Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Pre-clinical protein screening in bioengineered intestinal tubules
    Jochemsen, P. ; Garssen, Johan ; Rietveld, P. ; Ariens, R.M.C. ; Bastiaan-Net, S. ; Wichers, H.J. ; Bergenhenegouwen, Jeroen van; Masereeuw, Rosalinde - \ 2019
    - 1 p.
    Development of a trained immunity and resilience model for testing of orally applied β-glucans
    Moerings, Bart ; Graaff, Priscilla de; Wichers, H.J. ; Garssen, Johan ; Witkamp, R.F. ; Debets, R. ; Mes, J.J. ; Bergenhenegouwen, Jeroen van; Govers, C.C.F.M. - \ 2019
    Development and validation of bioengineered intestinal tubules for translational research aimed at safety and efficacy testing of drugs and nutrients
    Jochems, Paulus G.M. ; Bergenhenegouwen, Jeroen van; Genderen, Anne Metje van; Eis, Sophie T. ; Wilod Versprille, Livia J.F. ; Wichers, Harry J. ; Jeurink, Prescilla V. ; Garssen, Johan ; Masereeuw, Rosalinde - \ 2019
    Toxicology in Vitro 60 (2019). - ISSN 0887-2333 - p. 1 - 11.
    Caco-2 - In vitro - Microfluidic and screening - Small intestine

    Currently used intestinal cell models have limited translational value, therefore, development of novel in vitro intestinal models that recapitulate the human in vivo setting more closely are of interest. Here, an advanced intestinal model was developed by the incorporation of physiological parameters, such as extracellular matrix (ECM)elements and shear stress, to cultured Caco-2 cells in a 3-dimensional environment. Caco-2 cells grown on ECM-coated hollow fiber membranes (HFM)under physiological shear stress show an improved phenotype, as demonstrated by the presence of enterocytes, goblet, Paneth, enteroendocrine and stem cells. Additionally, this model showed signs of an improved morphology due to the appearance of villi-like structures. Similar to epithelial cells grown on Transwells™, the current model remains easy to use, cost efficient and allows apical and basolateral access. The bioengineered intestinal tubule was validated by exposure to Clostridium difficile toxin A, the leading cause of healthcare-associated diarrhea. The loss of the tight junction network was supported by an increase in inulin-FITC leakage and the number of goblet cells increased, in agreement with clinical findings. In addition to toxicity screening, the bioengineered intestinal tubules are considered useful for drug and nutrient safety and efficacy testing.

    Mice co-administrated with partially hydrolysed whey proteins and prebiotic fibre mixtures show allergen-specific tolerance and a modulated gut microbiota
    Kleinjans, L. ; Veening-Griffioen, D.H. ; Wehkamp, T. ; Bergenhenegouwen, J. van; Knol, J. ; Garssen, J. ; Knippels, L.M.J. ; Belzer, C. ; Jeurink, P.V. - \ 2019
    Beneficial Microbes 10 (2019)2. - ISSN 1876-2883 - p. 165 - 178.
    cow’s milk allergy - microbiota - non-digestible oligosaccharides - preventive tolerance induction

    Non-breastfed infants at-risk of allergy are recommended to use a hydrolysed formula before the age of 6 months. The addition of prebiotics to this formula may reduce the allergy development in these infants, but clinical evidence is still inconclusive. This study evaluates (1) whether the exposure duration to different prebiotics alongside a partially hydrolysed whey protein (pHP) influences its' effectiveness to prevent allergy development and (2) whether the gut microbiota plays a role in this process. Mice orally sensitised with whey and/or cholera toxin were orally treated for six days before sensitization with phosphate buffered saline, whey or pHP to potentially induce tolerance. Two groups received an oligosaccharide diet only from day -7 until -2 (GFshort and GFAshort) whereas two other groups received their diets from day -15 until 37 (GFlong and GFAlong). On day 35, mice underwent an intradermal whey challenge, and the acute allergic skin response, shock score, and body temperatures were measured. At day 37, mice received whey orally and serum mouse mast cell protease-1, SLPI and whey-specific antibodies were assessed. Faecal samples were taken at day -15, -8 and 34. Feeding mice pHP alone during tolerance induction did not reduce ear swelling. The tolerance inducing mechanisms seem to vary according to the oligosaccharide-composition. GFshort, GFlong, and GFAlong reduced the allergic skin response, whereas GFAshort was not potent enough. However, in the treatment groups, the dominant Lactobacillus species decreased, being replaced by Bacteroidales family S24-7 members. In addition, the relative abundance of Prevotella was significantly higher in the GFlong, GFAshort and GFAlong groups. Co-administration of oligosaccharides and pHP can induce immunological tolerance in mice, although tolerance induction was strongest in the animals that were fed oligosaccharides during the entire protocol. Some microbial changes coincided with tolerance induction, however, a specific mechanism could not be determined based on these data.

    Sex differences in lipid metabolism are affected by presence of the gut microbiota
    Baars, Annemarie ; Oosting, Annemarie ; Lohuis, Mirjam ; Koehorst, Martijn ; Aidy, Sahar El; Hugenholtz, Floor ; Smidt, Hauke ; Mischke, Mona ; Boekschoten, Mark V. ; Verkade, Henkjan J. ; Garssen, Johan ; Beek, Eline M. van der; Knol, Jan ; Vos, Paul de; Bergenhenegouwen, Jeroen van; Fransen, Floris - \ 2018
    Scientific Reports 8 (2018)1. - ISSN 2045-2322

    Physiological processes are differentially regulated between men and women. Sex and gut microbiota have each been demonstrated to regulate host metabolism, but it is unclear whether both factors are interdependent. Here, we determined to what extent sex-specific differences in lipid metabolism are modulated via the gut microbiota. While male and female Conv mice showed predominantly differential expression in gene sets related to lipid metabolism, GF mice showed differences in gene sets linked to gut health and inflammatory responses. This suggests that presence of the gut microbiota is important in sex-specific regulation of lipid metabolism. Further, we explored the role of bile acids as mediators in the cross-talk between the microbiome and host lipid metabolism. Females showed higher total and primary serum bile acids levels, independent of presence of microbiota. However, in presence of microbiota we observed higher secondary serum bile acid levels in females compared to males. Analysis of microbiota composition displayed sex-specific differences in Conv mice. Therefore, our data suggests that bile acids possibly play a role in the crosstalk between the microbiome and sex-specific regulation of lipid metabolism. In conclusion, our data shows that presence of the gut microbiota contributes to sex differences in lipid metabolism.

    Human milk: a source of more life than we imagine
    Jeurink, P.V. ; Bergenhenegouwen, J. van; Jimenez, E. ; Knippels, L.M.J. ; Fernandez, L. ; Garssen, J. ; Knol, J. ; Rodriguez, J.M. ; Martin, R. - \ 2013
    Beneficial Microbes 4 (2013)1. - ISSN 1876-2883 - p. 17 - 30.
    lactic-acid bacteria - human breast-milk - fragment-length-polymorphism - human skin microbiome - healthy women - infectious mastitis - dendritic cells - infant gut - staphylococcus-epidermidis - intestinal microbiota
    The presence of bacteria in human milk has been acknowledged since the seventies. For a long time, microbiological analysis of human milk was only performed in case of infections and therefore the presence of non-pathogenic bacteria was yet unknown. During the last decades, the use of more sophisticated culture-dependent and -independent techniques, and the steady development of the -omic approaches are opening up the new concept of the 'milk microbiome', a complex ecosystem with a greater diversity than previously anticipated. In this review, possible mechanisms by which bacteria can reach the mammary gland (contamination versus active migration) are discussed. In addition, the potential roles of human milk for both infant and maternal health are summarised. A better understanding of the link between the milk microbiome and health benefit, the potential factors influencing this relationship and whether or not it can be influenced by nutrition is required to open new avenues in the field of pregnancy and lactation.
    Dose-dependent effects of leucine supplementation on preservation of muscle mass in cancer cachectic mice
    Peters, S.J. ; Helvoort, A. van; Kegler, D. ; Argiles, J.M. ; Luiking, Y.C. ; Laviano, A. ; Bergenhenegouwen, J. van; Deutz, N.E.P. ; Haagsman, H.P. ; Gorselink, M. ; Norren, K. van - \ 2011
    Oncology Reports : an international journal devoted to fundamental and applied research in oncology 26 (2011)1. - ISSN 1021-335X - p. 247 - 254.
    chain amino-acids - urinary 3-methylhistidine excretion - tumor-bearing rats - skeletal-muscle - protein-synthesis - pancreatic-cancer - weight-loss - fish-oil - in-vivo - colon-26 adenocarcinoma
    Cancer cachexia, which is characterized by muscle wasting, is associated with increased morbidity and mortality. Because muscle protein synthesis may be increased and protein breakdown reduced by leucine supplementation, we used the C26 tumor-bearing cachectic mouse model to assess the effects of dietary supplementation with leucine on muscle weight and the markers of muscle protein breakdown (mRNA of atrogin and murf). Male CD2F1 mice were subcutaneously inoculated with tumor cells (tumor-bearing mice; TB) or were sham injected (control; C). They were fed standard diets or diets supplemented with leucine [1 gr (TB1Leu) or 8 gr (TB8Leu) supplemented leucine per kg feed]; TB and C received 8.7% Leu/g protein, TB1Leu received 9.6% Leu/g protein and TB8Leu received 14.6 Leu/g protein. After 21 days, the following were determined: body weights, plasma amino-acid concentrations, tumor size and muscle mass of the gastrocnemius (mG), tibialis anterior (mTA), extensor digitorum longus (mEDL) and soleus (mS) muscles. In tumor-bearing (TB) mice, carcass and skeletal muscle masses decreased, and levels of atrogin and murf mRNA in the mEDL increased. Muscle-mass loss was counteracted dose-dependently by leucine supplementation: relative to TB, the mass of the mG was +23% in TB8Leu, and +22% in mTA (p
    Paprikateelt onder glas
    Bergenhenegouwen, L.A.M. ; Goes, A.G.T.M. ; Groenewegen, J.H. - \ 1983
    Naaldwijk : Proefstation voor Tuinbouw onder Glas [etc.] (Informatiereeks / Proefstation voor Tuinbouw onder Glas te Naaldwijk en Consulentschappen voor de Tuinbouw te Naaldwijk 5)
    capsicum annuum - nederland - paprika - glastuinbouw - capsicum annuum - netherlands - sweet peppers - greenhouse horticulture
    Onderzoek naar enkele aspecten van potgrond bij sla
    Esch, H.G.A. ; Bergenhenegouwen, J. van - \ 1973
    Naaldwijk : Proefstation voor de Groenten- en Fruitteelt onder Glas - 3
    Optreden en verspreiden van Botrytis cinerea in tomategewassen, groeiend bij verschillende bemestingsnibeaus in twee onverwarmde warenhuizen te Maasland
    Verhoeff, K. ; Vlaster, T. ; Bergenhenegouwen, J.P. van - \ 1968
    Naaldwijk : Proefstation voor de Groenten- en Fruitteelt onder Glas - 3
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