Financieringsbehoefte natuurinclusieve landbouw : rapportage eerste fase: beschrijvende analyse vragenlijst / Jetske Bouma, Mark Koetse, Nico Polman; met dank aan Jeroen Brandsma : Rapportage eerste fase: beschrijvende analyse vragenlijst / Jetske Bouma, Mark Koetse, Nico Polman; met dank aan Jeroen Brandsma
Bouma, Jetske ; Koetse, Mark ; Polman, Nico ; Brandsma, Jeroen - \ 2019
Den Haag : PBL Planbureau voor de Leefomgeving - 73
The Solanum tuberosum KST1 partial promoter as a tool for guard cell expression in multiple plant species
Kelly, Gilor ; Lugassi, Nitsan ; Belausov, Eduard ; Wolf, Dalia ; Khamaisi, Belal ; Brandsma, Danja ; Kottapalli, Jayaram ; Fidel, Lena ; Ben-Zvi, Batsheva ; Egbaria, Aiman ; Acheampong, Atiako Kwame ; Zheng, Chuanlin ; Or, Etti ; Distelfeld, Assaf ; David-Schwartz, Rakefet ; Carmi, Nir ; Granot, David - \ 2017
Journal of Experimental Botany 68 (2017)11. - ISSN 0022-0957 - p. 2885 - 2897.
Confocal imaging - GFP - guard cell-specific promoters - KST1 promoter - stomata
To date, guard cell promoters have been examined in only a few species, primarily annual dicots. A partial segment of the potato (Solanum tuberosum) KST1 promoter (KST1 partial promoter, KST1 ppro) has previously been shown to confer guard cell expression in potato, tomato (Solanum lycopersicum), citrus [Troyer citrange (C. sinensis×Poncirus trifoliata)], and Arabidopsis (Arabidopsis thaliana). Here, we describe an extensive analysis of the expression pattern of KST1 ppro in eight (previously reported, as well as new) species from five different angiosperm families, including the Solanaceae and the Cucurbitaceae, Arabidopsis, the monocot barley (Hordeum vulgare), and two perennial species: grapevine (Vitis vinifera) and citrus. Using confocal imaging and three-dimensional movies, we demonstrate that KST1 ppro drives guard cell expression in all of these species, making it the first dicot-originated guard cell promoter shown to be active in a monocot and the first promoter reported to confer guard cell expression in barley and cucumber (Cucumis sativus). The results presented here indicate that KST1 ppro can be used to drive constitutive guard cell expression in monocots and dicots and in both annual and perennial plants. In addition, we show that the KST1 ppro is active in guard cells shortly after the symmetric division of the guard mother cell and generates stable expression in mature guard cells. This allows us to follow the spatial and temporal distribution of stomata in cotyledons and true leaves.
Sex impacts Th1 cells, Tregs, and DCs in both intestinal and systemic immunity in a mouse strain and location-dependent manner
Elderman, M. ; Beek, A.A. van; Brandsma, E. ; Haan, B. de; Savelkoul, H.F.J. ; Vos, P. de; Faas, Marijke M. - \ 2016
Biology of Sex Differences 7 (2016). - ISSN 2042-6410
Background Males and females have a different predisposition for the development of intestinal disorders, like inflammatory bowel disease (IBD). We hypothesized that sex specific differences in intestinal immune responses may underlie this bias. To test this hypothesis, we studied sex differences in immune cell populations in the Peyer’s patches (PP). For comparison with systemic immunity, we studied spleen cells. Methods Two mouse strains with different susceptibility for developing colitis (BALB/c and C57Bl/6) were used. Using flow cytometry, we measured the percentage of T cells, Th1, Th17, and Treg cells in the PP and spleen. In addition, we measured the percentages of NK cells, macrophages, myeloid, and lymphoid dendritic cells (DCs) and their expression of CD80 and CD103. Moreover, we measured percentages of monocyte subsets in the peripheral circulation. Results were tested using two-way ANOVA, p < 0.05. Results Males had a lower percentage of T cells in both PP and spleen (PP BALB/c 22.1 %, B6 13.6 %; spleen BALB/c 4.7 %, B6 19.9 %) but a higher percentage of Th1 cell in both tissues (PP BALB/c 350 %, B6 109.5 %; spleen BALB/c 48.7 %, B6 41.9 %) than females. They also had a higher percentage of Tregs in the spleen than females (BALB/c 20.5 %, B6 4.5 %). Furthermore, males had a higher percentage of CD80+ DCs in both the PP and spleen (lymphoid DCs in PP BALB/c 104.7 %, B6 29.6 %; spleen BALB/c 72.2 %, B6 44.2 %; myeloid DCs in PP BALB/c 80.5 %, B6 93.3 %; spleen BALB/c 88.5 %, B6 50.8 %) and a higher percentage of lymphoid CD103+ DCs in the spleen than females (BALB/c 41.5 %, B6 28.3 %). The percentage of NK cells was decreased in the spleen (BALB/c 12.5 %, B6 25.1 %) but increased in the PP (BALB/c 75.7 %, B6 78.6 %) of males as compared with females. Strain differences were also found in the PP; BALB/c mice had a higher percentage of T cells (males 58.1 %, females 75.5 %), a higher Th/Tc ratio (males 81.0 %, females 134.2 %), less FoxP3+CD25− T cells (males 14.6 %, females 30.0 %), more DCs (males 14.8 %, females 15.7 %) and macrophages (males 67.9 %, females 141.2 %), and more NK cells (males 160 %, females164.3 %) than BALB/c mice. Conclusions In this study, we show sex differences in intestinal and peripheral immune populations. These differences may underlie sex differences in intestinal disorders like IBD, and this information may be an important knowledge for the treatment of intestinal-related diseases.
A new Dutch Net Energy formula for feed and feedstuffs for growing and fattening pigs
Blok, M.C. ; Brandsma, Gerard ; Bosch, G. ; Gerrits, W.J.J. ; Jansman, A.J.M. ; Everts, H. - \ 2015
Wageningen UR Livestock Research (CVB-Documentation report 56) - 39
|Aeration reduces stress in Lactococcus lactis MG1363
Brandsma, J.B. ; Wegkamp, A. ; Abee, T. ; Zwietering, M.H. ; Meijer, W. - \ 2014
In: Book of abstracts 11th International Symposium on Lactic Acid Bacteria. - - p. E089 - E089.
The use of starter cultures is an essential aspect in cheese manufacturing. Starter cultures may consist of single strains or mixtures of different strains and species of mainly lactic acid bacteria. An undefined mixture of different strains and species can be regarded as a complex ecosystem. Starter culture “Ur”, used for the production of Gouda cheese is an example of a complex starter culture and it contains 7 genetic lineages of Lactococcus lactis and 1 of Leuconostoc mesenteroides . Complex ecosystems are known for their stability in composition, even during the process of back-slopping, where new milk is inoculated with whey from the previous batch. We set out to investigate the compositional stability of starter culture Ur during propagation at different temperatures. In addition, Ur was reconstituted from the 8 defined genetic lineages in 1:1 starting ratio. Propagation was carried out in fat free milk and the inoculated milk was incubated at 20°C, 25°C and 30°C. These temperatures were selected to possibly favor strains that have different optimum growth temperatures. After 28 transfers (~200 generations) the composition of Ur and the reconstituted mixtures was determined using qPCR with lineage specific primers. Sequential propagation of Ur in milk at different temperatures induced changes in the relative abundance of different genomic lineages, but none of the lineages were lost. We also demonstrated that reconstituted Ur, in comparison with original Ur, leads to a similar end-point composition after 28 transfers as Ur. This demonstrates the robustness of the original Ur culture and suggests interactions between the different lineages. 1. Erkus O, de Jager VC, Spus M, van Alen-Boerrigter IJ, van Rijswijck IM, Hazelwood L, Janssen PW, van Hijum SA, Kleerebezem M, Smid EJ: Multifactorial diversity sustains microbial community stability. ISME J 2013, 7:2126-2136.
Variation in phosphorus content of milk from dairy cattle as affected by differences in milk composition
Klop, G. ; Ellis, J.L. ; Blok, M.C. ; Brandsma, G.G. ; Bannink, A. ; Dijkstra, J. - \ 2014
The Journal of Agricultural Science 152 (2014)5. - ISSN 0021-8596 - p. 860 - 869.
environmental-impact - methane production - blood-plasma - beef-cattle - cows - excretion - prediction - magnesium - calcium
In view of environmental concerns with regard to phosphorus (P) pollution and the expected global P scarcity, there is increasing interest in improving P utilization in dairy cattle. In high-producing dairy cows, P requirements for milk production comprise a significant fraction of total dietary P requirements. Although variation in P content of milk can affect the efficiency of P utilization for milk production (i.e. the fraction of ingested P that is incorporated in milk), this variation is poorly understood. It was hypothesized that the P content of milk is related to both milk protein and milk lactose content, but not necessarily to milk fat content. Three existing experiments comprising individual animal data on milk yield and fat, protein, lactose and P content of milk (in total 278 observations from 121 cows) were analysed to evaluate this hypothesis using a mixed model analysis. The models including the effects of both protein and lactose content of milk yielded better prediction of milk P content in terms of root-mean-square prediction error (RMSPE) and concordance correlation coefficient (CCC) statistics than models with only protein included as prediction variable; however, estimates of effect sizes varied between studies. The inclusion of milk fat content in equations already including protein and lactose did not further improve prediction of milk P content. Equations developed to describe the relationship between milk protein and lactose contents (g/kg) and milk P content (g/kg) were: (Expt 1) P in milk=-0·44(±0·179)+0·0253(±0·00300)×milk protein+0·0133(±0·00382)×milk lactose (RMSPE: 5·2%; CCC: 0·71); (Expt 2) P in milk=-0·26 (±0·347)+0·0174(±0·00328)×milk protein+0·0143 (±0·00611)×milk lactose (RMSPE: 6·3%; CCC: 0·40); and (Expt 3) P in milk=-0·36(±0·255)+0·0131(±0·00230)×milk protein+0·0193(±0·00490)×milk lactose (RMSPE: 6·5%; CCC: 0·55). Analysis of the three experiments combined, treating study as a random effect, resulted in the following equation to describe the same relationship as in the individual study equations: P in milk=-0·64(±0·168)+0·0223(±0·00236)×milk protein+0·0191(±0·00316)×milk lactose (RMSPE: 6·2%; CCC: 0·61). Although significant relationships between milk protein, milk lactose and milk P were found, a considerable portion of the observed variation remained unexplained, implying that factors other than milk composition may affect the P content of milk. The equations developed may be used to replace current fixed milk P contents assumed in P requirement systems for cattle.
Are major economies on track to achieve their pledges for 2020? An assessment of domestic climate and energy policies
Roelfsema, M. ; Elzen, M.G.J. den; Hoehne, N.E. ; Hof, A.F. ; Braun, N. ; Fekete, H. ; Böttcher, H. ; Brandsma, R. ; Larkin, J. - \ 2014
Energy Policy 67 (2014). - ISSN 0301-4215 - p. 781 - 796.
gas emission reductions - annex i countries
Many of the major greenhouse gas emitting countries have planned and/or implemented domestic mitigation policies, such as carbon taxes, feed-in tariffs, or standards. This study analyses whether the most effective national climate and energy policies are sufficient to stay on track for meeting the emission reduction proposals (pledges) that countries made for 2020. The analysis shows that domestic policies of India, China and Russia are projected to lead to lower emission levels than the pledged levels. Australia's and the EU's nationally legally binding policy framework is likely to deliver their unconditional pledges, but not the conditional ones. The situation is rather unclear for Japan, South Korea, Brazil and Indonesia. We project that policies of Canada and the USA will reduce 2020 emission levels, but additional policies are probably needed to deliver their pledges in full. The analysis also shows that countries are implementing policies or targets in various areas to a varying degree: all major countries have set renewable energy targets; many have recently implemented efficiency standards for cars, and new emission trading systems are emerging.
The major Ethiopian milksheds : an assessment of development potential
Brandsma, W. ; Mengistu, D. ; Kassa, B. ; Yohannes, M. ; Lee, J. van der - \ 2013
Lelystad : Wageningen UR Livestock Research (Livestock Research report 735) - 237
dairy farming - agricultural development - value chain analysis - ethiopia - melkveehouderij - landbouwontwikkeling - waardeketenanalyse - ethiopië
The development potential of 8 milksheds in Ethiopia was assessed and ranked for potential for value chain development, as part of the MIDD project. Major criteria for ranking were market potential, production potential, presence of services, and commercialization level of the value chain. Highest ranking milk sheds were Addis Abeba, Adama-Asella, and Bahir Dar–Gondar milksheds.
Report on pilot large scale farm advice
Wouters, A.P. ; Brandsma, W. ; Tessema, A. - \ 2013
Lelystad : Wageningen UR Livestock Research (Report / Wageningen UR Livestock Research 732) - 23
|B-cells in ageing
Beek, A.A. van; Brandsma, E. ; Elderman, M. ; Faassen, M. ; Vos, P. de; Wichers, H.J. ; Savelkoul, H.F.J. - \ 2013
Organophosphorus flame-retardant and plasticizer analysis, including recommendations form the first worldwide interlaboratory study
Brandsma, S.H. ; Boer, J. de; Leonards, P.E.G. ; Cofino, W.P. ; Covaci, A. - \ 2013
TrAC : Trends in Analytical Chemistry 43 (2013). - ISSN 0165-9936 - p. 217 - 228.
tandem mass-spectrometry - indoor environments - gas-chromatography - population characteristics - chemical-ionization - organic-compounds - phthalate-esters - water samples - new-model - air
The first worldwide interlaboratory study on organophosphorus flame retardants (PFRs) was organized to improve the quality of the data reported in the literature. The study involved standard solutions, dust, fish oil and sediment samples. The differences in coefficients of variation (CV) between the samples were related more to PFR concentration (with high blanks being reported by some laboratories) and less to matrix type. Not all participating laboratories suffered from blank problems, which indicated that it was possible to control the blanks. We include recommendations on how to improve analytical performance, especially to reduce contamination of blanks.
Optimalisatie van het basismeetnet van het Landelijk Meetnet effecten Mestbeleid : studie naar bezuinigingsmogelijkheden
Fraters, B. ; Beijen, B.A. ; Brandsma, G.J. ; Rijswick, H.F.M.W. ; Reijs, J.W. ; Buis, E. ; Hoogeveen, M.W. - \ 2012
Bilthoven : Rijksinstituut voor Volksgezondheid en Milieu - 29
monitoring - mestbeleid - waterverontreiniging - nitraatreductie - emissie - eu regelingen - monitoring - manure policy - water pollution - nitrate reduction - emission - eu regulations
Door te besparen op de uitgaven voor het basismeetnet van het Landelijk Meetnet effecten Mestbeleid (LMM) kan mogelijk niet aan de Europese rapportageverplichting worden voldaan. Door bezuinigingen kunnen namelijk de ontwikkelingen in de nitraatconcentratie in water op lange termijn onvoldoende in beeld worden gebracht. Hetzelfde geldt voor de relatie tussen de nitraatconcentratie en de bedrijfsvoering op landbouwbedrijven. Hierdoor wordt minder inzicht verkregen in de effecten van de landbouwpraktijk, en ontwikkelingen daarin, op de waterkwaliteit.
Arginine metabolism in sugar deprived Lactococcus lactis enhances survival and cellular activity, while supporting flavour production
Brandsma, J.B. ; Kraats, I. van de; Abee, T. ; Zwietering, M.H. ; Meijer, W.C. - \ 2012
Food Microbiology 29 (2012)1. - ISSN 0740-0020 - p. 27 - 32.
amino-acid catabolism - aroma compounds - lactobacillus-helveticus - carbohydrate starvation - dehydrogenase-activity - alpha-ketoglutarate - semihard cheese - bacteria - conversion - aminotransferases
Flavour development in cheese is affected by the integrity of Lactococcus lactis cells. Disintegrated cells enhance for instance the enzymatic degradation of casein to free amino acids, while integer cells are needed to produce specific flavour compounds from amino acids. The impact of the cellular activity of these integer cells on flavour production remains to be elucidated. In this study we investigated whether lactose-deprived L. lactis cells that use arginine as an alternative energy source can extend cellular activity and produce more specific flavours. In cheese experiments we demonstrated that arginine metabolising cells survived about 3 times longer than non-arginine metabolising cells, which suggests prolonged cellular activity. Cellular activity and flavour production of L. lactis was further studied in vitro to enable controlled arginine supplementation. Comparable with the results found in cheese, the survival rates of in vitro incubated cells improved when arginine was metabolised. Furthermore, elongated cellular activity was reflected in 3–4-fold increased activity of flavour generating enzymes. The observed prolonged cellular activity resulted in about 2-fold higher concentrations of typical Gouda cheese flavours. These findings provide new leads for composing starter cultures that will produce specific flavour compounds
|Eerst 150 volt, daarna een pekelbad ( interview met M. de Graaf)
Brandsma, J. ; Graaf, M. de - \ 2011
Trouw (2011). - 1 p.
Towards an Ecological Society: How Landscape Architecture can contribute to a Cultural Transition
Brandsma, S.H. ; Kupers, P.I.M. ; Oudes, H.H. - \ 2010
In: European Council of Landscape Architects Schools 27th ECLAS Conference, Istanbul, Turkey, 29 September - 2 October 2010. - Istanbul : Istanbul Technical University - p. 39 - 49.
|Beperking vangst heeft effect: haringstand weer gegroeid (interview M. Dickey-Collas)
Brandsma, S.H. ; Dickey-Collas, M. - \ 2010
Trouw (2010). - 1 p.
Identification of Hydroxylated Metabolites of Hexabromocyclododecane in Wildlife and 28-days Exposed Wistar Rats
Brandsma, S.H. ; Ven, L.T.M. van der; Boer, J. de; Leonards, P.E.G. - \ 2009
Environmental Science and Technology 43 (2009)15. - ISSN 0013-936X - p. 6058 - 6063.
mass-spectrometry - thermal-stability - products - fish
We studied the presence of hydroxylated metabolites of hexabromocyclododecane (HBCD) in three wildlife species (tern egg, seal, and flounder) and in Wistar rats exposed to 30 and 100 mg HBCD/kg bw/day for 28 days. A nondestructive extraction, fractionation, and cleanup method was developed to separate the hydroxylated HBCD metabolites from the biotic sample matrix. Four different groups of hydroxylated HBCD metabolites were identified in rat adipose, liver, lung, and muscle tissues by liquid and gas chromatography (LC and GC) combined with mass spectrometry (MS): monohydroxy metabolites of HBCD, pentabromocyclododecene (PBCDe), tetrabromocyclododecene (TBCDe), and dihydroxy-HBCD. Dihydroxy-PBCDe was identified by GC-MS but could not be confirmed by LC-MS. Debromination of HBCD to PBCDe was another metabolic pathway observed. In tern eggs from the Western Scheldt the monohydroxy-HBCD was found and in the blubber of harbor seal (Wadden Sea) the monohydroxy metabolites of HBCD and PBCDe were found. No hydroxylated metabolites were detected in the tissue of flounder (Wadden Sea). To our knowledge, this is the first study to identify different hydroxylated metabolite groups of HBCD in rat and wildlife samples.
Improving animal health systems by integrating public and private interests
Brandsma, J. ; Bos, A.P. ; Napel, J. ten; Peet, G.F.V. van der - \ 2008
dierhouderij - diergezondheid - dierverzorging - animal husbandry - animal health - care of animals
Folder over hoe private en publieke gezondheidszorg voor dieren verenigd kunnen worden
|Penssynchronisatie: toetsing in voederproeven
Duinkerken, G. van; Bakker, J. ; Blok, M.C. ; Brandsma, G.G. ; Cone, J.W. - \ 2008
Wageningen : Animal Sciences Group (Rapport / Animal Sciences Group 30) - 75 p.
Eiwitnotering vers gras onderzocht; DVE lager en PEB hoger
Klop, A. ; Brandsma, G.G. - \ 2008
V-focus 5 (2008)2. - ISSN 1574-1575 - p. 14 - 15.
rundveehouderij - weiden - begrazing - voedering - grassen - eiwitwaarde - voedingswaarde - waardesystemen - cattle husbandry - pastures - grazing - feeding - grasses - protein value - nutritive value - value systems
In dit artikel meer over de eiwitwaarde van vers gras, die in het DVE/OEB-2007 systeem een andere waardering krijgt