Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

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    Opportunities for seaweed biorefinery
    Lange, Lene ; Grandorf Bak, Urd ; Cole Brandstrup Hansen, Steffen ; Gregersen, Olavur ; Harmsen, Paulien ; Nordberg Karlsson, Eva ; Meyer, Anne ; Mikkelsen, Maria D. ; Broek, Ben van den; Óli Hreggviðsson, Guðmundur - \ 2020
    In: Sustainable Seaweed Technologies / Dolores Torres, M., Kraan, S., Dominguez, H., Elsevier (Advances in Green Chemistry ) - ISBN 9780128179437 - p. 3 - 31.
    This introductory chapter provides an overview of seaweed biorefinery opportunities, providing basis for multiple value chains, contributing to nutrition and health of a growing global population, to local job generation and development, to ecosystem services, and not the least to climate change mitigation and adaptation. A unique and rich diversity of the seaweed components provides the basis for the broad spectrum of value-chains described here. Red, brown, and green seaweeds are phylogenetically very different and this is reflected in their differences in growth, structure, and biochemical composition. Stable supply and high quality of feedstock are essential for unlocking the value-adding potential of seaweeds. A special focus of the chapter is to provide an overview of the range of different methods of seaweed production (through cultivation or from natural growth, collected or cut at the shore). Furthermore, the results of dedicated efforts to develop new deep-sea cultivation technologies of brown seaweed are highlighted. The chapter has a dual message with regard to seaweed processing: the need to develop more environmentally benign biological processing (to replace chemical processing); the advantage (regarding resource efficiency) and opportunities (social and economic) of designing seaweed biorefineries according to the cascading principle. Making optimized use of all valuable components of seaweed biomass, cascading from high-value products, such as skin care, health-promoting food and feed supplements and functional food ingredients; to lower-value products, such as plant stimulants, soil improvers, and bioenergy. Lastly, this introductory chapter provides global perspectives for future development of sustainable seaweed utilization, contributing to the UN-SDGs, providing livelihood and health for more.
    Stadsklimaat en adaptatie
    Lenzholzer, Sanda ; Antunes Granadeiro Cortesão, João ; Barendrecht, Joost ; Broek, Harold van den - \ 2020
    Urban Climate - biodiversity
    lezing en organisatie van interactieve online sessie
    Progress Report Integrated Seed Sector Development Myanmar: 2019
    Subedi, A. ; Myint, Swe Zin ; Mulerrins, J.L. ; Khin, Thuzar ; Thijssen, M.H. ; Broek, J.A. van den - \ 2020
    - 16 p.
    This report can also be downloaded from :
    Final report to RVO : institutional mapping & needs assessment of Ethiopia’s public seed sector services
    Hassena, Mohammed ; Broek, Joep van den; Borman, Gareth - \ 2020
    Wageningen : Wageningen Centre for Development Innovation (Report / Wageningen Centre for Development Innovation WCDI-20-101) - 29
    In its attempt to transform agriculture, the Ethiopian Ministry of Agriculture (MoA) focuses on increasing agricultural productivity, ensuring food and nutrition security, while promoting exports and import substitution in a sustainable manner. The Netherlands and Ethiopia collaborate to achieve, a.o. by a joint approach to transforming the Ethiopian seed sector. This project provides the basic analysis and recommendations for future activities to establish an efficient, well-regulated and dynamic seed sector that meets quality standards, adapts to climate change and market conditions, has transparent and inclusive governance and maintains biodiversity. The study focuses particularly on the needs of federal and regional seed authorities in terms of technical know-how, institutional capacity and mandates, skills, knowledge and resources.
    Scoping study for a seed laws toolbox
    Broek, Joep van den; Subedi, Abishkar ; Thijssen, Marja H. - \ 2020
    Wageningen : Wageningen Centre for Development Innovation (Report / Wageningen Centre for Development Innovation WCDI-20-100) - 53
    Selective fractionation of free glucose and starch from microalgae using aqueous two-phase systems
    Suarez Ruiz, Catalina A. ; Zarate Baca, Santiago ; Broek, Lambertus A.M. van den; Berg, Corjan van den; Wijffels, Rene H. ; Eppink, Michel H.M. - \ 2020
    Algal Research 46 (2020). - ISSN 2211-9264
    Aqueous two-phase systems - Carbohydrates - Extraction - Glucose - Ionic liquids - Microalgae

    Microalgae are a promising source of lipids, pigments, proteins and carbohydrates, which are valuable compounds for many industries. However, optimal fractionation and valorization of all produced compounds is necessary to improve the economic viability of microalgae production. This paper aims to understand the fractionation of microalgae carbohydrates (free glucose and starch) in aqueous two-phase systems. Three aqueous two-phase systems were investigated to efficiently and mildly separate carbohydrates from disrupted Neochloris oleoabundans. This strain contains 16 w/w% of proteins, 48 w/w% total fatty acids and 27 w/w% carbohydrates when cultivated under saline water and nitrogen depletion conditions. The protein content decreases and the amount of fatty acids and carbohydrates increases notably under stress conditions and glucose becomes the main carbohydrate in this microalgae. Glucose is present in the disrupted microalgae as part of polymeric carbohydrates (starch) or in monomeric form (free glucose). With the aqueous two-phase system Polyethylene Glycol 400 - Cholinium dihydrogen phosphate (PEG400-ChDHp) microalgal free glucose is fractionated up to a recovery of 99% to the most hydrated bottom phase in a single step. Simultaneously, a recovery of 70% is reached for microalgal starch in the interface after two additional liquid-liquid extractions with PEG400-ChDHp. The final fractions obtained were free of pigments.

    Effect of growth conditions on the efficiency of cell disruption of Neochloris oleoabundans
    Safi, C. ; Olivieri, G. ; Engelen-Smit, N. ; Spekking, W. ; Veloo, R. ; Broek, L.A.M. van den; Sijtsma, L. - \ 2020
    Bioresource Technology 300 (2020). - ISSN 0960-8524
    Cell disintegration - Enzymes - High-pressure homogenization - Nitrogen deplete - Nitrogen replete

    The impact of four different growth conditions on the cell disruption efficiency of Neochloris oleoabundans was investigated. A mechanical and biological cell disruption methods were evaluated separately and combined. It has been established that microalgae grown in marine water under nitrogen deprivation were the most resistant against cell disruption methods and released the lowest amount of proteins. The release of lipids, however, followed the “hindered molecule diffusion phenomenon” because it did not follow the same release pattern as proteins. The enzymatic treatment was efficient enough to release the majority of the proteins without combining it with high-pressure homogenization. Regarding energy input, Neochloris oleoabundans grown in marine water under nitrogen deprivation required the highest energy input to release proteins (Ep = 13.76−1) and to break the cells by high-pressure homogenization (Ex – HPH = 1.14−1) or by the combination of enzymes and High-pressure homogenization (Ex – ENZ = 2.79−1).

    Portioning meat and vegetables in four different out of home settings : A win-win for guests, chefs and the planet
    Reinders, Machiel J. ; Lieshout, Lilou van; Pot, Gerda K. ; Neufingerl, Nicole ; Broek, Eva van den; Battjes-Fries, Marieke ; Heijnen, Joris - \ 2020
    Appetite 147 (2020). - ISSN 0195-6663
    Environmental impact - Healthy diet - Meat - Plant-forward diet - Portion size - Real-life intervention study - Restaurant - Vegetables

    Individuals increasingly consume their meals away from home. This article describes a series of studies that examined the effects of meals with reduced amounts of meat and increased amounts of vegetables on food consumption, waste and guest satisfaction in four real-life restaurant settings in the Netherlands: an a-la-carte restaurant, six company canteens, a self-service restaurant, and a buffet restaurant, including nearly 1500 participants in total. The four studies in these four different out of home settings consistently showed that adapting portion sizes of meat and vegetables was effective to reduce meat consumption and increase vegetable consumption, while maintaining high guest satisfaction. Guest satisfaction even increased when vegetables were presented and prepared in a more attractive and tasty way. Thus, adapting portion sizes of meat and vegetables provides a viable strategy to stimulate healthy and environmentally sustainable consumption patterns in out of home settings.

    Chemical and Enzymatic Modification of Chitosan to Produce New Functional Materials with Improved Properties
    Boeriu, Carmen G. ; Broek, Lambertus A.M. van den - \ 2019
    In: Chitin and Chitosan / van den Broek, Lambertus A.M., Boeriu, Carmen G., Wiley - ISBN 9781119450436 - p. 245 - 258.
    Chitin and chitosan are natural biopolymers with applications in different areas. However, to broaden its application area, research is needed to impart new functionality, develop new and better materials and to explore the whole potential of chitin/chitosan derivatives. These derivatives can be made by chemical and enzymatic synthesis. Here, the main classes of chitosan derivatives obtained by using green and sustainable conversion routes and as much as possible using green and/or bio‐based chemicals for modification and their potential areas for application are discussed.
    Chitin and Chitosan as Sources of Bio‐Based Building Blocks and Chemicals
    Kaisler, Malgorzata ; Broek, Lambertus A.M. van den; Boeriu, Carmen G. - \ 2019
    In: Chitin and Chitosan / van den Broek, Lambertus A.M., Boeriu, Carmen G., Wiley - ISBN 9781119450436 - p. 229 - 244.
    Chitin and chitosan polymers are a valuable source of functional chemicals and materials. Chemical and/or enzymatic depolymerisation processes have been developed for the production of chitooligosaccharides (COS), N‐acetylglucosamine (GlcNAc) and glucosamine (GlcN), which have a wide variety of applications. New technologies are now emerging to convert chitin and its derivatives into platform chemicals. Chemical liquefaction of chitin can lead to bulk chemicals like acetic acid and platform chemicals like hydroxymethylfurfural (HMF) and amine‐containing monomers for polymers, in low yield. The monomers GlcNAc and GlcN can be converted into N‐containing HMF derivatives, opening a pathway for furan‐based monomers for polyamides. Selective catalytic oxidation of GlcN results in the production of D‐glucosaminic acid (DGA). This acid is a valuable building block for the synthesis of various amino acids for biomedical applications and bio‐based chiral polyamides. Further technological improvements are necessary to increase the selectivity and efficiency of reactions, particularly for the conversion of polymeric chitin and chitosan into building blocks.
    Chitin and Chitosan : Properties and Applications
    Broek, Lambertus A.M. van den; Boeriu, Carmen G. - \ 2019
    John Wiley and Sons - ISBN 9781119450436 - 510 p.
    Offers a comprehensive guide to the isolation, properties and applications of chitin and chitosan

    Chitin and Chitosan: Properties and Applications presents a comprehensive review of the isolation, properties and applications of chitin and chitosan. These promising biomaterials have the potential to be broadly applied and there is a growing market for these biopolymers in areas such as medical and pharmaceutical, packaging, agricultural, textile, cosmetics, nanoparticles and more.

    The authors – noted experts in the field – explore the isolation, characterization and the physical and chemical properties of chitin and chitosan. They also examine their properties such as hydrogels, immunomodulation and biotechnology, antimicrobial activity and chemical enzymatic modifications. The book offers an analysis of the myriad medical and pharmaceutical applications as well as a review of applications in other areas. In addition, the authors discuss regulations, markets and perspectives for the use of chitin and chitosan. This important book:

    Offers a thorough review of the isolation, properties and applications of chitin and chitosan.
    Contains information on the wide-ranging applications and growing market demand for chitin and chitosan
    Includes a discussion of current regulations and the outlook for the future

    Written for Researchers in academia and industry who are working in the fields of chitin and chitosan, Chitin and Chitosan: Properties and Applications offers a review of these promising biomaterials that have great potential due to their material properties and biological functionalities.
    Beet pulp as an alternative to chemicals in dishwasher detergents and the leather industry
    Broek, Ben van den; Haveren, Jacco van - \ 2019
    The new tanning solutions come from sugar beet pulps
    Broek, Ben van den - \ 2019
    Nieuwe toepassingen voor bietenpulp in vaatwasmiddel en leerindustrie
    Broek, Ben van den; Haveren, Jacco van - \ 2019
    Het potentieel van garnalenpel als hernieuwbare grondstof
    Broek, Ben van den - \ 2019

    Wat hebben garnalen, kakkerlakken en schimmels dat wij niet hebben? Chitine, een van de meest voorkomende biologische materialen op aarde. Een veelzijdig materiaal ook, want toekomstige toepassingen variëren van een alternatief voor pesticiden tot een ijzersterk bouwmateriaal.

    Blue Bioeconomy Forum : Roadmap for the blue bioeconomy
    Ligtvoet, A. ; Maier, F. ; Sijtsma, L. ; Broek, L.A.M. van den; Doranova, A. ; Eaton, D. ; Guznajeva, T. ; Kals, J. ; Gallou, M. Le; Poelman, M. ; Saes, L. ; Zhechkov, R. - \ 2019
    Brussels : European Commission - ISBN 9789292027377 - 63 p.
    Blue Bioeconomy Forum : Highlights: Summary of the roadmap and a selection of viable and innovative projects
    Ligtvoet, A. ; Maier, F. ; Sijtsma, L. ; Broek, L.A.M. van den; Safi, Carl ; Doranova, A. ; Eaton, D. ; Guznajeva, T. ; Kals, J. ; Gallou, M. Le; Poelman, M. ; Saes, L. ; Zhechkov, R. - \ 2019
    Brussels : European Commission (Blue Bioeconomy Forum Newsletter ) - ISBN 9789292027308 - 42 p.
    Effect of challenge dose of plasmid-mediated extended-spectrum β-lactamase and AmpC β-lactamase producing Escherichia coli on time-until-colonization and level of excretion in young broilers
    Dame-Korevaar, Anita ; Fischer, Egil A.J. ; Goot, Jeanet van der; Velkers, Francisca ; Broek, Jan van den; Veldman, Kees ; Ceccarelli, Daniela ; Mevius, Dik ; Stegeman, Arjan - \ 2019
    Veterinary Microbiology 239 (2019). - ISSN 0378-1135
    Animal model - Antibiotic resistance - Dose-response - Inoculation - Poultry - Transmission

    Plasmid-mediated extended-spectrum β-lactamase and AmpC β-lactamase (ESBL/pAmpC) producing bacteria are present at all levels of the broiler production pyramid. Young birds can be found positive for ESBL/pAmpC-producing Escherichia coli shortly after arrival at farm. The aim of this study was to determine the effect of different challenge doses of ESBL/pAmpC-producing E. coli on time-until-colonization and the level of excretion in young broilers. One-day-old broilers (specific-pathogen free (SPF) and conventional Ross 308) were housed in isolators and challenged with 0.5 ml ESBL/pAmpC-producing E. coli strains of varying doses (101–105 CFU/ml). Presence and concentration (CFU/gram feces) of ESBL/pAmpC-producing E. coli and total E. coli were determined longitudinally from cloacal swabs, and in cecal content 72 h after challenge. Higher challenge doses resulted in shorter time-until-colonization. However, even the lowest dose (101 CFU/ml) resulted in colonization of the broilers which excreted >106 CFU/gram feces 72 h after inoculation. Conventional broilers were colonized later than SPF broilers, although within 72 h after challenge all broilers were excreting ESBL/pAmpC-producing E. coli. A probabilistic model was used to estimate the probability of colonization by initial inoculation or transmission. The higher the dose the higher the probability of excreting ESBL/pAmpC-producing E. coli as a result of inoculation. In conclusion, low initial doses of ESBL/pAmpC-producing E. coli can result in rapid colonization of a flock. Interventions should thus be aimed to eliminate ESBL/pAmpC-producing bacteria in the environment of the hatchlings and measures focusing at reducing colonization and transmission of ESBL/pAmpC-producing E. coli should be applied shortly after hatching.

    Korenwolfproject veelbelovend, maar toch gaat het slecht
    Müskens, Gerhard ; Haye, Maurice la; Kats, Ruud van; Kuiters, Loek - \ 2019
    Assessing the climate regulation potential of Agricultural soils using a decision support tool adapted to stakeholders' needs and possibilities
    Broek, Marijn Van de; Henriksen, Christian Bugge ; Ghaley, Bhim Bahadur ; Lugato, Emanuele ; Kuzmanovski, Vladimir ; Trajanov, Aneta ; Debeljak, Marko ; Sandén, Taru ; Spiegel, Heide ; Decock, Charlotte ; Creamer, Rachel ; Six, Johan - \ 2019
    Frontiers in Environmental Science 7 (2019). - ISSN 2296-665X
    Soils perform many functions that are vital to societies, among which their capability to regulate global climate has received much attention over the past decades. An assessment of the extent to which soils perform a specific function is not only important to appropriately value their current capacity, but also to make well-informed decisions about how and where to change soil management to align the delivered soil functions with societal demands. To obtain an overview of the capacity of soils to perform different functions, accurate and easy-to-use models are necessary. A problem with most currently-available models is that data requirements often exceed data availability, while generally a high level of expert knowledge is necessary to apply these models. Therefore, we developed a qualitative model to assess how agricultural soils function with respect to climate regulation. The model is driven by inputs about agricultural management practices, soil properties and environmental conditions. To reduce data requirements on stakeholders, the 17 input variables are classified into either (1) three classes: low, medium and high or (2) the presence or absence of a management practice. These inputs are combined using a decision tree with internal integration rules to obtain an estimate of the magnitude of N2O emissions and carbon sequestration. These two variables are subsequently combined into an estimate of the capacity of a soil to perform the climate regulation function. The model was tested using data from long-term field experiments across Europe. This showed that the model is generally able to adequately assess this soil function across a range of environments under different management practices. In a next step, this model will be combined with models to assess other soil functions (soil biodiversity, primary productivity, nutrient cycling and water regulation and purification). This will allow the assessment of trade-offs between these soil functions for agricultural land across Europe.
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