Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Biological treatment of gas pollutants in partitioning bioreactors
    Lebrero, Raquel ; Frutos Osvaldo, David ; Pérez, Victor ; Cantera, Sara ; Estrada, José Manuel ; Muñoz, Raúl - \ 2019
    In: Advances in Chemical Engineering / Huerta-Ochoa, Sergio, Castillo-Araiza, Carlos O., Quijano, Guillermo, Academic Press Inc. (Advances in Chemical Engineering ) - ISBN 9780128149966 - p. 239 - 274.
    Gas treatment - Gas-liquid mass transfer - Hydrophobic pollutants - NAP - Robustness

    The off-gas treatment of hydrophobic pollutants in conventional biotechnologies is typically limited by the gas-liquid mass transfer as a result of the low-concentration gradient imposed by their high partitioning coefficients. The addition of a non-aqueous-phase (NAP) with a high affinity for the gas pollutant to packed bed or suspended-growth bioreactor configurations has resulted in enhanced elimination capacities and process robustness. Despite recent research has just added more desired characteristics to the selection criteria of the optimum NAP in gas treatment applications, silicone oil continues being the most popular mass transfer vector. This technology has been successfully applied to the treatment of hexane, methane, styrene, alpha-pinene and BTEX in biofilters, biotrickling filters, stirred tank and airlift bioreactors. The maximum potential of two-phase partitioning bioreactors (TPPBs) during off-gas treatment is achieved when the microbial community is confined inside the NAP. TPPB modeling has also experienced significant advances, the most advanced platforms being able to describe the recent findings in the field. This chapter will compile and critically discuss the fundamentals and most recent breakthroughs in the field of TPPBs for gas treatment applications.

    Scientific opinion on the proposed amendment of the EU specifications for titanium dioxide (E 171) with respect to the inclusion of additional parameters related to its particle size distribution
    Younes, Maged ; Aquilina, Gabriele ; Castle, Laurence ; Engel, Karl Heinz ; Fowler, Paul ; Frutos Fernandez, Maria Jose ; Gürtler, Rainer ; Gundert-Remy, Ursula ; Husøy, Trine ; Mennes, Wim ; Agneta Oskarsson, Peter Moldeus ; Rainieri, Sandra ; Shah, Romina ; Waalkens-Berendsen, Ine ; Wölfle, Detlef ; Gaffet, Eric ; Mast, Jan ; Peters, Ruud ; Rincon, Ana Maria ; Fürst, Peter - \ 2019
    EFSA Journal 17 (2019)7. - ISSN 1831-4732
    E 171 - food additive - particle size - specifications - Titanium dioxide

    The present opinion deals with the assessment of the data provided by interested business operators in support of an amendment of the EU specifications for titanium dioxide (E 171) with respect to the inclusion of additional parameters related to its particle size distribution. Titanium dioxide which is used as a food additive E 171 in food undergoes no surface treatment and is not coated. It consists of anatase or rutile generally containing small amounts of the other phase (rutile or anatase, < 2% m/m) and it may also contain small quantities (< 0.5%) of constituent particle growth and crystal phase control agents (alumina, sodium or potassium in combination with phosphate). Particle size analyses, by TEM, SEM, XDC or DC, have been carried out on five commercial brands of anatase E 171 and one of rutile E 171 manufactured by the only three EU manufacturers that, according to information submitted by interested business operators, produce food-grade titanium dioxide. Interested business operators proposed to introduce in the EU specifications for E 171 a specification of more than 100 nm for median Feret min diameter and less than 50% of the number of constituent particles below 100 nm; measured by EM in both cases. The Panel, after reviewing the data, concluded that a specification of more than 100 nm for median minimal external dimension, equivalent to less than 50% of the number of constituent particles with a median minimal external dimension below 100 nm, should be inserted in the current EU specifications. The Panel considered that the conclusions made, and the uncertainties identified, in the previous EFSA assessments on E 171 remain valid. The Panel reiterates the need for the further research as recommended in the previous opinions in order to decrease the level of uncertainty and acknowledged that additional studies with characterised E 171 are being carried out by interested business operators.

    Technologies for the bio-conversion of GHGs into high added value products : Current state and future prospects
    Cantera, Sara ; Frutos, Osvaldo D. ; López, Juan Carlos ; Lebrero, Raquel ; Torre, Raúl Muñoz - \ 2017
    In: Carbon Footprint and the Industrial Life Cycle / Alvarez Fernandez, Roberto, Zubelzu, Sergio, Martinez, Rodrigo, Springer Verlag (Green Energy and Technology 9783319549835) - ISBN 9783319549835 - p. 359 - 388.

    Today, methane (CH4) and nitrous oxide (N2O) emissions represent 20% of the total greenhouse gas (GHG) inventory worldwide. CH4 is the second most important GHG emitted nowadays based on both its global warming potential (25 times higher than that of CO2) and its emission rates, while N2O is the main O3-depleting substance emitted in this 21st century. However, despite their environmental relevance and the forthcoming stricter legislation on atmospheric GHG emissions, the development of cost-efficient and environmentally friendly GHG treatment technologies is still limited. In this context, an active bio-technological abatement of CH4 and N2O emissions combined with the production of high added value products can become a profitable alternative to mitigate GHGs emissions. The feasible revalorization of diluted CH4 emissions from landfills has been recently tested in bioreactors with the production of ectoine, a microbial molecule with a high retail value in the cosmetic industry (approximately $1300 kg−1), as well as with the generation of polyhydroxyalkanoates (PHAs), a commodity with potential to replace conventional petroleum-derived polymers. This CH4 bio-refinery approach can be also based on the biogas produced from anaerobic digestion, therefore improving the economic viability of this waste management technology. The N2O contained in emissions from nitric acid production processes can be also considered as a potential substrate for the production of PHAs, with the subsequent increase in the cost-effectiveness of the abatement strategies of this GHG. On the other hand, the off-gas N2O abatement from diluted wastewater treatment plant emissions has been recently confirmed, although at the expense of a high input of electron donor due to the need to first deplete the O2 transferred from the emission. This chapter constitutes a critical review of the state-of-the-art of the potential and research niches of bio-technologies applied in a CH4 and N2O bio-refinery approach.

    Nitrous Oxide Abatement Coupled with Biopolymer Production As a Model GHG Biorefinery for Cost-Effective Climate Change Mitigation
    Frutos, Osvaldo D. ; Cortes, Irene ; Cantera, Sara ; Arnaiz, Esther ; Lebrero, Raquel ; Muñoz, Raúl - \ 2017
    Environmental Science and Technology 51 (2017)11. - ISSN 0013-936X - p. 6319 - 6325.

    N2O represents ∼6% of the global greenhouse gas emission inventory and the most important O3-depleting substance emitted in this 21st century. Despite its environmental relevance, little attention has been given to cost-effective and environmentally friendly N2O abatement methods. Here we examined, the potential of a bubble column (BCR) and an internal loop airlift (ALR) bioreactors of 2.3 L for the abatement of N2O from a nitric acid plant emission. The process was based on the biological reduction of N2O by Paracoccus denitrificans using methanol as a carbon/electron source. Two nitrogen limiting strategies were also tested for the coproduction of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) coupled with N2O reduction. High N2O removal efficiencies (REs) (≈87%) together with a low PHBV cell accumulation were observed in both bioreactors in excess of nitrogen. However, PHBV contents of 38-64% were recorded under N limiting conditions along with N2O-REs of ≈57% and ≈84% in the ALR and BCR, respectively. Fluorescence in situ hybridization analyses showed that P. denitrificans was dominant (>50%) after 6 months of experimentation. The successful abatement of N2O concomitant with PHBV accumulation confirmed the potential of integrating biorefinery concepts into biological gas treatment for a cost-effective GHG mitigation.

    Modifications in liver transcriptomic profile of fattening lambs by early suckled milk intake level
    Santos, A. ; Giraldez, Francisco Javier ; Groenen, M. ; Madsen, O. ; Frutos, Javier ; Valdes, Carmen ; Andres, S. - \ 2017
    - p. 388 - 388.
    The increment of world population increases the need of improving feed efficiency traits of livestock. However, the molecular mechanisms behind different feed efficiency traits and their regulation by nutrition remain poorly understood. Nowadays, Next Generation Sequencing methods allow understanding differences in gene expression and identifying functional candidate genes and pathways that control target traits in order to design strategies to increase feed efficiency. The present study was designed to identify (RNA-seq) differentially expressed (DE) genes in the liver tissues of fattening merino lambs caused by milk restriction during the suckling period. Forty male lambs were assigned randomly to two intake levels (n=20 per group) during the suckling period, namely ad libitum (ADL) and restricted (RES) groups. When they reached 15 kg of live body weight (LBW), all the animals were offered the same complete pelleted diet at a restricted level (40 g/kg) to ensure no selection of ingredients and no differences in dry matter intake during the fattening period. All the lambs were slaughtered with 27 kg of LBW and four animals from each group were selected for RNA-seq. Thirty-eight DE annotated genes were identified, with 23 DE genes being down-regulated and 15 up-regulated in the RES relative to the ADL group. RES lambs showed over-expression of lipid and xenobiotic metabolism pathways. Moreover, those genes involved in protein synthesis or protease inhibitors were down-regulated in the RES group, whereas those related to proteolytic degradation were up-regulated, thus suggesting a higher catabolism of proteins in these lambs. In conclusion, a restricted milk intake level during the suckling period of merino lambs promoted long term effects on hepatic transcriptomic profile which might have modified fatty acids metabolism and increased catabolism of proteins and detoxification of xenobiotics during the fattening period.
    Identification of loci affecting accumulation of secondary metabolites in tomato fruit of a Solanum lycopersicum × Solanum chmielewskii introgression line population
    Ballester Frutos, A.R. ; Tikunov, Yury ; Molthoff, Jos ; Grandillo, Silvana ; Viquez-Zamora, Marcela ; Vos, Ric de; Maagd, Ruud A. de; Heusden, Sjaak van; Bovy, Arnaud G. - \ 2016
    Frontiers in Plant Science 7 (2016). - ISSN 1664-462X
    Alkaloids - Flavonoids - Introgression lines - QTL analysis - Tomato (Solanum lycopersicum)

    Semi-polar metabolites such as flavonoids, phenolic acids, and alkaloids are very important health-related compounds in tomato. As a first step to identify genes responsible for the synthesis of semi-polar metabolites, quantitative trait loci (QTLs) that influence the semi-polar metabolite content in red-ripe tomato fruit were identified, by characterizing fruits of a population of introgression lines (ILs) derived from a cross between the cultivated tomato Solanum lycopersicum and the wild species Solanum chmielewskii. By analyzing fruits of plants grown at two different locations, we were able to identify robust metabolite QTLs for changes in phenylpropanoid glycoconjugation on chromosome 9, for accumulation of flavonol glycosides on chromosome 5, and for alkaloids on chromosome 7. To further characterize the QTLs we used a combination of genome sequencing, transcriptomics and targeted metabolomics to identify candidate key genes underlying the observed metabolic variation.

    Tigridia arequipensis (Iridaceae: Tigridieae), a new species from South Peru
    Montesinos, D.B. ; Pauca, A. ; Revilla, I. - \ 2016
    Blumea 61 (2016)1. - ISSN 0006-5196 - p. 4 - 7.
    Tigridia arequipensis (Iridaceae: Tigridieae) is a new species found in the province of Arequipa (department of Arequipa), South Peru. It is unique by its white to pale white (or pale lilac) flowers, outer tepals with purplish maroon and dark yellow spots and stripes, and inner tepals with pale purplish and bluish spots and stripes. Tigridia arequipensis is morphologically similar to T. raimondii and T. philippiana, it differs by having longer basal leaves, narrower and larger bracts, and outer tepals ovate and longer fruits. Resumen Tigridia arequipensis (Iridaceae: Tigridieae) es una nueva especie encontrada en la provincia de Arequipa (departamento de Arequipa), Sur de Perú. Es única por sus flores blancas a blanco claras (o lila pálido), los tépalos externos con puntuaciones y líneas lila-marrones y los tépalos internos con puntuaciones y líneas lila pálido con puntuaciones y líneas azuladas. Tigridia arequipensis es morfológicamente similar a T. raimondii y T. philippiana, se difiere por tener hojas basales más alargadas, brácteas angostas y alargadas, los tépalos externos ovados y frutos más alargados.
    Iluminación LED en frutos de jitomate aumenta niveles de vitamina C
    Wageningen UR Glastuinbouw, ; Labrie, C.W. - \ 2013
    Flores y frutos de algunas cactáceas del norte de Moquegua
    Montesinos, D.B. - \ 2012
    Quepo 26 (2012). - ISSN 1022-5897 - p. 34 - 46.
    Small fruit market risks and oppertunities at European level
    Groot, Marianne - \ 2012
    International collaborative study on the occurrence of plasmid mediated quinolone resisitance in Salmonella enterica en Escherichia coli isolated from animals, humans, food and the environment in 13 European countries.
    Veldman, K.T. ; Cavaco, L.M. ; Mevius, D.J. ; Battisti, A. ; Botteldoorn, N. ; Bruneau, M. ; Cerny, T. ; Franco, A. ; Frutos Escobar, C. De; Guerra, B. ; Gutierrez, M. ; Hopkins, K. ; Myllyniemi, A.L. ; Perrin-Guyomard, A. ; Schroeter, A. ; Sunde, M. ; Wasyl, D. ; Aarestrup, F.M. - \ 2011
    Journal of Antimicrobial Chemotherapy 66 (2011)6. - ISSN 0305-7453 - p. 1278 - 1286.
    qnr genes - determinant qnrs1 - modifying enzyme - clinical isolate - united-kingdom - 1st report - enterobacteriaceae - prevalence - mechanisms - strains
    Objectives This study was initiated to collect retrospective information on the occurrence of plasmid-mediated quinolone resistance (PMQR) in Salmonella enterica and Escherichia coli isolates in Europe and to identify the responsible genes. Methods Databases of national reference laboratories containing MIC values for Salmonella and E. coli isolated between 1994 and 2009 in animals, humans, food and the environment from 13 European countries were screened for isolates exhibiting a defined quinolone resistance phenotype, i.e. reduced susceptibility to fluoroquinolones and nalidixic acid. PCR and sequence analysis were performed to identify the responsible PMQR genes. Results Screening of databases of 13 European countries resulted in a selection of 1215 Salmonella and 333 E. coli isolates. PMQR genes were identified in 59% of the Salmonella isolates and 15% of the E. coli isolates selected. In Salmonella, qnrS1 (n¿=¿125) and variants of qnrB (n¿=¿138) were frequently identified, whereas qnrA1 (n¿=¿3) and aac(6')-1b-cr (n¿=¿3) were rarely found. qnrD was detected in 22 Salmonella isolates obtained from humans and animals. In E. coli, qnrS1 was identified in 19 isolates and qnrB19 was found in one isolate. No qnrC or qepA genes were detected in either Salmonella or E. coli. Conclusions This study shows the occurrence and dissemination of PMQR genes in Salmonella and E. coli in Europe with a defined quinolone resistance phenotype. We also report the first detection of qnrD in Salmonella collected in Europe.
    Transcriptomic profiling of citrus fruit peel tissues reveals fundamental effects of phenylpropanoids and ethylene on induced resistance
    Ballester Frutos, A.R. ; Lafuente, M.T. ; Forment, J. ; Gadea, J. ; Vos, C.H.R. de; Bovy, A.G. ; Gonzalez-Candelas, L. - \ 2011
    Molecular Plant Pathology 12 (2011)9. - ISSN 1464-6722 - p. 879 - 897.
    penicillium-digitatum sacc - phenylalanine ammonia-lyase - plant defense responses - microarray data - acquired-resistance - decay resistance - gene-expression - uv-irradiation - infection - grapefruit
    Penicillium spp. are the major postharvest pathogens of citrus fruit in Mediterranean climatic regions. The induction of natural resistance constitutes one of the most promising alternatives to avoid the environmental contamination and health problems caused by chemical fungicides. To understand the bases of the induction of resistance in citrus fruit against Penicillium digitatum, we have used a 12k citrus cDNA microarray to study transcriptional changes in the outer and inner parts of the peel (flavedo and albedo, respectively) of elicited fruits. The elicitor treatment led to an over-representation of biological processes associated with secondary metabolism, mainly phenylpropanoids and cellular amino acid biosynthesis and methionine metabolism, and the down-regulation of genes related to biotic and abiotic stresses. Among phenylpropanoids, we detected the over-expression of a large subset of genes important for the synthesis of flavonoids, coumarins and lignin, especially in the internal tissue. Furthermore, these genes and those of ethylene biosynthesis showed the highest induction. The involvement of both phenylpropanoid and ethylene pathways was confirmed by examining changes in gene expression and ethylene production in elicited citrus fruit. Therefore, global results indicate that secondary metabolism, mainly phenylpropanoids, and ethylene play important roles in the induction of resistance in citrus fruit.
    Scanning electron microscopy of the interaction between Cryptococcus magnus and Colletotrichum gloeosporioides on papaya fruit = Microscopia eletrônica de varredura da interação entre Cryptococcus magnus e Colletotrichum gloeosporioides em frutos de mamão
    Capdeville, G. ; Souza, M.T. ; Santos, J.R.P. ; Miranda, S.P. ; Caetano, A.R. ; Falcao, R. ; Gomes, A.C.M.M. - \ 2007
    Pesquisa Agropecuaria Brasileira 42 (2007)11. - ISSN 0100-204X - p. 1537 - 1544.
    blue mold - postharvest biocontrol - botrytis-cinerea - apple fruit - yeasts - mode - pathogens - rot
    The objective of this work was to investigate possible modes of action of the yeast Cryptococcus magnus in controlling anthracnose (Colletotrichum gloeosporioides) on post harvested papaya fruits. Scanning electron microscopy was used to analyze the effect of the yeast on inoculations done after harvest. Results showed that C. magnus is able to colonize wound surfaces much faster than the pathogen, outcompeting the later for space and probably for nutrients. In addition, C. magnus produces a flocculent matrix, which affects hyphae integrity. The competition for space and the production of substances that affect hyphae integrity are among the most important modes of action of this yeast
    Interaction between functional domains of Bacillus thuringiensis insecticidal crystal proteins
    Rang, C. ; Vachon, V. ; Maagd, R.A. de; Villalon, M. ; Schwartz, J.L. ; Bosch, D. ; Frutos, R. ; Laprade, R. - \ 1999
    Applied and Environmental Microbiology 65 (1999)7. - ISSN 0099-2240 - p. 2918 - 2925.
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