Proteomic analysis of the plasma membrane-movement tubule complex of cowpea mosaic virus
Hollander, P.W. Den; Sousa Geraldino Duarte, Priscilla de; Bloksma, Hanke ; Boeren, Sjef ; Lent, J.W.M. van - \ 2016
Archives of Virology (2016). - ISSN 0304-8608 - p. 1309 - 1314.
Cowpea mosaic virus forms tubules constructed from the movement protein (MP) in plasmodesmata (PD) to achieve cell-to-cell movement of its virions. Similar tubules, delineated by the plasma membrane (PM), are formed protruding from the surface of infected protoplasts. These PM-tubule complexes were isolated from protoplasts by immunoprecipitation and analysed for their protein content by tandem mass spectrometry to identify host proteins with affinity for the movement tubule. Seven host proteins were abundantly present in the PM-tubule complex, including molecular chaperonins and an AAA protein. Members of both protein families have been implicated in establishment of systemic infection. The potential role of these proteins in tubule-guided cell-cell transport is discussed.
Organic Milk Quality in the Netherlands : Distinguishable from conventional milk?
Hospers-Brands, A.J.T.M. ; Burgt, G.J.H.M. van der - \ 2009
melk - biologische voedingsmiddelen - melksamenstelling - onverzadigde vetzuren - linolzuur - kristallografie - luminescentie - vergelijkend onderzoek - omega-3 vetzuren - milk - organic foods - milk composition - unsaturated fatty acids - linoleic acid - crystallography - luminescence - comparative research - omega-3 fatty acids
Recent studies have indicated possible positive interactions between organic animal production and, particularly, and various vitamins. As possible distinguishing quality parameters for organic milk, the differences between organic and conventional milk in Netherlands for fatty acid composition and vitamins were investigated in milk samples form supermarkets at several points in time. We have also investigated possible differences in taste and two alternative analytical parameters (bio-photons and bio-crystallisations) because a single quality parameter, like poly-unsaturated fatty acids (PUFA), hardly reflects organic intentions to produce quality in a more holistic way being an inherent reflection of proper agricultural practices. These two alternative parameters try to relate to the hypothesis stating that the structure (the ‘order’) of food is just as important to human health as the material composition (Bloksma et al, 2008).
Coaching the process of designing a farm: Using the healthy human as a metaphor for farm health
Bloksma, J.R. ; Struik, P.C. - \ 2007
NJAS Wageningen Journal of Life Sciences 54 (2007)4. - ISSN 1573-5214 - p. 413 - 429.
agrarische bedrijfsvoering - duurzaamheid (sustainability) - gezondheid - identiteit - farm management - sustainability - health - identity
This paper is on coaching farmers in (re)designing a farm. It describes how farmers can become inspired to design a sustainable farm by the way medical doctors look at the human being and his health. Sustainability in farm management is usually strived for in such a way that damage to People, Planet or Profit is prevented. However, preventing such damage does not automatically mean that humans, animals, rural societies, landscape, and nature can develop and manifest themselves to the full extent. Only in examples where the emphasis is on positive characteristics of and actions in farming, and where the approach is based on coherence between the three layers described (agricultural production, agri-culture, and the meaning of life and existence), we observe that all stakeholders can fully express themselves. This approach is comparable with the positive way in which the World Health Organization describes human health, distinguishing three different layers of health: physical health, socio-cultural health, and mental health. Applying the metaphor of the healthy human enables us to ask relevant questions as to how to achieve farm health. The power of the metaphor of the living human lies in the possibility to introduce the concepts of life, the conscious direction, transformation and health in the process of (re)designing the farm organism. The living farm emerges under the direction of the farmer as a new entity in which the possibilities of the site, the societal and agro-ecological context and the people involved are all mutually fine-tuned. Only if all three interconnected layers of health (i.e., the physical, socio-cultural, and mental health) are equally addressed, true farm health, and, in our definition of farm health, also sustainability can be achieved. This paper does not address the issue of creating an absolutely healthy and sustainable farm but is about farm transformation and the art of development, step-by-step reshaping specific farm elements, thus allowing the entire farm to become healthier
The mercapturic acid biotransformation pathway of hexachlorobenzene is not involved in the induction of splenomegaly, skin and lung lesions in the Brown Norway rat
Michielsen, C.P.P.C. ; Mil, F. van; Boeren, S. ; Rietjens, I.M.C.M. ; Bloksma, M.A. ; Vos, J.G. - \ 2000
Archives of Toxicology 74 (2000). - ISSN 0340-5761 - p. 609 - 617.
Involvement of the mercapturic acid pathway in the induction of splenomegaly and skin and lung pathology by hexachlorobenzene (HCB) in the rat was investigated by seeking to determine whether pentachloronitrobenzene (PCNB) has the same inflammatory effects as HCB, since both compounds are directly conjugated to glutathione, and further processed into the same mercapturic acid metabolites which are excreted via the urine. Female Brown Norway (BN/SsNOlaHsd) rats at 3 to 4 weeks of age were orally exposed to diets with or without supplementation with 450 mg HCB or equimolar (467 mg) or higher (934 mg) amounts of PCNB per kilogram of diet over 4 weeks. Gross skin lesion development and body weight gains were assessed during exposure and spleen and liver weights as well as histopathologic changes in skin and lung were assessed after exposure. After 3 weeks of exposure, urinary metabolites of the mercapturic acid and oxidative biotransformation pathways were identified using high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS). Oral exposure of the rats to 450 mg/kg HCB resulted in an increase in relative spleen and liver weights as well as in the development of skin and lung pathology in the absence of overall liver toxicity. Equimolar or higher concentrations of PCNB caused none of these effects. Urinary levels of the mercapturic acid N-acetyl-S-(pentachlorophenyl)-cysteine (PCP-NAC), were comparable in HCB- and PCNB-treated rats. Levels of closely related methylsulfide derivatives of PCP-NAC, also generated via the same mercapturic acid pathway, appeared to be significantly higher in PCNB- than in HCB-treated rats, whereas the reverse was true for the urinary levels of the oxidative metabolite pentachlorophenol (PCP). Thus, results indicate that metabolites of the mercapturic acid pathway are not involved in the induction of splenomegaly and skin and lung pathology caused by HCB exposure in BN rats and that the main urinary metabolite of HCB in these BN rats is PCP. Since PCP itself, as well as other cytochrome P450-derived metabolites from HCB, are not likely to be involved in the induction of splenomegaly and skin and lung pathology, it is suggested that either the parent compound HCB or as-yet-unidentified non-P450-generated metabolites are involved in these inflammatory effects of HCB.
A protoplast system for studying tomato spotted wilt virus infection.
Kikkert, M. ; Poelwijk, F. van; Karssies, W. ; Bloksma, H. ; Storms, M. ; Lent, J. van; Kormelink, R. ; Goldbach, R. - \ 1997
Journal of General Virology 78 (1997). - ISSN 0022-1317 - p. 1755 - 1763.
|A protoplast system for studying tomato spotted wilt virus infection.
Kikkert, M. ; Poelwijk, F. van; Storms, M. ; Bloksma, H. ; Kormelink, R. ; Goldbach, R. - \ 1996
In: Abstract 10th Int. Congr. of Virology. Jerusalem, Israel (1996) 83. Ook: Abstract NWO-SON bijeenkomst, Lunteren - p. 55 - 55.
|Initial interactions between virus and plant cells.
Verduin, B.J.M. ; Bloksma, H.R. ; Gubbels, M.J. ; Roenhorst, J.W. ; Jacobs, G.T. - \ 1995
In: Int. Symp. 75 Years of Phytopathological and Resistance Research at Aschersleben, Germany (1995)
|Expression of the movement protein of two bromoviruses in insect cells using a baculovirus expression system.
Verduin, B.J.M. ; Bloksma, H.R. ; Goldbach, R.W. ; Hamelink, W. ; Kleima, F. ; Usmany, M. ; Wolfs, C.J.A.M. - \ 1994
In: Abstract Am. Soc. Virology. Madison - p. 146 - 146.
Strain hardening of dough as a requirement for gas retention.
Vliet, T. van; Jansen, A.M. ; Bloksma, A.H. ; Walstra, P. - \ 1992
Journal of Texture Studies 23 (1992). - ISSN 0022-4901 - p. 439 - 460.
|Role of bromovirus 3a protein in transport and relation with host range specificity.
Verduin, B.J.M. ; Bloksma, H.R. - \ 1992
Netherlands Journal of Plant Pathology 98 (1992). - ISSN 0028-2944 - p. 7 - 7.
|Exchanges of domains within the 1A protein from two bromoviruses and their effect on viral replication in barley and cowpea.
Verduin, B.J.M. ; Bloksma, H.R. ; Kroner, P. ; Traynor, P.L. ; Ahlquist, P. - \ 1990
In: Abstract 8th Int. Congr. Virology, Berlin - p. 479 - 479.
Verduin, B.J.M. ; Bloksma, H.R. ; Coenen, M.M.G. ; Goldbach, R.W. ; Jacobs, S.G.T. ; Roenhorst, J.W. - \ 1990
In: Abstract 8th Int. Congr. Virology, Berlin - p. 124 - 124.
|Fosfaatopname en beworteling
Werff, P.A. van der; Bloksma, J. - \ 1985
Ekoland 6 (1985)2. - ISSN 0926-9142 - p. 23 - 25.
|Virus multiplication in cowpea leaves after differential temperature inoculation
Verduin, B.J.M. ; Bloksma, H.R. ; Vandebriel, R.J. - \ 1982
Acta botanica neerlandica 31 (1982). - ISSN 0044-5983 - p. 246 - 246.