Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Effect of sterilization and of dietary fat and carbohydrate content on food intake, activity level, and blood satiety–related hormones in female dogs
Schauf, S. ; Salas-Mani, A. ; Torre, C. ; Bosch, G. ; Swarts, H. ; Castrillo, C. - \ 2016
Journal of Animal Science 94 (2016)10. - ISSN 0021-8812 - p. 4239 - 4250.
Activity - Dietary fat and carbohydrates - Dog sterilization - Food intake - Satiety hormones

Animal sterilization is suggested to promote food overconsumption, although it is unknown whether this effect is mediated by variations in satiety-related hormones, which are released in response to food intake. The aim of this study was to evaluate the effect of sterilization and of the main energy-delivery nutrients, fat and nonstructural carbohydrates, on food intake, blood concentration of satiety-related hormones, and activity level in dogs. In a 2-phase experiment (phase I [Ph.I], 74 d, and Ph.II, 84 d), 12 female Beagle dogs were assigned to a control group (intact in both phases) and a sterilization group (spayed 20 d before Ph.II). In each phase, dogs received a high-carbohydrate (HC) diet (313 and 105 g/kg DM starch and fat, respectively) and a high-fat (HF) diet (191 and 213 g/kg DM starch and fat, respectively), both high in total dietary fiber (>200 g/kg DM) and providing 27% ME as protein, in 2 consecutive periods following a crossover arrangement. During each period, dogs’ voluntary DMI and activity level were recorded during 5 d. Then, energy allowance was restricted to 0.7 maintenance and the level of intake of a common challenge food offered 4 h after feeding the experimental diets (challenge food intake [ChFI]) was used as an index of the satiety state of dogs. Blood concentration of active ghrelin, cholecystokinin (CCK), total peptide YY (PYY), and insulin were determined before and 15, 60, 120, 240, and 360 min after feeding. Voluntary DMI was greater (P <0.05) in HF-fed dogs, but ChFI did not differ between diets (P > 0.10). Dogs fed the HF diet showed a lower increase of CCK at 120 (P <0.01) and 240 min (P <0.05), resulting in a lower (P <0.001) total area under the curve from 0 to 240 min (tAUC0–240). A lower PYY elevation (P <0.05) was also found in HF-fed dogs at 120 min. Only active ghrelin concentration at 240 min and insulin tAUC0–240 correlated (P <0.05) with ChFI (r = 0.357 and r = −0.364, respectively), suggesting a role of these hormones in appetite. Dog sterilization did not affect voluntary DMI, ChFI, or blood hormones (P > 0.10) but led to a reduced activity level compared with control dogs (P <0.05). In summary, dog sterilization was not associated with an impaired appetite control. Feeding dogs the HF diet led to energy overconsumption and to a lower blood elevation of CCK and PYY but was not associated with a weaker satiating effect 4 h later compared with the HC diet.

Postprandial concentration of satiety-related hormones and moetabolites in Beagle dogs after consumption of a high carbohydrate or a high fat diet
Schauf, S. ; Salas, A. ; Torre, C. ; Bosch, G. ; Swarts, J.J.M. ; Rehfeld, J.F. ; Castrillo, C. - \ 2012
In: Congress proceedings 16th Congress of the European Society of Veterinary and Comparative Nutrition, Bydgoszcz, Poland,13-15 September 201213. - Bydgoszcz, Poland : Multikop Sp. Z o.o. - ISBN 9788392173229 - p. 21 - 21.
Fermentation characteristics of various animal tissues by cheetah faecal inoculum
Depauw, S. ; Bosch, G. ; Becker, A. ; Hesta, M. ; Whitehouse-Tedd, K. ; Hendriks, W.H. ; Janssens, G.P.J. - \ 2011
In: Proceedings 15th congress European Society of Veterinary and Comparative Nutrition, Zaragoza, Spain, 14 - 16 September, 2011. - Zaragoza : Universidad Zaragoza - p. 46 - 46.
Introduction: Recent studies in the cheetah (Acinonyx jubatus) show a role for undigested animal tissues (e.g. bone, cartilage, hair, skin, feathers) in hindgut fermentation of this strict carnivore (Depauw et al, 2010 a, b). This study aimed to compare the in vitro fermentation kinetics and end-product profiles of different animal-derived substrates, using cheetah faeces as an inoculum. Materials and methods: Fresh faecal samples of eight captive cheetahs were collected within 15 min of defecation, pooled and processed to be used as inoculum. The following raw and homogenised (1mm sieve) substrates were used: rabbit bone, rabbit hair, rabbit skin, whole rabbit, chicken cartilage, beef, beef + bone, beef + cartilage, beef + hair, and beef + skin. Cumulative gas production was continuously recorded over a 72 h period and samples of fermentation liquids were taken at 2, 7, 24 and 72 hours of incubation to determine the end product profile (short-chain fatty acids (SCFA), branched-chain fatty acids (BCFA), ammonia, indole, phenol, p-cresol). Results and discussion: All animal substrates showed an early occurrence of maximum gas production rates (Tmax = 0.1-0.7 h), which confirms our previous findings. Cartilage was highly fermentable and showed the highest gas production as well as SCFA production. Beef exhibited a lower gas production rate than cartilage, and was slower to achieve high SCFA concentrations, which were only detected after 7 hours of incubation, compared to 2 hours for cartilage. Compared to cartilage and beef, whole rabbit, rabbit bone, and skin were low fermentable, and hair exhibited the lowest gas and SCFA production. Fermentation of bone yielded ammonia concentrations that were twice as high as all other substrates. The combination of a fermentable substrate (beef) with low fermentable animal tissue showed no clear interactions. Conclusion: The present data indicate that cartilage and beef are well fermentable substrates for the cheetah, with cartilage being the most fermentable of both. Low fermentable substrates (hair, skin, bone) in homogenised forms did not appear to reduce the fermentation of beef in this in vitro set up. References: available on request.
High-cell-density cultivation of yeasts on disaccharides in oxygen-limited batch cultures
Castrillo, J.I.C. ; Kaliterna, J. ; Weusthuis, R.A. ; Dijken, J.P. van; Pronk, J.T. - \ 1996
Biotechnology and Bioengineering 49 (1996). - ISSN 0006-3592 - p. 621 - 628.
Coordination of sucrose uptake and respiration in the yeast Debaryomyces yamadae
Kaliterna, J. ; Weusthuis, R.A. ; Castrillo, J.I. ; Dijken, J.P. van; Pronk, J.T. - \ 1995
Microbiology 141 (1995)7. - ISSN 1350-0872 - p. 1567 - 1574.
Screening in batch cultures identified Debaryomyces yamadae as a yeast that exhibits the Kluyver effect for sucrose: this disaccharide can be respired but, even under oxygen-limited conditions, alcoholic fermentation of sucrose does not occur. Ethanol, glycerol and arabitol were the main fermentation products during oxygen-limited growth on glucose in chemostat cultures. None of these fermentation products were produced in oxygen-limited chemostat cultures grown on sucrose and the fraction of the sucrose that could not be respired remained unused in the culture medium. This absence of alcoholic fermentation was not due to repression of the key fermentative enzymes pyruvate decarboxylase and alcohol dehydrogenase. In contrast to some other yeasts that exhibit a Kluyver effect, D. yamadae did not exhibit a preference for ethanol in batch cultures grown on mixtures of ethanol and sucrose. Sucrose metabolism in D. yamadae involves intracellular hydrolysis by an α-glucosidase. Incubation of weakly buffered cell suspensions with sucrose led to a rapid transient alkalinization, indicating the presence of a sucrose-proton symport system. The apparent substrate saturation constant of the sucrose-uptake system was 0.2 mmol l-1. Sucrose-dependent alkalinization rates were much lower in samples from oxygen-limited cultures than in samples from aerobic cultures. Transient responses of D. yamadae to oxygen limitation were investigated by applying a sudden decrease in the oxygen feed to aerobic sugar-limited chemostat cultures. In glucose grown cultures, this led to alcoholic fermentation and no significant accumulation of sugar occurred after the switch. In sucrose-limited cultures, sugar accumulation occurred instantaneously after the switch, and ethanol formation was virtually absent. The results indicate that the Kluyver effect for sucrose in D. yamadae, i.e. the adjustment of the glycolytic flux to the cells' respiratory capacity, is effected by rapid down-regulation of the capacity of the sucrose carrier under oxygen-limited conditions.
Transient responses of Candida utilis to oxygen-limitation Regulation of the Kluyver effect for maltose
Kaliterna, J. ; Weusthuis, R.A. ; Castrillo, J.I. ; Dijken, J.P. van; Pronk, J.T. - \ 1995
Yeast 11 (1995)4. - ISSN 0749-503X - p. 317 - 325.
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