Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

    Records 1 - 17 / 17

    • help
    • print

      Print search results

    • export

      Export search results

    Check title to add to marked list
    Training of primary chicken monocytes results in enhanced pro-inflammatory responses
    Verwoolde, Michel B. ; Biggelaar, Robin H.G.A. van den; Baal, Jürgen van; Jansen, Christine A. ; Lammers, Aart - \ 2020
    Veterinary Sciences 7 (2020)3. - ISSN 2306-7381
    Flow cytometry - Inflammatory response - Innate immune memory - Macrophages - Primary chicken monocytes - Β-glucan

    Beta-glucan-stimulated mammalian myeloid cells, such as macrophages, show an increased responsiveness to secondary stimulation in a nonspecific manner. This phenomenon is known as trained innate immunity and is important to prevent reinfections. Trained innate immunity seems to be an evolutionary conserved phenomenon among plants, invertebrates and mammalian species. Our study aimed to explore the training of primary chicken monocytes. We hypothesized that primary chicken monocytes, similar to their mammalian counterparts, can be trained with β-glucan resulting in increased responses of these cells to a secondary stimulus. Primary blood monocytes of white leghorn chickens were primary stimulated with β-glucan microparticulates (M-βG), lipopolysaccharide (LPS), recombinant chicken interleukin-4 (IL-4) or combinations of these components for 48 h. On day 6, the primary stimulated cells were secondary stimulated with LPS. Nitric oxide (NO) production levels were measured as an indicator of pro-inflammatory activity. In addition, the cells were analyzed by flow cytometry to characterize the population of trained cells and to investigate the expression of surface markers associated with activation. After the secondary LPS stimulation, surface expression of colony stimulating factor 1 receptor (CSF1R) and the activation markers CD40 and major histocompatibility complex class II (MHC-II) was higher on macrophages that were trained with a combination of M-βG and IL-4 compared to unstimulated cells. This increased expression was paralleled by enhanced NO production. In conclusion, this study showed that trained innate immunity can be induced in primary chicken monocytes with β-glucan, which is in line with previous experiments in mammalian species. Innate immune training may have the potential to improve health and vaccination strategies within the poultry sector.

    Innate immune training and metabolic reprogramming in primary monocytes of broiler and laying hens
    Verwoolde, Michel B. ; Biggelaar, Robin H.G.A. van den; Vries Reilingh, Ger de; Arts, Joop A.J. ; Baal, Jürgen van; Lammers, Aart ; Jansen, Christine A. - \ 2020
    Developmental and Comparative Immunology 114 (2020). - ISSN 0145-305X
    Chickens - Immune training - Lipopolysaccharide - Metabolism - Monocytes - β-glucan

    Recently, we have reported trained innate immunity in laying chicken monocytes. In the present study, we further investigated trained innate immunity of monocytes in layers and broilers. Monocytes of both breeds isolated from blood were trained in vitro with β-glucan, rec-chicken IL-4 or a combination of both, and restimulated with lipopolysaccharide (LPS), after which inflammation and metabolism-related responses were measured. Training of laying and broiler hen monocytes resulted in increased mRNA levels of IL-1β, iNOS and HIF-1α, but enhanced surface expression of CD40 and NO production was only observed in layers. Our in vitro study demonstrates that monocytes from different genetic backgrounds can be trained. However, the observed differences suggest a differential effect on immune functionality associated with innate training. Whether these differences in immune functions between layers and broilers have effect on disease resistance remains to be elucidated.

    Grote vraagtekens bij drijfmest in rij
    Kroonen-Backbier, Brigitte - \ 2020
    The Maastricht-FFQ: Development and Validation of a Comprehensive Food Frequency Questionnaire for The Maastricht Study
    Dongen, Martien C.J.M. van; Wijckmans-Duysens, Nicole E.G. ; Biggelaar, Louise J.C.J. den; Ock, Marga C. ; Meijboom, S. ; Brants, H.A.M. ; Vries, J.H.M. de; Feskens, E.J.M. ; Bueno-de-Mesquita, H.B. ; Geelen, M.M.E.E. ; Stehouwer, C.D.A. ; Dagnelie, Pieter C. ; Eussen, Simone J.P.M. - \ 2019
    Nutrition 62 (2019). - ISSN 0899-9007
    Objective: To develop and validate a comprehensive food frequency questionnaire (FFQ) for The Maastricht Study, a population-based prospective cohort study in Maastricht, The Netherlands. Research Methods & Procedures: Item selection for the FFQ was based on explained variation and contribution to intake of energy and 24 nutrients. For validation, the FFQ was filled out by 135 participants (25-70 years) of the Nutrition Questionnaires plus study. Per person, on average 2.8 (range 1 to 5) telephone-based 24h dietary recalls (24hRs), two 24h urinary samples and one blood sample were available. Validity of 54 nutrients and 22 food groups was assessed by ranking agreement, correlation coefficients, attenuation factors, and ultimately de-attenuated correlation coefficients (validity coefficients). Results: Median correlation coefficients for energy and macronutrients, micronutrients and food groups were 0.45, 0.36 and 0.38, respectively. Median de-attenuated correlation coefficients were 0.53 for energy and macronutrients, 0.45 for micronutrients and 0.64 for food groups, being >0.50 for 18 out of 22 macronutrients and 16 out of 30 micronutrients, and >0.50 for 17 out of 22 food groups. The FFQ underestimated protein and potassium intake compared to 24h urinary nitrogen and potassium excretion by -18% and -2%, respectively. Correlation coefficients ranged from 0.50 and 0.55 for (fatty) fish intake and plasma EPA and DHA, and from 0.26-0.42 between fruit and vegetable intake and plasma carotenoids. Conclusion: Overall, the validity of the 253-item Maastricht-FFQ is good. The comprehensiveness of this FFQ make it well-suited for use in The Maastricht Study and similar populations.
    The influence of genetic background on trained innate immunity in chicken macrophages
    Verwoolde, M.B. ; Biggelaar, Robin H.G.A. van den; Baal, J. van; Jansen, Christine A. ; Lammers, A. - \ 2019
    Living Lab om Peel-problemen te tackelen
    Kroonen-Backbier, B.M.A. - \ 2019
    Trained innate immunity in chicken macrophages
    Verwoolde, M.B. ; Biggelaar, Robin H.G.A. van den; Baal, J. van; Jansen, Christine A. ; Lammers, A. - \ 2019
    In: Trade-offs in science – keeping the Balance. - Wageningen University & Research - p. 27 - 27.
    High-resolution optical dating of Late Holocene storm surge deposits – a showcase from Schokland (Noordoostpolder, the Netherlands)
    Biggelaar, D.F.A.M. van den; Wallinga, J. ; Balen, R.T. van; Kasse, C. ; Troelstra, S. ; Kluiving, S.J. - \ 2019
    Earth Surface Processes and Landforms 44 (2019)4. - ISSN 0197-9337 - p. 886 - 899.
    Storm surges have a major impact on land use and human habitation in coastal regions. Our knowledge of this impact can be improved by correlating long-term historical storm records with sedimentary evidence of storm surges, but so far few studies have applied such an approach. Here we apply, for the first time, state-of-the-art optically stimulating luminescence (OSL) methods to obtain high-resolution age information on a sequence of Late Holocene storm surge deposits. By combining this chronological framework of storm surges with other reconstruction methods, we investigate the storm surge impact on the former island Schokland, located in a former inlet of the North Sea (central Netherlands). During the Late Holocene, Schokland transformed from a peat area that gradually inundated (~800 CE) via an island in a marginal marine environment (~1600 CE) to a land-locked island in the reclaimed Province of Flevoland (1942 CE). These transitions are recorded in the sediment archive of the island, consisting of silty clay with sandy intervals deposited during storm surges. A series of ten quartz OSL ages, obtained using best-practice methods to deal with incomplete resetting of the OSL signal and dose rate heterogeneity, reveal two periods of storm surge deposition, around 1600 CE and between 1742 and 1822 CE. Historical sources indicate that major storm surges hit Schokland during these periods. Laboratory analyses (thermogravimetry, grain-size, foraminifera, bivalves and ostracods) corroborates the existence of the two sets of storm surge deposits within the clay sequence. Our study sets a benchmark for obtaining robust depositional age constraints from storm surge sediments, and demonstrates the great potential of modern OSL methods to contribute to improved assessment of storm surge risk. © 2018 John Wiley & Sons, Ltd.
    In vitro model to study trained innate immunity in chicken primary monocytes
    Verwoolde, M.B. ; Biggelaar, Robin H.G.A. van den; Baal, J. van; Jansen, Christine A. ; Lammers, A. - \ 2018
    Stevigheid maïs steeds belangrijker
    Groten, J.A.M. - \ 2017
    Stevigheid maïs steeds belangrijker
    Groten, J.A.M. - \ 2017
    Stevigheid maïs steeds belangrijker
    Groten, J.A.M. - \ 2017
    Stevigheid maïs steeds belangrijker
    Groten, J.A.M. - \ 2017
    Dairycampus test droogzet-app
    Hoeij, R.J. van; Knegsel, A.T.M. van - \ 2017
    Droogstand - Dairy Campus - droogzet-app

    Het project ‘Droogstand op maat’ startte recent met het testen van een droogzet-app. Met behulp van die app kan een melkveehouder in de toekomst bepalen welke droogstandslengte voor zijn individuele koeien het beste is (0, 30 of 60 dagen) en of hij wel of niet een droogzetter met antibiotica dient te gebruiken.

    Sensitivities of baseline isolates and boscalid-resistant mutants of Alternaria alternata from pistachio to fluopyram, penthiopyrad, and fluxapyroxad
    Avenot, H.F. ; Biggelaar, H. van den; Morgan, D.P. ; Moral, J. ; Joosten, M.H.A.J. ; Michailides, T.J. - \ 2014
    Plant Disease 98 (2014)2. - ISSN 0191-2917 - p. 197 - 205.
    molecular characterization - botrytis-cinerea - inhibiting fungicides - california pistachio - didymella-bryoniae - sdhi fungicides - late blight - mutations - azoxystrobin - populations
    Resistance of Alternaria alternata to boscalid, the first succinate dehydrogenase inhibitor (SDHI) fungicide labeled on pistachio, has become a common occurrence in California pistachio orchards and affects the performance of this fungicide. In this study, we established the baseline sensitivities of A. alternata to the new SDHIs fluopyram, fluxapyroxad, and penthiopyrad and assessed their cross resistance patterns with boscalid. Examination of the effective fungicide concentration that inhibits mycelial growth to 50% relative to the control (EC50) for 50 baseline isolates revealed that the majority were sensitive to boscalid, penthiopyrad, fluopyram, and fluxapyroxad. Analysis of EC50 values for boscalid for 117 A. alternata isolates originating from boscalid-exposed orchards showed that 44, 3, 1, and 69 isolates had sensitive, reduced sensitivity, moderately resistant, and highly resistant boscalid phenotypes, respectively. Molecular investigation of the occurrence of known SDH mutations showed that, among the 69 isolates highly resistant to boscalid, 44, 2, 14, and 1 isolates possessed the mutations leading to the H277Y, H277R, H134R, and H133R amino acid substitutions in AaSDHB, AaSDHB, AaSDHC, and AaSDHD subunits, respectively. Some SDHB or SDHC mutants displayed highly sensitive, sensitive, or reduced sensitivity phenotypes toward penthiopyrad or fluxapyroxad, whereas other had low, moderate, or high levels of resistance to these fungicides. In contrast, all the SDHB mutants were sensitive to fluopyram, while 10, 5, and 1 SDHC mutants had sensitive, reduced sensitivity, and moderately resistant fluopyram phenotypes, respectively. The SDHD mutant had reduced sensitivity to fluopyram and penthiopyrad but was highly resistant to fluxapyroxad. The discrepancies of cross-resistance patterns between SDHIs suggest that their binding sites in complex II may differ slightly and that additional mechanisms of resistance to these compounds are likely involved. Ultimately, the findings of this study should lead to the rational and sustained deployment of new SDHIs in Alternaria late blight spray programs.
    Occurrence and survival of verocytotoxin-producing Escherichia coli in raw cow's milk in The Netherlands.
    Heuvelink, A.E. ; Bleumink, B. ; Biggelaar, F.L.A.M. van den; Giffel, M.C. te; Beumer, R.R. ; Boer, E. de - \ 1998
    Journal of Food Protection 61 (1998). - ISSN 0362-028X - p. 1597 - 1601.
    Kanttekeningen bij de beschouwingen van Van den Biggelaar over het consumptiebeleid in 'Naar een konsumptiebeleid'
    Meulenberg, M.T.G. - \ 1978
    Economische Notities / Wiardi Beckman Stichting 4 (1978). - p. 73 - 85.
    Check title to add to marked list

    Show 20 50 100 records per page

    Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.