Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Visual Detection of Potato leafroll virus by One-step Reverse Transcription Loop-Mediated Isothermal Amplification of DNA with Hydroxynaphthol Blue Dye
    Ahmadi, S. ; Almasi, A.M. ; Fatehi, F. ; Struik, P.C. ; Moradi, A. - \ 2013
    Journal of Phytopathology 161 (2013). - ISSN 0931-1785 - p. 120 - 124.
    nucleic-acid amplification - rapid detection - rt-lamp - assay - tubers - spp. - gene
    Loop-mediated isothermal amplification (LAMP) assay is a novel technique for amplifying DNA under constant temperature, with high specificity, sensitivity, rapidity and efficiency. We applied reverse transcription loop-mediated isothermal amplification (RT-LAMP) to visually detect Potato leafroll virus. One-step RT-LAMP was performed using RNA of PLRV-infected potato leaves and a set of primers (F3, B3, FIP, BIP, LF and LB) designed for RT-LAMP reaction of the coat protein (CP) gene of PLRV. Positive effects of RT-LAMP were detected by agarose gel electrophoresis and hydroxynaphthol blue (HNB) dye and were shown by a colour change from violet to sky blue. RT-LAMP with HNB dye proved to be a simple assay for the rapid detection of PLRV.
    A survey of the apes in the Dzanga-Ndoki National Park, Central African Republic: A comparison between the census and survey methods of estimating the gorilla (Gorilla gorilla gorilla) and chimpanzee (Pan troglodytes) nest group density
    Almasi, A. ; Blom, A. ; Heitkönig, I.M.A. ; Kpanou, J.B. ; Prins, H.H.T. - \ 2001
    African Journal of Ecology 39 (2001). - ISSN 0141-6707 - p. 98 - 105.
    A survey of apes was carried out between October 1996 and May 1997 in the Dzanga sector of the Dzanga-Ndoki National Park, Central African Republic (CAR), to estimate gorilla (Gorilla gorilla gorilla) and chimpanzee (Pan troglodytes) densities. The density estimates were based on nest counts. The strip transect census and the line transect survey method (Standing Crop Nest Count) were used to estimate the gorilla nest group density. The strip transect has been most commonly used to date. It assumes that all nest groups within the width of the strip are detected, but as this assumption is easily violated in the dense tropical rain forest, the line transect survey was also used. In this method, only the nest groups on the transect line itself should be detected. This method proved to be an adequate and easy technique for estimating animal densities in dense vegetation. The gorilla density of 1.6 individuals km2 (line transect survey method) found for the Dzanga sector is one of the highest densities ever reported in the literature for the Western lowland gorilla. The density estimate for chimpanzees was 0.16 individuals km2 (census method). The results of this study confirm the importance of the Dzanga-Ndoki National Park for primate conservation.
    A survey of the diurnal primates in the Dzanga-Ndoki National Park, Central African Republic
    Blom, A. ; Almasi, A. ; Heitkonig, I.M.A. ; Kpanou, J.B. ; Prins, H.H.T. - \ 2000
    African Journal of Ecology 2000 (2000). - ISSN 0141-6707
    The Mongambe Research Camp, Dzanga-Ndoki National Park, Central African Republic
    Almasi, A. ; Blom, A. ; Prins, H.H.T. - \ 1999
    Gorilla Conservation News 13 (1999). - p. 5 - 6.
    The Mongambe Research Camp, Dzanga-Ndoki National Park, Central African Republic (C.A.R.)
    Almasi, A. ; Blom, A. ; Prins, H.H.T. - \ 1999
    Gorilla Conservation News 13 (1999). - p. 5 - 5.
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