Comparison of Small Gut and Whole Gut Microbiota of First-Degree Relatives With Adult Celiac Disease Patients and Controls
Bodkhe, Rahul ; Shetty, Sudarshan A. ; Dhotre, Dhiraj P. ; Verma, Anil K. ; Bhatia, Khushbo ; Mishra, Asha ; Kaur, Gurvinder ; Pande, Pranav ; Bangarusamy, Dhinoth K. ; Santosh, Beena P. ; Perumal, Rajadurai C. ; Ahuja, Vineet ; Shouche, Yogesh S. ; Makharia, Govind K. - \ 2019
Frontiers in Microbiology 10 (2019). - ISSN 1664-302X
Recent studies on celiac disease (CeD) have reported alterations in the gut microbiome. Whether this alteration in the microbial community is the cause or effect of the disease is not well understood, especially in adult onset of disease. The first-degree relatives (FDRs) of CeD patients may provide an opportunity to study gut microbiome in pre-disease state as FDRs are genetically susceptible to CeD. By using 16S rRNA gene sequencing, we observed that ecosystem level diversity measures were not significantly different between the disease condition (CeD), pre-disease (FDR) and control subjects. However, differences were observed at the level of amplicon sequence variant (ASV), suggesting alterations in specific ASVs between pre-disease and diseased condition. Duodenal biopsies showed higher differences in ASVs compared to fecal samples indicating larger disruption of the microbiota at the disease site. The duodenal microbiota of FDR was characterized by significant abundance of ASVs belonging to Parvimonas, Granulicatella, Gemella, Bifidobacterium, Anaerostipes, and Actinomyces genera. The duodenal microbiota of CeD was characterized by higher abundance of ASVs from genera Megasphaera and Helicobacter compared to the FDR microbiota. The CeD and FDR fecal microbiota had reduced abundance of ASVs classified as Akkermansia and Dorea when compared to control group microbiota. In addition, predicted functional metagenome showed reduced ability of gluten degradation by CeD fecal microbiota in comparison to FDRs and controls. The findings of the present study demonstrate differences in ASVs and predicts reduced ability of CeD fecal microbiota to degrade gluten compared to the FDR fecal microbiota. Further research is required to investigate the strain level and active functional profiles of FDR and CeD microbiota to better understand the role of gut microbiome in pathophysiology of CeD.
|Leadership and Turnover: The Contradicitons of Irrigation Management Reform in the People's Republic of China
Mollinga, P.P. ; Hong, G. ; Bhatia, A.M. - \ 2005
In: Asian Irrigation in Transition. Responding to Challenges / Shivakoti, G.P, Vermillion, D.L., Lam, Wai-Fung, Ostrom, E., Pradhan, U., Yoder, R., New Delhi : SAGE Publications - ISBN 9780761933502 - p. 310 - 345.
|Risk and control of Enterobacter sakazakii
Nazarowec-White, M. ; Forsythe, S. ; Smoot, L. ; Bhatia, J. ; Zwietering, M.H. ; Zink, D. - \ 2004
In: 91st Annual Meeting IAFP 2004: Program and Abstract Book Advanced Food Saftey Worldwide, Phoenix, Arizona, 8-11 August 2004. - International Association for Food Protection - p. 164 - 164.
|Irrigation reform in the people's republic of China. Institutional transformation in Shijin, Jingxing Mianyou and Zaohe irrigation systems
Bhatia, A.M. ; Hong, G. ; Mollinga, P.P. - \ 2003
Wageningen : Irrigation and Water Engineering Group (CWP Research Papers 13) - 52 p.
|Institutional innovation in donorfunded land and water development project implementation : case studies from Uttar Pradesh and West Bengal, India
Bhatia, A.M. - \ 2002
Wageningen : Irrigation and Water Engineering Group (CWP Research Papers 5) - 78 p.
|A preliminary study using the cumulative gas production technique to compare the kinetics of different fermentations by use of standard substrates.
Williams, B.A. ; Bhatia, S.K. ; Boer, H. ; Tamminga, S. - \ 1995
Annales de Zootechnie 44 (1995)Suppl. 1. - ISSN 0003-424X - p. 35 - 35.
|Final report on Marie Curie Fellowship funded by the Commission of European Communities.
Bhatia, S.K. - \ 1994
Unknown Publisher - 18 p.