Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    GpaXIltar originating from Solanum tarijense is a major resistance locus to Globodera pallida and is localised on chromosome 11 of potato
    Tan, M.Y.A. ; Park, T.H. ; Alles, R. ; Hutten, R.C.B. ; Visser, R.G.F. ; Eck, H.J. van - \ 2009
    Theoretical and Applied Genetics 119 (2009)8. - ISSN 0040-5752 - p. 1477 - 1487.
    broad-spectrum resistance - comigrating aflp markers - cyst-nematode - confers resistance - gene-cluster - tuberosum - linkage - tomato - virus - dna
    Resistance to Globodera pallida Rookmaker (Pa3), originating from wild species Solanum tarijense was identified by QTL analysis and can be largely ascribed to one major QTL. GpaXI tar l explained 81.3% of the phenotypic variance in the disease test. GpaXI tar l is mapped to the long arm of chromosome 11. Another minor QTL explained 5.3% of the phenotypic variance and mapped to the long arm of chromosome 9. Clones containing both QTL showed no lower cyst counts than clones with only GpaXI tar l . After Mendelising the phenotypic data, GpaXI tar l could be more precisely mapped near markers GP163 and FEN427, thus anchoring GpaXI tar l to a region with a known R-gene cluster containing virus and nematode resistance genes
    High-resolution mapping and analysis of the resistance locus Rpi-abpt against Phytophthora infestans in potato
    Park, T.H. ; Vleeshouwers, V.G.A.A. ; Hutten, R.C.B. ; Eck, H.J. van; Vossen, E.A.G. van der; Jacobsen, E. ; Visser, R.G.F. - \ 2005
    Molecular Breeding 16 (2005)1. - ISSN 1380-3743 - p. 33 - 43.
    broad-spectrum resistance - comigrating aflp markers - solanum-bulbocastanum - late-blight - cultivated potato - tomato genome - linkage maps - r-genes - tuberosum - arabidopsis
    Introduction of more durable resistance against Phytophthora infestans causing late blight into the cultivated potato is of importance for sustainable agriculture. We identified a new monogenically inherited resistance locus that is localized on chromosome 4. The resistance is derived from an ABPT clone, which is originally a complex quadruple hybrid in which Solanum acaule, S. bulbocastanum, S. phureja and S. tuberosum were involved. Resistance data of the original resistant accessions of the wild species and analysis of mobility of AFLP markers linked to the resistance locus suggest that the resistance locus is originating from S. bulbocastanum. A population of 1383 genotypes was screened with two AFLP markers flanking the Rpi-abpt locus and 98 recombinants were identified. An accurate high-resolution map was constructed and the Rpi-abpt locus was localized in a 0.5 cM interval. One AFLP marker was found to co-segregate with the Rpi-abpt locus. Its DNA sequence was highly similar with sequences found on a tomato BAC containing several resistance gene analogues on chromosome 4 and its translated protein sequence appeared to be homologous to several disease resistance related proteins. The results indicated that the Rpi-abpt gene is a member of an R gene cluster
    Epidemiological importance of Solanum sisymbriifolium, S. nigrum and S. dulcamara as alternative hosts for Phytophthora infestans
    Flier, W.G. ; Bosch, G.B.M. van den; Turkensteen, L.J. - \ 2003
    Plant Pathology 52 (2003)5. - ISSN 0032-0862 - p. 595 - 603.
    potato cyst nematodes - hypersensitive response - population-structure - toluca valley - resistance - netherlands - tuberosum - migration - demissum - hybrids
    Lesions of Phytophthora infestans were found on woody nightshade (Solanum dulcamara), black nightshade (S. nigrum) and S. sisymbriifolium during a nationwide late blight survey in the Netherlands in 1999 and 2000. Pathogenicity and spore production of P. infestans isolates collected from potato (S. tuberosum), S. nigrum, S. dulcamara and S. sisymbriifolium were determined on several host plant species, and oospore formation in naturally infected and inoculated foliage of hosts was quantified. The present population of P. infestans in the Netherlands is pathogenic on S. nigrum, S. dulcamara and S. sisymbriifolium. Oospores were produced in leaves of S. nigrum, S. dulcamara and S. sisymbriifolium following infection with A1 and A2 isolates. Therefore these plant species should be regarded as alternative hosts for the late blight pathogen. In the case of S. nigrum and S. dulcamara infection was a relatively rare event, suggesting that diseased plants do not significantly contribute to the overall late blight disease pressure present in potato-production areas. Oospore production in ageing S. nigrum and S. dulcamara plants in autumn, however, may generate a considerable source of (auto) infections in following years. Considerable numbers of sporangia and oospores were produced on S. sisymbriifolium following infection with P. infestans. Additional field infection data are needed to evaluate the epidemiological consequences of a commercial introduction of S. sisymbriifolium as a potato cyst nematode trap crop
    Alteration of the genomic composition of Solanum nigrum (+) potato backcross derivatives by somatic hybridisation: selection of fusion hybrids by DNA measurements and GISH
    Horsman, K. ; Gavrilenko, T. ; Bergervoet, M. ; Huigen, D.J. ; Tjin Wong Joe, A. ; Jacobsen, E. - \ 2001
    Plant Breeding 120 (2001)3. - ISSN 0179-9541 - p. 201 - 207.
    infestans mont debary - lycopersicon-esculentum - chromosome-number - alien chromosomes - rflp analysis - tuberosum - brevidens - tomato - identification - resistance
    Fusion experiments were performed with a first (BC1-6738) and a second (BC2-9017) generation backcross hybrid of 6x Solanum nigrum (+) 2x potato somatic hybrids with potato cultivars. Because no progeny was obtained from the BC2 genotypes, alternative approaches were sought to overcome the sexual crossing barrier. Five potato genotypes, one of which contains the hygromycin resistance gene, were used in the fusion experiments. All vigorous regenerants were used for the estimation of nuclear DNA content using flow cytometry. Plants with a DNA content higher than that of the BC1-6738 or BC2 genotypes were considered potential somatic hybrids. Forty-nine potential somatic hybrids resulted from fusion experiments with BC1-6738, from which 20 grew vigorously in the greenhouse and flowered. After pollination with several 4x potato cultivars. eight genotypes produced seeded berries and five genotypes gave seedless berries. In addition. Il of these 13 somatic hybrids were selected for genomic in situ hybridization (GISH) analysis to determine their genomic composition. Nine had exactly or approximately the expected number of 36 S. nigrum and 60 potato chromosomes. In one genotype. only 22 instead of 36 S. nigrum chromosomes were found acid one potato chromosome was possibly missing. Only five potential somatic hybrids were detected among the 79 regenerants from BC2-9017 (+) 2x potato Fusion experiments that were analysed by flow cytometry. Two of these hybrids were rather vigorous and did flower, but pollinations with potato have not yet yet any berries.
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