Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Simulating streamflow variability and aquatic states in temporary streams using a coupled groundwater-surface water model
Querner, E.P. ; Froebrich, J. ; Gallart, F. ; Cazemier, M.M. ; Tzoraki, O. - \ 2016
Hydrological Sciences Journal 61 (2016)1. - ISSN 0262-6667 - p. 146 - 161.

In order to apply the EU Water Framework Directive for temporary streams, it is important to quantify the space–time development of different aquatic states. We report on research on the development of aquatic states for temporary streams in the Evrotas basin, Greece. The SIMGRO regional hydrological model was used in a GIS framework to generate flow time series for the Evrotas River and all major tributaries. Five flow phases were distinguished: flood conditions, riffles, connected pools, isolated pools and dry bed conditions. Thresholds based on local hydraulic characteristics were identified per stream reach and flow phase, enabling the frequency of flow phases per month and the average frequencies for all streams to be derived. Three historical scenarios within the 20th century, marking periods of major changes in water management, were investigated. Additionally, a climate scenario for the 2050s was analysed. Simulations revealed that low flows are now much lower, mainly because more groundwater is abstracted for irrigation. The consequence is that stretches of the river fall dry during several months, causing the ecological status to deteriorate.

Relationship between redox potential and the emergence of three submerged macrophytes
Zuidam, B.G. van; Cazemier, M. ; Geest, G.J. van; Roijackers, R.M.M. ; Peeters, E.T.H.M. - \ 2014
Aquatic Botany 113 (2014). - ISSN 0304-3770 - p. 56 - 62.
seed banks - sediment load - zannichellia-palustris - hydrogen-sulfide - lake-sediments - western-europe - shallow lake - germination - burial - impact
Sedimentation may have large negative effects on aquatic vegetation as burial of propagules can reduce emergence. Burial changes the redox potential around the propagules and this might be the mechanism that causes the observed burial effects. We conducted a laboratory experiment to evaluate the effect of redox potential on the emergence of three aquatic macrophytes from their propagules. Different redox potential treatments were applied by burying propagules of Potamogeton pusillus and Chara cf. contraria at three different depths and with two different sediments (loamy mud and sand). Propagules of Zannichellia palustris were also buried at three depths, but only with sand. Emergence of P. pusillus and Z. palustris decreased with increasing burial depth, while burial up to 5 cm depth had almost no effect on Chara cf. contraria. Burial with sand reduced emergence of P. pusillus more than burial with loamy mud, while composition of the burial sediment did not affect Chara cf. contraria. The redox potential treatments explained emergence of P. pusillus better than burial depth or composition of the burial sediment separately. There was a strong relationship between mean emergence of P. pusillus per treatment and redox potential of the treatment. Burial caused high mortality of the non-emerged propagules of P. pusillus and Z. palustris within a relatively short period of time. Our results show that redox potential could be an important factor in causing the effect of burial on emergence. On longer time scale, sedimentation has species-specific consequences potentially leading to changes in vegetation species composition.
Hydrological analysis of the Evrotas basin, Greece - Low flow characterization and scenario analisis
Cazemier, M. ; Querner, E.P. ; Lanen, H.A.J. van; Gallart, F. ; Prat, N. ; Tzoraki, O. ; Froebrich, J. - \ 2011
Wageningen : Alterra (Alterra-rapport 2249) - 92
hydrologie - waterlopen - aquatische ecologie - griekenland - stroming - seizoengebondenheid - hydrology - streams - aquatic ecology - greece - flow - seasonality
Biosensor immunoassay for flumequine in broiler serum and muscle
Haasnoot, W. ; Gercek, H. ; Cazemier, G. ; Nielen, M.W.F. - \ 2007
Analytica Chimica Acta 586 (2007)1-2. - ISSN 0003-2670 - p. 312 - 318.
linked-immunosorbent-assay - indirect competitive elisa - antibodies - residues - enrofloxacin - fluoroquinolones - ciprofloxacin - sarafloxacin - products - tissue
Flumequine (Flu) is one of the fluoroquinolones most frequently applied for the treatment of broilers in The Netherlands. For the detection of residues of Flu in blood serum of broilers, a biosensor immunoassay (BIA) was developed which was fast (7.5 min per sample) and specific (no cross-reactivity with other (fluoro)quinolones). This inhibition assay was based on a rabbit polyclonal anti-Flu serum and a CM5 biosensor chip coated with Flu which could be detected in the range of 15-800 ng mL(-1). For the detection of Flu in muscle, an easy extraction procedure in buffer was selected and the measuring range was from 24 to 4000 ng g(-1). Average recoveries of 66 till 75% were found with muscle samples spiked at 0.5, 1 and 2 times the maximum residue limit (MRL in muscle = 400 ng g(-1)) and the decision limit (CC alpha) and the detection capability (CC beta) were determined as 500 and 600 ng respectively. Incurred muscle samples were analysed by the BIA and by LC-MS/MS and a good correlation was found (R-2 = 0.998). Serum and muscle samples from with Flu treated broilers were analysed and the concentrations found in serum were always higher than those found in muscle (average serum/muscle ratio was 3.5) and this proved the applicability of the BIA in serum as predictor of the Flu concentration in muscle. (c) 2006 Elsevier B.V. All rights reserved.
BLUP begrijpen
Ducro, B.J. ; Cazemier, G. - \ 2006
IJslandse paarden 2006 (2006)april. - ISSN 1384-4334 - p. 38 - 41.
Biosensor detection of sulfonamides : from specific multi-sulfonamide assays
Haasnoot, W. ; Ploum, M.E. ; Korpimaeki, T. ; Cazemier, G. ; Pre, J.G. du; Kohen, F. - \ 2005
In: Rapid methods for biological and chemical contaminants in food and feed / van Amerongen, A., Barug, D., Wageningen : Wageningen Academic Publishers - ISBN 9076998531 - p. 321 - 337.
Promicromonospora pachnodae sp nov., a member of the (hemi)cellulolytic hindgut flora of larvae of the scarab beetle Pachnoda marginata
Cazemier, A.E. ; Verdoes, J.C. ; Reubsaet, F.A.G. ; Hackstein, J.H.P. ; Drift, C. van der; Camp, H.J.M. op den - \ 2003
Antonie van Leeuwenhoek: : Nederlandsch tijdschrift voor hygiëne, microbiologie en serologie 83 (2003)2. - ISSN 0003-6072 - p. 135 - 148.
termite guts - cellulomonas - degradation - arthropods - digestion - bacteria - gene - microorganisms - lignocellulose - purification
Intestinal microorganisms play an important role in plant fiber degradation by larvae of the rose chafer Pachnoda marginata. In the hindgut of the larvae 2.5 to 7.4 × 108 bacteria per ml of gut content with xylanase or endoglucanase activity were found. Bacteria in the midgut were not (hemi)cellulolytic, but the alkaline environment in this part of the intestinal tract functions as a precellulolytic phase, solubilizing part of the lignocellulosic material. Accordingly, the degradation of lignocellulose-rich material in Pachnoda marginata larvae appeared to be a combination of a physico-chemical and microbiological process. A number of different facultative anaerobic and strictly anaerobic bacteria with (hemi)cellulolytic activity were isolated from the hindgut. A dominant (hemi)cellulolytic species was a Gram positive, irregular shaped, facultative aerobic bacterium. Further physiological identification placed the isolate in the genus Promicromonospora. Comparative 16S rDNA analysis and phenotypic features revealed that the isolate represented a new species for which the name Promicromonospora pachnodae is proposed. P. pachnodae produced xylanases and endoglucanases on several plant derived polymers, both under aerobic and anaerobic conditions.
Single biosensor immunoassay for the detection of five aminoglycosides in reconstituted skimmed milk
Haasnoot, W. ; Cazemier, G. ; Koets, M. ; Amerongen, A. van - \ 2003
Analytica Chimica Acta 488 (2003)1. - ISSN 0003-2670 - p. 53 - 60.
performance liquid-chromatography - (dihydro)streptomycin residues - optical immunobiosensor - antibiotic residues - raw-milk - streptomycin - gentamicin - assay
The application of an optical biosensor (Biacore 3000), with four flow channels (Fcs), in combination with a mixture of four specific antibodies resulted in a competitive inhibition biosensor immunoassay (BIA) for the simultaneous detection of the five relevant aminoglycosides in reconstituted skimmed milk. Four aminoglycosides (gentamicin, neomycine, kanamycin and a streptomycin derivative) were immobilised onto the sensor surface of a biosensor chip (CM5) in the four Fcs of the biosensor system by amine coupling. In the Biacore, milk (reconstituted from skimmed milk powder) was 10 times diluted with a mixture of the four specific antibodies and injected through the four serially connected Fcs (1 min at a flow rate of 20 ¿l min¿1). The responses measured just prior to the injection (20 ¿l at a flow rate of 20 ¿l min¿1) of the regeneration solution (0.2 M NaOH + 20% acetonitril) were indicative for the presence or absence of the aminoglycosides in reconstituted milk. The limits of detection were between 15 and 60 ng ml¿1, which was far below the maximum residue limits (MRLs) (varying from 100 to 500 ng ml¿1) and the total run time between samples was 7 min.
Direct versus competitive biosensor immunoassays for the detection of (dihydro)streptomycin residues in milk
Haasnoot, W. ; Loomans, E.E.M.G. ; Cazemier, G. ; Dietrich, R. ; Verheijen, R. ; Bergwerff, A.A. ; Stephany, R.W. - \ 2002
Food and Agricultural Immunology 14 (2002). - ISSN 0954-0105 - p. 15 - 27.
surface plasmon resonance - dihydrostreptomycin - streptomycin - biosensor - immunoassy - milk
Immunoaffinity chromatography for the sample pretreatment of Taxus plant and cell extracts prior to analysis of taxanes by high-performance liquid chromatography
Theodoridis, G. ; Haasnoot, W. ; Cazemier, G. ; Schilt, R. ; Jaziri, M. ; Diallo, B. ; Papadoyannis, I.N. ; Jong, G.J. de - \ 2002
Journal of Chromatography. A, Including electrophoresis and other separation methods 948 (2002). - ISSN 0021-9673 - p. 177 - 185.
Taxus spp. - Immunoaffinity chromatography - Sample handling - Plant materials - Taxol - Paclitaxel - Deacetylbaccatin III - Taxanes
Direct biosensor immunoassays for the detection of nonmilk proteins in milk powder
Haasnoot, W. ; Olieman, K. ; Cazemier, G. ; Verheijen, R. - \ 2001
Journal of Agricultural and Food Chemistry 49 (2001)11. - ISSN 0021-8561 - p. 5201 - 5206.
plant proteins - soy - pea - soluble wheat proteins
The low prices of some nonmilk proteins make them attractive as potential adulterants in dairy products. An optical biosensor (BIACORE 3000) was used to develop a direct and combined biosensor immunoassay (BIA) for the simultaneous detection of soy, pea, and soluble wheat proteins in milk powders. Affinity-purified polyclonal antibodies raised against the three protein sources were immobilized in different flow channels (Fcs) on the biosensor chip (CM5). Dissolved milk powders were injected (20 L injections at 20 L min-1) through the serially connected Fcs, and the antibody-bound plant proteins were detected directly. The total run time between samples, including a regeneration step with 5 L of 10 mM HCl, was 5 min. The limits of detection in milk powder were below 0.1␘f plant protein in the total milk protein content. The antibodies also recognized some proteins from other plant sources, which made this BIA even more suitable as a broad screening assay for nonmilk proteins.
Application of generic monoclonal antibodies against sulfonamides in optical biosensors
Haasnoot, W. ; Kohen, F. ; Pre, J. du; Cazemier, G. ; Kemmers-Voncken, A. ; Bienenmann-Ploum, M. ; Verheijen, R. - \ 2000
In: Proceedings of the EuroResidue IV Conference, Veldhoven, The Netherlands
Sulphonamide Antibodies: From Specific Polyclonals to Generic Monoclonals
Haasnoot, W. ; Cazemier, G. ; Pre, J. du; Kemmers-Voncken, A. ; Bienenmann-Ploum, M. ; Verheijen, R. - \ 2000
Food and Agricultural Immunology 12 (2000). - ISSN 0954-0105 - p. 15 - 30.
Monoclonal antibodies against a sulfathiazole derivative for the immunochemical detection of sulfonamides
Haasnoot, W. ; Pre, J. Du; Cazemier, G. ; Kemmers-Voncken, A. ; Verheijen, R. ; Jansen, B.J.M. - \ 2000
Food and Agricultural Immunology 12 (2000). - ISSN 0954-0105 - p. 127 - 138.
To prepare monoclonal antibodies (mAbs) against the generic part of sulfonamides, a sulfathiazole derivative was chemically linked to carrier proteins in such a way that the aromatic amino group, common to all sulfonamides, was distal to the proteins. Four mice were immunized with the sulfathiazole-protein derivatives. The spleen cells of one of the mice were fused with myeloma cells to produce hybridomas of which the supernatants were screened in an indirect ELISA (iELISA) for the presence of sulfathiazole antibodies. After cloning, positive supernatants were tested in a competitive iELISA (ciELISA) for inhibition with 18 sulfonamides. This resulted in four different mAbs (all IgG1 kappa light chain) which recognized several sulfonamides. By use of the best monoclonal (27G3) and an optimized ciELISA protocol, eight structurally different sulfonamides showed 50% inhibition at concentrations less than 100 ngml-1 or 5 ng/well. However, other relevant sulfonamides (such as sulfadimidine, sulfatroxazole and sulfachloropyrazine) were detected at a high level only with this mAb. This means that the ciELISA (with the best Mab) showed a broad specificity for sulfonamides but the sensitivity towards the different sulfonamides varied too much to call it a generic sulfonamide ELISA.
Ueberwachung der Fischmigration in den Niederlanden
Cazemier, W.G. - \ 1999
Unknown Publisher
Ueberwachung der Fischmigration in den Niederlanden. Schlussbericht. Projekt "Rueckkehr der Langdistanz-Wanderfische in den Rhein" (Lachs 2000 Projekt) IV Massnahmen auf nierderlaendschem Hoheitsgebiet
Cazemier, W.G. - \ 1999
Unknown Publisher (BERICHT C012/99)
Immunochemical detection of aminoglycosides in milk and kidney
Haasnoot, W. ; Stouten, P. ; Cazemier, G. ; Lommen, A. ; Nouws, J.F.M. ; Keukens, H.J. - \ 1999
The Analyst 124 (1999)3. - ISSN 0003-2654 - p. 301 - 305.
In 1996, the European Union established provisional maximum residue limits (MRL) for gentamicin, neomycin, streptomycin and dihydrostreptomycin in milli and tissue (0.1-5 mg kg-1). For the detection of these four aminoglycosides, three enzyme linked immunosorbent assays (ELISA) for applications in milk and kidney were developed. The screening of defatted and diluted milk resulted in limits of determination (LDM) of < 0.01 mg l-1. Kidney samples were deproteinized with a trichloroacetic acid solution (3%) and after filtration and the addition of buffer, aliquots were used in the ELISA. The LDM of the four aminoglycosides in kidney were < 0.05 mg kg-1. The ELISA were found suitable for the semi-quantitative screening of milk and kidney for the presence of the four aminoglycosides far below the MRL levels. In randomly taken milk samples (n = 776) and in kidneys derived from healthy pigs (n = 124), the aminoglycoside residues found were far below their established MRL. In eight out of the 94 kidney samples obtained from diseased animals after emergency slaughter, aminoglycoside residues were above the MRL.
A ß-1,4-endoglucanase-encodiing gene from Cellulomonas pachnodae
Cazemier, A.E. ; Verdoes, J.C. ; Camp, H.J.M. op den; Hackstein, J.H.P. ; Ooyen, A.J.J. van - \ 1999
Applied Microbiology and Biotechnology 52 (1999). - ISSN 0175-7598 - p. 232 - 239.
De vismigratie via de bekkenvistrap bij de Maasstuw te Sambeek in 1997
Cazemier, W.G. ; Jong, P. de; Jong, H.B.H.J. de - \ 1998
Unknown Publisher (RAPPORT C018/98)
Onderzoek naar de salmonidenmigratie via de grote rivieren in 1996
Cazemier, W.G. ; Jong, H.B.H.J. de - \ 1998
Unknown Publisher (RAPPORT C016/98)
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