Animal Botulism Outcomes in the AniBioThreat Project. Biosecur. Bioterror
Woudstra, C. ; Tevell Aberg, A. ; Skarin, H. ; Anniballi, F. ; Medici, D. De; Bano, L. ; Koene, M.G.J. ; Löfström, Ch. ; Hansen, T. ; Hedeland, M. ; Fach, P. - \ 2013
Biosecurity and Bioterrorism: biodefense strategy, practice and science 11 (2013)Suppl. 1. - ISSN 1538-7135 - p. S177 - S182.
real-time pcr - polymerase-chain-reaction - neurotoxin-producing clostridia - mass-spectrometry - quantitative detection - bovine samples - wound botulism - sybr green - group-iii - types c
Botulism disease in both humans and animals is a worldwide concern. Botulinum neurotoxins produced by Clostridium botulinum and other Clostridium species are the most potent biological substances known and are responsible for flaccid paralysis leading to a high mortality rate. Clostridium botulinum and botulinum neurotoxins are considered potential weapons for bioterrorism and have been included in the Australia Group List of Biological Agents. In 2010 the European Commission (DG Justice, Freedom and Security) funded a 3-year project named AniBioThreat to improve the EU's capacity to counter animal bioterrorism threats. A detection portfolio with screening methods for botulism agents and incidents was needed to improve tracking and tracing of accidental and deliberate contamination of the feed and food chain with botulinum neurotoxins and other Clostridia. The complexity of this threat required acquiring new genetic information to better understand the diversity of these Clostridia and develop detection methods targeting both highly specific genetic markers of these Clostridia and the neurotoxins they are able to produce. Several European institutes participating in the AniBioThreat project collaborated on this program to achieve these objectives. Their scientific developments are discussed here.
The Workshop on Animal Botulism in Europe
Skarin, H. ; Tevell Aberg, A. ; Woudstra, C. ; Hansen, T. ; Löfström, Ch. ; Koene, M.G.J. ; Bano, L. ; Hedeland, M. ; Anniballi, F. ; Medici, D. De; Olsson Engvall, E. - \ 2013
Biosecurity and Bioterrorism: biodefense strategy, practice and science 11 (2013)S1. - ISSN 1538-7135 - p. S183 - S190.
clostridium-botulinum - endopep-ms - neurotoxins - cattle - mouse
A workshop on animal botulism was held in Uppsala, Sweden, in June 2012. Its purpose was to explore the current status of the disease in Europe by gathering the European experts in animal botulism and to raise awareness of the disease among veterinarians and others involved in biopreparedness. Animal botulism is underreported and underdiagnosed, but an increasing number of reports, as well as the information gathered from this workshop, show that it is an emerging problem in Europe. The workshop was divided into 4 sessions: animal botulism in Europe, the bacteria behind the disease, detection and diagnostics, and European collaboration and surveillance. An electronic survey was conducted before the workshop to identify the 3 most needed discussion points, which were: prevention, preparedness and outbreak response; detection and diagnostics; and European collaboration and surveillance. The main conclusions drawn from these discussions were that there is an urgent need to replace the mouse bioassay for botulinum toxin detection with an in vitro test and that there is a need for a European network to function as a reference laboratory, which could also organize a European supply of botulinum antitoxin and vaccines. The foundation of such a network was discussed, and the proposals are presented here along with the outcome of discussions and a summary of the workshop itself.
|European Allergy White Paper Update
Aberg, N. ; Berlin, A. ; Bertollini, R. ; Bonini, S. ; Brunekreef, B. ; Carlsen, K.H. ; Weck, A. de - \ 1999
In: European Allergy Update / Wahn, U., Werner, J.A., de Weck, A., Holgate, S.T., Hejdenberg, K., UCB Institute of Allergy - p. 1 - 57.