Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Anaplasma phagocytophilum evolves in geographical and biotic niches of vertebrates and ticks
    Jaarsma, Ryanne I. ; Sprong, Hein ; Takumi, Katsuhisa ; Kazimirova, Maria ; Silaghi, Cornelia ; Mysterud, Atle ; Rudolf, Ivo ; Beck, Relja ; Földvári, Gábor ; Tomassone, Laura ; Groenevelt, Margit ; Everts, Reinard R. ; Rijks, Jolianne M. ; Ecke, Frauke ; Hörnfeldt, Birger ; Modrý, David ; Majerová, Karolina ; Votýpka, Jan ; Estrada-Peña, Agustín - \ 2019
    National Institute for Public Health and the Environment (RIVM)
    Anaplasma phagocytophilum - Ticks - Ixodidae - Molecular epidemiology - Transmission dynamics - Network analysis
    Background Anaplasma phagocytophilum is currently regarded as a single species. However, molecular studies indicate that it can be subdivided into ecotypes, each with distinct but overlapping transmission cycle. Here, we evaluate the interactions between and within clusters of haplotypes of the bacterium isolated from vertebrates and ticks, using phylogenetic and network-based methods. Methods The presence of A. phagocytophilum DNA was determined in ticks and vertebrate tissue samples. A fragment of the groEl gene was amplified and sequenced from qPCR-positive lysates. Additional groEl sequences from ticks and vertebrate reservoirs were obtained from GenBank and through literature searches, resulting in a dataset consisting of 1623 A. phagocytophilum field isolates. Phylogenetic analyses were used to infer clusters of haplotypes and to assess phylogenetic clustering of A. phagocytophilum in vertebrates or ticks. Network-based methods were used to resolve host-vector interactions and their relative importance in the segregating communities of haplotypes. Results Phylogenetic analyses resulted in 199 haplotypes within eight network-derived clusters, which were allocated to four ecotypes. The interactions of haplotypes between ticks, vertebrates and geographical origin, were visualized and quantified from networks. A high number of haplotypes were recorded in the tick Ixodes ricinus. Communities of A. phagocytophilum recorded from Korea, Japan, Far Eastern Russia, as well as those associated with rodents had no links with the larger set of isolates associated with I. ricinus, suggesting different evolutionary pressures. Rodents appeared to have a range of haplotypes associated with either Ixodes trianguliceps or Ixodes persulcatus and Ixodes pavlovskyi. Haplotypes found in rodents in Russia had low similarities with those recorded in rodents in other regions and shaped separate communities. Conclusions The groEl gene fragment of A. phagocytophilum provides information about spatial segregation and associations of haplotypes to particular vector-host interactions. Further research is needed to understand the circulation of this bacterium in the gap between Europe and Asia before the overview of the speciation features of this bacterium is complete. Environmental traits may also play a role in the evolution of A. phagocytophilum in ecotypes through yet unknown relationships.
    Anaplasma phagocytophilum evolves in geographical and biotic niches of vertebrates and ticks
    Jaarsma, Ryanne I. ; Sprong, Hein ; Takumi, Katsuhisa ; Kazimirova, Maria ; Silaghi, Cornelia ; Mysterud, Atle ; Rudolf, Ivo ; Beck, Relja ; Földvári, Gábor ; Tomassone, Laura ; Groenevelt, Margit ; Everts, Reinard R. ; Rijks, Jolianne M. ; Ecke, Frauke ; Hörnfeldt, Birger ; Modrý, David ; Majerová, Karolina ; Votýpka, Jan ; Estrada-Peña, Agustín - \ 2019
    Parasites & Vectors 12 (2019). - ISSN 1756-3305
    Anaplasma phagocytophilum - Ixodidae - Molecular epidemiology - Network analysis - Ticks - Transmission dynamics

    Background: Anaplasma phagocytophilum is currently regarded as a single species. However, molecular studies indicate that it can be subdivided into ecotypes, each with distinct but overlapping transmission cycle. Here, we evaluate the interactions between and within clusters of haplotypes of the bacterium isolated from vertebrates and ticks, using phylogenetic and network-based methods. Methods: The presence of A. phagocytophilum DNA was determined in ticks and vertebrate tissue samples. A fragment of the groEl gene was amplified and sequenced from qPCR-positive lysates. Additional groEl sequences from ticks and vertebrate reservoirs were obtained from GenBank and through literature searches, resulting in a dataset consisting of 1623 A. phagocytophilum field isolates. Phylogenetic analyses were used to infer clusters of haplotypes and to assess phylogenetic clustering of A. phagocytophilum in vertebrates or ticks. Network-based methods were used to resolve host-vector interactions and their relative importance in the segregating communities of haplotypes. Results: Phylogenetic analyses resulted in 199 haplotypes within eight network-derived clusters, which were allocated to four ecotypes. The interactions of haplotypes between ticks, vertebrates and geographical origin, were visualized and quantified from networks. A high number of haplotypes were recorded in the tick Ixodes ricinus. Communities of A. phagocytophilum recorded from Korea, Japan, Far Eastern Russia, as well as those associated with rodents had no links with the larger set of isolates associated with I. ricinus, suggesting different evolutionary pressures. Rodents appeared to have a range of haplotypes associated with either Ixodes trianguliceps or Ixodes persulcatus and Ixodes pavlovskyi. Haplotypes found in rodents in Russia had low similarities with those recorded in rodents in other regions and shaped separate communities. Conclusions: The groEl gene fragment of A. phagocytophilum provides information about spatial segregation and associations of haplotypes to particular vector-host interactions. Further research is needed to understand the circulation of this bacterium in the gap between Europe and Asia before the overview of the speciation features of this bacterium is complete. Environmental traits may also play a role in the evolution of A. phagocytophilum in ecotypes through yet unknown relationships.

    Domesticated equines differ in their faecal microbiota composition
    Edwards, J.E. ; Schennink, Angeline ; Burden, F. ; Long, S. ; Doorn, D.A. van; Pellikaan, W.F. ; Dijkstra, J. ; Saccenti, E. ; Everts, H. ; Smidt, H. - \ 2018
    - 1 p.
    Caprine in utero learning and feed neophobia
    Phan Vu, Hai - \ 2017
    Utrecht University. Promotor(en): W.H. Hendriks, co-promotor(en): J.Th. Schonewille; H. Everts. - Utrecht : Utrecht University - 126 p.
    Domesticated equines have fundamental differences in faecal microbial concentrations
    Edwards, J.E. ; Berg, Paul van den; Burden, F. ; Doorn, D.A. van; Pellikaan, W.F. ; Dijkstra, J. ; Everts, H. ; Smidt, H. - \ 2017
    - 2 p.
    Domesticated equines have fundamental differences in faecal microbial concentrations
    Edwards, J.E. ; Berg, Paul van den; Burden, F. ; Doorn, D.A. van; Pellikaan, W.F. ; Dijkstra, J. ; Everts, H. ; Smidt, H. - \ 2017
    Nutritional studies have shown that horses and donkeys differ, with donkeys having a higher dry matter digestibility (DMD) of dietary material and a longer gut retention time of dietary particles1,2. As analysis of the equine gut microbiome to date has primarily focussed on horses3, however, it is unclear to what extent these differences are mediated by differences in the physiology and/or hindgut microbiota of these domesticated equines (Figure 1). A preliminary study was therefore conducted in order to assess the faecal concentrations of bacteria, archaea and anaerobic fungi in horses, donkeys and hybrids (mules & hinnies) .
    Production and glyco-engineering of immunomodulatory helminth glycoproteins in plants
    Wilbers, Ruud H.P. ; Westerhof, Lotte B. ; Noort, Kim Van; Obieglo, Katja ; Driessen, Nicole N. ; Everts, Bart ; Gringhuis, Sonja I. ; Schramm, Gabriele ; Goverse, Aska ; Smant, Geert ; Bakker, Jaap ; Smits, Hermelijn H. ; Yazdanbakhsh, Maria ; Schots, Arjen ; Hokke, Cornelis H. - \ 2017
    Scientific Reports 7 (2017). - ISSN 2045-2322
    Helminth parasites control host-immune responses by secreting immunomodulatory glycoproteins. Clinical trials and mouse model studies have demonstrated the potential of helminth-derived glycoproteins for the treatment of immune-related diseases, like allergies and autoimmune diseases. Studies are however hampered by the limited availability of native parasite-derived proteins. Moreover, recombinant protein production systems have thus far been unable to reconstitute helminth-like glycosylation essential for the functionality of some helminth glycoproteins. Here we exploited the flexibility of the N-glycosylation machinery of plants to reconstruct the helminth glycoproteins omega-1 and kappa-5, two major constituents of immunomodulatory Schistosoma mansoni soluble egg antigens. Fine-tuning transient co-expression of specific glycosyltransferases in Nicotiana benthamiana enabled the synthesis of Lewis X (LeX) and LDN/LDN-F glycan motifs as found on natural omega-1 and kappa-5, respectively. In vitro and in vivo evaluation of the introduction of native LeX motifs on plant-produced omega-1 confirmed that LeX on omega-1 contributes to the glycoprotein’s Th2-inducing properties. These data indicate that mimicking the complex carbohydrate structures of helminths in plants is a promising strategy to allow targeted evaluation of therapeutic glycoproteins for the treatment of inflammatory disorders. In addition, our results offer perspectives for the development of effective anti-helminthic vaccines by reconstructing native parasite glycoprotein antigens.
    Mimicking immunomodulatory helminth glycoproteins in plants to enable treatment of inflammatory diseases
    Wilbers, R.H.P. ; Westerhof, L.B. ; Noort, Kim van; Obieglo, K. ; Driessen, N.N. ; Everts, B. ; Goverse, A. ; Smant, G. ; Bakker, J. ; Smits, H.H. ; Yazdanbakhsh, M. ; Schots, A. ; Hokke, C.H. - \ 2017
    Helminth parasites control host-immune responses by secreting immunomodulatory glycoproteins. Clinical trials and mouse model studies have demonstrated the potential of helminths and helminth-derived glycoproteins for the treatment of immune-related diseases, like allergies and autoimmune diseases. Studies are however hampered by the limited availability of native parasite-derived proteins. Moreover, recombinant protein production systems have thus far been unable to reconstitute helminth-like glycosylation essential for the functionality of helminth glycoproteins. Here we exploited the flexibility of the N-glycosylation machinery of plants to reconstruct two helminth glycoproteins, omega-1 and kappa-5, major constituents of immunomodulatory Schistosoma mansoni soluble egg antigens. Fine-tuning transient co-expression of specific glycosyltransferases in Nicotiana benthamiana enabled the synthesis of Lewis X (LeX) and LDN/LDN-F glycan motifs as found on natural omega-1 and kappa-5, respectively. In vitro and in vivo evaluation of the introduction of native LeX motifs on plant-produced omega-1 confirmed that LeX on omega-1 contributes to the glycoprotein’s Th2-inducing properties. These data indicate that mimicking the complex carbohydrate structures of helminths in plants is a promising strategy to allow targeted evaluation of therapeutic glycoproteins for the treatment of inflammatory disorders. In addition, our results offer perspectives for the development of effective anti-helminthic vaccines by reconstructing native parasite glycoprotein antigens.
    Anaerobic fungi are a key unexplored taxon for optimizing fibre utilisation in equines
    Edwards, J.E. ; Doorn, D.A. van; Pellikaan, W.F. ; Everts, H. ; Burden, F. ; Smidt, H. - \ 2016
    Anaerobic fungi are a key unexplored taxa for optimizing lignocellulosic fibre utilisation in equines
    Edwards, J.E. ; Doorn, D.A. van; Pellikaan, W.F. ; Dijkstra, J. ; Everts, H. ; Burden, F. ; Smidt, H. - \ 2016
    The hindgut microbiota of equines enables them to utilize forage/grazing based diets which contain a substantial proportion of lignocellulosic fibres. These fibres are a structural barrier that gut bacteria need to overcome when accessing plant nutrients, as well as being a challenging and structurally complex substrate that can be utilized. The limited dietary energy available from these ‘natural’ diets however means that many equines are supplemented with energy-dense concentrate feeds in order to fulfil their dietary energy requirements. Use of energy-dense concentrate feeds however can change the equine hindgut microbiome, and lead to the development of gut-mediated diseases (i.e. fermentative acidosis, laminitis, colic and stomach ulcers). There is therefore a clear need to optimize the utilization of lignocellulosic fibres in the equine hindgut in order to minimize the need for dietary supplementation. The most effective of the fibre-degrading gut microbes, anaerobic fungi (phylum Neocallimastigomycota), are known to be a normal member of the equine gut microbiota. Despite this however, they have been largely overlooked in equine gut microbiology studies to date. Research being conducted within the EU funded EQUIANFUN project will therefore establish baseline knowledge of the phylogeny, community structure, physiology and nutritional impact of anaerobic fungi in the equine hindgut. The insights gained will inform the development of novel strategies to promote indigenous anaerobic fungal communities in the equine hindgut, enabling optimization of the use of dietary forage as an energy source in equids. Reduction of the use of energy–dense diets and applying targeted nutritional strategies for optimizing microbial health may counteract processes in the gastrointestinal tract that have been associated with disease. Anaerobic fungi therefore offer the potential to enable significant advances to be made in the optimisation of the nutrition, health and welfare of all domesticated equids.
    Exposure to a novel feedstuff by goat dams during pregnancy and lactation versus pregnancy alone does not further improve post-weaning acceptance of this feedstuff by their kids
    Hai, P.V. ; Schonewille, J.T. ; Tien, D.V. ; Everts, H. ; Hendriks, W.H. - \ 2016
    Journal of the Science of Food and Agriculture 96 (2016)6. - ISSN 0022-5142 - p. 2215 - 2219.
    BACKGROUND
    Previous experiments demonstrated the existence of in utero learning in goats. However, in contrast to other animal species, in goats there is no information about the potential of flavour transmission from maternal feed to goat kids during lactation. The aim of the current study was to assess the role of post-natal exposure of Chromonaela odorata leaf meal (COLM) in relation to the preferences to this feedstuff by goat kids after weaning. It was hypothesised that exposure of COLM to the dams during both pregnancy and lactation versus pregnancy alone, additionally affects post-weaning intake of COLM by their offspring.
    RESULTS
    Consumption of COLM by the goat kids was similar during the first week post-weaning for all treatments. However, after 4 weeks the intake of COLM was at least 1.8 times greater when kids were exposed to COLM during pregnancy whereas it remained virtually unchanged when kids were exposed to COLM during lactation only. The increase in COLM consumption was in line with the observations on latency to eat and meal size.
    CONCLUSION
    Transmission of feeding behaviour from goat dams to offspring does not occur during lactation. However, the concept of in utero learning in goats was confirmed. © 2015 Society of Chemical Industry
    Engineering of plants for the expression of helminth glycoproteins with their native N-glycan structures
    Wilbers, Ruud - \ 2016
    Engineering plants for the expression of helminth glycoproteins with their native N-glycan structures Ruud H.P. Wilbers1, Lotte B. Westerhof 1, Bart Everts2, Kim van Noort 1, Debbie R. van Raaij1, Dieu-Linh Nguyen2, Maria Yazdanbakhsh2, Cornelis H. Hokke2 and Arjen Schots1 1 Laboratory of Nematology, Wageningen University, Wageningen, The Netherlands 2 Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands Schistosoma mansoni is a parasitic trematode that, like other helminths, secretes immunomodulatory proteins. These secreted proteins are main topics of research as they are possible vaccine candidates or may have therapeutic potential to treat inflammatory disorders. Many helminth secretory proteins carry complex N-glycans, but the exact role of these N-glycans on immunomodulatory properties remains to be elucidated. As the purification of a single glycoprotein from S. mansoni is inefficient and unsustainable, a platform is required that enables production of such glycoproteins. Here we show that S. mansoni-derived glycoproteins can be efficiently produced in plants. Furthermore, we have engineered the plant glycosylation machinery to synthesise N-glycans carrying structures like Lewis X or LDNF. Altogether, our results demonstrate that plants are an excellent platform for the expression of helminth glycoproteins with their native N-glycans. This opens up a new field of research and might lead to the identification of novel therapeutic targets.
    Engineering of plants for the expression of helminth glycoproteins with their native N-glycan structures
    Wilbers, R.H.P. ; Westerhof, L.B. ; Noort, Kim van; Nguyen, D.L. ; Smant, G. ; Bakker, J. ; Hokke, C.H. ; Schots, A. - \ 2016
    - 1 p.
    Engineering plants for the expression of helminth glycoproteins with their native N-glycan structures


    Ruud H.P. Wilbers1, Lotte B. Westerhof 1, Bart Everts2, Kim van Noort 1, Debbie R. van Raaij1, Dieu-Linh Nguyen2, Maria Yazdanbakhsh2, Cornelis H. Hokke2 and Arjen Schots1

    1 Laboratory of Nematology, Wageningen University, Wageningen, The Netherlands
    2 Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands


    Schistosoma mansoni is a parasitic trematode that, like other helminths, secretes immunomodulatory proteins. These secreted proteins are main topics of research as they are possible vaccine candidates or may have therapeutic potential to treat inflammatory disorders. Many helminth secretory proteins carry complex N-glycans, but the exact role of these N-glycans on immunomodulatory properties remains to be elucidated. As the purification of a single glycoprotein from S. mansoni is inefficient and unsustainable, a platform is required that enables production of such glycoproteins. Here we show that S. mansoni-derived glycoproteins can be efficiently produced in plants. Furthermore, we have engineered the plant glycosylation machinery to synthesise N-glycans carrying structures like Lewis X or LDNF. Altogether, our results demonstrate that plants are an excellent platform for the expression of helminth glycoproteins with their native N-glycans. This opens up a new field of research and might lead to the identification of novel therapeutic targets.
    Energy requirement for maintenance in growing pigs
    Everts, H. - \ 2015
    Wageningen : WUR Livestock Research, Dept. Animal Nutrition (CVB-Documentation report nr. 57) - 19
    A new Dutch Net Energy formula for feed and feedstuffs for growing and fattening pigs
    Blok, M.C. ; Brandsma, Gerard ; Bosch, G. ; Gerrits, W.J.J. ; Jansman, A.J.M. ; Everts, H. - \ 2015
    Wageningen UR Livestock Research (CVB-Documentation report 56) - 39
    Veen verschijnt en verdwijnt : Grondwaterstromen en veenvorming
    Makaske, A. ; Maas, G.J. ; Grootjans, A. ; Meijles, Erik ; Everts, H. ; Vries, Nico de - \ 2015
    In: Landschapsbiografie van de Drentsche Aa / Spek, Theo, Elerie, Hans, Bakker, Jan P., Noordhoff, Ineke, Assen : Koninklijke Van Gorcum - ISBN 9789023252719 - p. 54 - 81.
    Het geïntegreerde onderzoek van de laatste jaren laat zien dat het landschap van de Drentsche Aa zeer veranderlijk is. Wie de reconstructie van de tweede levensfase van het Drentsche Aa-gebied naast die uit de ijstijd legt, ziet dat ook direct. Er zijn aanwijzingen dat het landschap in het Atlanticum flink bebost is, alleen vlak langs beken ligt open veen. Later lijken de bomen het loodje te leggen. Ook de broekbossen die later zijn ontstaan hebben niet het eeuwige leven en ook de wouden uit de Middeleeuwen verdwijnen weer.
    Engineering plants for the expression of helminth glycoproteins with their native N-glycan structures
    Wilbers, R.H.P. ; Westerhof, L.B. ; Everts, B. ; Noort, Kim van; Raaij, D.R. van; Nguyen, D. ; Yazdanbakhsh, M. ; Hokke, C.H. ; Schots, A. - \ 2015
    Schistosoma mansoni is a parasitic trematode that, like other helminths, secretes immunomodulatory proteins. These secreted proteins are main topics of research as they are possible vaccine candidates or may have therapeutic potential to treat inflammatory disorders. Many helminth secretory proteins carry complex N-glycans, but the exact role of these N-glycans on immunomodulatory properties remains to be elucidated. As the purification of a single glycoprotein from S. mansoni is inefficient and unsustainable, a platform is required that enables production of such glycoproteins. Here we show that S. mansoni-derived glycoproteins can be efficiently produced in plants. Furthermore, we have engineered the plant glycosylation machinery to synthesise N-glycans carrying structures like Lewis X or LDNF. Altogether, our results demonstrate that plants are an excellent platform for the expression of helminth glycoproteins with their native N-glycans. This opens up a new field of research and might lead to the identification of novel therapeutic targets.
    Daily methane production pattern of Welsh ponies fed a roughage diet with or without a cereal mixture
    Dansen, O. ; Pellikaan, W.F. ; Hendriks, W.H. ; Dijkstra, J. ; Jacobs, M.P.T. ; Everts, H. ; Doorn, D.A. van - \ 2015
    Journal of Animal Science 93 (2015)4. - ISSN 0021-8812 - p. 1916 - 1922.
    lactating dairy-cows - detergent fiber - enteric methane - horses - digestibility - cattle - performance - physiology - ruminants - nutrition
    Methane production from Welsh ponies fed 2 isoenergetic diets (NE basis) at maintenance was studied in a crossover design with 4 mature geldings (230 ± 10.5 kg BW, mean ± SE). Treatments included a roughage-only (R) diet (5.1 kg DM/d) or a roughage plus cereal mix (RC) diet (2.5 kg DM hay/d plus 1.1 kg DM cereal mix/d). For both diets, the same grass hay was used (898 g DM/kg and 4.5 MJ NEm/kg DM) and a commercial cereal mix was used in the RC diet (890 g DM/kg and 9.6 MJ NEm/kg DM). Ponies were housed in pairs in climate-controlled respiration chambers. Carbon dioxide production (CO2), oxygen (O2) consumption, and CH4 production were measured over 3 consecutive days. Heat production (HP) rates were calculated from gaseous exchange. Feces were collected quantitatively to determine dietary nutrient digestibility. Dry matter intake differed between diets (P <0.0001), but NE intake was equal for both diets (22.3 ± 0.07 MJ NEm/d). Organic matter digestibility was lower (P = 0.006) for the R diet (47.2%) than the RC diet (55.6%). Methane production was higher (P = 0.014) on the R diet (29.8 L·pony–1·d–1) compared to the RC diet (23.2 L·pony–1·d–1). Methane production expressed in liters/kilogram metabolic body weight (BW0.75) per day tended (P = 0.064) to decrease with 21% for the RC group compared with the R group. Heat production, O2 consumption, and CO2 production were not affected by diet. Diurnal patterns of CH4 production and HP were similar for both diets. Methane production increased slightly (P <0.652) after feeding and was numerically lower for the RC diet for all time points throughout the day. For both diets, HP was higher after feeding than before feeding and decreased again within approximately 3 h after feeding. Isoenergetic replacement of roughage by a cereal mix reduces CH4 production in ponies. No clear diurnal pattern in CH4 emission can be discerned in ponies fed at maintenance
    Factors contributing to the variation in feline urinary oxalate excretion
    Dijcker, J.C. ; Hagen-Plantinga, E.A. ; Everts, H. ; Queau, Y. ; Biourge, V.C. ; Hendriks, W.H. - \ 2014
    Journal of Animal Science 92 (2014)3. - ISSN 0021-8812 - p. 1029 - 1036.
    glycolate excretion - dietary-protein - ascorbic-acid - calcium - cats - hydroxyproline - vitamin-b-6 - fructose - requirement - absorption
    This study aimed to identify factors (season, animal, and diet) contributing to the variation in urinary oxalate (Uox) excretion rate, Uox concentration, and urine volume in healthy adult cats. A data set (1,940 observations) containing information on Uox excretion rate of 65 cats fed 252 diets (i.e., each diet was fed to a group of 6 to 8 cats), with known dietary oxalate concentrations, collected over a 6 yr period at a feline nutrition facility, were retrospectively analyzed. Data related to season, animal (i.e., age, gender, body weight, and breed), and diet (i.e., nutrient content) characteristics were subjected to stepwise multivariate regression analysis to identify factors significantly correlated to Uox excretion rate (µmol/(kg BW0.67·d)) and concentration (mmol/L) as well as urine volume (mL/(kg BW0.67·d)). Independent factors significantly (P <0.05) associated with lower Uox concentration (mmol/L) included greater ash, Ca, and Na intake and lower nitrogen-free extract, total dietary fiber, P, and oxalate intake, and a body weight
    Improved acceptance of Chromonaela odorata by goat kids after weaning is caused by in utero exposure during late but not early pregnancy
    Hai, P.V. ; Schonewille, J.T. ; Tien, D.V. ; Everts, H. ; Hendriks, W.H. - \ 2014
    Applied Animal Behaviour Science 159 (2014). - ISSN 0168-1591 - p. 50 - 54.
    feeding-behavior - fetal sheep - chromolaena-odorata - food preference - amniotic-fluid - maternal diet - milk - consumption - fetus - rat
    The aim of the current experiment was to study the effect of the phase of pregnancy on in utero learning of Chromonaela odorata by the goat kids by comparing mid pregnancy (day 50–99, MP) with late pregnancy (day 100–145, LP). It was hypothesized that kids born to dams fed C. odorata during late pregnancy (day 100–145) would show an improved post-weaning consumption of this plant. Twenty four female goats (Co breed) were synchronized, inseminated and divided randomly into 4 equal groups. All pregnant goats were fed a diet either without (control) or with 50 g of C. odorata leave meal (COLM) at 10:00 am during 30 min during mid and late pregnancy. The COLM diet was fed either from 50 to 99 days of pregnancy (mid pregnancy, MP), or from 100 to 145 days of pregnancy (late pregnancy, LP) or from 50 to 145 days of pregnancy (MLP, positive control). After weaning (3 months old), one kid from each goat dam was selected to measure COLM intake for 30 min over a 4-week period. Feeding activities of the individually housed goat kids were monitored with a camera system. Post-weaning consumptions of COLM by the goat kids increased significantly (P <0.05) in the LP and MLP treatments and remained essentially unchanged in the control and MP treatments. The higher consumption of COLM by kids from the LP and MLP treatment was associated with a significantly (P <0.05) shorter latency to eat and a longer chewing time (P <0.05). It was concluded that transmission of feeding behaviour from mother to offspring occurs between day 100 to 145 of gestation and that it remains present at least 3 months after weaning in goats
    Hydrate sodium calcium aluminosilicate does not reduce rumen lipopolysacharide concentrations in cows
    Pilachai, R. ; Schonewille, J.T. ; Thamrongyoswittayakul, C. ; Aiumlamai, S. ; Wachirapakorn, C. ; Everts, H. ; Vlaeminck, B. ; Doekes, G. ; Hendriks, W.H. - \ 2014
    Animal Feed Science and Technology 191 (2014). - ISSN 0377-8401 - p. 111 - 115.
    subacute ruminal acidosis - dairy-cows - inflammatory response - in-vitro - lipopolysaccharide - smectite - triggers - system - milk
    The efficacy of hydrate sodium calcium aluminosilicate (HSCAS) to reduce the concentrations of free lipopolysaccharide (LPS) in rumen fluid of cows was investigated. Six, rumen-fistulated crossbred Holstein, non-pregnant, dry cows were randomly assigned to three experimental rations in a study with a replicated 3 × 3 Latin square design with 28-d periods. During the first 20 days of each experimental period, cows were offered concentrate (5.4 kg dry matter; DM) without HSCAS and rice straw ad libitum. On day 21, cows were fasted for 12 h prior to commencement of a 7-d measurement period during which time cows were offered concentrate (10.5 kg DM) containing either 0, 5 or 10 g/kg of HSCAS and 1.5 kg DM rice straw. Rumen fluid was collected on days 1, 3 and 7 during the measurement period for analysis of pH, LPS and volatile fatty acids (VFA). Supplemental HSCAS did not affect the pH or the concentrations of total and individual VFA in the ruminal fluid. Mean postprandial rumen pH was reduced to values below 5.6 at all times. Rumen LPS concentrations significantly increased from day 1 to 7 during each measurement period with values ranging from 4489 to 104,000 EU/mL but they were not significantly affected by supplemental HSCAS.
    Diet factors and subclinical laminitis score in lactating cows of smallholder dairy farms in Thailand
    Pilachai, R. ; Schonewille, J.T. ; Thamrongyoswittayakul, C. ; Aiumlamai, S. ; Wachirapakom, C. ; Everts, H. ; Hendriks, W.H. - \ 2013
    Livestock Science 155 (2013)2-3. - ISSN 1871-1413 - p. 197 - 204.
    physically effective fiber - neutral detergent fiber - ruminal ph - milk-yield - cattle - forage - rumen - protein - lesions - digestibility
    The objective of this study was to evaluate the importance of dietary crude protein (CP) content, dietary neutral detergent fiber (NDF) content and feeding regime as well as other factors related to management and demographics on the occurrence of (subclinical) laminitis under practical Thai feeding conditions. Hemorrhage of the white line and the sole, sole ulcer and white-line fissure of all four claws of milking cows (n=119) on 25 farms (selected based on the occurrence of lameness) were macroscopically assessed to calculate the prevalence of subclinical laminitis (SCL) on each farm. Data were collected on farm characteristics, feed and feeding management, floor type and hoof care. Dry matter intake was assessed on each farm and feed ingredients collected and analyzed for dry matter (DM), CP and NDF. No significant differences were found for farm characteristics such as herd size, number of milking cows, parity and body condition scoring between farms with a low (25%) of SCL. Percentages of DM and CP content of the rations did not differ, whereas mean NDF content in the ration was significantly higher in the low compared to the high prevalence farms. Multiple regression analysis of the data showed that a ration low in NDF content and/or in combination with the separate feeding of roughage and concentrate was associated with a high SCL prevalence. The results suggest that mixing concentrate with a substantial part of the roughage is an important strategy to prevent SCL in smallholder dairy farms under Thai feeding conditions. In addition, the dietary NDF content but not the dietary CP level is associated with SCL prevalence in dairy cows under Thai feeding conditions.
    Improved acceptance of Chromonaela odorata by goat kids after weaning is triggered by in utero exposure but nog consumption of milk
    Hai, P.V. ; Schonewille, J.T. ; Tien, D.V. ; Everts, H. ; Hendriks, W.H. - \ 2013
    Applied Animal Behaviour Science 146 (2013). - ISSN 0168-1591 - p. 66 - 71.
    chromolaena-odorata - feeding-behavior - food preference - amniotic-fluid - maternal diet - tropical weed - pregnant ewes - fetal - sheep - taste
    The aim of the current study was to determine whether the improved post-weaning intake of Chromonaela odorata by goat kids is related to either the in utero period of the goat kids or the subsequent suckling period. It was hypothesized that kids born to dams fed C. odorata during pregnancy and receiving milk from dams not exposed to C. odorata during pregnancy show an improved acceptance to consume this plant. Twenty female goats were successfully synchronized and divided into 4 groups. Two groups (1 and 3) were offered 50 g of dried C. odorata leave meal (COLM) mixed with a basic diet for the last 3 months of pregnancy until 1 week before parturition. At birth the kids from the goats in group 1 and 2 were cross fostered without colostrum or milk from their own mother. While waiting for the delivery of kid from another goat, the kid was fed milk replacer or milk from any goat dam in the same treatment. Kids from groups 3 and 4 remained with their mothers. After weaning (2.5 months old), one kid from each goat dam was selected for COLM intake which was measured for 30 min over a 4 week period. Feeding activities of the individually housed goat kids was monitored with a camera system. Kids born to dams receiving COLM during pregnancy consumed higher amounts of the COLM supplemented test feed during all feeding preference tests compared to kids from the control group, particularly during week 3 and 4 (P <0.001). Shorter latency, longer time spent on each meal and total eating time, chewing time and higher meal size (P <0.05) were different in the kids born from does that ingested COLM during pregnancy. It is concluded that prenatal exposure to C. odorata via maternal ingestion significantly increases the intake of C. odorata by weaned goat kids. This improved intake is due to the in utero learning and not the transfer of (secondary) components via the milk of the mothers fed C. odorata during pregnancy.
    Methane production in warmblood ponies fed either a roughage only or a roughage plus concentrate diet
    Dansen, O. ; Pellikaan, W.F. ; Hendriks, W.H. ; Dijkstra, J. ; Jacobs, M.P.T. ; Everts, H. ; Doorn, D.A. van - \ 2013
    In: Proceedings of the 5th Greenhouse Gases and Animal Agriculture Conference (GGAA2013). - Cambridge : Cambridge University Press - ISBN 9780906562697 - p. 517 - 517.
    Methane production in warmblood ponies fed either a roughage or a roughage plus concentrate ration
    Dansen, O. ; Pellikaan, W.F. ; Hendriks, W.H. ; Dijkstra, J. ; Jacobs, M.P.T. ; Everts, H. ; Doorn, D.A. van - \ 2013
    In: Proceedings of the 6th Edition Horse Health Nutrition - European Equine Health & Nutrition Congress. - EEHNC - p. 176 - 176.
    Mineralen-, sporenelementen- en vitaminenbehoeften van paarden
    Top, A.M. van den; Blok, M.C. ; Everts, H. - \ 2012
    Lelystad : CVB, Productschap Diervoeder (CVB-documentatierapport nr. 54) - 68
    paarden - diervoeding - voeding - voedering - voedingsstoffen - mineralenvoeding - sporenelementen - vitaminen - horses - animal nutrition - nutrition - feeding - nutrients - mineral nutrition - trace elements - vitamins
    In deze publicatie wordt beschreven hoe de in CVB verband tot stand gekomen voedernormen voor deze voedingsstoffen tot stand zijn gekomen. De publicatie is het resultaat van een deskstudie waarbij allereerst de Duitse DLG normen en de Amerikaanse NRC normen zijn geëvalueerd. Daar waar nodig is gericht gekeken of er nieuwe studies beschikbaar zijn, of is teruggegrepen op de oorspronkelijke publicaties. Voor de macromineralen (calcium, fosfor, magnesium, natrium, kalium, chloor) zijn de voedernormen gebaseerd op de zgn. factoriële methode. Voor sporenelementen en de vitamines bleek deze benadering niet mogelijk. Het rapport bevat een groot aantal tabellen waarin de voedernormen voor de genoemde nutriënten voor alle relevante fysiologische stadia.
    The effects of high levels of rumen degradable protein on rumen pH and histamine concentrations in dairy cows
    Pilachai, R. ; Schonewille, J.T. ; Thamrongyoswittayakul, C. ; Aiumlamai, S. ; Wachirapakom, C. ; Everts, H. ; Hendriks, W.H. - \ 2012
    Journal of Animal Physiology and Animal Nutrition 96 (2012)2. - ISSN 0931-2439 - p. 206 - 213.
    ruminal degradability - low-quality - acidosis - carbohydrate - cattle - fermentation - laminitis - digestion - histidine - system
    An experiment was conducted to test the hypothesis that the supplementation of crude protein (CP) results in rumen acidosis and increased histamine concentrations in dairy cows. Six ruminally fistulated, non-pregnant dry cows were fed three experimental rations in a replicated 3 × 3 Latin square design. The CP contents in the low-CP, the high rumen undegradable protein (high-RUP) and the high rumen degradable protein (high-RDP) rations were 112, 259 and 266 g/kg dry matter (DM) respectively. The cows were fed 7.7 kg DM of the concentrates and 2.7 kg DM of rice straw. High levels of RDP in the ration significantly increased the ammonia, total volatile fatty acid (VFA) and histamine concentrations in the rumen fluid. However, supplemental CP, whether degradable or undegradable, did not significantly affect the pH of rumen fluid. Plasma urea nitrogen concentration was higher in both high-RDP and high-RUP in relation to cows fed the low-CP ration. The rise in ruminal histamine concentrations was physiologically non-relevant, most likely because rumen pH was not affected by supplemental CP at the installed level of DM intake. Therefore, it can be concluded that the issue of supplemental CP, rumen pH and ruminal histamine concentrations has not yet been settled. Further research is warranted to understand these relationships.
    Feeding Chromonaela odorata during pregnancy to goat dams affects acceptance of this feedstuff by their offspring
    Hai, P.V. ; Everts, H. ; Tien, D. van; Schonewille, J.T. ; Hendriks, W.H. - \ 2012
    Applied Animal Behaviour Science 137 (2012)1-2. - ISSN 0168-1591 - p. 30 - 35.
    food preference - chromolaena-odorata - sensitive period - amniotic-fluid - maternal diet - tropical weed - behavior - lambs - exposure - flavor
    We investigated the effect of Chromonaela odorata ingestion by goat dams during pregnancy on intake of C. odorata by their kids. Alteration of prenatal feed preference may be used to increase feed intake of novel feeds and the transit from outdoor to indoor goat farming in Vietnam. Ten female goats were synchronized, inseminated and divided randomly into 2 equal groups. The experimental group was offered 50 g of sun dried C. odorata leave meal (COLM) along with a basal diet from day 45 to 135 of pregnancy while the control group was offered only the basal diet during this period. After weaning (3 mo), one kid from each goat dam in both groups was randomly selected and housed in individual cages. After a 9.5 h fast, each kid was offered 50 g of COLM daily at 8:30 am for 30 min for 4 weeks. Feeding activities were monitored with a video camera system. We determined mean COLM intake and indices of feed acceptance including latency to eat, number of visits without intake, meal frequency, eating bout length, eating time, intake rate, meal size and chewing time for each kid. Intake of COLM by goat kids born to dams fed COLM was significantly higher over the 4 weeks compared to goat kids born to dams not fed COLM (11.32 ± 3.60 g vs. 6.09 ± 1.91 g, P <0.028). This difference was more pronounced during weeks 3 and 4 than weeks 1 and 2. Goat kids from mothers fed COLM had a shorter latency to eat, a larger meal size and a longer chewing time than kids from mothers not fed COLM during pregnancy. We conclude that in utero transmission of feeding preference occurred from does to their offspring.
    Starch source in high concentrate rations does not affect rumen pH, histamine and lipopolysaccharide concentrations in dairy cows
    Pilachai, R. ; Schonewille, J.T. ; Thamrongyoswittayakul, C. ; Aiumlamai, S. ; Wachirapakom, C. ; Everts, H. ; Hendriks, W.H. - \ 2012
    Livestock Science 150 (2012)1-3. - ISSN 1871-1413 - p. 135 - 142.
    subacute ruminal acidosis - induced lactic-acidosis - inflammatory response - cattle - fermentation - endotoxin - degradation - performance - steers - fluid
    The replacement of ground corn by cassava meal on rumen pH, lipopolysaccharide (LPS) and histamine concentrations under typical Thai feeding conditions (high concentrate diets and rice straw as the sole source of roughage) was investigated. Four rumen-fistulated crossbred Holstein, non-pregnant, dry cows were randomly assigned to the four experimental rations in a 4×4 Latin square design with 21-d periods. Each period consisted of a 14-d run-in/wash out period, followed by a 7-d experimental period. During the run-in/wash out period, the cows were offered 5.4 kg DM of concentrate containing 4.6% cassava meal and rice straw was provided ad libitum. During the 7 day of each experimental period, cows were offered 10.5 kg DM of concentrate containing either 4.6% or 18.3% or 32.4% or 46.2% cassava meal and 1.5 kg of rice straw. Irrespective of dietary treatments, rumen total VFA and lactate concentrations were significantly affected by sampling day, which were significantly higher on day 1 versus days 3 and 7 of the experimental periods. Mean postprandial rumen pH was reduced to values
    Dietary and animal-related factors associated with the rate of urinary oxalate and calcium excretion in dogs and cats
    Dijcker, J.C. ; Hagen-Plantinga, E.A. ; Everts, H. ; Bosch, G. ; Kema, I.P. ; Hendriks, W.H. - \ 2012
    Veterinary Record 171 (2012)2. - ISSN 0042-4900 - 7 p.
    ligament insufficiency - diagnostic-accuracy - arthrography
    This paper reports the results of a cohort study and randomised clinical trial (RCT) in cross-over design. In the cohort study, the range of urinary oxalate (Uox) and calcium (Uca) excretion was determined within a sample of the Dutch population of dogs and cats, and dietary and animal-related factors associated with these urine parameters were identified. Spot urine samples were collected from privately owned dogs (n=141) and cats (n=50). The RCT determined the effect of a commercial raw meat diet versus a dry diet on Uox and Uca excretion rate in 23 dogs. In the cohort study, Uox excretion ranged from 21.1 to 170.6 mmol oxalate/mol creatinine in dogs and 27.5 to 161.6 in cats. Urinary calcium excretion ranged from 3.4 to 462.8 mmol calcium/mol creatinine in dogs and 10.1 to 128.0 in cats. In dogs, increased Uox and Uca excretion was associated with (1) the intake of a dry diet as the primary source of energy, (2) receiving no snacks and (3) breed. Increased Uox excretion was associated with males as well. In cats, urine collection in anaesthetised subjects was identified as a confounder. In the RCT, feeding the dry diet resulted in higher Uox (P
    Guest Editorial: Practising emotions
    Everts, J. ; Wagner, L.B. - \ 2012
    Emotion, Space and Society 5 (2012)3. - ISSN 1755-4586 - p. 174 - 176.
    Raw meat diet reduces urinary oxalate and calcium excretion rate in dogs
    Dijcker, J.C. ; Hagen-Plantinga, E.A. ; Everts, H. ; Bosch, G. ; Kema, I.P. ; Hendriks, W.H. - \ 2012
    In: Proceedings of the 37th Animal Nutrition Research Forum, Wageningen, The Netherlands, 18 April 2012. - Wageningen : Wageningen University - p. 11 - 12.
    Restoration of Wet Dune Slacks on the Dutch Wadden Sea Islands: Recolonization After Large-Scale Sod Cutting
    Grootjans, A.P. ; Everts, H. ; Bruin, K. ; Fresco, L. - \ 2011
    Restoration Ecology 9 (2011)2. - ISSN 1061-2971 - p. 137 - 146.
    The effects of sod cutting were studied in a dune area on the Dutch Wadden Sea Island of Texel. Sod cutting was carried out in a range of different dune slacks in order to restore dune slack vegetation with many endangered Red List species. Sod cutting removed approximately 96% of the soil seed bank. Species abundant in the seed bank, notably Juncacea, also had a high frequency in the vegetation that established during the first year after the restoration measures. Many other species not registered in the seed bank or in the former vegetation also appeared. Species richness in the monitored plots exceeded that of uncut reference plots after a few years. Colonization rates were higher than extinction rates in most plots, indicating that a stable state has not been reached after 5 years. Differences in species richness between slacks appeared to be related to the occurrence of source areas nearby and availability of dispersal agents, such as flooding and animals.
    Effect of different additives on ensiling Leucaena Leucocephala
    Wongsanit, Jaturong ; Schonewille, J.T. ; Rukkhamsuk, Theera ; Everts, H. ; Hendriks, W.H. - \ 2010
    - p. 2017 - 2018.
    The effect of variable proportions rapid degradable carbohydrate on rumen pH and endotoxin concentrations in diary cows
    Pilachai, R. ; Schonewille, J.T. ; Chaiyotwittayakun, A. ; Wachirapakorn, C. ; Everts, H. ; Hendriks, W.H. - \ 2010
    - p. 226 - 227.
    The effects of feeding chromonaela odorata to goat dams during pregnancy on better acceptance of this feedstuff by their offspring: learning in utero or by consuming milk
    Hai, P.V. ; Schonewille, J.T. ; Tien, D.V. ; Everts, H. ; Hendriks, W.H. - \ 2010
    Vitamin B12 Deficiency Stimulates Osteoclastogenesis via Increased Homocysteine and Methylmalonic Acid
    Vaes, B.L.T. ; Lute, C. ; Blom, H.J. ; Bravenboer, N. ; Vries, T.J. de; Everts, V. ; Dhonukshe-Rutten, R.A.M. ; Müller, M.R. ; Groot, C.P.G.M. de; Steegenga, W.T. - \ 2009
    Calcified Tissue International 84 (2009)5. - ISSN 0171-967X - p. 413 - 422.
    bone-mineral density - randomized controlled-trial - postmenopausal women - in-vitro - plasma homocysteine - pernicious-anemia - turnover markers - older persons - elderly-women - hip fracture
    The risk of nutrient deficiencies increases with age in our modern Western society, and vitamin B12 deficiency is especially prevalent in the elderly and causes increased homocysteine (Hcy) and methylmalonic acid (MMA) levels. These three factors have been recognized as risk factors for reduced bone mineral density and increased fracture risk, though mechanistic evidence is still lacking. In the present study, we investigated the influence of B12, Hcy, and MMA on differentiation and activity of bone cells. B12 deficiency did not affect the onset of osteoblast differentiation, maturation, matrix mineralization, or adipocyte differentiation from human mesenchymal stem cells (hMSCs). B12 deficiency caused an increase in the secretion of Hcy and MMA into the culture medium by osteoblasts, but Hcy and MMA appeared to have no effect on hMSC osteoblast differentiation. We further studied the effect of B12, Hcy, and MMA on the formation of multinucleated tartrate-resistant acid phosphatase¿positive osteoclasts from mouse bone marrow. We observed that B12 did not show an effect on osteoclastogenesis. However, Hcy as well as MMA were found to induce osteoclastogenesis in a dose-dependent manner. On the basis of these results, we conclude that B12 deficiency may lead to decreased bone mass by increased osteoclast formation due to increased MMA and Hcy levels.
    Restauratie van schraallanden op veengronden door afgraven en vernatten [thema restauratie van natte schraallanden]
    Grootjans, A. ; Kemmers, R.H. ; Everts, H. ; Adema, E. - \ 2007
    De Levende Natuur 108 (2007)3. - ISSN 0024-1520 - p. 108 - 113.
    veengronden - bodemchemie - vegetatie - herstel - hooiland - hydrologie - plaggen steken - beekdalen - herstelbeheer - friesland - drenthe - peat soils - soil chemistry - vegetation - rehabilitation - meadows - hydrology - sod cutting - brook valleys - restoration management - friesland - drenthe
    Verdroging van veengronden is een veel voorkomend verschijnsel in beekdalen. Hoewel hydrologisch herstel een eerste vereiste is, zijn aanvullende maatregelen wenselijk. In deze obn bijdrage wordt verslag gedaan van ervaringen met het plaggen in De Lage Maden (Drentse Aa), De Barten (Linde) en De Wyldlannen (lage midden, Friesland)
    Monstername en analyse van N-mineraal in de bodem en nitraat in het grondwater
    Hoving, I.E. ; Everts, H. ; Chardon, W.J. - \ 2005
    Lelystad : Animal Sciences Group (Reeks sturen op nitraat 14) - 38
    nitraten - stikstof - bodemwater - bodem - bemonsteren - analyse - landbouw - nederland - nitrates - nitrogen - soil water - soil - sampling - analysis - agriculture - netherlands
    Beschrijving methoden en -technieken van bemonstering bodem op N-mineraal en grondwater op nitraat toegepast in het project Sturen Op Nitraat
    Health and growth of veal calves fed milk replacers with or without probiotics
    Timmerman, H.M. ; Mulder, L. ; Everts, H. ; Espen, D.C. van; Wal, E. van der; Klaassen, G. ; Rouwers, S.M.G. ; Hartemink, R. ; Rombouts, F.M. ; Beynen, A.C. - \ 2005
    Journal of Dairy Science 88 (2005)6. - ISSN 0022-0302 - p. 2154 - 2165.
    antibiotic-associated diarrhea - lactic-acid bacteria - lactobacillus-gg - prevention - infection - stress - supplementation - malabsorption - typhimurium - performance
    Four experiments with 1-wk-old veal calves were conducted to assess the influence of probiotics on growth and health indicators. In experiments 1 and 2, the liquid probiotic supplements were administered daily from experimental d 1 to 15. The treatment period in experiments 3 and 4 was extended to 56 d. The probiotics used were a multispecies probiotic (MSPB) containing different probiotic species of human origin, or a calf-specific probiotic (CSPB) containing 6 Lactobacillus species isolated from calf feces and selected on the basis of a combination of characteristics. When the data for the 4 experiments were pooled, the probiotics enhanced growth rate during the first 2 wk. During the 8-wk experimental period, average daily gain and feed efficiency were significantly improved in the probiotic-treated groups. The MSPB-induced increase in weight gain was greater when the control calves were considered less healthy based on a health score (an index of diarrhea and therapeutic treatments). Probiotic treatment tended to diminish mortality. The CSPB treatment reduced the incidence of diarrhea and the fecal counts of coliforms. When therapeutic treatment was intensive in the control calves, the ingestion of probiotics reduced the percentage of calves that required therapy and the amount of treatments needed against digestive or respiratory diseases. There was no clear difference in the efficiency of the MSPB and CSPB preparations. Further research is necessary to identify underlying mechanisms and to evaluate the potential of probiotics to improve respiratory health in veal calf production.
    Muscle development of livestock animals: physiology, genetics and meat quality
    Pas, M.F.W. Te; Everts, M.E. ; Haagsman, H.P. - \ 2004
    CABI - ISBN 9780851998114 - 411 p.
    This book describes the development, growth and adaptation of livestock muscle tissue and contains 18 chapters divided into physiology, genetics and meat quality sections. The physiology section contains chapters on the mechanism of muscle fibre development in the fetus and the importance of high muscle fibre numbers for muscle mass and meat quality (1); muscle fibre type identification and characterization in livestock (2); manipulation of muscle fibre number during prenatal development (3); the effect of growth and exercise on muscle characteristics in relation to meat quality (4); implications of nutrition, hormone receptor expression and gene interactions for muscle development and disease (5); the impact of minerals and micronutrients on growth control (6); significance of exercise and thyroid hormones for development and performance (7); local and systemic regulation of muscle growth (8) and proteolytic systems and regulation of muscle remodelling and breakdown (9). The genetics section contains chapters on the muscle regulatory factors gene family in relation to meat production (10); the muscle transcriptome (11); genome analysis of quantitative trait loci for muscle tissue development and meat quality (12); functional genomics and proteomics in relation to muscle tissue (13); role of myostatin in muscle growth (14) and the genetics, physiology and meat quality aspects of the Callipyge mutation for sheep muscular hypertrophy (15). The meat quality section contains chapters on the genetic control of intramuscular fat accretion (16); postmortem muscle proteolysis and meat tenderness (17) and the water holding capacity of meat (18). Each chapter ends with a list of references and an index is located at the end of the book. This book will be of value for those interested in skeletal muscle biology and meat quality.
    Nitrogen balance of growing mink (Mustela Vison): Comparison of the balance period and the comparative slaughter technique
    Hissink, H.H.A.L. ; Verstegen, M.W.A. ; Everts, H. - \ 2004
    Individually assessed creep food consumption by suckled piglets: influence on post-weaning food intake characteristics and indicators of gut structure and hind-gut fermentation
    Bruininx, E.M.A.M. ; Schellingerhout, A.B. ; Binnendijk, G.P. ; Peet-Schwering, C.M.C. van der; Schrama, J.W. ; Hartog, L.A. den; Everts, H. ; Beynen, A.C. - \ 2004
    Animal Science 78 (2004)1. - ISSN 1357-7298 - p. 67 - 75.
    housed weanling pigs - feed-intake characteristics - villous height - crypt depth - weaned pigs - performance - weight - growth - diet
    Individual food intake characteristics and indicators of gut physiology of group-housed weanling pigs were measured in relation to pre-weaning consumption of creep food. Additionally, the effects of creep food consumption on pre-weaning body weight and gain were assessed. A total of 48 litters was used in two trials. From 11 days of age until weaning (day 28), all 48 litters were given a creep food (12.7 MJ net energy (NE) per kg, 15.2 g lysine per kg) supplemented with 10 g chromium III oxide per kg. Piglets showing green-coloured faeces on three sampling days were designated as good eaters, whereas piglets that never showed green faeces were labelled as non-eaters. Piglets having green faeces once or twice were designated as moderate eaters. Based on availability, body weight, litter origin, genotype and gender 29 good eaters, 32 moderate eaters and 29 non-eaters were selected in the first trial. In the second trial there were 30 good eaters, 33 moderate eaters, and 27 non-eaters. In each trial eight piglets of each creep-food eating type were immediately killed to serve as a reference group. The remaining piglets of each eating type were weaned and placed in pens equipped with computerized feeding stations so that distributions of body weight, litter origin, and gender were similar within pens. In each trial, eight pigs of each eating type were killed 5 days after weaning in order to determine villous heights and crypt depths in the proximal small intestine and volatile fatty acid (VFA) concentrations in the colon. While being suckled, body weight was not related to the pre-weaning consumption of creep food (P > 0.1) whereas average daily gain of the good eaters during the creep feeding period was higher (P <0.05) than that of the moderate and non-eaters. Both morphology measures and VFA concentrations on the day of weaning were unaffected (P > 0.1) by the pre-weaning food consumption. After weaning, food intake and gain of the total group of good eaters were higher (P <0.05) than that of the non-eaters, whereas villous height and villous height : crypt depth ratios did not differ (P > 0.1). Neither total VFA concentration nor the proportion of branched-chain VFA were affected by creep food consumption while being suckled. Total VFA concentration in the colon was positively associated with body-weight gain (P <0.001). This study confirms earlier findings that consumption of creep food while being suckled stimulates food intake and growth after weaning. However, the beneficial effects were not associated with a prevention of damage to morphology of the small intestine.
    Voorjaarsgebruik vanggewassen
    Laarhoven, G.C.P.M. van; Stienezen, M.W.J. ; Everts, H. ; Pol, A. van den - \ 2003
    Lelystad : Praktijkonderzoek Veehouderij (PraktijkRapport / Animal Sciences Group, Praktijkonderzoek : Rundvee ) - 21
    ondergewassen - lente - stikstof - maïskuilvoer - voedingswaarde - voer - rundveevoeding - catch crops - spring - nitrogen - maize silage - nutritive value - feeds - cattle feeding
    In this study important information was collected on cultivating catch crops and the subsequent crop of silage maize. Three catch crops and two different harvesting and ploughing-in dates were compared. Attention was paid to the yields of the catch crops and of the silage maize, the feed value, and the nitrogen efficiency. This report provides livestock farmers and intermediaires with more information to support their decisions about spring treatments of catch crops and their estimates of the cultivation risks.
    Optimale oogsttijdstip en conservering triticale-GPS = Optimal harvest date and conservation of whole crop triticale silage
    Kasper, G.J. ; Everts, H. - \ 2003
    Lelystad : Praktijkonderzoek Veehouderij (PraktijkRapport / Praktijkonderzoek Veehouderij : Rundvee ) - 36
    triticale - oogsttijdstip - kuilvoer van het hele gewas - conservering - nitraat - diervoeding - voer - kwaliteit - diervoedering - nederland - triticale - harvesting date - whole crop silage - conservation - nitrate - animal nutrition - feeds - quality - animal feeding - netherlands
    Triticale-geheleplantensilage (triticale-GPS) is een gewas dat in Nederland in opkomst is. Melkvee kan goed produceren op een rantsoen van graskuil en triticalekuil.
    Quantification of the effect of control strategies on classical swine fever epidemics
    Klinkenberg, D. ; Everts-van der Wind, A. ; Graat, E.A.M. ; Jong, M.C.M. de - \ 2003
    Mathematical Biosciences 186 (2003)2. - ISSN 0025-5564 - p. 145 - 173.
    subunit vaccine - marker vaccine - virus - transmission
    Emergency vaccination during an epidemic of classical swine fever virus (CSFV) has become a serious option because of the ethical problems of strategies with massive culling and the availability of a marker vaccine that reduces virus transmission. Here we present a model of between-herd CSFV transmission, which quantifies the effect of control strategies with and without vaccination. We estimate the model parameters from data of the Dutch CSFV epidemic of 1997/1998. With the model, a set of control strategies is compared, consisting of five control measures in several combinations. Consequently, the following general requirements of successful strategies can be formulated. First, to achieve extinction of a CSFV epidemic, transmission through transport should be prevented and the indirect virus transmission, i.e. all transmission not through animal contacts, should at least be halved, either by vaccination or by culling of the susceptible pig population. Second, to minimize the size and duration of an epidemic, the extinction requirements should be met quickly and indirect virus transmission should be reduced by far more than a half. Although the origin of the model parameters let the requirements in fact be only applicable for the south-eastern part of the Netherlands, it is argued that epidemics in other areas will not need stricter control strategies. (C) 2003 Elsevier Inc. All rights reserved.
    Hoogveenontwikkeling in veentjes en kleinschalige hoogveencomplexen op het Dwingelerveld; een landschapsbenadering. Deel 2 Landschapsontwikkeling en hydrologie
    Verschoor, A.J. ; Baaijens, G.J. ; Everts, F.H. ; Grootjans, A.P. ; Rooke, W. ; Schaaf, S. van der; Vries, N.P.J. de - \ 2003
    Ede : Expertisecentrum LNV (EC-LNV 2003/227-O) - 66 p.
    Individually assessed creep feed consumption by suckling piglets: influence on post-weaning feed intake characteristics and indicators of gut morphology
    Bruininx, E.M.A.M. ; Schellingerhout, A.B. ; Binnendijk, G.P. ; Peet-Schwering, C.M.C. van der; Schrama, J.W. ; Hartog, L.A. den; Everts, H. ; Beynen, A.C. - \ 2003
    In: Proceedings of the 9th International Symposium on Digestive Physiology in Pigs, University of Alberta, Banff Alberta, Canada - p. 143 - 145.
    Beperking van erosie bij de teelt van snijmais op veehouderijbedrijven
    Pol-van Dasselaar, A. van den; Laarhoven, G.C.P.M. van; Everts, H. - \ 2002
    Lelystad : Praktijkonderzoek Veehouderij (PraktijkRapport. Rundvee, paarden, schapen, geiten / Praktijkonderzoek Veehouderij 19) - 13
    zea mays - voedergewassen - bodemgeschiktheid - erosie - doorbuiging - drijfmest - nitraat - dierhouderij - nederland - limburg - zea mays - fodder crops - soil suitability - erosion - creep - slurries - nitrate - animal husbandry - netherlands - limburg
    In de periode 1999-2001 is in Zuid-Limburg onderzoek gedaan naar beperking van erosie bij de teelt van snijmaos na drijfmesttoediening in het voorjaar.
    Associations between individual feed intake characteristics and indicators of gut physiology of group housed weanling pigs differing in genotype.
    Bruininx, E.M.A.M. ; Schellingerhout, A.B. ; Lensen, E.G.C. ; Peet-Schwering, C.M.C. van der; Schrama, J.W. ; Everts, H. ; Hartog, L.A. den - \ 2002
    Animal Science 75 (2002)1. - ISSN 1357-7298 - p. 103 - 113.
    A prolonged photoperiod improves feed intake and energy metabolism of weanling pigs
    Bruininx, E.M.A.M. ; Heetkamp, M.J.W. ; Bogaart, D. van den; Peet-Schwering, C.M.C. van der; Beynen, A.C. ; Everts, H. ; Hartog, L.A. den; Schrama, J.W. - \ 2002
    Journal of Animal Science 80 (2002). - ISSN 0021-8812 - p. 1736 - 1745.
    Effect of creep feed consumption on individual feed intake characteristics andperformance of group-housed weanling pigs
    Bruininx, E.M.A.M. ; Binnendijk, G.P. ; Peet-Schwering, C.M.C. van der; Schrama, J.W. ; Hartog, L.A. den; Everts, H. ; Beynen, A.C. - \ 2002
    Journal of Animal Science 80 (2002). - ISSN 0021-8812 - p. 1413 - 1418.
    To assess the effects of creep feed consumption on individual feed intake characteristics and performance of group-housed weaned pigs, 16 litters (149 piglets) were fed a commercial creep feed (3,040 kcal NE/kg, 15.2 g lysine/kg) supplemented with 1% chromic oxide. Another five litters (48 piglets) were not given access to creep feed (no-feed). Piglets were weaned at 28 d after birth. On d 18, 22, and 27 of age, fecal samples from all the piglets were taken using fecal loops. A green color of the feces indicated that the piglet had eaten creep feed. Piglets that had green-colored feces three times were considered as eaters. Piglets that never showed green-colored feces were considered as non-eaters. At weaning 22 piglets of each type (no-feed, non-eaters, and eaters) were selected based on BW, litter origin, and sex. These 66 pigs were assigned to six pens equipped with computerized feeding stations. Eaters, non-eaters, and no-feed pigs were equally divided over all six pens. After weaning a prestarter (d 0 to 13) and a starter diet (d 14 to 34) were offered for ad libitum consumption. The individual feed intake characteristics of latency time (interval between weaning and first feed intake) and initial feed intake (intake during the first 24 h following first feed intake) and performance traits were determined for all piglets. The pigs that were designated as eaters needed less time between weaning and first feed intake than the pigs that were designated as non-eaters and no-feed pigs (P = 0.04 and P = 0.06, respectively). Initial feed intake was not affected (P > 0.1) by feed intake prior to weaning. However, during d 0 to 8 the eaters had more visits per day during which feed was consumed than both the non-eaters and no-feed pigs. Averaged over the first 8 d after weaning, the ADFI and ADG of the eaters were higher than that of the non-eaters and no-feed pigs (P < 0.05). Averaged over the total 34-d period the effect of creep feed intake on postweaning ADFI was much less pronounced (P = 0.20), whereas ADG of the eaters was the highest (P < 0.05). Creep feed intake during the sucking period stimulates early postweaning feed intake as well as postweaning performance.
    Rumen digesta kinetics in cold exposed prolific sheep: impact on protein evaluation
    Sebek, L.B.J. ; Everts, H. - \ 2002
    Animal Feed Science and Technology 101 (2002). - ISSN 0377-8401 - p. 17 - 29.
    Modelling control strategies during Classical Swine Fever epidemics
    Klinkenberg, D. ; Everts-van der Wind, A. ; Graat, E.A.M. ; Jong, M.C.M. de - \ 2002
    Mathematical Biosciences 2002 (2002). - ISSN 0025-5564 - p. xx - xx.
    The decrease in body fat in mice fed conjugated linoleic acid is due to increases in energy expenditure and energy loss in the excreta
    Terpstra, A.H.M. ; Beynen, A.C. ; Everts, H. ; Kocsis, S. ; Katan, M.B. ; Zock, P.L. - \ 2002
    The Journal of Nutrition 132 (2002)5. - ISSN 0022-3166 - p. 940 - 945.
    We carried out energy balance studies in four groups of young, growing, 5-wk-old Balb-C mice (n = 12/group) that were either food restricted or nonrestricted and fed high fat diets (38 energy%) with or without 0.93 g/100 g conjugated linoleic acid (CLA) for 39 d. The energy in carcasses, excreta and food was measured in a bomb calorimeter. CLA lowered the percentage of the energy intake that was stored in the body from 1.9 ± 0.8 to -2.3 ± 0.7% (mean ± SD, P < 0.05) in the nonrestricted mice and from 1.4 ± 1.3 to -2.9 ± 0.7% (P < 0.05) in the restricted mice. Thus, the CLA-treated mice had a net loss of body energy. The percentage of the energy intake eliminated in the excreta increased from 7.6 ± 0.9% in controls to 8.7 ± 1.0% (P < 0.05) in the CLA-treated mice that were nonrestricted and from 7.3 ± 0.8 to 8.4 ± 0.6 (P < 0.05) in the restricted mice. The amount of energy ingested minus the amount retained in carcasses and excreta equals the energy expenditure. The percentage of the energy intake that was expended as heat increased from 90.5 ± 1.2 in controls to 93.6 ± 1.5% (P < 0.05) in the CLA-treated nonrestricted mice and from 91.3 ± 1.5 to 94.5 ± 1.0% (P < 0.05) in the restricted mice. The lower energy storage in the CLA-fed mice was accounted for by an increase in the energy expenditure (74%) and by an increase in energy lost in the excreta (26%). Feeding CLA also increased liver weight, which may warrant further studies on the safety of CLA. -------------------------------------------------------------------------------- KEY WORDS: … mice … conjugated linoleic acid (CLA) … energy balance … energy expenditure
    Drijfmesttoediening op klei in voorjaar mogelijk
    Pol-van Dasselaar, A. van den; Everts, H. - \ 2001
    Rundvee praktijkonderzoek 14 (2001)1. - ISSN 1569-805X - p. 18 - 19.
    plantenvoeding - drijfmest - toepassing - opbrengen op het land - toepassingsdatum - toedieningswijzen - toedieningsapparatuur - mengmestverspreiders - rundveedrijfmest - maïs - klei - zware kleigronden - bodemverdichting - bodem - bodemstructuur - lente - veldproeven - bemesting - plant nutrition - slurries - application - application to land - application date - application methods - application equipment - slurry spreaders - cattle slurry - maize - clay - clay soils - soil compaction - soil - soil structure - spring - field tests - fertilizer application
    Voor de teelt van maos op kleigrond kan toediening van drijfmest uitgesteld worden tot het voorjaar. Voordelen hiervan zijn een betere benutting van de mineralen, minder mineralenverliezen en een lager stikstofoverschot.
    Aver Heino voldoet aan EU-richtlijn voor grondwater
    Pinxterhuis, I. ; Everts, H. - \ 2001
    Rundvee praktijkonderzoek 14 (2001)1. - ISSN 1569-805X - p. 6 - 7.
    nitraat - nitraten - plantenvoeding - stikstofmeststoffen - grondwater - grondwaterverontreiniging - uitspoelen - verliezen uit de bodem - verontreinigingsbeheersing - normen - eu regelingen - melkveehouderij - biologische landbouw - agrarische bedrijfsvoering - demonstratiebedrijven, landbouw - proefbedrijven - milieubescherming - conversie - bemesting - proefboerderijen - nitrate - nitrates - plant nutrition - nitrogen fertilizers - groundwater - groundwater pollution - leaching - losses from soil - pollution control - standards - eu regulations - dairy farming - organic farming - farm management - demonstration farms - pilot farms - environmental protection - conversion - fertilizer application - experimental farms
    Al in het eerste najaar van de omschakeling voldeden de meeste graslandpercelen aan de EU-richtlijn voor nitraat in het grondwater.
    Growth performance of weanling pigs fed spray-dried animal plasma: a review
    Dijk, A.J. van; Everts, H. ; Nabuurs, M.J.A. ; Margry, R. ; Beynen, A.C. - \ 2001
    Livestock Production Science 68 (2001). - ISSN 0301-6226 - p. 263 - 274.
    Spray-dried animal plasma (SDAP), mostly of porcine origin, is frequently used as an ingredient of weanling piglets diets in order to improve feed intake and to reduce post-weaning diarrhoea. On the basis of 15 published studies it is concluded that dietary SDAP levels up to 6% increase both average daily gain (ADG) and feed intake (ADFI) in the first 2 weeks after weaning in a dose-dependent fashion. Up to 6% SDAP also reduces feed conversion ratio (FCR). The positive effect of SDAP on ADG and ADFI is much more pronounced in the first than the second week after weaning. There is no positive carry-over effect of SDAP feeding during the period of 2 weeks after weaning on growth performance thereafter. SDAP is an expensive protein source and an economic evaluation should be made before including SDAP in weanling piglets diets. Multiple regression analysis indicated that, apart from SDAP dose, baseline growth rate is an important determinant of the effect of SDAP on ADG, with high baseline growth rate being associated with small effects of SDAP. It should be stressed that SDAP is a non-sterilised product that might spread certain diseases after feeding it to pigs. Porcine plasma has more beneficial effects than bovine plasma. Possible modes of action are discussed. It is suggested that, in addition to improving feed palatability, SDAP reduces post-weaning intestinal disease by preventing attachment of pathogens.
    Individually measured feed intake characteristics and growth performance of group-housed weanling pigs: effects of sex, initial body weight, and body weight distribution within groups
    Bruininx, E.M.A.M. ; Peet-Schwering, C.M.C. van der; Schrama, J.W. ; Vereijken, P.F.G. ; Vesseur, P.C. ; Everts, H. ; Hartog, L.A. den; Beynen, A.C. - \ 2001
    Journal of Animal Science 79 (2001)2. - ISSN 0021-8812 - p. 301 - 308.
    Feed intake characteristics of 192, 27-d-old weanling pigs housed in groups and given ad libitum access to feed and water were measured individually with the use of computerized feeding stations. The groups were either homogeneous or heterogeneous as to BW distribution; pigs of three defined initial BW classes were used (mean BW of 6.7, 7.9, or 9.3 kg). The effects of BW distribution, BW class, and sex were studied with regard to average performance traits, latency time (interval between weaning and first feed intake), initial feed intake (intake during the first 24 h following first feed intake), and daily increase in feed intake during the interval between first feed intake and the day on which energy intake met or exceeded 1.5 times the maintenance requirement. Homogeneous and heterogeneous groups had similar latency times, initial feed intakes, and daily increases in feed intake. For the period 0 to 34 d after weaning, ADFI and ADG were also similar for homogeneous and heterogeneous groups, but gain:feed ratio was greater (P < 0.05) in the homogeneous groups. Gilts had higher (P < 0.05) initial feed intakes than barrows and also had greater (P < 0.05) ADFI and ADG during the period 0 to 13 d after weaning. Pigs with average BW of 6.7 kg had higher (P < 0.05) initial feed intakes than their counterparts with average BW of 7.9 kg and 9.3 kg, but the daily increase in feed intake was similar for the three groups. The lighter pigs had more daily visits and a lower feed intake per visit and tended to have a shorter postweaning latency to the onset of feeding than the heavier pigs. This study indicates that the high variability in early feeding behavior among group-housed weanling pigs may be related to BW and sex.
    Protein metabolism of prolific ewes during late gestation and early lactation
    Sebek, L.B.J. - \ 2001
    Wageningen University. Promotor(en): S. Tamminga; H. Everts. - S.l. : S.n. - ISBN 9789058085092 - 132
    schapen - ooien - diervoedering - sheep - ewes - animal feeding

    Subject headings: protein / metabolism / ewes

    Introduction of prolific crossbred ewes and a new protein evaluation system for ruminants, the DVE/OEB system, necessitate a reconsideration of ewe feeding strategies. The objective of this thesis is to investigate the amount of protein digested in the small intestine (DVE), the efficiency by which the digested protein is used during late pregnancy and early lactation and to evaluate current Dutch protein feeding strategy. This feeding strategy was developed for ewes bearing one or two lambs, but it shows that prolific ewes yield approximately10% more DVE from the same feed. This is due to rumen degradation enhancement resulting in larger amounts of effectively degraded protein and synthesised microbial protein in prolific ewes. Winter shearing results in further increases in DVE yield. Current DVE maintenance requirements and production efficiencies are also valid for prolific ewes. The DVE requirements for prolific ewes, calculated from the measured protein production and efficiency of protein utilisation, are in agreement with the requirements derived from the feeding trials during late pregnancy and early lactation. Adaptation of the current protein feeding strategy is proposed for prolific crossbred ewes.

    The IVOG feeding station: a tool for monitoring the individual feed intake of group-housed weanling pigs
    Bruininx, E.M.A.M. ; Peet-Schwering, C.M.C. van der; Schrama, J.W. ; Vesseur, P.C. ; Everts, H. ; Beynen, A.C. - \ 2001
    Journal of Animal Physiology and Animal Nutrition 85 (2001). - ISSN 0931-2439 - p. 81 - 87.
    Three batches of weanling pigs (total n=310 pigs) were used in a 34-day experiment to validate the use of an IVOG? feeding station as a tool for monitoring individual feed intake of group-housed weanling pigs. An IVOG? feeding station for weanling pigs consists of a single-space dry feeder placed on a load cell in combination with electronic identification. Data of 192 weanling pigs (18 pens) fed by IVOG? feeding stations were used to develop a protocol for the screening of IVOG? data. To assess the quality of the IVOG? data, the feed intake per pen computed from the screened IVOG? data was compared with the feed intake calculated from feed weighing. To assess the suitability of the use of IVOG? feeding stations under practical pig husbandry conditions, performance of 96 weanling pigs fed by the IVOG? feeding stations was compared with that of 118 weanling pigs that were fed using commercial single-space dry feeders (11 pens). Feed intake per pen computed from the IVOG? data was similar to the feed intake calculated from feed weighing (average recovery 101.1€for all test periods (p > 0.1). Furthermore, feed recovery did not differ among feeding stations (p > 0.1). During the first 13 days after weaning, the average daily feed intake (ADFI) of weanling pigs fed by the single-space dry feeders was higher (p < 0.05) than that of weanling pigs fed by the feeding stations. Average daily gain and gain to feed ratios did not differ (p > 0.1) between both feeding systems. During the remaining 21 days and averaged over the entire experimental period, performance did not differ between the feeding systems (p > 0.1). It can be concluded that IVOG? feeding stations for weanling pigs are a suitable tool to monitor individual feed intake of group-housed weanling pigs.
    A model to evaluate control measures against the spread of classical swine fever virus
    Klinkenberg, D. ; Everts-van der Wind, A. ; Jong, M.C.M. de - \ 2001
    In: Proceedings of the 14th annual meeting of the VEEC, Wageningen, 2001 / E.A.M. Graat, K. Frankena. - Wageningen : Wageningen University, 2001. - ISBN 90-6754-656-9 - p. 59 - 68.
    Teelt van mais op klei-op-veengrond. Bemesting en grondbewerking
    Pol-van Dasselaar, A. van den; Houwelingen, K.M. van; Everts, H. - \ 2000
    Lelystad : Praktijkonderzoek Rundvee, Schapen en Paarden (Rapport / Praktijkonderzoek Rundvee, Schapen en Paarden 185) - 23
    maïs - kunstmeststoffen - bevruchting - stikstof - fosfaat - toedieningshoeveelheden - grondbewerking - zaaibedbereiding - zware kleigronden - veengronden - uitspoelen - nederland - zuid-holland - bemesting - maize - fertilizers - fertilization - nitrogen - phosphate - application rates - tillage - seedbed preparation - clay soils - peat soils - leaching - netherlands - zuid-holland - fertilizer application
    rapport bevat de resultaten van veldproeven op het gebied van bemesting en grondbewerking, die in de periode 1997-1999 door proefbedrijf Zegveld van het Praktijkonderzoek Rundvee, Schapen en Paarden (PR) zijn uitgevoerd.
    Mineralenbalans en grondwaterkwaliteit bij deelnemers Bioveem
    Snijders, P. ; Everts, H. - \ 2000
    Praktijkonderzoek Rundvee, Schapen en Paarden. Praktijkonderzoek 13 (2000)6. - ISSN 1386-8470 - p. 31 - 33.
    melkveehouderij - biologische landbouw - zandgronden - mineralen - boekhouding - grondwater - chemische eigenschappen - nitraten - dairy farming - organic farming - sandy soils - minerals - accounting - groundwater - chemical properties - nitrates
    Het nitraatgehalte in het grondwater voldoet aan de EU norm van 50 mg per l. Ook het stikstof en fosfaat overschot op de mineralenbalans voldoet op de meeste bedrijven.
    Directzaai van mais biedt perspectief
    Pol-van Dasselaar, A. van den; Everts, H. - \ 1999
    Praktijkonderzoek Rundvee, Schapen en Paarden. Praktijkonderzoek 12 (1999)6. - ISSN 1386-8470 - p. 22 - 24.
    maïs - erosiegevoeligheid - precisiezaaimachines - teelt - cultuurmethoden zonder grondbewerking - zware kleigronden - veengronden - löss - krimp - zaaien - maize - erodibility - precision drills - cultivation - no-tillage - clay soils - peat soils - loess - shrinkage - sowing
    Maos wordt meestal met een precisiezaaimachine in een goed bewerkt zaaibed gezaaid. Het is echter ook mogelijk om maos zonder een grondbewerking direct in gras of een wintergewas te zaaien. Hiermee wordt bespaard op de grondbewerkingskosten, maar het brengt wel extra teeltrisico met zich mee. In Nederland is directzaai voor bepaalde gebieden mogelijk interessant.
    Maisteelt op klei-op-veengrond vraagt extra aandacht
    Pol-van Dasselaar, A. van den; Houwelingen, K. van; Everts, H. - \ 1999
    Praktijkonderzoek Rundvee, Schapen en Paarden. Praktijkonderzoek 12 (1999)2. - ISSN 1386-8470 - p. 3 - 5.
    maïs - zea mays - plantenvoeding - stikstofmeststoffen - droge stof - opbrengsten - zware kleigronden - veengronden - maize - zea mays - plant nutrition - nitrogen fertilizers - dry matter - yields - clay soils - peat soils
    De teelt van mais op klei-op-veengrond is aantrekkelijk, mits aan een aantal voorwaarden wordt voldaan: goede ontwatering, kleidek van minimaal 25 cm en aangepaste grondbewerking ter voorkoming van maaivelddaling.
    Quantification of absorbability and requirements of macroelements
    Jongbloed, A.W. ; Everts, H. ; Kemme, P.A. ; Mroz, Z. ; Kyriazakis, I. - \ 1999
    In: A quantitative biology of the pig - p. 275 - 298.
    Beregening op Maat 1997 : toetsing van de beregeningsplanner en - wijzer in de praktijk : onderzoeksresultaten 1997 = Customised sprinkle irrigation 1997 : trialling of the irrigation planner and irrigation guide
    Hoving, I.E. ; Everts, H. ; Alblas, J. - \ 1998
    Lelystad : Praktijkonderzoek Rundvee, Schapen en Paarden (Rapport / Praktijkonderzoek Rundvee, Schapen en Paarden 172) - 115
    beregening met sproeiers - water - watervoorziening - landbouw - bodemwaterbalans - nederland - noord-brabant - sprinkler irrigation - water - water supply - agriculture - soil water balance - netherlands - noord-brabant
    Voedergewassen op droogtegevoelige gronden zonder beregening
    Stienezen, M. ; Everts, H. ; Snijders, P. ; Schans, D. van der - \ 1998
    Praktijkonderzoek Rundvee, Schapen en Paarden. Praktijkonderzoek 11 (1998)3. - ISSN 1386-8470 - p. 28 - 31.
    droge stof - zea mays - maïs - triticale - medicago - beta vulgaris - suikerbieten - zandgronden - voedergrassen - dry matter - zea mays - maize - triticale - medicago - beta vulgaris - sugarbeet - sandy soils - fodder grasses
    Een te grote onttrekking van grondwater is de oorzaak dat het grondwaterpeil in sommige gebieden de laatste decennia aanzienlijk is gedaald. De landbouw neemt ongeveer eenderde van de grondwateronttrekking voor haar rekening. Het beleid zoekt naar mogelijkheden om het gebruik van grondwater door de landbouw te beperken
    Calcium, phosphorus and magnesium retention and excretion in pregnant sows during three parities
    Everts, H. ; Jongbloed, A.W. ; Dekker, R.A. - \ 1998
    Livestock Production Science 53 (1998). - ISSN 0301-6226 - p. 113 - 121.
    Calcium, magnesium and phosphorus balance of sows during lactation for three parities
    Everts, H. ; Jongbloed, A.W. ; Dekker, R.A. - \ 1998
    Livestock Production Science Submitted (1998). - ISSN 0301-6226
    Absorbability and requirements of macroelements for pigs
    Jongbloed, A.W. ; Everts, H. ; Kemme, P.A. ; Mroz, M. - \ 1998
    In: Toward a quantitative biology of the pig / Kyriazakis, I., - p. 275 - 298.
    Voeding in relatie tot gezondheid (zeugen).
    Kemp, B. ; Everts, H. ; Hartog, L.A. den - \ 1998
    In: Preventieve gezondheidszorg in de varkenshouderij / den Hartog, L.A., Visser-Reyneveld, M.I., Wageningen : PHLO - p. 57 - 68.
    Beregeningswijzer
    Everts, H. ; Hoving, I.E. ; Schans, D.A. van der - \ 1997
    Lelystad : Praktijkonderzoek Rundvee, Schapen en Paarden (Themaboek 21) - 10 p.
    Het PV heeft een beregeningswijzer in de vorm van een parkeerschijf ontwikkeld. Deze wordt dit jaar samen met de beregeningsplanner van het CLM in het project Beregenen op Maat in Noord-Brabant getest. Na bepaling van de vochtvoorraad van de grond, kan door draaien aan de schijf eenvoudig de beregeningsgift worden afgelezen.
    Beregenen op maat : toetsing van de beregeningsplanner in de praktijk : onderzoeksresultaten 1996
    Hoving, I.E. ; Everts, H. ; Schans, D.A. van der - \ 1997
    Lelystad : Praktijkonderzoek Rundvee, Schapen en Paarden (Rapport / Praktijkonderzoek Rundvee, Schapen en Paarden 165) - 132
    beregening met sproeiers - water - watervoorziening - landbouw - bodemwaterbalans - nederland - noord-brabant - sprinkler irrigation - water - water supply - agriculture - soil water balance - netherlands - noord-brabant
    Let op bandenspanning bij berijden grasland!
    Wouters, B. ; Everts, H. ; Dijkstra, B. - \ 1997
    Praktijkonderzoek Rundvee, Schapen en Paarden. Praktijkonderzoek 10 (1997)1. - ISSN 1386-8470 - p. 10 - 11.
    karren - deformatie - droge stof - graslanden - veengronden - bodem - bodemverdichting - bodemstructuur - trailers - banden - belastingskenmerken (grond) - machines - carts - deformation - dry matter - grasslands - peat soils - soil - soil compaction - soil structure - trailers - tyres - bearing characteristics - machines
    Het verlagen van de bandspanning vergroot de mogelijkheden voor het berijden zoals bleek uit onderzoek op veengrasland. Ook het berijden van grasland op zware klei bij sub-optimale omstandigheden blijkt nadelig voor de grasopbrengst.
    De protale nutriëntenflux bij groeiende vleesvarkens gevoerd met rantsoenen met een hoog aandeel natief tapioca- of verstijfseld tarwezetmeel
    Bakker, J.G.M. ; Meulen, J. van der; Dekker, R.A. ; Everts, H. ; Smits, B. ; Visser, H. de - \ 1997
    Unknown Publisher (Rapport ID-DLO )
    Effect of resistant starch on net portal-drained viscera flux of glucose, volatile fatty acids, urea, and ammonia in growing pigs
    Meulen, J. van der; Bakker, G.C.M. ; Bakker, J.G.M. ; Visser, H. de; Jongbloed, A.W. ; Everts, H. - \ 1997
    Journal of Animal Science 75 (1997)10. - ISSN 0021-8812 - p. 2697 - 2704.
    Net portal-drained viscera (PDV) flux of glucose, VFA, ammonia, and urea was determined in pigs fed diets with or without resistant starch. Diets consisted of 65% cornstarch (diet CS), 32.5% cornstarch and 32.5% raw potato starch (diet CPS), or 65% raw potato starch (diet PS); the remaining 35% supplied all amino acids, fat, fiber, minerals, and vitamins. The diets contained twice the maintenance requirement for energy and were fed twice daily to four barrows (initial BW 56 kg) in three periods in a crossover design. The pigs were fitted with catheters in a mesenteric vein, a mesenteric-artery, and the portal vein, and net PDV flux was calculated by multiplying portal-arterial concentration differences and corresponding portal vein flow. Net PDV flux of glucose was significantly less after feeding diets CPS and PS, and portal absorption of ileally digested glucose was 89, 66, and 41% for diets CS, CPS, and PS, respectively. Net PDV flux of VFA was lowest after feeding diet CS and three to four times higher after feeding diets CPS and PS. Net PDV flux of ammonia was highest for diet CS and almost halved after feeding diets CPS and PS. There was a small negative net PDV flux of urea for diets CS and CPS, which significantly increased after feeding diet PS. These results suggest that excretion of nitrogen is shifted from urine to feces primarily by reduction of the net PDV flux of ammonia when resistant starch is fed.
    Wat doet wisselbouw van maïs en gras?
    Dijk, W. van; Baan Hofman, T. ; Everts, H. - \ 1997
    PAV-bulletin. Akkerbouw (1997). - p. 15 - 16.
    Water depletion, an important cause of mortality in females of the spider Oedothorax apicatus after treatment with Deltamethrin; a simulation study.
    Jagers op Akkerhuis, G.A.J.M. ; Rossing, W.A.H. ; Piet, G. ; Everts, J.W. - \ 1997
    Pesticide Biochemistry and Physiology 58 (1997). - ISSN 0048-3575 - p. 1 - 76.
    Grote variatie in opbrengsten voedergewassen op droogtegevoelige grond
    Schans, D. van der; Everts, H. ; Snijders, P. ; Aarts, F. - \ 1996
    Praktijkonderzoek Rundvee, Schapen en Paarden. Praktijkonderzoek 9 (1996)3. - ISSN 1386-8470 - p. 16 - 18.
    voedergewassen - zandgronden - vloeistoffen (liquids) - omloop - absorptie - emissie - gewassen - waterbehoefte - water - vochtigheid - droogte - droge stof - fodder crops - sandy soils - liquids - circulation - absorption - emission - crops - water requirements - water - humidity - drought - dry matter
    Ook bleken tussen de gewassen grote verschillen in efficiëntie waarmee het beschikbare water wordt gebruikt voor productie.
    Skin and liver diseases in flounder (Platichthys flesus) after long-term exposure to contaminated sediments in large-scale mesocosms
    Vethaak, A.D. ; Jol, J.G. ; Meijboom, A. ; Eggens, M.L. ; Rheinallt, T. ; Wester, P.W. ; Zande, T. van de; Bergman, A. ; Dankers, N. ; Ariese, F. ; Baan, R.A. ; Everts, J.M. ; Opperhuizen, A. ; Marquenie, J. - \ 1996
    Environmental Health Perspectives 104 (1996)11. - ISSN 0091-6765 - p. 1218 - 1229.
    Effecten van mais-gras vruchtwisseling
    Dijk, W. van; Baan Hofman, T. ; Nijssen, K. ; Everts, H. ; Wouters, A.P. ; Lamers, J.G. ; Alblas, J. ; Bezooijen, J. van - \ 1996
    Unknown Publisher - 140 p.
    Nutritional aspects of the lactating sow.
    Kemp, B. ; Hartog, L.A. den; Everts, H. - \ 1996
    In: Abstracts 46th Ann. Meeting of the EAAP / van Arendonk, J.A.M., Wageningen Pers - p. 260 - 260.
    Mengteelt van populieren met suikerbieten, snijmaos en gras. Deel 2 en 3: Groei en opbrengst van bomen en landbouwgewassen in mengteelt en verzamelde data van mengteeltonderzoek
    Bervaes, J.C.A.M. ; Dik, E.J. ; Edelenbosch, N.H. ; Everts, H. ; Schans, D.A. van der; Westerdijk, C.E. - \ 1995
    Wageningen [etc.] : IBN-DLO [etc.]
    bosbouw - meervoudig landgebruik - gemengde teelt - agroforestry - tussenteelt - meervoudige teelt - tussenplanting - bomen - graslanden - beta vulgaris - suikerbieten - zea mays - maïs - kosten-batenanalyse - nederland - forestry - multiple land use - mixed cropping - agroforestry - intercropping - multiple cropping - interplanting - trees - grasslands - beta vulgaris - sugarbeet - zea mays - maize - cost benefit analysis - netherlands
    Polybrominated biphenyl and diphenylether flame retardants: analysis, toxicity and environmental occurence
    Pijnenburg, A.M.C.M. ; Everts, J.W. ; Boer, J. de; Boon, I.P. - \ 1995
    Reviews of Environmental Contamination and Toxicology 141 (1995). - ISSN 0179-5953 - p. 1 - 26.
    Effect of protein supply during pregnancy and lactation on body composition of sows during three reproductive cycles
    Everts, H. ; Dekker, R.A. - \ 1995
    Livestock Production Science 43 (1995). - ISSN 0301-6226 - p. 137 - 147.
    Effect of protein supply during pregnancy on body composition of gilts and their products of conception
    Everts, H. ; Dekker, R.A. - \ 1995
    Livestock Production Science 43 (1995). - ISSN 0301-6226 - p. 27 - 36.
    Effecten van vruchtwisseling en bodembiociden op de opbrengst van gras
    Baan Hofman, T. ; Wouters, B. ; Everts, H. ; Bezooijen, J. van - \ 1995
    In: Gebundelde Verslagen van de Nederlandse Vereniging voor weide- en Voederbouw - p. 28 - 32.
    Normen voor lacterende zeugen.
    Everts, H. ; Blok, M.C. ; Kemp, B. ; Peet-Schwering, C.M.C. van der; Smits, C.H.M. - \ 1995
    Lelystad : CVB - 45
    diervoedering - verteerbaarheid - energetische waarde - voedingsrantsoenen - voer - handboeken - lactatie - zeugen - zogen - animal feeding - digestibility - energy value - feed rations - feeds - handbooks - lactation - sows - suckling
    Effect bodemverdichting op opbrengst en stikstofopname Engels raaigras op zandgrond = Effect of soil compaction on yield and nitrogen uptake of English ryegrass on a sandy soil
    Snijders, P.J.M. ; Wopereis, F.A. ; Everts, H. ; Wouters, A.P. - \ 1994
    Lelystad : Proefstation voor de Rundveehouderij, Schapenhouderij enPaardenhouderij (Rapport / Proefstation voor de Rundveehouderij, Schapenhouderij en Paardenhouderij P.R. 152) - 51
    graslanden - stikstof - plantenontwikkeling - plantenfysiologie - wortels - zandgronden - bodemverdichting - oogsttoename - oogstverliezen - opbrengsten - machines - grasslands - nitrogen - plant development - plant physiology - roots - sandy soils - soil compaction - yield increases - yield losses - yields - machines
    Populierenteelt in combinatie met gras mogelijk
    Everts, H. - \ 1994
    Praktijkonderzoek / Praktijkonderzoek Rundvee, Schapen en Paarden (PR), Waiboerhoeve 7 (1994)2. - ISSN 0921-8874 - p. 42 - 44.
    Ook is het mogelijk de vrijgekomen grond te gebruiken voor andere teelten. In dit artikel staat de populierenteelt centraal.
    Normen voor dragende zeugen.
    Everts, H. ; Blok, M.C. ; Kemp, B. ; Peet-Schwering, C.M.C. van der; Smits, C.H.M. - \ 1994
    In: CVB-documentatierapport no. 9, Centraal Veevoeder Bureau, Lelystad, Nederland - p. 51 - 51.
    verteerbaarheid - energetische waarde - voedingsrantsoenen - voer - handboeken - zeugen - digestibility - energy value - feed rations - feeds - handbooks - sows
    Nitrogen and energy metabolism of sows during several reproductive cycles in relation to nitrogen intake
    Everts, H. - \ 1994
    Agricultural University. Promotor(en): M.W.A. Verstegen; A.W. Jongbloed. - S.l. : Everts - 156
    afmesten - zeugen - metabolisme - voer - eiwitten - finishing - sows - metabolism - feeds - proteins

    By feeding the same diet during pregnancy and lactation sows are fed above the nitrogen requirement during pregnancy due to the relatively high nitrogen requirement during lactation. For feeding closer to the requirements at least two diets are needed: one diet with a low nitrogen content during pregnancy and an other diet with a higher nitrogen level during lactation. The consequences of such a feeding strategy were investigated. A lower nitrogen supply during pregnancy had no effect on the development of the products of conception, but the development of the maternal protein mass was reduced during the first reproductive cycle. In the second and third parity a compensatory effect was observed. The nitrogen excretion decreased with about 35 % during pregnancy. Sow with a higher lysine supply during lactation mobilized a less nitrogen and more fat during lactation. The nitrogen excretion per sow per year can be reduced with about 25 %. However, the efficiency of the utilization of dietary nitrogen into retained nitrogen remained at a relatively low level.

    For an further improvement of the efficiency of the utilization of dietary nitrogen into retained nitrogen a multiphase feeding strategy in combination with a restricted development of the live weight of sows is proposed.

    Een vergelijking tussen voordrooggrassilage en grashooi als ruwvoer voor meerling dragende ooien = A comparison between grass-silage and grass-hay as a roughage for ewes pregnant of a large litter
    Sebek, L.B.J. ; Everts, H. - \ 1993
    Lelystad : IVVO-DLO (Rapport / IVVO-DLO no. 246) - 30
    voer - vrouwelijke dieren - hooi - voedingswaarde - kuilvoer - feeds - female animals - hay - nutritive value - silage
    Het schapenonderzoek in de komende jaren
    Everts, H. - \ 1993
    Lelystad : IVVO-DLO (Mededelingen / Instituut voor Veevoedings Onderzoek no. 23) - 17
    dierhouderij - nederland - onderzoek - schapen - animal husbandry - netherlands - research - sheep
    Fosfaat in bodem en gewas bij wel en niet injecteren
    Middelkoop, J.C. van; Boer, D.J. den; Wouters, A.P. ; Everts, H. - \ 1993
    Praktijkonderzoek / Praktijkonderzoek Rundvee, Schapen en Paarden (PR), Waiboerhoeve 6 (1993)3. - ISSN 0921-8874 - p. 14 - 17.
    toedieningswijzen - droge stof - efficiëntie - stalmest - kunstmeststoffen - graslanden - fosfor - application methods - dry matter - efficiency - farmyard manure - fertilizers - grasslands - phosphorus
    Uit onderzoek in de jaren 1984 t/m 1987 naar de fosfaatwerking bij éénmalig injecteren van dunne rundermest is gebleken dat fosfaat uit de mest pas na de eerste snede tot werking kwam.
    Apparent digestible phosphorus in the feeding of pigs in relation to availability, requirement and environment. 2. The requirement of digestible phosphorus for piglets, growing-finishing pigs and breeding sows.
    Jongbloed, A.W. ; Everts, H. - \ 1992
    Netherlands Journal of Agricultural Science 40 (1992)2. - ISSN 0028-2928 - p. 123 - 136.
    Twee-fasen-voedering in vergelijking met een-fase-voedering bij zeugen
    Everts, H. ; Sebek, L.B.J. ; Hoofs, A. - \ 1992
    Praktijkonderzoek varkenshouderij 6 (1992)1. - ISSN 1382-0346 - p. 21 - 23.
    Gedurende de dracht is de behoefte aan stikstof (N) en fosfor (P) ten opzichte van de energiebehoefte geringer dan tijdens de lactatie. Bij gebruik van één voersoort voor dracht en lactatie zijn de gehalten aan N en P in het voer afgestemd op de hogere behoefte tijdens de zoogperiode. Dat betekent een overmaat aan N en P tijdens de dracht.
    Invloed emissiearm mesttoedienen op graszode
    Everts, H. ; Geurink, J.H. ; Romme, A. - \ 1992
    Praktijkonderzoek / Praktijkonderzoek Rundvee, Schapen en Paarden (PR), Waiboerhoeve 5 (1992)2. - ISSN 0921-8874 - p. 10 - 12.
    luchtverontreiniging - ammoniak - emissie - kunstmeststoffen - graslanden - injectie - mest - plantenvoeding - bodeminjecteurs - vervluchtiging - air pollution - ammonia - emission - fertilizers - grasslands - injection - manures - plant nutrition - soil injectors - volatilization
    Het onderzoek richtte zich op de technische uitvoering van het werk en de effecten op de graszode bijvoorbeeld verdroging en zodekwaliteit.
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