthomyiidae - calliphoridae - chromosoomtranslocatie - drosophilidae - genetische gewasbescherming - genetica - glossinidae - insecten - muscidae - geslacht (sex) - geslachtsbepaling - steriele insecten techniek - sterilisatie - tachinidae - tephritidae - stofverplaatsing - schizophora - agromyzidae - anthomyiidae - calliphoridae - chromosome translocation - drosophilidae - genetic control - genetics - glossinidae - insects - muscidae - sex - sex determination - sterile insect release - sterilization - tachinidae - tephritidae - translocation - schizophora
    Experiments on sex determination of the onion fly, Hylemya antiqua (Meigen), are reported in the first two chapters. The main purpose was to establish if the sex ratio distortion observed in certain crosses, was based on a mechanism which could be used for genetic purposes. Two types of males, XY 1 and XXY 2 , were found and the aberrant sex ratios appeared only to occur in progenies of XXY 2 (and some XXY 2 Y 2 ) males. A numerical variation for Y 2 was frequently observed in embryos, larval ganglion cells and especially in testes, which is caused by numerical non-disjunction in somatic cells. The sex ratio distortion is attributed to such a numerical variation of Y 2 between primordial germ cells. Some progenies with a highly distorted sex ratio in male direction descended probably from XXY 2 Y 2 males. Since an effective selection of XXY 2 Y 2 males is not possible, this type of sex ratio distortion is not suited for application in a genetic control method. Other subjects treated in chapters 1 and 2 are gynandromorphism, a polymorphism with respect to the length of the X-chromosome and X-polysomy. In the progeny of one cross a chromosome morphologically identical to Y 2 but lacking its holandric inheritance was found. From its inheritance in various crosses it could be concluded that it was nothing more than a (mutated) non-functional Y 2 chromosome.

    The meiotic segregation of a Y 1 -linked translocation T61 is reported in the third chapter. The high percentage of alternate segregation observed in T61-heterozygous males (22 YXY 2) was in good accordance with the high fertility of this translocation (95%). Because of an early separation (or asynapsis) of the centromeric regions of the acrocentric X and Y 2, chain quadrivalents with X and Y 2in terminal position predominated in diakinesis/ prometaphase I stages. In these chain quadrivalents adjacent l is supposed to be unstable since neither X nor Y 2have a neighbouring centromere in trans-position. The very low frequency of adjacent 2 is attributed to a preferential coorientation of homologous centromeres. The suitability of T61 for genetic sexing (preferential killing of females) is discussed.

    Double-translocation heterozygous T14/T61 males (2 62 Y6 26XY 2) were produced by intercrossing T14-homozygous females (2 62 66 26 2XX) and T61-heterozygous males (22 Y66XY 2) These double heterozygotes showed several segregation types but for predominated. The total frequency of duplication/deficiency gametes was 60-65%. Application possibilities for these heterozygotes in genetic control of the onion fly are discussed.

    The meiotic segregation of five different autosomal reciprocal translocations are given in chapter 5. For translocation T14, which has very short interstitial segments, significant differences in segregation between the sexes were found. In males the ratio of alt.: adj.1:adj.2 was about 7:3:0 and in females 8:1:3. It is assumed that the very intimate meiotic pairing in males (without chiasmata) results in relatively very short "Coorientation Determining Distances" (CDD's) between the homologous centromeres. Due to these short CDD's a preferential coorientation between homologous centromeres is expected favouring alternate and adjacent l segregation. On the contrary, in females chiasmata act very probably as reference points for coorientation. Due to variable positions of the centromeres, in T14-heterozygous females less pronounced differences in CDD's between homologous and non-homologous are expected as in males. This is suggested to be the reason that in females a coorientation between non-homologous centromeres can occur resulting in a certain frequency of adjacent 2. The very low frequencies or absence of adjacent 2 in the other four translocations is attributed to their longer interstitial segments.

    For translocation T14 a pure breeding stock of homozygotes could be obtained using egg-hatch percentages and cytology for the recognition of the karyotypes (chapter 6). In cage experiments under laboratory conditions the competitiveness of the translocation homozygotes was tested. Starting from an initial population consisting of equal numbers of translocation homozygotes (TT) and wild type flies (++), the karyotype frequencies were determined for three successive, non-overlapping generations. The results obtained did not suggest substantial differences in fitness between TT and ++. In field cage experiments (chapter 7) a high diapause sensitivity was found for the translocation homozygotes as well as for the laboratory strain of wild type flies (++) used. As a consequence of this high diapause sensitivity the fitness of the homozygotes could not be determined under field conditions.

    In the "General Discussion" different application possibilities of translocations for genetic control of the onion fly are discussed. A summary of unpublished data concerning the egg-hatch and pupal survival of eight threechromosome double-translocation heterozygotes and three more complex translocation heterozygotes is also presented there. The aim of these experiments was to determine if perhaps a double translocation could be isolated meeting the conditions for a stable equilibrium. However, neither of these translocations met these conditions. Firstly, the frequencies of duplication/deficiency gametes were not high enough; secondly, insufficient complementation occurred and, thirdly, the recombination percentages in the differential segments were too high (resulting in undesirable karyotypes). Two of three double translocations tested, appeared to be viable as homozygotes in the adult stage.

    The use of ionizing radiation in agriculture : proceedings of a workshop, 22, 23 and 24 March 1976, Wageningen, The Netherlands
    Stichting ITAL, ; Association Euratom, - \ 1977
    Commission of the European Communities (Commission of the European Communities EUR 5815 en) - ISBN 9789282500576 - 566
    agriculture - applications - effects - food irradiation - food preservation - genetic control - insects - isotopes - mutations - plant breeding - radiation - radioactivity - sterile insect release - sterilization - landbouw - toepassingen - effecten - voedselbestraling - voedselbewaring - genetische gewasbescherming - insecten - isotopen - mutaties - plantenveredeling - straling - radioactiviteit - steriele insecten techniek - sterilisatie
    Aspects of gametogenesis and radiation pathology in th onion fly. Hylemya antigua (Meigen). II. Radiation pathology
    Theunissen, J.A.B.M. - \ 1977
    Wageningen : Veenman (Mededelingen Landbouwhogeschool Wageningen 77-12) - 132
    agromyzidae - anthomyiidae - calliphoridae - drosophilidae - excretiestelsel - genetische gewasbescherming - glossinidae - insecten - muscidae - plantenplagen - voortplantingsorganen - steriele insecten techniek - sterilisatie - tachinidae - tephritidae - urogenitaalsysteem - delia antiqua - schizophora - agromyzidae - anthomyiidae - calliphoridae - drosophilidae - excretory system - genetic control - glossinidae - insects - muscidae - plant pests - reproductive organs - sterile insect release - sterilization - tachinidae - tephritidae - urogenital system - delia antiqua - schizophora
    Ecology and genetic control of the onion fly, Delia antiqua (Meigen)
    Loosjes, M. - \ 1976
    Wageningen : Pudoc - 179
    agromyzidae - allium cepa - diergedrag - anthomyiidae - calliphoridae - drosophilidae - ecologie - genetische gewasbescherming - glossinidae - gewoonten - insecten - muscidae - uien - steriele insecten techniek - sterilisatie - tachinidae - tephritidae - schizophora - agromyzidae - allium cepa - animal behaviour - anthomyiidae - calliphoridae - drosophilidae - ecology - genetic control - glossinidae - habits - insects - muscidae - onions - sterile insect release - sterilization - tachinidae - tephritidae - schizophora
    Aspects of gametogenesis and radiation pathology in the onion fly, Hylemya antiqua (Meigen) : 1. Gametogenesis
    Theunissen, J.A.B.M. - \ 1976
    Landbouwhogeschool Wageningen. Promotor(en): J. de Wilde; L.P.M. Timmermans. - Wageningen : Veenman - 164
    insecten - plantenplagen - anthomyiidae - muscidae - drosophilidae - calliphoridae - agromyzidae - tachinidae - glossinidae - tephritidae - sterilisatie - steriele insecten techniek - genetische gewasbescherming - excretiestelsel - voortplantingsorganen - urogenitaalsysteem - delia antiqua - schizophora - insects - plant pests - anthomyiidae - muscidae - drosophilidae - calliphoridae - agromyzidae - tachinidae - glossinidae - tephritidae - sterilization - sterile insect release - genetic control - excretory system - reproductive organs - urogenital system - delia antiqua - schizophora

    In the scope of a genetic control research project gametogenesis of the onion fly, Hylemya antiqua (Meigen), is studied as a base for investigations on radiation histopathology of the gonads.

    Various cytological, histological, electronmicroscopical and autoradiographical methods, including investigation of living male germinal cells, are used.

    The gross anatomy of the male and female reproductive systems is simple as compared to other insect species.

    In newly hatched larvae the gonads contain on an average 13 germinal cells. Gonads in larvae which are less than 7 days old cannot be distinguished as being male or female. This distinction becomes possible after the apical cell and the apical somatic tissue respectively is formed in the young gonad.

    Spermatogenesis is treated in a number of paragraphs dealing with the description and identification of germinal and somatic cell types, the ontogenetic development of the testis, the dynamics of spermatogenesis and aspects of comparative spermatogenesis.

    A proper identification of the testicular cell types is considered to be imperative to any correct experimental approach of spermatogenesis. Morphological descriptions of the various germinal and somatic cell types are given accordingly. New elements are the observation of spermatogonial cells which show in their nucleus a varying degree of chromatin concentration. A similar phenomenon is observed in somatic cell types. Testicular cavities namely the apical cavities and the central cavity are described as are the somatic central cavity cells and the basal cells in the testis.

    The ontogenetic development shows the structure and function of the testis during the larval, pupal and adult stages of the life cycle. At the end of the larval stage differentiation of primary spermatogonia starts, leading to sperm production which takes place in the late pupa and the young adult. During adulthood the testis function changes from sperm production towards sperm storage.

    Based on counts of the number of cells per cyst of germinal cells it is supposed that various systems of spermatogonial multiplication operate in these populations. Primary spermatogonia may be denoted as being 'predefinitive' which indicates their presumed role in the multiplication processes. The number of definitive spermatogonial divisions may vary according to the acting multiplication system. The speed of formation of different spermatogenic cell types is revealed by incorporation of 3 H-thymidine in the spermatogonia. Development of a primary spermatogonium into sperm cells lasts about 10 days.

    Comparison of mainly morphological features of male germinal cell types in a number of insect species, including H. antiqua, indicates possibilities for comparative research of spermatogenesis in various insect species. This could provide a basis for comparative radiobiological or other experimental investigations on insect spermatogenesis.

    Female germinal cell types and somatic cell types of the ovary are described. The ontogenetic development of the ovary shows ovariole differentiation in the young pupal ovary. In late pupal ovaries egg chamber formation begins its iterative course which is continued during adulthood. Egg chamber formation has been divided into ten stages.

    Spermatogenesis and oogenesis share a number of features, which suggests a certain similarity of the processes involved.

    Chromosal rearrangements in the onion fly Hylemya antiqua (Meigen), induced and isolated for genetic insect control purposes : studies on cytogenetics and fertility, with emphasis on an X-linked translocation
    Heemert, C. van - \ 1975
    Landbouwhogeschool Wageningen. Promotor(en): J. Sybenga. - Wageningen : [s.n.] - 83
    agromyzidae - anthomyiidae - rassen (dieren) - calliphoridae - drosophilidae - genetische gewasbescherming - genetische variatie - glossinidae - overerving - insecten - muscidae - rassen (taxonomisch) - steriele insecten techniek - sterilisatie - tachinidae - tephritidae - schizophora - agromyzidae - anthomyiidae - breeds - calliphoridae - drosophilidae - genetic control - genetic variation - glossinidae - inheritance - insects - muscidae - races - sterile insect release - sterilization - tachinidae - tephritidae - schizophora
    The aim of this investigation was to isolate structural chromosome mutations causing "semi"-sterility which can be used for genetic control of the onion fly Hylemya antiqua (Meigen). For the induction, X-rays or fast neutrons were applied in different doses on males and females. "Semi"-sterile families were screened cytologically for translocations and inversions. It appeared that high doses 0.5 krad of X-rays) tended to yield complex rearrangements which present difficulties in practical programs, in addition to the useful less complicated rearrangements. They also produce more genetic background damage than lower doses (0-5 krad), which still yield acceptable numbers of uncomplicated rearrangements and therefore are advised for a genetic control program. Because of strongly reduced fecundity of irradiated females these are less suitable for induction of rearrangements than males, although older females because of higher fecundity give better results than young females. Fast neutrons on young males were useful and a low dose of 0.25 krad appeared to be as good as 1.0 krad.

    Seventeen different translocations and two pericentric inversions were observed. Most "breakpoints" are located on the longer chromosome arms. Inversion heterozygous males were as fertile as the control which confirms their achiasmate meiosis. Eight translocation stocks were sibcrossed. In five of these, translocation homozygotes were found as larvae and in three out of these five even as adults. The occurrence of duplication/deficiency (from adjacent I orientation) larvae in the five stocks mentioned was remarkable.

    From experiments with an X-linked translocation it was concluded that the acrocentric chromosomes are the sex-chromosomes. The alternate and adjacent I orientation frequencies in translocation heterozygotes (X-linked) were shown to be equal in each sex, while no adjacent II was found. In this translocation meiotic numerical non- disjunction occurred in 18.7% of the tranlocation heterozygous females but in 2.0% of the males. This difference is probably a consequence of the difference in meiotic behaviour between the sexes. A good relationship was found between the degree of chromosomal unbalance and the frequency of late embryonic lethals (brown eggs) which was used as a graduator in the selection for "semi"-sterility.

    Adult fertile translocation trisomics and adult sterile tertiary trisomics were obtained (both sexes) after meiotic numerical non- disjunction. In translocation trisomic males the X-, Y- and translocated (extra) X-chromosome were shown to disjoin at random. In females the two normal X-chromosomes almost (95%) preferentially disjoin, while the translocated X-chromosome goes to either one of the poles. Primary trisomic males (XXY) and females (XXX) were obtained from testcrossed translocation trisomic parents. XXY males produced four types of gametes XY, X, Y and XX in equal numbers. XXX females only gave XX and X gametes in an equal number. Succesfull attempts to obtain homozygotes for this X-linked translocation are reported. The theoretical background of genetic insect control is discussed.

    Studies on dispersal of Adoxophyes orana F.v.R. in relation to the population sterilization technique
    Barel, C.J.A. - \ 1973
    Landbouwhogeschool Wageningen. Promotor(en): J. de Wilde. - Wageningen : Veenman - 107
    insecten - plantenplagen - vruchtbomen - boomgaarden - tortricidae - sterilisatie - steriele insecten techniek - genetische gewasbescherming - dieren - territorium - habitats - milieu - plantenziekten - epidemiologie - distributie - adoxophyes orana - microlepidoptera - insects - plant pests - fruit trees - orchards - tortricidae - sterilization - sterile insect release - genetic control - animals - territory - habitats - environment - plant diseases - epidemiology - distribution - adoxophyes orana - microlepidoptera

    In apple growing areas, in the Netherlands, Panonychus ulmi Koch and Adoxophyes orana FvR. are the most important pests. Preliminary experiments had shown that P. ulmi could be controlled by predators, but these predators are killed by insecticide sprayings against other pests.

    As A. orana had already been cultured on an artificial medium, the application of the population sterilization technique was within the realms of possibility. However, the high population density of the species was a disadventage. But if this was counterbalanced by a poor capacity for dispersal, the application of this technique, on a small scale, would be possible. In a simulation model, it was demonstrated that only 100 immigrants per generation per ha, was already too high to make the technique feasible.

    Three modes of dispersal could be expected: flight, aerial transport of larvae with wind and transport of larvae together with plants and packaging materials. The last possibility was not investigated, it was assumed that it could be reduced to a great extent by appropriate measures.

    Dispersal by flight was studied in release-recapture experiments. The released moths were cultured on a meridic diet. For marking, either 32P or the dye Calco oil red D was added to the medium. In order to recapture the moths, light traps and sex traps were used. The experiments were carried out in several places, an open field, orchards, and hedgerows. The results obtained indicated that the movement from the release points was small. The greatest distance at which a male was recaptured in these experiments was 250 m. Only in the experiment in which 75500 males were released homogeneously throughout an orchard of 1.5 ha, were the distances, at which males were still recovered, somewhat greater, but the maximum distance was 435 m. For this particular experiment it was calculated that about 1 to 2 percent of the males had left the orchard. It is assumed that, for this kind of biotope, this a representative percentage.

    In light traps, in general, the numbers of males captured were higher than the numbers of females captured. This could be the result of, either a lower flight activity of the females compared to the males, or an eventual difference in the diurnal flight period of males an females. In laboratory experiments, evidence was obtained for both possibilities.

    Concerning larval dispersal by means of wind, only qualitative data were obtained. It was demonstrated that it occurs frequently and that characteristic behaviour elements are involved. The larva spins a silken thread when it drops off from a leaf. This thread can be broken by the force of the wind, exerted on the thread. It subsequently breaks near the end at which it is attached to the leaf. The buoyancy of the larva in the air depends on the length of the thread. It was demonstrated that wind velocities of more than 3 m per sec have an immobilizing effect on the locomotion of the newly-hatched larvae. It is likely that this behaviour protects the larva against being blown away from the leaf, with a very short thread. Experiments on phototaxis and geotaxis yielded indications that the behaviour of young larvae makes them vulnerable to this mode of dispersal. The behaviour of older larvae was different in this respect. It is likely that, because of the greater weight, the chances for aerial transport of older larvae are reduced. In a qualitative way, aerial transport can be important. At 20 °C and 70 % R.H. the newly-hatched larva can survive 6 hours starvation. With an air speed of 2 m per sec, transport over more than 40 km is possible. The quantative aspects will be determined by the vegetation that surrounds the egg-mass. In an orchard, a large proportion of the larvae will be sieved out from the air, by the surrounding trees.

    A. orana has a wide hostplant range. In preliminary experiments the possibility of a variability of response towards different hostplants was investigated. No indications were found.

    When there is no variability in response towards apple, between A. orana in orchards and A. orana in other biotopes, it is important to know the population densities in these biotopes, especially in the fruit growing areas, where eventually the population sterilization technique is going to be applied. The population densities were estimated in 23 different places, hedgerows, lanes and woods, by releasing a known number of marked moths into the wild population. From the numbers recaptured by means of sex traps, the number of moths of the wild population was calculated, with the aid of the Lincoln-index. The density in woods was found to be lower than the average population density in orchards, but for some hedgerows higher densities were calculated. The calculated densities were highest when a great part of the hedgerows consisted of alder ( Alnus spp.) or hawthorn ( Crateagus sp.).

    The conclusion of these studies was, that with regard to the dispersal of the adults, there is a fair chance that the population sterilization technique can be applied on a small scale, when the area is surrounded by a zone of 500 m, free from other populations of A.orana. If this is not possible, sterilized adults also have to be released in these populations. In this conclusion, the dispersal of larvae by wind, is not taken into consideration. It is assumed that quantitatively, this mode of dispersal is of minor importance.

    Summer fruit tortrix moth, adoxophyes orana F.R. studies on biology, behaviour and population dynamics in relation to the application of the sterility principle
    Jong, D.J. de; Ankersmit, G.W. ; Barel, C.J.A. - \ 1971
    Wageningen : [s.n.] (Mededeling / Instituut voor plantenziektenkundig inderzoek no. 595) - 13
    insecten - plantenplagen - appels - peren - sterilisatie - steriele insecten techniek - genetische gewasbescherming - gewasbescherming - biologische bestrijding - straling - tortricidae - microlepidoptera - insects - plant pests - apples - pears - sterilization - sterile insect release - genetic control - plant protection - biological control - radiation - tortricidae - microlepidoptera
    Irradiation, competitiveness and the use of radioisotopes in sterile - male studies with the onion fly, Hylemya antiqua (Meigen); Radiation pathology in Hylemya antiqua (Meigen) : outlines of research; Rearing of the onion fly, Hylemya antiqua (Meigen), with a view to release of sterilized insects
    Noordink, J.P.W. ; Theunissen, J. ; Ticheler, J. - \ 1971
    Wageningen : [s.n.] (Mededeling / Instituut voor plantenkundig onderzoek no. 555, 556, 557) - 22
    sterilisatie - insecten - steriele insecten techniek - genetische gewasbescherming - biologische bestrijding - natuurlijke vijanden - massakweek - anthomyiidae - muscidae - drosophilidae - calliphoridae - agromyzidae - tachinidae - glossinidae - tephritidae - radioactiviteit - effecten - biologie - voortplanting - schizophora - sterilization - insects - sterile insect release - genetic control - biological control - natural enemies - mass rearing - anthomyiidae - muscidae - drosophilidae - calliphoridae - agromyzidae - tachinidae - glossinidae - tephritidae - radioactivity - effects - biology - reproduction - schizophora
    New elements for integration in pest control schedules
    Wilde, J. de - \ 1968
    Wageningen : [s.n.] (Mededeling / Laboratorium voor entomologie no. 163) - 8
    gewasbescherming - biologische bestrijding - sterilisatie - insecten - steriele insecten techniek - genetische gewasbescherming - insecticiden - acariciden - mollusciciden - plant protection - biological control - sterilization - insects - sterile insect release - genetic control - insecticides - acaricides - molluscicides
    Application of the sterile - male technique on the onion-fly, Hylemya antiqua(Meig.), in the Netherlands. Progress report
    Ticheler, J. ; Noordink, J.P.W. - \ 1968
    Wageningen : [s.n.] (Mededeling / Instituut voor plantenziektenkundig onderzoek no. 489) - 5
    schimmel - wortelrot - voetrot - smeul - sterilisatie - insecten - steriele insecten techniek - genetische gewasbescherming - plantenplagen - Anthomyiidae - Muscidae - Drosophilidae - Calliphoridae - Agromyzidae - Tachinidae - Glossinidae - Tephritidae - Nederland - schizophora - moulds - root rots - foot rot - damping off - sterilization - insects - sterile insect release - genetic control - plant pests - Anthomyiidae - Muscidae - Drosophilidae - Calliphoridae - Agromyzidae - Tachinidae - Glossinidae - Tephritidae - Netherlands - schizophora
    Bibliographies on non - chemical insect control
    Anonymous, - \ 1968
    Wageningen : [s.n.] (Literatuurlijst / Centrum voor landbouwpublikaties en landbouwdocumentatie no. 3001)
    bibliografieën - biologische bestrijding - bestrijdingsmethoden - genetische gewasbescherming - insecten - geïntegreerde bestrijding - geïntegreerde plagenbestrijding - plantenziekten - plantenplagen - gewasbescherming - steriele insecten techniek - sterilisatie - bibliographies - biological control - control methods - genetic control - insects - integrated control - integrated pest management - plant diseases - plant pests - plant protection - sterile insect release - sterilization
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