Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Size-exclusion simulated moving bed chromatographic protein refolding
Freydell, E. ; Bulsink, Y. ; Hateren, S. van; Wielen, L. van der; Eppink, M.H.M. ; Ottens, M. - \ 2010
Chemical Engineering Science 65 (2010)16. - ISSN 0009-2509 - p. 4701 - 4713.
hydrophobic interaction chromatography - ion-exchange chromatography - escherichia-coli - human proinsulin - oxidative renaturation - inclusion-bodies - lysozyme - aggregation - purification - polymerization
Size-exclusion chromatographic refolding (SECR) has successfully proven its capability to refold a variety of proteins using a range of gel filtration column materials. Several approaches have also been undertaken to improve the refolding yield of these systems, mostly under batch operation. Although, these approaches may lead to an increase on refolding yield, it is not expected that they will lead to significant increases in other important process indicators, such as volumetric productivity, specific eluent consumption and product concentration in the product stream; as these indicators are strongly dependent on the mode of operation. To overcome the shortcomings of batch chromatography, the size-exclusion refolding reactor may be operated in a continuous mode, with the aid of Simulated Moving Bed (SMB) technology. Albeit, SMB technology has inherent advantages over batch chromatography, these have been proven mainly in the context of conventional purifications and are still to be addressed in the context of chromatographic refolding reactors. In this work we report the on-column refolding of an industrially relevant protein, produced in inclusion bodies, by batch size-exclusion chromatography (SEC) and simulated moving bed size-exclusion chromatography (SMBSEC). The presented study encompasses: (1) a statistical design of experiments (DOE) to study the combined effect of the mobile phase pH (9.0–11.20) and the feed concentration of denatured and reduced protein (Cf,D&R=2.50–7.50 mg ml-1) on the refolding yield of the model protein; (2) a mechanistic analysis of the SMBSECR data, using a detailed model that accounts for both separation and refolding; and (3) a detailed comparison of the SMBSECR against the SECR, based both on quantitative and qualitative criteria. Our work showed that: (1) refolding yields of 50% are attainable by tuning pH and Cf,D&R, and that the positive effect of pH is strongly dependent on the Cf,D&R; (2) the modeling tool captured well the SMBSECR behavior, based solely on the effect that Cf,D&R has on the reaction rates; and (3) the volumetric productivity of the SMBSECR is about 53 times higher than that of the SECR, the specific solvent consumption is approximately 1/10th of that of the SECR, and the concentration of the product (i.e., native protein), leaving the SMBSECR, is roughly 4.5-fold higher than the one leaving the SECR. Accordingly, the comparison revealed the significant advantages that SMB technology has to offer to the design of chromatographic refolding reactors
Compounds repellent to Delia radicum (L.) (Dipt., Anthomyiidae)
Ouden, H. Den; Bulsink, A. ; Theunissen, J. - \ 1996
Journal of Applied Entomology 120 (1996). - ISSN 0931-2048 - p. 427 - 432.
Redox chemistry of biological tungsten: an EPR study of the aldehyde oxidoreductase from Pyrococcus furiosus.
Arendsen, A.F. ; Vocht, M. de; Bulsink, Y.B.M. ; Hagen, W.R. - \ 1996
Journal of Biological Inorganic Chemistry 1 (1996). - ISSN 0949-8257 - p. 292 - 296.
Crystallization and preliminary crystallographic analysis of the putative [6Fe-6S] prismane protein from Desulfovibrio vulgaris (Hildenborough)
Arendsen, A.F. ; Bulsink, Y. ; Hagen, W.R. ; Hadden, J. ; Card, G. ; McAlphine, A. ; Zaitsev, V. ; Lindley, P. - \ 1996
Acta Crystallographica Section D-Biological Crystallography D52 (1996). - ISSN 0907-4449 - p. 1211 - 1213.
Resonance Raman evidence for non-heme Fe-O species in the [6Fe-6S]-containing iron-sulfur proteins from sulfate-reducing bacteria.
Vocht, M.L. de; Kooter, I.M. ; Bulsink, Y.B. ; Hagen, W.R. ; Johnson, M.K. - \ 1996
Journal of the American Chemical Society 118 (1996). - ISSN 0002-7863 - p. 2766 - 2767.
Influence of charge and polarity on the redox potentials of high-potential iron-sulfur proteins: evidence for the existence of two groups.
Heering, H.A. ; Bulsink, Y.B.M. ; Hagen, W.R. ; Meyer, T.E. - \ 1995
Biochemistry 34 (1995). - ISSN 0006-2960 - p. 14675 - 14686.
Reversible super-reduction of the cubane [4Fe-4S](3+;2+;1+) in the high-potential iron-sulfur protein under non-denaturing conditions: EPR spectroscopic and electrochemical studies.
Heering, H.A. ; Bulsink, Y.B.M. ; Hagen, W.R. ; Meyer, T.E. - \ 1995
European Journal of Biochemistry 232 (1995). - ISSN 0014-2956 - p. 811 - 817.
Kwaliteitsbeheersing in de bloembollenteelt : ziekzoeken in het veld en uitzoeken in de schuur
Saedt, A.P.H. ; Bulsink, A.J. ; Berg, J.V. van den - \ 1982
Wageningen : IMAG (Rapport / Instituut voor Mechanisatie en Gebouwen no. 39) - 50
destructie - bloembollen - prestatieniveau - plantenziekten - gewasbescherming - kwaliteit - vectorbestrijding - vectoren - destruction - ornamental bulbs - performance - plant diseases - plant protection - quality - vector control - vectors
Allerlei bestaande en enkele nieuwe selektiemethoden, waaronder de toepassing van een speciaal ontwikkelde ziekzoekwagen, worden onderling vergeleken
Taaktijden voor het broeien van lelies
Bosch, D. ; Bulsink, A.J. - \ 1978
Wageningen : Instituut voor Mechanisatie, Arbeid en Gebouwen (Rapport / I.M.A.G. no. 5) - 88
liliaceae - bloembollen - agrarische bedrijfsvoering - agrarische bedrijfsplanning - forceren van planten - nederland - ornamental bulbs - farm management - farm planning - forcing - netherlands
De zeer snelle uitbreiding van de lelieteelt en de ingrijpende wijzigingen in de teeltmethoden waren aanleiding tot dit onderzoek, dat beoogde het tekort aan taaktijden op te vullen. Dit rapport wil een bijdrage te leveren tot meer zicht op de arbeidsbesteding bij deze teelt.
Arbeidsbehoefte- en organisatie in de bolbloementrekkerij
Bulsink, A.J. - \ 1973
Wageningen : Instituut voor Tuinbouwtechniek (Intern verslag / Instituut voor Tuinbouwtechniek 73) - 38
bloembollen - oogsten - arbeidsvereisten - forceren van planten - tulpen - sierteelt - snijbloemen - ornamental bulbs - harvesting - labour requirements - forcing - tulips - ornamental horticulture - cut flowers
Na overleg met het Laboratorium voo r Bloembollenonderzoek en het Consulentschap voor de Bloembollenteelt te Lisse is medio november 1971 begonnen met het arbeidskundig onderzoek in de bolbloementrekkerij. In eerste instantie werd besloten alleen de tulp in dit onderzoek te betrekken. De volgende broeimethoden zijn bestudeerd: 1. Broei in kistjes op tabletten in trekkassen; 2. Broei in kistjes op pallets in schuurkas; 3. Vollegrondsbroei in rolkas en warenhuis. Het onderzoek werd uitgevoerd op 2 bedrijven in de bloembollenstreek en op 2 bedrijven in het oude "broei"centrum Rijnsburg.
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