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Quantitative in vitro-to-in vivo extrapolation (QIVIVE) of estrogenic and anti-androgenic potencies of BPA and BADGE analogues
Punt, Ans ; Aartse, Aafke ; Bovee, Toine F.H. ; Gerssen, Arjen ; Leeuwen, Stefan P.J. van; Hoogenboom, Ron L.A.P. ; Peijnenburg, Ad A.C.M. - \ 2019
Archives of Toxicology 93 (2019)7. - ISSN 0340-5761 - p. 1941 - 1953.
Androgenic - Bisphenol A - BP analogues - Estrogenic - QIVIVE - Relative potencies
The goal of the present study was to obtain an in vivo relevant prioritization method for the endocrine potencies of different polycarbonate monomers, by combining in vitro bioassay data with physiologically based kinetic (PBK) modelling. PBK models were developed for a selection of monomers, including bisphenol A (BPA), two bisphenol F (BPF) isomers and four different bisphenol A diglycidyl ethers (BADGEs), using in vitro input data. With these models, the plasma concentrations of the compounds were simulated, providing means to estimate the dose levels at which the in vitro endocrine effect concentrations are reached. The results revealed that, whereas the in vitro relative potencies of different BADGEs (predominantly anti-androgenic effects) can be up to fourfold higher than BPA, the estimated in vivo potencies based on the oral equivalent doses are one to two orders of magnitude lower than BPA because of fast detoxification of the BADGEs. In contrast, the relative potencies of 2,2-BPF and 4,4-BPF increase when accounting for the in vivo availability. 4,4-BPF is estimated to be fivefold more potent than BPA in humans in vivo in inducing estrogenic effects and both 2,2-BPF and 4,4-BPF are estimated to be, respectively, 7 and 11-fold more potent in inducing anti-androgenic effects. These relative potencies were considered to be first-tier estimates, particularly given that the potential influence of intestinal metabolism on the in vivo availability was not accounted for. Overall, it can be concluded that both 2,2-BPF and 4,4-BPF are priority compounds.
De bepaling van diersoorten in vleesmengsels met DNA-gebaseerde qPCR-methoden
Aartse, A. ; Scholtens, I.M.J. ; Kok, E.J. - \ 2017
Wageningen : RIKILT Wageningen University & Research (RIKILT-rapport 2017.009) - 91
Evaluation of a loop-mediated isothermal amplification (LAMP) method for rapid on-site detection of horse meat
Aartse, Aafke ; Scholtens-Toma, Ingrid ; A, Hans J.G. van der; Boersma-Greve, Monique M. ; Prins, Theo W. ; Ginkel, Leen A. van; Kok, Esther J. ; Bovee, Toine F.H. - \ 2017
Food Control 81 (2017). - ISSN 0956-7135 - p. 9 - 15.
Horse meat - Loop-mediated isothermal amplification (LAMP) - On-site detection - qPCR - Rapid method
Detection of horse DNA by loop-mediated isothermal amplification (LAMP) seems one of the most promising methods to meet the criteria of fast, robust, cost efficient, specific, and sensitive on-site detection. In the present study an assessment of the specificity and sensitivity of the LAMP horse screening assay was made and outcomes were compared with the EURL-AP (European Union Reference laboratory for Animal Proteins in feeding stuffs) qPCR method. The specificity was tested with DNA samples from seven other species. The sensitivity of the LAMP assay was subsequently challenged with different percentages of horse DNA in cattle DNA and different percentages of horse meat in cattle meat. Both qPCR and LAMP were able to reliably detect horse DNA or meat below 0.1%, but LAMP was able to do so in less than 30 min. The DNA of other species did not result in a response in the LAMP horse assay. These features show that the LAMP method is fast, specific, and sensitive. Next, 69 processed meat samples were screened for the presence of horse DNA. The results showed that the LAMP horse assay, combined with a simple and fast on-site DNA extraction method, results in similar outcomes as the EURL-AP qPCR method and is thus a promising screening assay to be used outside the laboratory. Only samples that are screened on-site as suspect in the LAMP horse assay, need to be brought to the laboratory for confirmation with the more laborious EURL-AP qPCR reference method.
Widespread Genomic Incompatibilities in Caenorhabditis elegans
Snoek, L.B. ; Orbidans, H.E. ; Stastna, J. ; Aartse, A. ; Rodriguez Sanchez, M. ; Riksen, J.A.G. ; Kammenga, J.E. ; Harvey, S.C. - \ 2014
G3 : Genes Genomes Genetics 4 (2014)10. - ISSN 2160-1836 - p. 1813 - 1823.
cryptic genetic-variation - natural variation data - life-history traits - c. elegans - outbreeding depression - hybrid sterility - reproductive isolation - tribolium-castaneum - population-genetics - synthetic lethals
In the Bateson-Dobzhansky-Muller (BDM) model of speciation, incompatibilities emerge from the deleterious interactions between alleles that are neutral or advantageous in the original genetic backgrounds, i.e. negative epistatic effects. Within species such interactions are responsible for outbreeding depression and F2 (hybrid) breakdown. We sought to identify BDM incompatibilities in the nematode Caenorhabditis elegans by looking for genomic regions that disrupt egg laying; a complex, highly regulated and coordinated phenotype. Investigation of introgression lines and recombinant inbred lines derived from the isolates CB4856 and N2 uncovered multiple incompatibility quantitative trait loci (QTL). These QTL produce a synthetic egg-laying defective phenotype not seen in CB4856 and N2 nor in other wild isolates. For two of the QTL regions, results are inconsistent with a model of pairwise interaction between two loci, suggesting that the incompatibilities are a consequence of complex interactions between multiple loci. Analysis of additional life history traits indicates that the QTL regions identified in these screens are associated with effects on other traits such as lifespan and reproduction, suggesting that the incompatibilities are likely to be deleterious. Taken together, these results indicate that numerous BDM incompatibilities that could contribute to reproductive isolation can be detected and mapped within C. elegans.
|Prediction of tomato seedling morphology by X-ray analysis of seeds
Burg, W.J. van der; Aartse, J.W. ; Zwol, R.A. van; Jalink, H. ; Bino, R.J. - \ 1995
Journal of the American Society for Horticultural Science 119 (1995). - ISSN 0003-1062 - p. 258 - 263.
Non-destructive seed evaluation with impact measurements and X-ray analysis
Burg, W.J. van der; Jalink, H. ; Zwol, R.A. van; Aartse, J.W. ; Bino, R.J. - \ 1995
Acta Horticulturae 362 (1995). - ISSN 0567-7572 - p. 149 - 157.
Non-destructive testing is important in the search for seed characteristics that relate to quality. It provides a means for consecutive testing on a seed by seed basis. If the tests are fast and can be automated they can form the basis for rapid new analysis methods or online sorting. X-ray analysis of tomato seeds enables us to predict seedling morphology. Acoustic analysis provides rapid physical assessment of seeds, which correlates with seed coat conditions. Both techniques can be automated and provide potential new sorting techniques. The X-ray technique provided us with some evidence for the origin of vigour differences in tomato seeds: morphological differences in seed embryos and the availability of endosperm proved to be crucial.
Non-destructive evaluation of internal quality of radish tubers using specific gravity, X-ray analysis and near infrared spectroscopy
Marcelis, L.F.M. ; Burg, W.J. van der; Frankhuizen, R. ; Verkerke, D.R. ; Aartse, J.W. ; Boekestein, A. - \ 1995
Acta Horticulturae 379 (1995). - ISSN 0567-7572 - p. 527 - 535.
One of the main determinants of the internal quality of radish tubers (Raphanus sativus L. var. radicula Pers.) is sponginess. Sponginess is a physiological disorder that is characterized by dead cells in which gas embolism is formed. Eventually this may lead to totally hollow tubers. Up to now the degree of sponginess has been determined by visually comparing the transversely cut tuber with a series of photographs of spongy tubers. This method is subjective, destructive and time-consuming. Therefore, an objective, non-destructive and rapid method is desired to determine the degree of sponginess. In this preliminary study some of these methods were tested, including (1) measurement of specific gravity, (2) X-ray analysis and (3) near infrared spectroscopy. Two batches of 50 tubers were analysed by all three methods. Finally, the degree of sponginess was determined visually by comparing the transversely cut tubers with a standard series of photographs. The visually estimated relative area of spongy tissue was highly correlated with specific gravity (R=-0.92) as well as X-ray analysis (R=0.87) and correlated reasonably well with near infrared reflectance (R=0.73) and transmittance (R=0.66). When sponginess led to hollowness of the tubers the specific gravity was reduced, but the X-ray technique did not discriminate between spongy tissue with and spongy tissue without hollows, while the near infrared spectroscopy tended to predict a lower degree of sponginess when the spongy tissue showed hollows. Advantages and disadvantages of each method are discussed
|Quality determination of seed by X-ray testing and computerized image analysis
Polder, G. ; Heijden, G.W.A.M. van der; Burg, W.J. van der; Aartse, J.W. - \ 1994
In: The proceedings of the 5th international conference on signal processing applications and technology, Dallas, TX, USA, 18-21 October 1994 Dallas : ICSPAT - p. 1523 - 1528.
|Photoacoustic detection of temperature phenomena in beeswax.
Jalink, H. ; Bicanic, D. ; Burg, J. van der; Zwol, R.A. van; Aartse, J.W. - \ 1992
In: Photoacoustic and photothermal phenomena 3 / Bicanic, D., Heidelberg/Berlin : Springer Verlag (Springer Series in Optical Sciences 69) - p. 586 - 590.