Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Application of the classic limulus test and the quantitative kinetic chromogenic LAL method for evaluation of endotoxin concentration in indoor air
    Gorny, R. ; Douwes, J. ; Versloot, P. ; Heederik, D. ; Dutkiewicz, J. - \ 1999
    Annals of Agricultural and Environmental Medicine 6 (1999). - ISSN 1232-1966 - p. 45 - 51.
    Blootstelling aan microbiële agentia bij GFT-composteerders
    Douwes, J. ; Dubbeld, H. ; Zwieten, L. van; Doekes, G. ; Wouters, I. ; Steerenberg, P. ; Heederik, D. - \ 1999
    Tijdschrift voor Toegepaste Arbowetenschap 3 (1999). - ISSN 1568-4733 - p. 18 - 24.
    Fungal extracellular polysaccharides in house dust as a marker for exposure to fungi : Relations with culturable fungi, reported home dampness and respiratory symptoms
    Douwes, J. ; Sluis, B. van der; Doekes, G. ; Leusden, F. van; Wijnands, L. ; Strien, R. van; Verhoeff, A. ; Brunekreef, B. - \ 1999
    Journal of Allergy and Clinical Immunology 103 (1999). - ISSN 0091-6749 - p. 494 - 500.
    Review of methods applicable to the assessment of mold exposure to children
    Dillon, H.K. ; Miller, J.D. ; Sorenson, W.G. ; Douwes, J. ; Jacobs, R.R. - \ 1999
    Environmental Health Perspectives 107 (1999). - ISSN 0091-6765 - p. S473 - S480.
    GFT-afval als bron van microbiele luchtverontreiniging: onderzoek naar microbiele belasting in woningen, onderzoek naar de blootstelling en eventuele gezondheidseffecten bij vuilnisophalers.
    Wouters, I.M. ; Douwes, J. ; Zock, J.P. ; Doekes, G. ; Heederik, D.J.J. - \ 1998
    Unknown Publisher
    Interlaboratory comparison of endotoxin assays in agricultural dusts: mass spectroscopy results.
    Reynolds, S.J. ; Larsson, L. ; Milton, D.K. ; Thorne, P.S. ; Donham, K.J. ; Croteau, E.A. ; Lewis, D. ; Whitmer, M. ; Heederik, D.J.J. ; Douwes, J. ; Connaughton, I. - \ 1998
    In: 4th International Symposium: Rural Health and Safety in a Changing World, Saskatoon, Canada - p. O80 - O80.
    Does indoor storage of compostable waste influence the concentration of microbial agents in house dust?
    Wouters, I.M. ; Zock, J.P. ; Douwes, J. ; Doekes, G. ; Heederik, D.J.J. ; Brunekreef, B. - \ 1998
    In: 3rd International Conference on Bioaerosols, Fungi and Mycotoxins, New York, USA - p. 85 - 85.
    Bio-aerosol exposure and upper airway inflammation assessed by nasal lavage in household waste collectors.
    Wouters, I.M. ; Hilhorst, S.K.M. ; Kleppe, P. ; Douwes, J. ; Doekes, G. ; Heederik, D.J.J. - \ 1998
    In: 3rd International Conference on Bioaerosols, Fungi and Mycotoxins, New York, USA - p. 60 - 60.
    Endotoxin and beta-(1,3)-D-Glucan in house dust in relation to peak flow variability in asthmatic children.
    Doekes, G. ; Douwes, J. ; Zuidhof, A. ; Zee, S. van der; Brunekreef, B. - \ 1998
    European Respiratory Journal 12 (1998). - ISSN 0903-1936 - p. 13S - 13S.
    Airway inflammation assessed by nasal lavage in household waste collectors.
    Wouters, I.M. ; Douwes, J. ; Doekes, G. ; Heederik, D.J.J. ; Steerenberg, P.A. - \ 1998
    European Respiratory Journal 12 (1998). - ISSN 0903-1936 - p. 32S - 32S.
    Acute lung function changes and low endotoxin exposures in the potato processing industry.
    Zock, J.P. ; Hollander, A. ; Heederik, D. ; Douwes, J. - \ 1998
    American Journal of Industrial Medicine 33 (1998). - ISSN 0271-3586 - p. 384 - 391.
    American Thoracic Society: respiratory health hazards in agriculture.
    Schenker, M.B. ; Christiani, D. ; Cormier, Y. ; Dimich-Ward, H. ; Doekes, G. ; Dosman, J. ; Douwes, J. ; Dowling, K. ; Enarson, D. ; Green, F. ; Heederik, D. ; Husman, K. - \ 1998
    American Journal of Respiratory and Critical Care Medicine 158 (1998). - ISSN 1073-449X - p. S1 - S76.
    Loss of the plasma membrane-bound protein gas1p in saccharomyces cerevisiae results in the release of beta1,3-glucan into the medium and induces a compensation mechanism to ensure cell wall integrity.
    Ram, A.F.J. ; Kapteyn, J.C. ; Montijn, R.C. ; Caro, L.H.P. ; Douwes, J.E. ; Baginsky, W. ; Mazur, P. ; Ende, H. van den; Klis, F.M. - \ 1998
    Journal of Bacteriology 180 (1998). - ISSN 0021-9193 - p. 1418 - 1424.
    In vitro effects of 3,3',4,4'-tetrachlorobiphenyl and its hydroxymetabolites on mitochondrial function.
    Lans, M.C. ; Kubiczak, G. ; Douwes, J. ; Brouwer, A. - \ 1998
    In: Organohalogen Compounds: toxicology, environment, food, exposure-risk / Hutzinger, O., Fielder, H., - p. 103 - 106.
    Longitudinal changes in inflammatory markers in nasal lavage of cotton workers; relation to endotoxin exposure and lung function changes.
    Keman, S. ; Jetten, M. ; Douwes, J. ; Borm, P.J.A. - \ 1998
    International Archives of Occupational and Environmental Health 71 (1998). - ISSN 0340-0131 - p. 131 - 137.
    Endotoxin and beta(1->3)-glucan in house dust and the relation with home characteristics: a pilot study in 25 German houses.
    Douwes, J. ; Doekes, G. ; Heinrich, J. ; Koch, A. ; Bischof, J. ; Brunekreef, B. - \ 1998
    Indoor Air 8 (1998). - ISSN 0905-6947 - p. 255 - 263.
    Respiratory health effects of indoor microbial exposure : a contribution to the development of exposure assessment methods
    Douwes, J. - \ 1998
    Agricultural University. Promotor(en): B. Brunekreef; R. Rylander; G. Doekes. - S.l. : Douwes - ISBN 9789054859352 - 171
    ademhalingssysteem - microbiële besmetting - luchtverontreiniging - respiratory system - microbial contamination - air pollution

    The studies described in this thesis validated an existing method to measure bacterial endotoxin and explored new methods for measuring mold components in the environment, particularlyβ(1→3)-glucans and extracellular polysaccharides (EPS). These assays were used to study levels of microbial components in the home and occupational environment, and to investigate their relationships with respiratory health in children or workers, respectively.

    It was shown that results of endotoxin measurements may differ considerably between laboratories when different sampling, extraction, and storage procedures are employed. Highest yields were found when samples were extracted with 0.05% Tween-20 in water. Repeated freezing and thawing resulted in a considerable endotoxin loss.β(1→3)-glucan, endotoxin and EPS from Aspergillus and Penicillium spp. (EPS- Asp/Pen ) were readily detectable in house dust samples. Endotoxin andβ(1→3)-glucan levels were, at least for living room floors, significantly associated with certain home characteristics, particularly heating system and/or age of the home. Bothβ(1→)-glucan and EPS- Asp/Pen were significantly but only weakly correlated with culturable fungi in house dust. Living room floor concentrations of EPS- Asp/Pen were positively associated with occupant-reported home dampness and respiratory symptoms in children, while no such associations were found for bedroom and mattress concentrations.

    In another study among children it was shown thatβ(1→3)-glucan in house dust from living room floors was strongly associated with peak flow (PEF) variability, particularly in atopic and/or asthmatic children. No associations were found forβ(1→3)-glucan levels on bedroom floors and mattresses. Unadjusted regression models showed that endotoxin levels were also significantly associated with PEF variability, while after adjusting for other known risk factors no significant association was found. A study among compost workers showed that microbial exposure induces acute and possibly chronic inflammation in the upper airways with a mechanism that suggests non-immune or possibly type III but not type I allergic inflammation.

    It is concluded that the studies reported in this thesis contribute to new methods for microbial exposure assessment that offer advantages for use in epidemiological studies compared to traditionally employed culture-based methods.

    Zock, P.L. - \ 1998
    In: Informatorium voor voeding en dietetiek / Geerts-van der Weij, A., van Binsbergen, J.J., Dommelen-van Wamelen, J.A., Douwes, A., Poen, H., de Vries, J.H.M., Houten : Bohn Stafleu van Loghem - p. 1 - 17.
    Rapport 'Maten, Gewichten en Codenummers 1997'.
    Heijden, L.J.M. van der - \ 1997
    In: Informatorium voor voeding en dietetiek / Geerts-van der Weij, A., van Binsbergen, J.J., Douwes, A., Poen, H., de Vries, J.H.M.,
    Nederlands Voedingsstoffenbestand: de NEVO-tabel.
    Heijden, L.J.M. van der - \ 1997
    In: Informatorium voor voeding en dietetiek / Geerts-van der Weij, A., van Binsbergen, J.J., Douwes, A., Poen, H., de Vries, J.H.M.,
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