Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Active video games as a tool to prevent excessive weight gain in adolescents: rationale, design and methods of a randomized controlled trial
    Simons, M. ; Chinapaw, M.J.M. ; Bovenkamp, M. van de; Boer, M.R. de; Seidell, J.C. ; Brug, J. ; Vet, E. de - \ 2014
    BMC Public Health 14 (2014). - ISSN 1471-2458 - 13 p.
    promote physical-activity - sedentary screen time - body-mass index - intrinsic motivation - self-determination - economic burden - obesity - children - overweight - youth
    Background Excessive body weight, low physical activity and excessive sedentary time in youth are major public health concerns. A new generation of video games, the ones that require physical activity to play the games –i.e. active games- may be a promising alternative to traditional non-active games to promote physical activity and reduce sedentary behaviors in youth. The aim of this manuscript is to describe the design of a study evaluating the effects of a family oriented active game intervention, incorporating several motivational elements, on anthropometrics and health behaviors in adolescents. Methods/Design The study is a randomized controlled trial (RCT), with non-active gaming adolescents aged 12 – 16 years old randomly allocated to a ten month intervention (receiving active games, as well as an encouragement to play) or a waiting-list control group (receiving active games after the intervention period). Primary outcomes are adolescents’ measured BMI-SDS (SDS =¿adjusted for mean standard deviation score), waist circumference-SDS, hip circumference and sum of skinfolds. Secondary outcomes are adolescents’ self-reported time spent playing active and non-active games, other sedentary activities and consumption of sugar-sweetened beverages. In addition, a process evaluation is conducted, assessing the sustainability of the active games, enjoyment, perceived competence, perceived barriers for active game play, game context, injuries from active game play, activity replacement and intention to continue playing the active games. Discussion This is the first adequately powered RCT including normal weight adolescents, evaluating a reasonably long period of provision of and exposure to active games. Next, strong elements are the incorporating motivational elements for active game play and a comprehensive process evaluation. This trial will provide evidence regarding the potential contribution of active games in prevention of excessive weight gain in adolescents.
    Beweeggames: kansen voor het stimuleren van beweging?
    Simons, M. ; Bovenkamp, M. van de; Vet, E.W.M.L. de - \ 2014
    In: Van Tikken naar Taggen: digitalisering van bewegingsonderwijs en sport / van Hilvoorde, I., Kleinpaste, J., Deventer : daM Uitgeverij (Sport en Kennis ) - ISBN 9789071902116 - p. 205 - 217.
    First report of potato blackleg caused by Pectobacterium carotovorum subsp. brasiliensis in the Netherlands
    Nunes Leite, L. ; Haan, E.G. de; Krijger, M.C. ; Kastelein, P. ; Zouwen, P.S. van der; Bovenkamp, G.W. van den; Tebaldi, N.D. ; Wolf, J.M. van der - \ 2014
    New Disease Reports 29 (2014)2014. - ISSN 2044-0588 - p. 24 - 24.
    Double-layer crystal violet pectate (DL-CVP) medium (Helias et al., 2012) was used to isolate pectinolytic bacteria from the stems of blackleg affected plants. Eleven isolates derived from ten seed lots, positive in the Pcb-specific PCR (Duarte et al., 2004), were characterised with multilocus sequence analysis (MLSA) using sequences of acnA, gapA, icdA, mdh, mtlD, pgi and proA (Panda et al., 2012) (GenBank Accession Nos. KJ576801-KJ576810, KJ596350-KJ596399, KJ608062-KJ608071). The phylogenetic analysis was done by constructing a maximum likelihood tree using sequences of reference strains P. atrosepticum, P. wasabiae, P.c. subsp. carotovorum, P.c. subsp. odororiferum, P. betavasculorum and Dickeya paradisiaca, derived from GenBank, for comparison (Fig. 1). The MLSA confirmed the identity of the Pcb strains. The Dutch strains clustered together in a homogeneous branch, which also showed that these strains were highly similar. Strains were motile, able to reduce sucrose and to grow at 39°C, resistant to erythromycin, and unable to ferment lactose according to the procedures described by Hayman et al. (1998). Two Pcb isolates were tested for virulence in a field experiment in the Netherlands in 2013. Two months after planting vacuum-infiltrated seed tubers, a high percentage of the plants (more than 30%) showed typical symptoms, whereas water-treated plants remained disease-free (Fig. 2). The pathogen was re-isolated from the diseased plants and identified as Pcb using MLSA. This is the first time Pcb is described as a potato blackleg-causing agent in the Netherlands, although a strain of Pcb isolated from potato in the Netherlands has been found in a culture collection before (De Boer et al., 2012).
    Development and evaluation of Taqman assays for the differentiation of Dickeya (sub)species
    Wolf, J.M. van der; Haas, B.H. de; Hoof, R.A. van; Haan, E.G. de; Bovenkamp, G.W. van den - \ 2014
    European Journal of Plant Pathology 138 (2014)4. - ISSN 0929-1873 - p. 695 - 709.
    complete genome sequence - syn. erwinia-chrysanthemi - potato - strains - pcr - relatedness - polymerase - clade - crops - hosts
    TaqMan assays were developed for the detection of seven Dickeya species, namely D. dianthicola, D. dadantii, D. paradisiaca, D. chrysanthemi, D. zeae, D. dieffenbachiae and D. solani. Sequences of the gene coding for dnaX were used for the design of primers and probes. In studies with axenic cultures of bacteria, the assays were highly specific and only reacted with strains of the target species, and not with non-target bacteria, including those belonging to other Dickeya species and other genera. The detection thresholds for DNA extracted from pure cultures of target strains ranged from 10 to 100 fg. The TaqMan assays for D. dianthicola and D. solani were more extensively evaluated as part of a method validation procedure. The threshold level for target bacteria added to a potato peel extract diluted ten-times in a semi-selective broth, was strain dependent and ranged from 1,000 to 100,000 cfu/ml. The coefficients of variation for repeatability and reproducibility were low and results were largely independent of the type of substrate, i.e. potato tuber or carnation leaf extracts. However, during routine testing of seed potatoes, false-positive reactions were found with the assay for D. solani. The use of the TaqMan assays for inspection of plant propagation material, ecological studies and studies on the effect of control strategies in disease management strategies is discussed
    Characterization of Dutch strains of Pectobacterium carotovorum subsp. brasiliensis, a causative agent of potato blackleg
    Nunes-Leite, L. ; Krijger, M.C. ; Kastelein, P. ; Zouwen, P.S. van der; Haan, E.G. de; Bovenkamp, G.W. van den; Tebaldi, N.D. ; Wolf, J.M. van der - \ 2013
    Deltaplan Erwinia. Deel C - Pootaardappelen. Eindrapport van het onderzoek 2009 - 2012
    Boomsma, D. ; Velvis, H. ; Kristelijn, K. ; Tent Becking, T. van; Kastelein, P. ; Zouwen, P.S. van der; Krijger, M.C. ; Förch, M.G. ; Wolf, J.M. van der; Czajkowski, R.L. ; Jafra, S. ; Bovenkamp, G. van den; Haan, E. de; Nunes Leite, L. - \ 2013
    Den Haag, Zoetermeer : Nederlands Aardappel Organisatie (NAO), Productschap Akkerbouw (PA) - 104
    erwinia - pectobacterium - dickeya - plantenziekteverwekkende bacteriën - plantenziekten - plantenziektebestrijding - aardappelen - akkerbouw - landbouwkundig onderzoek - besmetting - ziektepreventie - gewasbescherming - erwinia - pectobacterium - dickeya - plant pathogenic bacteria - plant diseases - plant disease control - potatoes - arable farming - agricultural research - contamination - disease prevention - plant protection
    Het Deltaplan Erwinia (Deel C – Pootaardappelen) is een vervolg op het project 'Bacterievrije pootgoedteelt - een uitdaging!'. Het doel was om meer inzicht te krijgen in de Erwinia-problemen in de pootgoedteelt en uiteindelijk te komen tot een reductie van de economische schade met 50%. Uit het onderzoek (2009-2012) blijkt dat er bij vrijwel elk onderdeel van de teeltcyclus van pootaardappelen risicovolle handelingen vallen aan te wijzen die tot verergering van het probleem leiden. Na vier jaar onderzoek is de conclusie dat er, om de ten doel gestelde reductie van 50% reductie te realiseren, een samenhangend pakket aan maatregelen nodig is. Daarvoor wordt een aantal aanbevelingen gedaan
    Virulence of ‘Dickeya solani’ and Dickeya dianthicola biovar-1 and -7 strains on potato (Solanum tuberosum)
    Czajkowski, R.L. ; Boer, W.J. de; Zouwen, P.S. van der; Kastelein, P. ; Jafra, S. ; Haan, E.G. de; Bovenkamp, G.W. van den; Wolf, J.M. van der - \ 2013
    Plant Pathology 62 (2013)3. - ISSN 0032-0862 - p. 597 - 610.
    soft-rot erwinias - carotovora subsp atroseptica - plant-pathogenic bacteria - host-range - pectobacterium - israel - extracts
    Studies were conducted to explain the relative success of ‘Dickeya solani’, a genetic clade of Dickeya biovar 3 and a blackleg-causing organism that, after recent introduction, has spread rapidly in seed potato production in Europe to the extent that it is now more frequently detected than D. dianthicola. In vitro experiments showed that both species were motile, had comparable siderophore production and pectinolytic activity, and that there was no antagonism between them when growing. Both ‘D. solani’ and biovar 1 and biovar 7 of D. dianthicola rotted tuber tissue when inoculated at a low density of 103 CFU mL-1. In an agar overlay assay, D. dianthicola was susceptible to 80% of saprophytic bacteria isolated from tuber extracts, whereas ‘D. solani’ was susceptible to only 31%, suggesting that ‘D. solani’ could be a stronger competitor in the potato ecosystem. In greenhouse experiments at high temperatures (28°C), roots were more rapidly colonized by ‘D. solani’ than by biovar 1 or 7 of D. dianthicola and at 30 days after inoculation higher densities of ‘D. solani’ were found in stolons and progeny tubers. In co-inoculated plants, fluorescent protein (GFP or DsRed)-tagged ‘D. solani’ outcompeted D. dianthicola in plants grown from vacuum-infiltrated tubers. In 3 years of field studies in the Netherlands with D. dianthicola and ‘D. solani’, disease incidence varied greatly annually and with strain. In summary, ‘D. solani’ possesses features which allow more efficient plant colonization than D. dianthicola at high temperatures. In temperate climates, however, tuber infections with ‘D. solani’ will not necessarily result in a higher disease incidence than infections with D. dianthicola, but latent seed infection could be more prevalent
    Genotypic Diversity of Coxiella burnetii in the 2007-2010 Q Fever Outbreak Episodes in The Netherlands
    Tilburg, J.J.H.C. ; Rossen, J.W.A. ; Hannen, E.J. van; Melchers, W.J.G. ; Hermans, M.H.A. ; Bovenkamp, J. van de; Roest, H.I.J. ; Bruin, A. de; Nabuurs-Fransen, M.H. ; Horrevorts, A.M. ; Klaassen, C.H.W. - \ 2012
    Journal of Clinical Microbiology 50 (2012)3. - ISSN 0095-1137 - p. 1076 - 1078.
    pcr
    The genotypic diversity of Coxiella burnetii in clinical samples obtained from the Dutch Q fever outbreak episodes of 2007-2010 was determined by using a 6-locus variable-number tandem repeat analysis panel. The results are consistent with the introduction of one founder genotype that is gradually diversifying over time while spreading throughout The Netherlands.
    Goed om uitgangsmateriaal op Erwinia te testen (interview met Jan van der Wolf)
    Dwarswaard, A. ; Bovenkamp, G. van den; Wolf, J.M. van der - \ 2012
    BloembollenVisie (2012)241. - ISSN 1571-5558 - p. 23 - 23.
    erwinia - bacterieziekten - pootaardappelen - bloembollen - landbouwkundig onderzoek - testen - vermeerderingsmateriaal - bacterial diseases - seed potatoes - ornamental bulbs - agricultural research - testing - propagation materials
    Bloembollen en aardappelen. Ze hebben in ieder geval één ziekte gemeen: bacterierot, veroorzaakt door Erwinia. In beide teelten zorgen deze bacterieziekten voor veel schade. In het Deltaplan Erwinia werken de bloembollen- en aardappelwereld samen op onderzoeksgebied. In twee artikelen staat de vraag centraal wat de bollen en de aardappelen van elkaar hebben geleerd. Deel 2: Gé van den Bovenkamp van de NAK en Jan van der Wolf van PRI.
    Determination of aphid transmission efficiencies for N, NTN and Wilga strains of Potato virus Y
    Verbeek, M. ; Piron, P.G.M. ; Dullemans, A.M. ; Cuperus, C. ; Bovenkamp, G. van den; Vlugt, R.A.A. van der - \ 2010
    precisielandbouw - aardappelen - akkerbouw - virusziekten - aardappelvirus y - myzus persicae - aphididae - insectenbestrijding - insectenetende dieren - verspreiders - precision agriculture - potatoes - arable farming - viral diseases - potato virus y - myzus persicae - aphididae - insect control - insectivores - distributors
    REFs (Relative Efficiency Factor) are used in the Dutch PVY (Potato virus Y genus Potyvirus) control system to calculate vector pressure and determine the haulm destruction date.
    Determination of aphid transmission efficiencies for N, NTN and Wilga strains of Potato virus Y
    Verbeek, M. ; Piron, P.G.M. ; Dullemans, A.M. ; Cuperus, C. ; Bovenkamp, G. van den; Vlugt, R.A.A. van der - \ 2010
    In: Book of abstracts EAPR 2010. 14th Triennial meeting of the Virology Section of the European Association for Potato Research (EAPR), Hamar, Norway, 4-9 July 2010. - Ås, Norway : Bioforsk - ISBN 9788217006640 - p. 44 - 44.
    Potato virus Y (PVY, genus Potyvirus, family Potyviridae) causes high economic losses worldwide, especially in the production of seed potatoes (Solanum tuberosum). PVY control systems rely on measuring virus pressure and vector pressure in the field. Calculation of the vector pressure is based on the relative efficiency factors (REFs) of aphid species, which express the transmission efficiency of aphid species in relation to the efficiency of Myzus persicae, the most efficient vector of PVY. In the Netherlands, in the 1980s, aphid’s REFs were determined using aphids caught alive in the field. Thus, experiments were conducted using limited numbers of aphids and only during the potato growing season. We have now developed a system which allows us to test virus transmission whole year round, using aphid clones reared in insect chambers
    Mucin-bacterial interactions in the human oral cavity and digestive tract
    Derrien, M.M.N. ; Passel, M.W.J. van; Bovenkamp, J.H.B. van de; Schipper, R.G. ; Vos, W.M. de - \ 2010
    Gut Microbes 1 (2010)4. - ISSN 1949-0976 - p. 254 - 268.
    Mucins are a family of heavily glycosylated proteins that are the major organic components of the mucus layer, the protective layer covering the epithelial cells in many human and animal organs, including the entire gastro-intestinal tract. Microbes that can associate with mucins benefit from this interaction since they can get available nutrients, experience physico-chemical protection and adhere, resulting an increased residence time. Mucin-degrading microorganisms, which often are found in consortia, have not been extensively characterized as mucins are high molecular weight glycoproteins that are hard to study because of their size, complexity, and heterogeneity. The purpose of this review is to discuss how advances in mucus and mucin research, and insight in the microbial ecology promoted our understanding of mucin degradation. Recent insight is presented in mucin structure and organization, the micro-organisms known to use mucin as growth substrate, with a specific attention on Akkermansia muciniphila, and the molecular basis of microbial mucin degradation owing to availability of genome sequences
    Toetsing op Erwinia in hyacint : nu mogelijk bij de NAK
    Doorn, J. van; Vreeburg, P.J.M. ; Martin, W.S. ; Miglino, R. ; Bovenkamp, G. van den; Haan, E. de - \ 2010
    BloembollenVisie 2010 (2010)192. - ISSN 1571-5558 - p. 26 - 27.
    bloembollen - bollen - gewasbescherming - dickeya chrysanthemi - plantenziekteverwekkende bacteriën - testen - bemonsteren - technieken - landbouwkundig onderzoek - ornamental bulbs - bulbs - plant protection - dickeya chrysanthemi - plant pathogenic bacteria - testing - sampling - techniques - agricultural research
    Naast het uitvoeren van een thuistoets op Erwinia kan de teler nu ook zijn partijen laten testen bij de NAK in Emmeloord met een PCR-toets. In dit artikel wordt uitgelegd hoe de toets werkt en hoe nauwkeurig hij is.
    Toetsen op agressief snot (Dickeya) bij de NAK?
    Doorn, J. van; Vreeburg, P.J.M. ; Martin, W.S. ; Korsuize, C.A. ; Schadewijk, T. van; Miglino, R. ; Bovenkamp, G. van den; Haan, E. de - \ 2010
    dickeya chrysanthemi - hyacinten - testen - bloembollen - dickeya chrysanthemi - hyacinths - testing - ornamental bulbs
    Poster met onderzoeksinformatie over de ontwikkeling van een toets om Dickeya in hyacintenbollen vast te stellen .
    Aphid vectors and transmission of Potato virus Y strains
    Verbeek, M. ; Piron, P.G.M. ; Dullemans, A.M. ; Bovenkamp, G.W. van den; Miedema, G. ; Vlugt, R.A.A. van der - \ 2009
    In: Fifth Joint meeting of the DPG working group “Viruskrankheiten der Pflanzen” and the “ Nederlandse Kring voor Plantevirologie”, Hamburg, Germany, 8-9 April 2009. - Hamburg, Germany : Deutsche Phytomedizinische Gesellschaft - p. P18 - P18.
    In the last 5 years Dutch seed potato growers have faced increasing problems with Potato virus Y (PVY) despite a decrease over the past 20 years in the number of aphids caught during the potato-growing season. In the Netherlands the control system for PVY is based on virus monitoring in the field, flight data of aphids and postharvest control. Aphids are caught, counted and assigned a value according to their relative efficiency factor (REF). The aphid flight data are used to set dates for haulm destruction. Given the current problems with PVY, this system, developed in the 1980s, may no longer be adequate to control the current virus situation in the field. Although it was found that REFs of different aphids are still valid for transmission of PVYN, higher transmission rates were found for PVYNTN and PVYN-Wilga. This could explain the increasing PVY infection in the field
    Non-persistente virusoverdracht door bladluizen, Aardappelvirus Y in aardappel
    Verbeek, M. ; Piron, P.G.M. ; Cuperus, C. ; Dullemans, A.M. ; Wiegers, G.L. ; Bruin, A. de; Raaij, H.M.G. van; Vlugt, R.A.A. van der; Bovenkamp, G. van den; Haan, E. de; Miedema, G. ; Stolte, T. - \ 2009
    Wageningen : Plant Research International - 23
    aardappelvirus y - stammen (biologisch) - aphididae - aardappelen - epidemiologie - ziekteoverdracht - akkerbouw - potato virus y - strains - aphididae - potatoes - epidemiology - disease transmission - arable farming
    De laatste jaren nemen de problemen met Aardappelvirus Y (PVY) toe. Dit is terug te vinden in de toenemende percentages declassering van partijen pootaardappelen. Deze tendens is niet te verklaren met behulp van de vangstcijfers van bladluizen, de overbrengers van het virus. De bladluisvangsten nemen de laatste jaren juist af. Om de oorzaak van de toenemende problemen met PVY te achterhalen, startte in 2006 het proejct "Non-persistente overdracht door bladluizen, aardappel", gezamenlijk gefinancierd door het Ministerie van LNV, de NAK en het productschap Akkerbouw (PA). Het onderzoek richtte zich op een drietal vragen: •Is de bladluissituatie in het veld veranderd? Met andere woorden, zijn er nieuwe bladluissoorten aanwezig die PVY kunnen overdragen? •Zijn er andere of nieuwe stammen van het virus in het veld aanwezig? •Is de overdrachtsefficiëntie van bladluizen voor nieuwe PVY-stammen anders? Er is aangetoond dat in de afgelopen jaren de veldsituatie voor wat betreft aanwezige PVY-stammen is veranderd. Enkele jaren geleden werd nog algemeen aangenomen dat voornamelijk PVYN in de Nederlandse aardappelteelt voorkwam. Nu komt deze stam nauwelijks meer voor en zijn PVYO en de recombinante stammen PVYNTN en PVYN-Wi volop aanwezig. Gedurende drie jaar zijn de bladluisvangsten door de NAK uitgebreid geanalyseerd op het eventueel voorkomen van nieuwe soorten. De cijfers zijn vergeleken met de vangstcijfers uit de tachtiger jaren van de vorige eeuw. Nieuwe soorten bladluizen die PVY kunnen overbrengen zijn niet waargenomen. Er is één nieuwe soort gevonden (Utamphorophora humboldti), maar van deze soort is nog niet bekend of het PVY kan overbrengen en, zo ja, met welke efficiëntie. Dit kon helaas niet in dit project worden uitgezocht. Er is een nieuwe methode ontwikkeld om de relatieve transmissie efficiëntie (REF-waarde) van bladluissoorten te meten. Deze methode heeft als voordeel dat hij het gehele jaar door kan worden uitgevoerd. Daarnaast is de methode minder arbeidsintensief in vergelijking met de in de tachtiger jaren gebruikte methode. Van de belangrijkste bladluissoorten is de REF-waarde bepaald voor de overdracht van PVYN, PVYNTN en PVYN-Wi. De waarden van overdracht van PVYN komen goed overeen met de in de tachtiger jaren in Nederland bepaalde REF-waarden, die toen ook met PVYN zijn bepaald. Bij sommige bladluissoorten worden andere REF-waarden gevonden wanneer PVYNTN en PVYN-Wi worden overgedragen. Voor deze soorten zal voorgesteld worden nieuwe REF-waarden in gebruik te nemen voor bepaling van de advies-loofdodingsdatum
    Overdracht Aardappelvirus Y door bladluizen in aardappel
    Verbeek, M. ; Piron, P.G.M. ; Cuperus, C. ; Dullemans, A.M. ; Wiegers, G.L. ; Bruin, A. de; Raaij, H.M.G. van; Vlugt, R.A.A. van der; Bovenkamp, G. van den; Haan, E. de; Miedema, G. ; Stolte, T. - \ 2009
    Kennisakker.nl 2009 (2009)20 mei.
    aardappelen - pootaardappelen - aphididae - aardappelvirus y - onderzoek - akkerbouw - potatoes - seed potatoes - aphididae - potato virus y - research - arable farming
    De laatste jaren nemen de problemen met Aardappelvirus Y (PVY) toe. Om de oorzaak hiervan te achterhalen, is in 2006 onderzoek gestart. Dit onderzoek heeft het volgende geleerd: De veldsituatie voor wat betreft aanwezige PVY-stammen is veranderd; Nieuwe soorten bladluizen die PVY kunnen overdragen zijn niet gevonden; Bij sommige bladluissoorten worden andere REF-waarden gevonden wanneer PVYNTN en PVYN-Wi worden overgedragen. Voor deze soorten zal de NAK andere REF-waarden gaan gebruiken voor de vaststelling van de loofdodingsadviesdatum.
    Nederlands onderzoek aan plantpathogene bacteriën in perspectief : themanummer fytobacterologie
    Wolf, J.M. van der; Bovenkamp, G. van den - \ 2009
    Gewasbescherming 40 (2009)4. - ISSN 0166-6495 - p. 162 - 168.
    plantenziekten - plantenziekteverwekkende bacteriën - vermeerderingsmateriaal - plantenziektebestrijding - elisa - gewasbescherming - aardappelen - bloembollen - koolsoorten - tomaten - fragaria - boomteelt - fruitteelt - wetenschappelijk onderzoek - diagnostiek - plant diseases - plant pathogenic bacteria - propagation materials - plant disease control - elisa - plant protection - potatoes - ornamental bulbs - cabbages - tomatoes - fragaria - arboriculture - fruit growing - scientific research - diagnostics
    Ziekten veroorzaakt door plantpathogene bacteriën worden door producenten van uitgangsmateriaal, door telers en door exporteurs gezien als zeer bedreigend. De ziekteverwekker is vaak moeilijk op te sporen, kan zich symptoomloos verspreiden,is in plantmateriaal niet of moeilijk te bestrijden, resistentiebronnen zijn veelal onbekend en de economische schade bij uitbraak is vaak aanzienlijk. Onderzoek aan plantpathogene bacteriën in Nederland kent dan ook een lange historie. In deze schets wordt het belang van dit onderzoek voor het voetlicht gebracht. Er wordt stilgestaan bij het onderzoek uit heden en verleden. Ook worden kennisleemtes en onderzoekswensen geïnventariseerd
    Aardappelvirus Y en bladluizen: samen efficiënt
    Verbeek, M. ; Vlugt, R.A.A. van der; Piron, P.G.M. ; Cuperus, C. ; Dullemans, A.M. ; Bovenkamp, G.W. van den - \ 2009
    pootaardappelen - plantenvirussen - aardappelvirus y - aardappelen - aphididae - ziekteoverdracht - seed potatoes - plant viruses - potato virus y - potatoes - aphididae - disease transmission
    Onderzoek en resultaten naar de factoren die verantwoordelijk kunnen zijn voor de sterke toename van PVY in de pootaardappelteelt
    Aardappelvirus Y: geen oud probleem
    Verbeek, M. ; Vlugt, R.A.A. van der; Cuperus, C. ; Piron, P.G.M. ; Dullemans, A.M. ; Bovenkamp, G. van den - \ 2008
    In: 5e Gewasbeschermingsmanifestatie, de Reehorst, Ede, Nederland, 22 mei 2008. - Wageningen : KNPV - p. 24 - 24.
    aardappelvirus y - plantenvirussen - pootaardappelen - akkerbouw - geïntegreerde plagenbestrijding - epidemiologie - potato virus y - plant viruses - seed potatoes - arable farming - integrated pest management - epidemiology
    De laatste jaren nemen de problemen met aardappelvirus Y (PVY) toe. Dit is terug te vinden in de toenemende percentages declassering van partijen pootaardappelen. Deze tendens is niet te verklaren met behulp van de vangstcijfers van bladluizen, de overbrengers van het virus. De bladluisvangsten nemen de laatste jaren juist af
    Strains of Potato virus Y in Dutch seed potato culture
    Vlugt, R.A.A. van der; Verbeek, M. ; Piron, P.G.M. ; Cuperus, C. ; Bovenkamp, G. van den; Haan, E. de - \ 2008
    In: Book of Abstracts. The 3rd Conference of the International Working Group on Legume and Vegetable viruses (IWGLVV), Ljubljana, Slovenia, 20-23 August 2008. - Ljubljana, Slovenia : National Institute of Biology (NIB), Department of Biotechnology and Systems Biology - p. 31 - 31.
    Over the recent years Potato virus Y presents a growing problem in Dutch seed potato culture. In recent years a significant % of seed potato lots was de-classified due to PVY infections. This apparent increase in PVY infections was unexpected since no increase in field symptoms were observed and the numbers of aphids caught in the yellow water traps and high suction traps showed a clear decline over the last 10 years.. In 2006 a project was initiated to investigate the possible causes for the increase in PVY infections. Firstly a survey was performed on potato plant material grown in the control fields of the General Inspection Service (NAK), to determine the distribution of PVY strains, including the ‘new’ PVYNTN and PVYN-Wilga strains. This plant material is representative of Dutch seed potato stocks. In the control fields tubers, which were taken from stocks tested by ELISA, are grown to evaluate the earlier laboratory tests. Samples were tested by ELISA using polyclonal and monoclonal antibodies. All ELISA positive samples were further tested for the presence of PVYN, PVYO, PVYNTN and PVYN-Wilga by two molecular methods and in addition inoculated onto a set of indicator plants. Results showed that PVY strains populations in the Netherlands shifted significantly and that PVYNTN and PVYN-Wilga are more spread then generally assumed. In addition to field monitoring and post-harvest control, the control system for potato viruses, including PVY in the Netherlands, is also based on monitoring the flight data of a selected group of aphids. The aphids caught are counted and assigned a value according to their Relative Efficiency Factor (REF). When the cumulative values reach a certain threshold a date for haulm destruction is set, to prevent virus infections of the tubers. Given the current problems with PVY, this system, developed in the 1980’s, may no longer be sufficient to control the current virus situation in the field. In our project the question is addressed whether a shift in aphid populations has occurred and if the REF’s determined in the 1980’s are still valid for M. persicae and other aphids for the PVY strains found in the Netherlands nowadays. A new system was set up to determine the REF’s for known PVY transmitters and other aphids that are presently caught in the field. Figures of transmission efficiencies of different aphid species for different PVY strains will be presented.
    Strains of Potato virus Y in Dutch seed potato culture
    Vlugt, R.A.A. van der; Verbeek, M. ; Cuperus, C. ; Piron, P.G.M. ; Haan, E. de; Bovenkamp, G.W. van den - \ 2008
    In: Proceedings of the 13th European Association for Potato Research Virology Section Meeting, Coylumbridge (Aviemore), Scotland, United Kingdom, 17-22 June 2007. - - p. 191 - 192.
    Potato virus Y (PVY) is a growing problem in Dutch seed potato culture, with an increasing number of seed potato lots failing to meet quality standards. The reasons for this are unknown. In addition to the well-known PVYN and PVYO strains, several new strains of PVY have been reported, of which PVYNTN and PVYN-Wilga (both characterised as being between PVYN and PVYO) are the best known. In 2006, a survey for PVY strains was done on potato plant material grown in the control fields of the General Inspection Service NAK. This material is representative of Dutch seed potato stocks. In the survey, over 120 samples showing distinct PVY symptoms were collected. Each sample was tested by ELISA using polyclonal antibodies for PVY and monoclonal antibodies to distinguish PVYN from PVYO/PVYC. All ELISA-positive samples were further tested for the presence of PVYN, PVYO, PVYNTN and PVYN-Wilga by two molecular methods and in addition inoculated to a set of indicator plants. The results show that a significant shift in PVY strain populations in the Netherlands has occurred and that PVYNTN and PVYN-Wilga are more widespread than previously assumed
    Aphid vectors and transmission of Potato virus Y strains
    Verbeek, M. ; Piron, P.G.M. ; Dullemans, A.M. ; Bovenkamp, G.W. van den; Miedema, G. ; Vlugt, R.A.A. van der - \ 2008
    In: Proceedings of the 13th European Association for Potato Research Virology Section Meeting, Coylumbridge (Aviemore), Scotland, United Kingdom, 17-22 June 2007. - - p. 192 - 192.
    In the last 5 years Dutch seed potato growers have faced increasing problems with Potato virus Y (PVY) despite a decrease over the past 20 years in the number of aphids caught during the potato-growing season. In the Netherlands the control system for PVY is based on virus monitoring in the field, flight data of aphids and postharvest control. Aphids are caught, counted and assigned a value according to their relative efficiency factor (REF). The aphid flight data are used to set dates for haulm destruction. Given the current problems with PVY, this system, developed in the 1980s, may no longer be adequate to control the current virus situation in the field. Although it was found that REFs of different aphids are still valid for transmission of PVYN, higher transmission rates were found for PVYNTN and PVYN-Wilga. This could explain the increasing PVY infection in the field
    Pectobacterium carotovorum subsp carotovorum can cause potato blackleg in temperate climates
    Haan, E.G. de; Dekker-Nooren, T.C.E.M. ; Bovenkamp, G.W. van den; Speksnijder, A.G.C.L. ; Zouwen, P.S. van der; Wolf, J.M. van der - \ 2008
    European Journal of Plant Pathology 122 (2008)4. - ISSN 0929-1873 - p. 561 - 569.
    soft-rot erwinias - ssp-atroseptica - sp-nov. - epidemiology - disease - tubers - chrysanthemi - strains - tissue - plant
    It is well established that the pectinolytic bacteria Pectobacterium atrosepticum (Pca) and Dickeya spp. are causal organisms of blackleg in potato. In temperate climates, the role of Pectobacterium carotovorum subsp. carotovorum (Pcc) in potato blackleg, however, is unclear. In different western and central European countries plants are frequently found with blackleg from which only Pcc can be isolated, but not Pca or Dickeya spp. Nevertheless, tubers vacuum-infiltrated with Pcc strains have so far never yielded blackleg-diseased plants in field experiments in temperate climates. In this study, it is shown that potato tubers, vacuum-infiltrated with a subgroup of Pcc strains isolated in Europe, and planted in two different soil types, can result in up to 50% blackleg diseased plants.
    Aphid vectors and transmission of potato virus Y strains
    Verbeek, M. ; Piron, P.G.M. ; Dullemans, A.M. ; Vlugt, R.A.A. van der; Bovenkamp, G. van den; Miedema, G. - \ 2008
    aphididae - gewasbescherming - geïntegreerde plagenbestrijding - monitoring - aardappelvirus y - vangmethoden - aphididae - plant protection - integrated pest management - monitoring - potato virus y - trapping
    Aphid vectors and transmission of potato virus Y strains
    Verbeek, M. ; Piron, P.G.M. ; Dullemans, A.M. ; Vlugt, R.A.A. van der; Bovenkamp, G. van den; Miedema, G. - \ 2008
    In the last 5 years Dutch seed potato growers have faced increasing problems with Potato virus Y (PVY) despite a decrease over the past 20 years in the number of aphids caught during the potato-growing season. In the Netherlands the control system for PVY is based on virus monitoring in the field, flight data of aphids and postharvest control. Aphids are caught, counted and assigned a value according to their relative efficiency factor (REF). The aphid flight data are used to set dates for haulm destruction. Given the current problems with PVY, this system, developed in the 1980s, may no longer be adequate to control the current virus situation in the field. Although it was found that REFs of different aphids are still valid for transmission of PVYN, higher transmission rates were found for PVYNTN and PVYN-Wilga. This could explain the increasing PVY infection in the field http://www.njf.nu/site/seminarRedirect.asp?p=1004&intSeminarID=402&strSemInfoType=pro
    Multiplex microsphere immuno-detection of potato virus Y, X and PLRV
    Bergervoet, J.H.W. ; Peters, J. ; Beckhoven, J.R.C.M. van; Bovenkamp, G.W. van den; Jacobson, J.W. ; Wolf, J.M. van der - \ 2008
    Journal of Virological Methods 149 (2008)1. - ISSN 0166-0934 - p. 63 - 68.
    microarray technology - assays
    To monitor seed potatoes for potato virus X, Y and PLRV, a multiplex microsphere immunoassay (MIA) was developed based on the Luminex xMAP technology, as an alternative to ELISA. The xMAP technology allowed detection of a number of antigens simultaneously whereas ELISA only allowed simplex detection of antigens. The use of paramagnetic beads in the MIA procedure allowed efficient removal of excess sample compounds and reagents. This resulted in lower background values and a higher specificity than a non-wash MIA procedure using conventional beads. In a simplex MIA detection, levels for PVY and PLRV in potato leaf extracts were 10 times lower than ELISA but for PVX 10 timers higher, whereas the specificity was similar. Results of a multiplex assay performed on viruses added to potato leaf extracts were largely similar to those of ELISA for individual viruses. Results of samples infected naturally with PVX, PVY or PLRV were comparable with ELISA.
    Strains of Potato virus Y in Dutch seed potato culture
    Vlugt, R.A.A. van der; Verbeek, M. ; Piron, P.G.M. ; Cuperus, C. ; Bovenkamp, G. van den; Haan, E. de - \ 2007
    In: Program and Abstracts. 10th Int Plant Virus Epidemiology Symposium: "Controlling Epidemics of Emerging and Established Plant Virus Diseases - The Way Forward", Icrisat, India, 15-19 October 2007. - Australia and India : International Plant Virus Epidemiology Committee (IPVEC) of International Society of Plant - p. 53 (OP - 52).
    Over the recent years Potato virus Y (PVY) presents a growing problem in Dutch seed potato culture. In the years 2002 – 2004 approximately 10 – 15% of the seed potato lots was declassified because of the levels of PVY infection exceeded quality standards. In 2006 an even higher percentage was reported. Aphid vectors form an important factor in the spread of PVY. However, the increasing levels of infections with PVY are in contrast to the decreasing numbers of aphids caught in the yellow water traps and high suction traps used to monitor aphid flights. In addition to the well-known PVY-N and PVY-O standard strains, several new strains of PVY have been reported of which PVY-NTN and PVY-N-Wilga are currently the best known. Both strains are described as genetic recombinants between PVY-N and PVY-O. The presence and possible spread over The Netherlands of these recombinant strains is not known yet. In July 2006 a survey for PVY strains was done on potato plant material grown in the control fields of the General Inspection Service NAK. This plant material is representative of Dutch seed potato stocks. In the control fields tubers, which were taken from stocks tested by ELISA in 2005, are grown to evaluate the earlier laboratory tests by visual inspection. In the survey over 120 samples showing distinct PVY symptoms were collected from different seed lots. Each sample was tested by ELISA using polyclonal antibodies for the presence of PVY and monoclonal antibodies to distinguish PVY-N from PVY-O/C. All ELISA positive samples were further tested for the presence of PVY-N, PVY-O, PVY-NTN and PVY-N-Wilga by two molecular methods and in addition inoculated on a set of indicator plants. Results show that a significant shift in PVY strains populations in the Netherlands has occurred and that PVY-NTN and PVY-N-Wilga are more spread then previously and generally assumed.
    Aphid vectors and transmission of Potato virus Y strains
    Verbeek, M. ; Piron, P.G.M. ; Dullemans, A.M. ; Bovenkamp, G. van den; Vlugt, R.A.A. van der - \ 2007
    In: Controlling Epidemics of Emerging and Established Plant Virus Diseases - The Way Forward. 10th International Plant Virus Epidemiology Symposium, 15-19 October 2007, ICRISAT Patancheru, Hyderabad, India. - - p. 178 - 178.
    Aardappelvirus Y: diversiteit troef
    Verbeek, M. ; Vlugt, R.A.A. van der; Piron, P.G.M. ; Dullemans, A.M. ; Cuperus, C. ; Bovenkamp, G.W. van den - \ 2007
    aardappelen - aardappelvirus y - fytosanitair beleid - potatoes - potato virus y - phytosanitary policies
    Aardappelvirus Y: opnieuw onder de loep
    Vlugt, R.A.A. van der; Verbeek, M. ; Cuperus, C. ; Piron, P.G.M. ; Bovenkamp, G. van den; Haan, E. de; Stolte, T. - \ 2006
    plantenvirussen - aardappelvirus y - solanum tuberosum - aardappelen - pootaardappelen - plant viruses - potato virus y - solanum tuberosum - potatoes - seed potatoes
    Aardappelvirus Y (PVY) is al vele jaren een belangrijk virus in de Nederlandse pootaardappelteelt. Om nog onbekende redenen nemen de besmettingen met dit virus en de daaruit voortvloeiende schade sinds enige jaren aanzienlijk toe. Nagegaan is in dit onderzoek welke factoren verantwoordelijk zijn voor de sterke toename van PVY in het veld
    Variation in retinol and carotenoid content of milk and milk products in the Netherlands
    Hulshof, P.J.M. ; Roekel-Jansen, G.C. van; Bovenkamp, P. van de; West, C.E. - \ 2006
    Journal of Food Composition and Analysis 19 (2006)1. - ISSN 0889-1575 - p. 67 - 75.
    mature human-milk - major carotenoids - vitamin-a - foods - quantitation - infants
    Retinol and carotenoids were measured in Dutch milk and dairy products using a validated approach based on complete extraction of fat, followed by mild saponification and analysis by high-performance liquid chromatography. Raw milk, full fat milk, semi-skimmed milk and butter contain about 10 ¿g retinol and 6 g carotenoids per g fat. Values for retinol equivalents in milk are 10-20% higher than the values published in the Dutch food composition table. ß-Carotene comprises 90% of total carotenoids present in cow's milk, contrary to values published for human milk, which show more equally distributed carotenoids. Winter milk contains 20% less retinol and ß
    Separation and direct detection of long chain fatty acids and their methylesters by the non-aqueous reversed phase HPLC and Silver Ion Chromotography, combined with CO laser pumped thermal lens spectrometry
    Bicanic, D.D. ; Mocnik, G. ; Franko, M. ; Niederlander, H.A.G. ; Bovenkamp, P. van de; Cozijnsen, J.L. ; Klift, E.J.C. van der - \ 2006
    Instrumentation Science and Technology 34 (2006)1-2. - ISSN 1073-9149 - p. 129 - 150.
    performance liquid-chromatography - beta-carotene - lipids - triacylglycerols - deflection - oils
    The potential of the CO laser pumped dual beam thermal lens spectrometer (TLS) used as the detector of infrared (IR) absorbance in non-aqueous reversed-phase high pressure liquid chromatography (NARP-HPLC) and argentation chromatography (Ag-HPLC-TLS) has been investigated. The linoleic acid C18:2 (9,11) cis, cis, the conjugated linoleic acid C18:2 (9,12) cis, cis, and (9,11) cis, cis and oleic acid C18:1 (9) cis free fatty acids present in the test mixture were separated and detected by NARP-HPLC-TLS directly, i.e., without a need for their prederivatization. The limit of detection (LOD) for the present NARP-HPLC-TLS set-up was determined using either oleic acid C18:1 (9) cis or linoleic acid C18:2 (9,12) cis, cis as the only analyte. The LOD obtained at 1750 cm-1 for C18:2 (9,12) cis, cis is 0.14% (v/v) with the standard deviation being 3.8% of the measured value. This is comparable to the LOD attainable by the bulk property refractive index detector (RID). For C18:1 (9) cis the LOD is 0.04% (v/v) with a standard deviation of 2.5%. The unique feature of the NARP-HPLC-TLS is its excellent selectivity; this was demonstrated experimentally by adding alcohols (octanol and decanol) that lack the C&dbnd;O carbonyl group to the aforementioned mixture of three fatty acids. In another experiment, the Ag-HPLC-TLS in IR was used to directly detect two different mixtures of five fatty acid methyl esters (FAMEs) after separation on the column. The results were compared to those achieved by the conventional UV absorbance detector. Despite more favourable excitation cross sections of FAMEs in the IR, the concentration LOD obtained for Ag-HPLC-TLS is inferior to that of the Ag-HPLC-UV. However, the mass LOD (11 µg) achieved by Ag-HPLC-TLS in IR for C18:3 FAME is two times better than that of the Ag-HPLC-UV absorbance detector. High IR absorption of eluent restricts, at present, the sensitivity of the Ag-HPLC-TLS and NARP-HPLC-TLS. Improved LOD's are anticipated by a judicious choice of the mobile phase and after materialising several modifications of the experimental set-up.
    Specific detection of Ralstonia solanacearum 16S rRNA sequences by AmpliDet RNA
    Wolf, J.M. van der; Beckhoven, J.R.C.M. van; Haan, E.G. de; Bovenkamp, G.W. van den; Leone, G.O.M. - \ 2004
    European Journal of Plant Pathology 110 (2004)1. - ISSN 0929-1873 - p. 25 - 33.
    polymerase chain-reaction - pseudomonas-solanacearum - real-time - molecular beacons - pcr - amplification - hybridization - oligonucleotides - multiplex - product
    The potential of AmpliDet RNA for specific detection of Ralstonia solanacearum in potato tuber samples and surface water was demonstrated. AmpliDet RNA is a procedure based on nucleic acid sequence based amplification (NASBA) of RNA sequences and homogeneous real time detection of NASBA amplicons with a molecular beacon. The procedure is carried out in sealed tubes, thus reducing the risks for carry-over contamination. AmpliDet RNA enabled reliable detection of specific 16S rRNA sequences of R. solanacearum in total RNA extracts from potato tuber samples in 90 min at a level of 10 cells per reaction, equivalent to ca. 104 cells ml-1 of sample. In surface water, AmpliDet RNA allowed detection of R. solanacearum at a level of 10 cfu ml-1, after concentrating bacteria from 200 ml of surface water into 1 ml of surface water by centrifugation. All strains of R. solanacearum and a strain of R. syzygii were positive in AmpliDet RNA, but not other (related) bacterial species. Ralstonia solanacearum (race 3, biovar 2) could be detected reliably in 18 naturally infected potato tuber samples containing varying concentrations of cells. Ninety-one negative tuber samples, from which no R. solanacearum was isolated, were tested in AmpliDet RNA, including 23 samples containing bacteria (cross-) reacting with antibodies against R. solanacearum in immunofluorescence (IF) cell-staining. Only one negative sample, containing high numbers of IF-positive cells, was positive in AmpliDet RNA
    Extraction of carotenoids from feces enabling the bioavailability of ß-carotene to be studied in Indonesian children
    Lieshout, M. van; West, C.E. ; Bovenkamp, P. van de; Wang, Y. ; Sun, Y. ; Breemen, R.B. van; Permaesih, D. ; Muhilal, ; Verhoeven, M.A. ; Creemers, A.F.L. ; Lugtenburg, J. - \ 2003
    Journal of Agricultural and Food Chemistry 51 (2003)17. - ISSN 0021-8561 - p. 5123 - 5130.
    mass-spectrometry - retinol - humans - bioefficacy
    Previously, we have presented a method for quantifying beta-carotene bioavailability based on analysis in serum, following administration of C-13-labeled beta-carotene. Because stool samples can be collected noninvasively, we have now extended the method to measure the bioavailability based on measurements in feces. An extraction method was developed to enable measurement of concentrations and degree of isotopic enrichment of retinol, retinyl palmitate and carotenoids in feces. Relative bioavailability of beta-carotene from pumpkin (n = 6) was found to be 1.8 times (interval, 0.6, 5.5) greater than that from spinach (n = 8), based on data from feces compared with 1.7 times (interval, 0.9, 3.1) based on data from serum.
    N-3 fatty acids in human fat tissue aspirates are stable for up to 6y
    Katan, M.B. ; Harryvan, J.L. ; Bovenkamp, P. van de - \ 2003
    European Journal of Clinical Nutrition 57 (2003). - ISSN 0954-3007 - p. 816 - 818.
    subcutaneous adipose-tissue - storage
    Objectives: The content of n-3 (omega-3) polyunsaturated fatty acids in fat tissue is a valid indicator of their long-term consumption. We studied the stability of n-3 fatty acids in human subcutaneous fat microbiopsies after 6 and 11 y of storage. Design: Microbiopsies were taken from a lump of human adipose tissue and stored at + 20 and -80degreesC. Setting: Laboratory study. Results: After 5.6 y at -80degreesC the proportion of six out of seven highly polyunsaturated fatty acids varied between 91 and 102% (mean 97%) of their baseline values. Storage at + 20degreesC yielded recoveries between 82 and 105%. After 11 y at -80degreesC the proportions in the original lump of tissue ranged from 88 to 101% (mean 94%). Conclusion: n-3 fatty acids in stored fat tissue aspirates are stable for 6 - 11 y, and are suitable markers of baseline diet in long-term epidemiological studies. Sponsorship: Wageningen Centre for Food Sciences.
    Eindelijk betrouwbare 'zwartbeentoets'
    Beckhoven, J.R.C.M. van; Wolf, J.M. van der; Hanse, L. ; Bovenkamp, G. van den; Haan, E. de - \ 2002
    Aardappelwereld 56 (2002)7. - ISSN 0169-653X - p. 17 - 19.
    aardappelen - plantenziekteverwekkende bacteriën - erwinia - dickeya chrysanthemi - plantenziekten - elisa - analytische methoden - potatoes - plant pathogenic bacteria - erwinia - dickeya chrysanthemi - plant diseases - elisa - analytical methods
    Met Elisa-toets en een toepasbaar antiserum is het mogelijk de bacterieziekten Erwinia atroseptica en Erwinia chrysanthemi aan te tonen in een partij aardappelen
    Voedingsmiddelenanalyses van de Vakgroep Humane Voeding en Epidemiologie. Deel 12 Retinol en carotenoiden in zuivelproducten
    Hulshof, P.J.H. ; Roekel, T. van; Bovenkamp, P. van de; West, C.E. ; Katan, M.B. - \ 2001
    Unknown Publisher
    voedingsmiddelen - chemische samenstelling - vetten - oliën - bakoliën - bakvetten - boter - margarine - voedingswaarde - voedsel - voedselsamenstelling - kwaliteit - eigenschappen - huishoudens - ziekenhuizen - ziekenhuiscatering - verpleeghuizen - dierlijke producten - ingrediënten - ghana - filippijnen - spijsoliën - spijsvetten - institutionele huishoudens - foods - chemical composition - fats - oils - cooking oils - cooking fats - butter - margarine - nutritive value - food - food composition - quality - properties - households - hospitals - hospital catering - nursing homes - animal products - ingredients - ghana - philippines - edible oils - edible fats - institutional households
    Comparison of bioavailability of lutein and beta-carotene in spinach and pumpkin consumed by Indonesian children
    Lieshout, M. van; West, C.E. ; Bovenkamp, P. van de; Roekel, T. van; Versloot, P. ; Permaesih, D. ; Muhilal, ; Creemers, A.F.L. ; Verhoeven, M.A. ; Lugtenburg, J. ; Wang, Y. ; Sun, Y. ; Xu, X. ; Breemen, R.B. - \ 2001
    In: Report of the XX International Vitamin A Consultative Group meeting
    Voedingsmiddelenanalyses van de afdeling Humane Voeding en Epidemiologie : deel 13 Retinol en carotenoïden in zuivelproducten
    Hulshof, P.J.M. ; Roekel, T. van; Bovenkamp, P. van de; West, C.E. - \ 2001
    Wageningen : Wageningen UR, Afdeling Humane Voeding & Epidemiologie - ISBN 9789067546584 - 78 p.
    Detection of Ralstonia solanacearum biovar 2 in potato by serological methods based on recombinant monoclonal antibodies and AmpliDet RNA
    Wolf, J.M. van der; Leone, G.O.M. ; Beckhoven, J.C.R.M. van; Bentsink, L. ; Haan, E.G. de; Bovenkamp, G.W. van den; Griep, R.A. ; Twisk, C. van; Schots, A. - \ 1999
    In: Beiträge zur Zürchtungsforschung, Proceedings of the International Symposium on New Aspects of Resistance Research on Cultivated Plants. November 19-19, Aschersleben, Germany - p. 1 - 3.
    Reply to H-M Cheng and K Sundram
    Katan, M.B. ; Zock, P.L. ; Bovenkamp, P. van de - \ 1999
    American Journal of Clinical Nutrition 70 (1999)1. - ISSN 0002-9165 - p. 104 - 105.
    Infrared Photothermal Spectroscopy in the Science of Human Nutrition
    Bicanic, D. ; Fink, T. ; Franko, M. ; Mocnik, G. ; Bovenkamp, P. van de; Veldhuizen, A. van; Gerkema, E. - \ 1999
    In: Photoacoustic and Photothermal Phenomena / Scudieri, F., Bertolotti, M., Woodburry, New York : American Institute of Physics (American Institute of Physics (AIP) Conference Proceedings 463) - ISBN 9781563968051 - p. 637 - 639.
    Some Laser Based Photothermal Methods and Their Applications for Optical and Thermal Characterisation of Lipids
    Bicanic, D. ; Franko, M. ; Mocnik, G. ; Dadarlat, D. ; Sikovec, M. ; Niederlander, H. ; Bovenkamp, P. van der; Lutteroti, S. ; Gibkes, J. ; Klift, E. van der; Bogoni, P. ; Cerni, L. ; Procedda, G. - \ 1999
    In: Proceedings of 23rd World Congress of the International Society for Fat Research (ISF), October 3-7, 1999, Brighton, England. - Champaign, Illinois, USA : American Oil Chemical Society (AOCS) Press, 1999. - ISBN 1-893997-08-1 - p. 77 - 78.
    Voedingsmiddelenanalyses van de Vakgroep Humane Voeding. Deel 12 Margarines, spijsvetten, koekjes en snacks-1996
    Hulshof, P.J.H. ; Kosmeijer, T. ; Zock, P.L. ; Bovenkamp, P. van de; Katan, M.B. - \ 1998
    Unknown Publisher
    voedingsmiddelen - voedsel - chemische samenstelling - bakoliën - bakvetten - oliën - boter - margarine - voedingswaarde - voedselsamenstelling - kwaliteit - eigenschappen - huishoudens - ziekenhuizen - vetten - ziekenhuiscatering - verpleeghuizen - ingrediënten - dierlijke producten - ghana - filippijnen - spijsoliën - spijsvetten - institutionele huishoudens - foods - food - chemical composition - cooking oils - cooking fats - oils - butter - margarine - nutritive value - food composition - quality - properties - households - hospitals - fats - hospital catering - nursing homes - ingredients - animal products - ghana - philippines - edible oils - edible fats - institutional households
    Daling transvetzuren, maar niet in alle grootverbruikproducten: vetzuursamenstelling van spijsvetten, koek en snacks.
    Hulshof, P.J.M. ; Zock, P.L. ; Kosmeijer-Schuil, J.G. ; Bovenkamp, P. van de; Katan, M.B. - \ 1998
    Voeding 59 (1998)5. - ISSN 0042-7926 - p. 24 - 27.
    vetzuren - carbonzuren - vetten - oliën - bakoliën - bakvetten - zoetwaren - biscuits - fabricage - cakes - snacks - maaltijden - voedingsmiddelen - chemische samenstelling - voeding - oleïnezuur - onverzadigde vetzuren - acrylzuur - volksgezondheid - gezondheidszorg - spijsoliën - spijsvetten - fatty acids - carboxylic acids - fats - oils - cooking oils - cooking fats - confectionery - biscuits - manufacture - cakes - snacks - meals - foods - chemical composition - nutrition - oleic acid - unsaturated fatty acids - acrylic acid - public health - health care - edible oils - edible fats
    Deze vetzuren hebben een ongunstige invloed op het lipoproteineprofiel in het bloed. In een tabel is de vetzuursamenstelling van geselecteerde koek en snacks weergegeven. Tegenwoordig bevatten de margarines, bak- en braadvetten aanzienlijk minder verzadigd vet
    Analysis of C18:1 cis and trans fatty acid isomers by the combination of gas-liquid chromatography of 4,4-dimethyloxazoline derivatives and methylesters.
    Aro, A. ; Kosmeijer-Schuil, J.G. ; Bovenkamp, P. van de; Hulshof, P.J.M. ; Zock, P.L. ; Katan, M.B. - \ 1998
    Journal of the American Oil Chemists Society 75 (1998). - ISSN 0003-021X - p. 977 - 985.
    Determination of trans-beta carotene and other carotenoids in blood plasma using HPLC and thermal lens detection.
    Franko, M. ; Bovenkamp, P. van de; Bicanic, D. - \ 1998
    Journal of chromatography. B, Biomedical sciences and applications 718 (1998). - ISSN 1387-2273 - p. 47 - 54.
    Determination of carotenoids by HPLC-TLS.
    Franko, M. ; Luterotti, S. ; Bovenkamp, P. van de; Bicanic, D. - \ 1998
    In: 5th International Conference Chromatography and Hyphenated Techniques, Bled - p. 133 - 133.
    Application of infrared photothermal spectroscopy in human nutrition.
    Bicanic, D. ; Fink, T. ; Franko, M. ; Bovenkamp, P. van de; Veldhuizen, B. van; Gerkema, E. - \ 1998
    In: 10th International Conference on Photoacoustic and Photothermal Phenomena, Rome, The Netherlands - p. 513 - 514.
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