Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Cyclosporine A treated in vitro models induce cholestasis response through comparison of phenotype-directed gene expression analysis of in vivo Cyclosporine A-induced cholestasis
    Kienhuis, A.S. ; Vitins, A.P. ; Pennings, J.L.A. ; Pronk, T.E. ; Speksnijder, E.N. ; Roodbergen, M. ; Delft, J.H.M. van; Luijten, M. ; Ven, L.T.M. van der - \ 2013
    Toxicology Letters 221 (2013)3. - ISSN 0378-4274 - p. 225 - 236.
    proliferator-activated receptor - phospholipid transfer protein - nuclear receptors - bile-acids - liver - hepatotoxicity - transporters - mechanisms - mice - drugs
    In vitro models for hepatotoxicity testing are a necessity for advancement of toxicological research. Assessing the in vitro response requires in vivo validated gene sets reflective of the hepatotoxic phenotype. Cholestasis, the impairment of bile flow, is induced in C57BL/6J mice treated with cyclosporine A (CsA) to identify phenotype reflective gene sets. CsA treatment through oral gavage for 25 days induced cholestasis, as confirmed by histopathology and serum chemistry. Over 1, 4, and 11 days of CsA exposure gradual increases in serum markers were correlated to gene expression. This phenotype-directed analysis identified gene sets specific to the onset and progression of cholestasis, such as PPAR related processes and drug metabolism, by circumventing other effects of CsA, such as immunosuppression, found in dose*time group analysis. In vivo gene sets are enriched in publicly available data sets of CsA-treated HepaRG and primary mouse hepatocytes. However, genes identified within these gene sets did not overlap between in vivo and in vitro. In vitro regulated genes represent the initial response to cholestasis, whereas in vivo genes represent the later adaptive response. We conclude that the applicability of in vitro models for hepatotoxicity testing fully depends on a solid in vivo phenotype anchored analysis. (c) 2013 Elsevier Ireland Ltd. All rights reserved.
    Interaction of hesperetin glucuronide conjugates with human BCRP, MRP2 and MRP3 as detected in membrane vesicles of overexpressing baculovirus-infected Sf9 cells
    Brand, W. ; Oosterhuis, B. ; Krajcsi, P. ; Barron, D. ; Dionisi, F. ; Bladeren, P.J. van; Rietjens, I. ; Williamson, G. - \ 2011
    Biopharmaceutics and Drug Disposition 32 (2011)9. - ISSN 0142-2782 - p. 530 - 535.
    phase-ii metabolism - disposition - monolayers - hesperidin - bioavailability - transporters - absorption - flavanones - flavonoids - fruit
    The citrus flavonoid hesperetin (4'-methoxy-3',5,7-trihydroxyflavanone) is the aglycone of hesperidin, the major flavonoid present in sweet oranges. Hesperetin 7-O-glucuronide (H7G) and hesperetin 3'-O-glucuronide (H3'G) are the two most abundant metabolites of hesperetin in vivo. In this study, their interaction with specific ABC transporters, believed to play a role in the disposition and bioavailability of hesperetin, was studied using Sf9 membranes from cells overexpressing human BCRP (ABCG2), MRP2 (ABCC2) and MRP3 (ABCC3). Both H7G and H3'G were tested for their potential to activate and inhibit ATPase activity, and to inhibit vesicular transport by these transporters. Both H7G and H3'G demonstrated interaction with all tested ABC transporters, especially with BCRP and MRP3. An interesting difference between H7G and H3'G was seen with respect to the interaction with BCRP: H7G stimulated the ATPase activity of BCRP up to 76% of the maximal effect generated by the reference activator sulfasalazine, with an EC(50) of 0.45¿µ m, suggesting that H7G is a high affinity substrate of BCRP, whereas H3'G did not stimulate BCRP ATPase activity. Only moderate inhibition of BCRP ATPase activity at high H3'G concentrations was observed. This study provides information on the potential of hesperetin glucuronide conjugates to act as specific ABC transporter substrates or inhibitors and indicates that regio-specific glucuronidation could affect the disposition of hesperetin.
    Air tightness and heat leakage in insulated bodies
    Lukasse, L.J.S. ; Voort, A.J. van der; Ploegaert, J.P.M. - \ 2006
    Wageningen : Agrotechnology & Food Innovations (Report / Wageningen UR, Agrotechnology & Food Innovations 586) - ISBN 9789067549912 - 10
    ventilatie - luchtdichte opslag - lekkage - warmteoverdracht - isolatie (insulation) - koeltransport - containers - transportvoertuigen - ventilation - airtight storage - leakage - heat transfer - insulation - refrigerated transport - containers - transporters
    Associative Nitrogen Fixation and Root Exudation - What is Theoretically Possible in the Rhizosphere?
    Jones, D.L. ; Farrar, J. ; Giller, K.E. - \ 2003
    Symbiosis 35 (2003)1-3. - ISSN 0334-5114 - p. 19 - 38.
    zea-mays-l - organic-compounds - re-sorption - carbon-flow - amino-acids - wheat roots - soil - transporters - malate - plants
    Root exudation is a key driver of many rhizosphere processes including nitrogen fixation by diazotrophic bacteria residing in the soil. We critically review our knowledge of rhizosphere carbon flow and determine the extent to which rhizodeposition could fuel associative N2 fixation by soil microorganisms. We conclude that most estimates of rhizosphere C flow are fundamentally flawed due to the use of inappropriate methodology combined with a poor mechanistic understanding of root C flow. Using a mathematical model, we predicted that rhizodeposition could under optimal conditions support the fixation of between 0.2 to 4 kg N ha-1 year-1 which is in good agreement with experimentally derived values for natural ecosystems (0.05 to 5 kg N ha-1 y-1). Our model indicated that fixation was highly dependent upon the number of potential N2 fixers in the rhizosphere relative to the total microbial population. If N2 fixer populations could be enhanced, we predict that fixation rates may reach up to 20 kg N ha-1 y-1 given highly optimal conditions which again agree with experimentally derived results. We conclude that whilst the potential for rhizodeposition-driven N2 fixation in the soil is small in comparison to inorganic and symbiotic-N2 fixation inputs, it may be of importance in N-limited ecosystems.
    ABC transporters of the wheat pathogen Mycosphaerella graminicola
    Zwiers, L.H. - \ 2002
    Wageningen University. Promotor(en): P.J.G.M. de Wit; M.A. de Waard. - S.l. : S.n. - ISBN 9789058086136 - 127
    mycosphaerella graminicola - tarwe - plantenziekteverwekkende schimmels - bindende eiwitten - atp - meervoudige resistentie tegen geneesmiddelen - transportvoertuigen - genexpressie - triticum aestivum - transformatie - wheat - triticum aestivum - mycosphaerella graminicola - plant pathogenic fungi - binding proteins - transporters - atp - multiple drug resistance - gene expression - transformation

    A TP- b inding c assette (ABC) transporters belong to one of the largest protein families known. They play a role in numerous vital processes in the cell and are characterised by their capacity to transport a broad variety of substrates, ranging from simple ions to complex polypeptides. Many human diseases are correlated to malfunctioning of ABC transporters. Historically, ABC transporters became known because of their role in the development of multidrug resistance (MDR) during cancer therapy. MDR is the simultaneous development of resistance against chemically unrelated compounds and can be the consequence of overproduction of ABC transporters. MDR is not limited to human tumour cells but also occurs during chemical treatment of parasites, bacteria, and fungi. In plant pathogenic fungi ABC transporters can play a role in resistance against azole fungicides. Chapter 1 gives an overview of common functions and characteristics of ABC transporters.

    This thesis describes the functional analysis of ABC transporters in Mycosphaerella graminicola, one of the most important pathogens on wheat. This fungus is the causal agent of septoria tritici leaf blotch. When not properly controlled, yield losses caused by this disease can be as high as 50%. Despite its importance, not much is known about the molecular biology of this fungus. Chapter 2 describes the cloning of the first two ABC transporter genes of this fungus, MgAtr1 and MgAtr2 . Both genes were isolated by means of heterologous hybridisation using a probe derived from the Saccharomyces cerevisiae ABC transporter PDR5 . Exposure of M. graminicola to a broad variety of chemicals showed that several compounds enhanced expression of one or both genes. Compounds capable of increasing the expression include antibiotics, plant secondary metabolites, and fungicides used to control M. graminicola , suggesting a role for MgAtr1 and MgAtr2 in protection against these compounds. Another striking result is the differential expression of the two genes in yeast-like cells and mycelium, suggesting a morphology dependent regulation of expression.

    Chapter 3 describes the development of an Agrobacterium tumefaciens -mediated transformation protocol. In contrast to several other methods, A. tumefaciens -mediated transformation is highly effective and results in the efficient generation of disruption mutants. The establishment of this transformation protocol enabled the functional analysis of ABC transporters of M. graminicola by gene disruption.

    Independent knockout mutants were generated for five ABC transporter genes from M. graminicola and the virulence of these mutants was tested on wheat. As described in Chapter 4,DMgAtr4 mutants were less virulent, while all the other transformants showed unaltered virulence. However, it is still unclear how MgAtr4 exerts its action. MgAtr4 is the first described virulence factor of M. graminicola and the fourth ABC transporter described as a virulence factor in plant pathogenic fungi.

    The substrate specificity of the ABC transporters was studied by complementation of S. cerevisiae mutants with the M. graminicola ABC transporter genes and by analysis of the M . graminicola knockout mutants for sensitivity to a broad variety of compounds. The results of the yeast complementation assay presented in Chapter 5 clearly show that the ABC transporters tested function as multidrug transporters with overlap in substrate specificity. The substrate range in yeast includes antibiotics, fungicides, plant secondary metabolites, and a mycotoxin. The M. graminicola knockout mutants did not show altered sensitivity to any of these potential substrates. This can be explained by the fact that multiple ABC transporters exist with overlap in substrate specificity, which can take over the function of the distorted transporter. MgAtr5 deletion mutants showed a small increase in sensitivity to the putative wheat defence compound resorcinol, suggesting a role for MgAtr5 in the M. graminicola -wheat pathosystem. Bioassays to test the effect of antagonistic bacteria on the growth of the ABC transporter knockout mutants, indicate that MgAtr2 can provide protection against antibiotics produced by these bacteria. Thus, the data presented in Chapter 5 indicate that ABC transporters from M. graminicola play a role in the protection against toxic compounds.

    Both the expression analysis (Chapter 2) and the yeast complementation data (Chapter 5) suggest that azole fungicides can act as substrates for ABC transporters from M . graminicola . In Chapter 6 the potential role of ABC transporters in fungicide sensitivity is studied in more detail. M. graminicola mutants with decreased sensitivity to the azole fungicide cyproconazole were generated and shown to exhibit a MDR phenotype. Decreased azole sensitivity correlated with altered accumulation of cyproconazole, indicative for the involvement of ABC transporters. Expression of one or more of the ABC transporter genes studied was altered in all mutants. However, changes in fungicide sensitivity did not correlate

    with alterations in expression of a specific ABC transporter gene. Disruption of MgAtr1 in two mutants showing constitutive MgAtr1 overexpression restored cyproconazole sensitivity to wild-type levels in only one of these mutants. These results show that overexpression of ABC transporters is one of the mechanisms leading to azole resistance in M. graminicola .

    In conclusion, the data presented in this thesis show that M. graminicola possesses ABC transporters with overlapping substrate specificity. Substrates include xenobiotics and natural toxicants. This is confirmed by the findings that ABC transporters from M . graminicola act as virulence factor and can provide protection against mycotoxins, bacterial antibiotics, and azole fungicides. Therefore, ABC transporters of M . graminicola contribute to the success of this fungus as a pathogen.

    Specifiek brandstofverbruik en vermogensbehoefte van trekkers = Specific fuel consumption and required power of tractors
    Straelen, B.C.P.M. van; Maeyer, E.E.A. de - \ 1997
    Wageningen : IMAG-DLO (Rapport / Dienst Landbouwkundig Onderzoek, Instituut voor Milieu- en Agritechniek 97-10) - ISBN 9789054061588 - 33
    trucks - trekkers - transportvoertuigen - kracht - trekkrachtefficiëncy - prestatieniveau - motoren - technologie - brandstoffen - vloeistoffen (liquids) - mineraaloliën - verbrandingsmotoren - dieselmotoren - aardolie - dieselolie - stookoliën - energie - efficiëntie - energiegebruik - brandstofverbruik - tractors - transporters - power - tractive efficiency - performance - engines - technology - fuels - liquids - mineral oils - internal combustion engines - diesel engines - petroleum - diesel oil - fuel oils - energy - efficiency - energy consumption - fuel consumption
    Bemonstering van drijfmest in transportwagens
    Hoeksma, P. ; Ogink, N.W.M. ; Derikx, P.J.L. ; Willems, G.W.M. - \ 1995
    Wageningen : IMAG-DLO - ISBN 9789054061137 - 38
    dierlijke meststoffen - basisproducten - boerderij uitrusting - landbouwwerktuigen - stalmest - mest - marketing - monstertrekkers - drijfmest - gereedschappen - transport - transportvoertuigen - trucks - animal manures - commodities - farm equipment - farm machinery - farmyard manure - manures - samplers - slurries - tools - transporters
    Containersystemen ook bij hoog oogsten
    Hendrix, A.T.M. - \ 1995
    Groenten en Fruit. Vakdeel glasgroenten (1995)22. - ISSN 1380-3573 - p. 12 - 13.
    capsicum annuum - containers - harvesting - transport - transporters - sweet peppers - machines - oogsten - transportvoertuigen - paprika
    Ter verbetering van de arbeidsomstandigheden gebruikt een behoorlijk aantal telers containers bij de oogst van paprika. Dit levert een arbeidsbesparing op die groter is dan de jaarkosten van de noodzakelijke investeringen. Een nadeel van sommige containersystemen is echter het grote aantal vruchtbeschadigingen
    Ergonomische beoordeling exportkontainer 555
    Hendrix, A.T.M. - \ 1991
    Wageningen : DLO-Instituut voor Mechanisatie, Arbeid en Gebouwen (Nota / DLO Instituut voor Mechanisatie, Arbeid en Gebouwen 91-47) - 12
    bedrijven - containers - distributie - ondernemingen - ergonomie - bloemen - bewerking - apparatuur voor laden en lossen - pallets - bederfelijke producten - beplanten - struiken - transport - transportvoertuigen - bomen - logistiek - businesses - distribution - enterprises - ergonomics - flowers - handling - handling machinery - perishable products - planting - shrubs - transporters - trees - logistics
    Naar een optimaal vervoer en opslag van enkele groenten- en fruitsoorten (een literatuuronderzoek)
    Gerritsen, A. ; Nieuwenhuizen, G.H. van - \ 1988
    Wageningen : Sprenger Instituut (Rapport / Sprenger Instituut no. 569) - 178
    fruitteelt - tuinieren - sierplanten - aardappelen - overzichten - solanum tuberosum - opslag - transport - transportvoertuigen - groenteteelt - fruit growing - gardening - ornamental plants - potatoes - reviews - storage - transporters - vegetable growing
    Temperatuurmetingen aan een separatiezeil in een AH voertuig : losprocedures
    Laar, H.J. van; Nieuwenhuizen, G.H. van - \ 1985
    Wageningen : Sprenger Instituut (Rapport / Sprenger Instituut no. 2292) - 15
    voedsel - voedselbewaring - voedingsmiddelen - koeltransport - transportvoertuigen - food - food preservation - foods - refrigerated transport - transporters
    Onderzoek aan dunwandige koelvoertuigen in een teststation en tijdens vervoer
    Nieuwenhuizen, G.H. van; Meffert, H.F.T. - \ 1985
    Wageningen : Sprenger Instituut (Rapport / Sprenger Instituut no. 2294)
    koeltransport - transportvoertuigen - refrigerated transport - transporters
    Test van een transportauto voor het gekoeld vervoer van champignons
    Verbeek, W. ; Bons, R.G. - \ 1985
    Wageningen : Sprenger Instituut (Rapport / Sprenger Instituut no. 2309) - 8
    transport - agaricus - transportvoertuigen - koeltransport - transporters - refrigerated transport
    Afkoelproef met snijbloemen met het Veko voorkoelsysteem in een lijnrijauto
    Boerrigter, H.A.M. ; Damen, P.M.M. - \ 1980
    Wageningen : Sprenger Instituut (Rapport / Sprenger Instituut no. 2103) - 12
    koelcellen - koelen - warmtewisselaars - sierplanten - koeltransport - koudeopwekking - transportvoertuigen - cold stores - cooling - heat exchangers - ornamental plants - refrigerated transport - refrigeration - transporters
    Pneumatic conveying of forage when loading tower silo's
    Bosma, A.H. - \ 1979
    Wageningen : I.M.A.G. (Research report / Institute of Agricultural Engineering. I.M.A.G. [no.] 79-2) - 20
    transportbanden - transporteerapparatuur - voer - voedergewassen - laders - pneumatische transporteurs - silo's - opslagloodsen - transportvoertuigen - loswerktuigen - belt conveyors - conveyors - feeds - fodder crops - loaders - pneumatic conveyors - silos - stores - transporters - unloaders
    Onderzoek naar de blaaswerking en naar mogelijke verbeteringen bij het vullen van torensilo's
    Aspecten van het buisrailsysteem
    Ammerlaan, J.C.J. - \ 1978
    Naaldwijk : Proefstation voor de Groenten- en Fruitteelt onder Glas (Informatiereeks / Proefstation voor de groenten- en fruitteelt onder glas en consulentschap voor de tuinbouw no. 52) - 25
    teelt onder bescherming - tuinbouw - kassen - forceren van planten - transport - transportvoertuigen - landbouwtechniek - boerderij uitrusting - landbouwwerktuigen - glastuinbouw - protected cultivation - horticulture - greenhouses - forcing - transport - transporters - agricultural engineering - farm equipment - farm machinery - greenhouse horticulture
    Een isolatiewaardemeting in duplo aan een Heiwo koelcarrosserie in samenwerking met de Rijksdienst voor het wegverkeer
    Nieuwenhuizen, G.H. ; Laar, H.J. van - \ 1978
    Wageningen : Sprenger Instituut (Rapport / Sprenger instituut no. 2018) - 15
    koeltransport - transportvoertuigen - refrigerated transport - transporters
    Pneumatisch transport van ruwvoer bij het vullen van torensilo's
    Bosma, A.H. - \ 1978
    Wageningen : IMAG (Publikatie / Instituut voor mechanisatie, arbeid en gebouwen no. 94) - 56
    transportbanden - transporteerapparatuur - voer - voedergewassen - laders - kuilsilo's - pneumatische transporteurs - silo's - opslagloodsen - torensilo's - transportvoertuigen - loswerktuigen - belt conveyors - conveyors - feeds - fodder crops - loaders - pit silos - pneumatic conveyors - silos - stores - tower silos - transporters - unloaders
    Kurzpruefmethode fuer Kuehlercontainer und Kuehlfahrzeuge : Erstellung der Pruefvorschrift
    Baumgartner, G. - \ 1977
    Wageningen : Sprenger Instituut (Rapport / Sprenger instituut no. 1983) - 39
    koeltransport - transportvoertuigen - refrigerated transport - transporters
    Vervoer van aardappelen in koelwagens met residu - ijs
    Meijers, C.P. ; Rastovski, A. ; Schooneveld, B. - \ 1977
    Wageningen : I.B.V.L. (Publikatie / Inst. voor bewaring en verwerking van landbouwprodukten) no. 309) - 4
    plantaardige producten - aardappelen - railtransport - koeltransport - solanum tuberosum - transport - transportvoertuigen - behandeling - voertuigen - plant products - potatoes - rail transport - refrigerated transport - transporters - treatment - vehicles
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