Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Records 1 - 20 / 37

  • help
  • print

    Print search results

  • export

    Export search results

  • alert
    We will mail you new results for this query: q=Brandwagt
Check title to add to marked list
Functional analysis of Avr2 and Avr4 proteins of Cladosporium fulvum
Wit, P.J.G.M. de; Brandwagt, B.F. ; Bolton, M.D. ; Burg, H.A. van den; Esse, H.P. van; Fradin, E.F. ; Gabriëls, S.H.E.J. ; Ellendorff, U. ; Hoorn, R.A.L. van der; Jones, J.D.G. ; Joosten, M.H.A.J. ; Klooster, J.W. van t; Kock, M.J.D. de; Kruijt, M. ; Lindhout, P. ; Rooney, H.C.E. ; Stergiopoulos, I. ; Stulemeijer, I.J.E. ; Vervoort, J.J.M. ; Vossen, J.H. ; Thomma, B.P.H.J. - \ 2006
In: Biology of Plant-Microbe Interactions / Sánchez, F., Quinto, C., López-Lara, I.M., Geiger, O., St. Paul, USA : IS MPMI - ISBN 0965462544 - p. 35 - 41.
The Cf-4 and Cf-9 resistance genes against Cladosporium fulvum are conserved in wild tomato species
Kruijt, M. ; Kip, D.J. ; Joosten, M.H.A.J. ; Brandwagt, B.F. ; Wit, P.J.G.M. de - \ 2005
Molecular Plant-Microbe Interactions 18 (2005)9. - ISSN 0894-0282 - p. 1011 - 1021.
plant-disease-resistance - leucine-rich repeat - avirulence protein avr4 - race-specific elicitor - genus lycopersicon - hypersensitive response - arabidopsis-thaliana - balancing selection - locus comprises - recognition
The Cf-4 and Cf-9 genes originate from the wild tomato species Lycopersicon hirsutum and L. pimpinellifolium and confer resistance to strains of the leaf mold fungus Cladosporium fulvum that secrete the Avr4 and Avr9 elicitor proteins, respectively. Homologs of Cf-4 and Cf-9 (Hcr9s) are located in several clusters and evolve mainly through sequence exchange between homologs. To study the evolution of Cf genes, we set out to identify functional Hcr9s that mediate recognition of Avr4 and Avr9 (designated Hcr9-Avr4s and Hcr9-Avr9s) in all wild tomato species. Plants responsive to the Avr4 and Avr9 elicitor proteins were identified throughout the genus Lycopersicon. Open reading frames of Hcr9s from Avr4- and Avr9-responsive tomato plants were polymerase chain reaction-amplified. Several Hcr9s that mediate Avr4 or Avr9 recognition were identified in diverged tomato species by agroinfiltration assays. These Hcr9-Avr4s and Hcr9-Avr9s are highly identical to Cf-4 and Cf-9, respectively. Therefore, we conclude that both Cf-4 and Cf-9 predate Lycopersicon speciation. These results further suggest that C fulvum is an ancient pathogen of the genus Lycopersicon, in which Cf-4 and Cf-9 have been maintained by selection pressure imposed by C fulvum.
Functional analysis of Avr2 and Avr4 of Cladosporium fulvum
Wit, P.J.G.M. de; Brandwagt, B. ; Burg, H. van den; Bolton, M. ; Gabriëls, S. ; Kock, M. de; Hoorn, R. van der; Klooster, J. van t; Kruijt, M. ; Stulemeijer, I. ; Vossen, J. ; Berg-Velthuis, G. van den; Esse, P. van; Jones, J.D.G. ; Rooney, H.C.E. ; Joosten, M. ; Thomma, B. - \ 2005
In: Book of Abstracts XII International Congress on Molecular Plant-Microbe Interactions, Mérida, México, 14-19 December 2005 - p. 36 - 37.
The tomato Orion locus comprises a unique class of Hcr9 genes
Kock, M.J.D. de; Brandwagt, B.F. ; Bonnema, A.B. ; Wit, P.J.G.M. de; Lindhout, P. - \ 2005
Molecular Breeding 15 (2005)4. - ISSN 1380-3743 - p. 409 - 422.
disease resistance gene - brassinosteroid signal-transduction - cladosporium-fulvum - short arm - mapping strategy - 26s proteasome - cf-genes - chromosome-1 - proteins - repeat
Resistance against the tomato fungal pathogen Cladosporium fulvum is often conferred by Hcr9 genes (Homologues of the C. fulvum resistance gene Cf-9) that are located in the Milky Way cluster on the short arm of chromosome 1. These Hcr9 genes mediate recognition of fungal avirulence gene products. In contrast, the resistance gene Cf-Ecp2 mediates recognition of the virulence factor Ecp2 and is located in the Orion (OR) cluster on the short arm of chromosome 1. Here, we report the map- and homology-based cloning of the OR Hcr9 cluster. A method was optimised to generate clone-specific fingerprint data that were subsequently used in the efficient calculation of genomic DNA contigs. Three Hcr9s were identified as candidate Cf-Ecp2 genes. By PCR-based cloning using specific OR sequences, orthologous Hcr9 genes were identified from different Lycopersicon species and haplotypes. The OR Hcr9s are very homologous. However, based on the relative low sequence homology to other Hcr9s, the OR Hcr9s are classified as a new subgroup
Molecular evolution of Cladosporium fulvum disease resistance genes in wild tomato
Kruijt, M. - \ 2004
Wageningen University. Promotor(en): Pierre de Wit, co-promotor(en): B.F. Brandwagt. - [S.l.] : S.n. - ISBN 9085040817 - 136
solanum - tomaten - wilde verwanten - genen - ziekteresistentie - genetische variatie - evolutie - solanum - tomatoes - wild relatives - genes - disease resistance - genetic variation - evolution
Micro-evolution of (a)virulence genes of Cladosporium fulvum
Wit, P.J.G.M. de; Brandwagt, B.F. ; Burg, H.A. van den; Gabriëls, S.H.E.J. ; Kock, M.J.D. de; Hoorn, R.A.L. van der; Klooster, J.W. van t; Kruijt, M. ; Stulemeijer, I.J.E. ; Vossen, J.H. ; Berg-Velthuis, G.C.M. van den; Clergeot, P.J.M. ; Esse, H.P. van; Vervoort, J.J.M. ; Lindhout, W.H. ; Joosten, M.H.A.J. ; Thomma, B.P.H.J. - \ 2004
In: Book of Abstracts CBS Centenary Symposium, Amsterdam, the Netherlands, 13-14 May 2004 - p. 11 - 11.
Fungal (a)virulence genes and plant resistance genes; a rat race between Cladosporium fulvum and its host plant tomato
Wit, P.J.G.M. de; Brandwagt, B.F. ; Burg, H.A. van den; Gabriëls, S.H.E.J. ; Kock, M.J.D. de; Hoorn, R.A.L. van der; Klooster, J.W. van t; Kruijt, M. ; Stulemeijer, I.J.E. ; Vossen, J.H. ; Berg-Velthuis, G.C.M. van den; Clergeot, P.J.M. ; Esse, H.P. van; Vervoort, J.J.M. ; Lindhout, W.H. ; Joosten, M.H.A.J. ; Thomma, B.P.H.J. - \ 2004
In: Book of Abstracts Plant Genomics European Meetings, Lyon, France, 22-25 September 2004 - p. 44 - 44.
Molecular basis of plant response to microbial invasion
Wit, P.J.G.M. de; Brandwagt, B.F. ; Burg, H.A. van den; Gabriëls, S.H.E.J. ; Hoorn, R.A.L. van der; Jong, C.F. de; Klooster, J.W. van t; Kock, M.J.D. de; Kruijt, M. ; Luderer, R. ; Munnik, T. ; Stulemeijer, I.J.E. ; Thomma, B.P.H.J. ; Vervoort, J.J.M. ; Westerink, N. ; Joosten, M.H.A.J. - \ 2004
In: Proceedings of the 11th International Congress on Molecular Plant-Microbe Interactions, St.-Petersburg, Russia, 18-26 July 2003 / Tikhonovich, I., Lugtenberg, B., Provorov, N., St. Paul, Minnesota, USA : International Society for Molecular Plant-Microbe Interactions - ISBN 0965462536 - p. 203 - 207.
Rearrangements in the Cf-9 disease resistance gene cluster of wild tomato have resulted in three genes that mediate Avr9 responsiveness
Kruijt, M. ; Brandwagt, B.F. ; Wit, P.J.G.M. de - \ 2004
Genetics 168 (2004). - ISSN 0016-6731 - p. 1655 - 1663.
cladosporium-fulvum - lycopersicon-pimpinellifolium - plant-resistance - proteins - evolution - recognition - recombination - chromosome-1 - virulence - locus
Cf resistance genes in tomato confer resistance to the fungal leaf pathogen Cladosporium fulvum. Both the well-characterized resistance gene Cf-9 and the related 9DC gene confer resistance to strains of C. fulvum that secrete the Avr9 protein and originate front the wild tomato species Lycopersicon pimpinellifolium. We show that 9DC and Cf-9 are allelic, and we have isolated and sequenced the complete 9DC Cluster of L. pimpinellifolium LA1301. This 9DC cluster harbors five full-length Cf homologs, including orthologs of the most distal homologs; of the Cf-9 Cluster and three central 9DC genes. Two 9DC genes (9DC1 and 9DC2) have an identical coding sequence, whereas 9DC3 differs at its 3' terminus. From a detailed comparison of the 9DC and Cf-9 clusters, We conclude that the Cf-9 and Hcr9-9D genes front the Cf-9 cluster are ancestral to the first 9DC gene and that the three 9DC genes were generated by subsequent intra- and intergenic unequal recombination events. Thus, the 9DC cluster has undergone substantial rearrangements in the central region, but riot at the ends. Using transient transformation assays, we show that all three 9DC genes confer Avr9 responsiveness, but that 9DC2 is likely the main determinant of Avr9 recognition in LA1301.
Cladosporium fulvum circumvents the second functional resistance gene homologue at the Cf-4 locus (Hcr9-4E) by secretion of a stable avr4E isoform
Westerink, N. ; Brandwagt, B.F. ; Wit, P.J.G.M. de; Joosten, M.H.A.J. - \ 2004
Molecular Microbiology 54 (2004)2. - ISSN 0950-382X - p. 533 - 545.
fungal tomato pathogen - potato-virus-x - avirulence protein avr4 - hygromycin-b resistance - disease resistance - elicitor proteins - expression - identification - plants - recognition
Introgression of resistance trait Cf-4 from wild tomato species into tomato cultivar MoneyMaker (MM-Cf0) has resulted in the near-isogenic line MM-Cf4 that confers resistance to the fungal tomato pathogen Cladosporium fulvum. At the Cf-4 locus, five homologues of Cladosporium resistance gene Cf-9 (Hcr9s) are present. While Hcr9-4D represents the functional Cf-4 resistance gene matching Avr4, Hcr9-4E confers resistance towards C. fulvum by mediating recognition of the novel avirulence determinant Avr4E. Here, we report the isolation of the Avr4E gene, which encodes a cysteine-rich protein of 101 amino acids that is secreted by C. fulvum during colonization of the apoplastic space of tomato leaves. By complementation we show that Avr4E confers avirulence to strains of C. fulvum that are normally virulent on Hcr9-4E-transgenic plants, indicating that Avr4E is a genuine, race-specific avirulence determinant. Strains of C. fulvum evade Hcr9-4E-mediated resistance either by a deletion of the Avr4E gene or by production of a stable Avr4E mutant protein that carries two amino acid substitutions, Phe(82)Leu and Met(93)Thr. Moreover, we demonstrate by site-directed mutagenesis that the single amino acid substitution Phe(82)Leu in Avr4E is sufficient to evade Hcr9-4E-mediated resistance.
Recognition of Cladosporium fulvum Ecp2 elicitor by non-host Nicotiana spp. is mediated by a single dominant gene that is not homologous to known Cf-genes
Kock, M.J.D. de; Iskandar, H.M. ; Brandwagt, B.F. ; Laugé, R. ; Wit, P.J.G.M. de; Lindhout, W.H. - \ 2004
Molecular Plant Pathology 5 (2004)5. - ISSN 1464-6722 - p. 397 - 408.
cultured parsley cells - tobacco mosaic-virus - f-sp lycopersici - disease resistance - confers resistance - short arm - phytophthora-infestans - oligopeptide elicitor - pathogen phytophthora - functional expression
Cladosporium fulvum is a fungal pathogen of tomato that grows exclusively in the intercellular spaces of leaves. Ecp2 is one of the elicitor proteins that is secreted by C. fulvum and is specifically recognized by tomato plants containing the resistance gene Cf-Ecp2. Recognition is followed by a hypersensitive response (HR) resulting in resistance. HR-associated recognition of Ecp2 has been observed in Nicotiana paniculata, N. sylvestris, N. tabacum and N. undulata that are non-host plants of C. fulvum. Absence of Ecp2-recognition did not lead to growth of C. fulvum on Nicotiana plants. We show that HR-associated recognition of Ecp2 is mediated by a single dominant gene in N. paniculata. However, based on PCR and hybridization analysis this gene is not homologous to known Cf-genes
De moleculaire evolutie van Cf-resistentiegenen in tomaat tegen Cladosporium fulvum
Kruijt, M. ; Brandwagt, B.F. ; Wit, P.J.G.M. de - \ 2004
Gewasbescherming 35 (2004)2. - ISSN 0166-6495 - p. 101 - 101.
Fungal (a)virulence genes; evolution at a micro-scale
Wit, P.J.G.M. de; Brandwagt, B.F. ; Burg, H.A. van den; Gabriëls, S.H.E.J. ; Kock, M.J.D. de; Hoorn, R.A.L. van der; Klooster, J.W. van t; Kruijt, M. ; Stulemeijer, I.J.E. ; Vossen, J.H. ; Berg-Velthuis, G.C.M. van den; Clergeot, P.J.M. ; Esse, H.P. van; Vervoort, J.J.M. ; Lindhout, W.H. ; Joosten, M.H.A.J. ; Thomma, B.P.H.J. - \ 2004
In: Book of Abstracts 7th European Conference on Fungal Genetics, Copenhagen, Denmark, 17-20 April 2004 - p. 40 - 40.
Recognition of the Cladosporium fulvum Ecp2 elicitor in tomato and non-host plants
Kock, M.J.D. de - \ 2004
Wageningen University. Promotor(en): Pierre de Wit, co-promotor(en): Pim Lindhout; B.F. Brandwagt. - Wageningen : S.n. - ISBN 9085040825 - 150
solanum lycopersicum - tomaten - passalora fulva - ziekteresistentie - resistentiemechanismen - antischimmeleigenschappen - pathogenesis-gerelateerde eiwitten - genexpressie - solanum lycopersicum - tomatoes - passalora fulva - disease resistance - resistance mechanisms - antifungal properties - pathogenesis-related proteins - gene expression
Gene-for-gene recognition in the Cladosporium fulvum-tomato interaction
Wit, P.J.G.M. de; Brandwagt, B.F. ; Burg, H.A. van den; Gabriëls, S.H.E.J. ; Kock, M.J.D. de; Hoorn, R.A.L. van der; Jong, C.F. de; Klooster, J.W. van t; Kruijt, M. ; Luderer, R. ; Westerink, N. ; Joosten, M.H.A.J. - \ 2003
Journal of plant pathology - Formerly Rivista di patologia vegetale 85 (2003)4. - ISSN 1125-4653 - p. 275 - 275.
Molecular basis of plant response to microbial invasion
Wit, P.J.G.M. de; Brandwagt, B.F. ; Burg, H.A. van den; Gabriëls, S.H.E.J. ; Kock, M.J.D. de; Hoorn, R.A.L. van der; Jong, C.F. de; Klooster, J.W. van t; Kruijt, M. ; Westerink, N. ; Luderer, R. ; Vervoort, J.J.M. ; Lindhout, W.H. ; Joosten, M.H.A.J. - \ 2003
In: Volume of Abstracts: 11-th International Congress on Molecular Plant-Microbe Interactions, St.-Petersburg, Russia, 18-26 July 2003 St.-Petersburg, Russia : All-Russia Research Institute for Agricultural Microbiology - p. 19 - 19.
Gene-for-gene recognition in the Cladosporium fulvum-tomato interaction
Wit, P.J.G.M. de; Brandwagt, B.F. ; Burg, H.A. van den; Gabriëls, S.H.E.J. ; Kock, M.J.D. de; Hoorn, R.A.L. van der; Jong, C.F. de; Klooster, J.W. van t; Kruijt, M. ; Luderer, R. ; Westerink, N. ; Joosten, M.H.A.J. - \ 2003
In: Book of Abstracts of Invited Papers 8th International Congress of Plant Pathology, Christchurch, New Zealand, 2-7 February 2003 [S.l.] : S.n. - ISBN 086476152X - p. 77 - 77.
Effects on virulence of the tomato pathogen Cladosporium fulvum by RNAi-mediated silencing of avirulence genes
Brandwagt, B.F. ; Kock, M.J.D. de; Joosten, M.H.A.J. ; Wit, P.J.G.M. de - \ 2003
In: Book of Abstracts XXII Fungal Genetics Conference, Asilomar, USA, 18-23 March 2003 Asilomar, USA : S.n. - p. 122 - 122.
The molecular basis of co-evolution between Cladosporium fulvum and tomato
Wit, P.J.G.M. de; Brandwagt, B.F. ; Burg, H.A. van den; Cai, X. ; Hoorn, R.A.L. van der; Jong, C.F. de; Klooster, J. van 't; Kock, M.J.D. de; Kruijt, M. ; Lindhout, W.H. ; Luderer, R. ; Takken, F.L.W. ; Westerink, N. ; Vervoort, J.J.M. ; Joosten, M.H.A.J. - \ 2002
Antonie van Leeuwenhoek: : Nederlandsch tijdschrift voor hygiëne, microbiologie en serologie 81 (2002)1-4. - ISSN 0003-6072 - p. 409 - 412.
cladosporium - plantenziekteverwekkers - solanum - genetisch bepaalde resistentie - overgevoeligheid - plant pathogens - genetic resistance - hypersensitivity
Cladosporium fulvum is a semi-biotrophic pathogen, which causes leaf mold of tomato (Lycopersicon spp.). In our laboratory this pathosystem serves as a model to study gene-for-gene interactions between plants and pathogenic fungi (Joosten & De Wit 1999). Many avirulence (Avr) genes and matching resistance (Cf) genes have been cloned and we are now beginning to understand how their products can induce an array of plant defense responses, including the classic hypersensitive response (HR). Here, we will discuss the latest results of our molecular studies on this interaction. These include the isolation of: (i) two new Avr genes, Avr2 and Avr4E, (ii) the determination of the specificity determinants within the Cf-4 and Cf-9 genes by artificial domain swaps and introduction of point mutations, (iii) the analysis of polymorphism occurring in AVR9-responsive Cf genes occurring in natural populations of L. pimpinellifolium, and finally (iv) the description of an efficient method to identify early HR-related genes.
Resistance of plants to the fungal pathogen Alternaria alternata f.sp. lycopersici
Brandwagt, B.F. - \ 2002
Gewasbescherming 33 (2002)4. - ISSN 0166-6495 - p. 132 - 134.
Check title to add to marked list
<< previous | next >>

Show 20 50 100 records per page

 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.