Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Mobile and immobile adhesion of staphylococcal strains to hydrophilic and hydrophobic surfaces
    Boks, N.P. ; Kaper, H.J. ; Norde, W. ; Mei, H.C. van der; Busscher, H.J. - \ 2009
    Journal of Colloid and Interface Science 331 (2009)1. - ISSN 0021-9797 - p. 60 - 64.
    bacterial adhesion - biofilm reactors - infections - attachment - deposition - reversibility - substrata - energies - system - flow
    Staphylococcus epidermidis adheres to hydrophilic glass and hydrophobic dimethyldichlorosilane (DDS)-coated glass in similar numbers, but in different modes. Real-time observation of staphylococcal adhesion under a shear rate of 15 s(-1) revealed different adhesion dynamics on both substrata. The number of adsorption and desorption events to achieve a similar number of adhering bacteria was twofold higher on hydrophilic than on hydrophobic DDS-coated glass. Moreover. 22% of all staphylococci on glass slid over the surface prior to adhering on a fixed site ("mobile adhesion mode"), but mobile adhesion was virtually absent (1%) on DDS-coated glass. Sliding preceded desorption on hydrophilic glass in about 20% of all desorption events, while on hydrophobic DDS-coated glass 2% of all staphylococci desorbed straight from their adhesion site. Since acid-base interactions between the staphylococci and a hydrophobic DDS-coating are attractive, it is suggested that these interactions facilitate a closer approach of the bacteria and therewith enhance immobile adhesion at local, high affinity sites. Alternatively, if the local site is low affinity, this may lead to desorption. In the absence of attractive acid-base interactions, as on hydrophilic glass, bacteria can be captured in the minimum of the DLVO-interaction energy curve, but this does not prevent them from sliding under flow at a fixed distance from a substratum surface until immobilization or desorption at or from a local high or low affinity site, respectively.
    Acoustic habitat and shellfish mapping and monitoring in shallow coastal water - Sidescan sonar experiences in The Netherlands
    Overmeeren, R. van; Craeymeersch, J.A.M. ; Dalfsen, J. van; Fey-Hofstede, F.E. ; Heteren, S. ; Meesters, E. - \ 2009
    Estuarine Coastal and Shelf Science 85 (2009)3. - ISSN 0272-7714 - p. 437 - 448.
    english-channel - benthic habitat - classification - substrata - regions
    Sidescan sonar has been applied in a number of shallow water environments along the Dutch coast to map and monitor shellfish and seabed habitats. The littoral setting of these surveys may hamper data acquisition flying the towfish in zones of turbulence and waves, but also offers valuable opportunities for understanding, interpreting and validating sidescan sonar images because of the ability to ground-truth during low water periods, enabling easy identification and validation. Acoustical images of some of the mussel banks on the tidal flats of the Wadden Sea, recorded at high tide, show a marked resemblance with optical Google Earth images of the same banks. These sonar images may thus serve as 'acoustic type signatures' for the interpretation of sonar patterns recorded in deeper water where ground-truthing is more difficult and more expensive. Similarly, acoustic type signatures of (Japanese) oyster banks were obtained in the estuaries in the southwest of the Netherlands. Automated acoustic pattern recognition of different habitats and acoustical estimation of faunal cover and density are possible applications of sidescan sonar. Both require that the backscattering observed on the sidescan sonar images is directly caused by the biological component of the seafloor. Filtering offers a simple and effective pre-processing technique to separate the faunal signals from linear trends such as emanating from wave ripples or the central tracks of the towfish. Acoustically estimating the faunal density is approached by in-situ counting peaks in backscattering in unit squares. These counts must be calibrated by ground-truthing. Ground-truthing on littoral mussel banks in the Wadden Sea has been carried out by measuring their cover along lines during low tide. Due to its capacity of yielding full-cover, high resolution images of large surfaces, sidescan sonar proves to be an excellent, cost-effective tool for quantitative time-lapse monitoring of habitats.
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