Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Records 1 - 17 / 17

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    E-DATA: A Comprehensive Field Campaign to Investigate Evaporation Enhanced by Advection in the Hyper-Arid Altiplano
    Suárez, Francisco ; Lobos, Felipe ; La Fuente, Alberto De; Vilà-guerau De Arellano, Jordi ; Prieto, Ana ; Meruane, Carolina ; Hartogensis, Oscar - \ 2020
    Water 12 (2020)3. - ISSN 2073-4441
    In the endorheic basins of the Altiplano, water is crucial for sustaining unique ecological habitats. Here, the wetlands act as highly localized evaporative environments, and little is known about the processes that control evaporation. Understanding evaporation in the Altiplano is challenging because these environments are immersed in a complex topography surrounded by desert and are affected by atmospheric circulations at various spatial scales. Also, these environments may be subject to evaporation enhancement events as the result of dry air advection. To better characterize evaporation processes in the Altiplano, the novel Evaporation caused by Dry Air Transport over the Atacama Desert (E-DATA) field campaign was designed and tested at the Salar del Huasco, Chile. The E-DATA combines surface and airborne measurements to understand the evaporation dynamics over heterogeneous surfaces, with the main emphasis on the open water evaporation. The weather and research forecasting model was used for planning the instruments installation strategy to understand how large-scale air flow affects evaporation. Instrumentation deployed included: meteorological stations, eddy covariance systems, scintillometers, radiosondes and an unmanned aerial vehicle, and fiber-optic distributed temperature sensing. Additional water quality and CO2 fluxes measurements were carried out to identify the link between meteorological conditions and the biochemical dynamics of Salar del Huasco. Our first results show that, in the study site, evaporation is driven by processes occurring at multiple spatial and temporal scales and that, even in the case of available water and energy, evaporation is triggered by mechanical turbulence induced by wind. View Full-Text
    Identification de biomarqueurs de l'efficacité alimentaire chez le porc en croissance
    Gondret, Florence ; Skou Hedeman, Mette ; Messad, Farouk ; Verschuren, Lisanne ; Wang, Junjun ; Fuente, Gabriel de la; Gilbert, Hélène ; Jansman, A.J.M. - \ 2020
    In: 52èmes Journées de la Recherche Porcine. - IFIP, INRA - p. 20 - 24.
    Human to human transmission of arthropod-borne pathogens
    Martina, Byron E. ; Barzon, Luisa ; Pijlman, Gorben P. ; Fuente, José de la; Rizzoli, Annapaola ; Wammes, Linda J. ; Takken, Willem ; Rij, Ronald P. van; Papa, Anna - \ 2017
    Current Opinion in Virology 22 (2017). - ISSN 1879-6257 - p. 13 - 21.

    Human-to-human (H2H) transmitted arthropod-borne pathogens are a growing burden worldwide, with malaria and dengue being the most common mosquito-borne H2H transmitted diseases. The ability of vectors to get infected by humans during a blood meal to further propel an epidemic depends on complex interactions between pathogens, vectors and humans, in which human interventions and demographic and environmental conditions play a significant role. Herein, we discuss the distal and proximal drivers affecting H2H vector-borne pathogen transmission and identify knowledge gaps and future perspectives.

    The response regulator YycF inhibits expression of the fatty acid biosynthesis repressor FabT in Streptococcus pneumoniae
    Mohedano, Maria L. ; Amblar, Mónica ; La Fuente, Alicia De; Wells, Jerry M. ; López, Paloma - \ 2016
    Frontiers in Microbiology 7 (2016). - ISSN 1664-302X - 14 p.
    Essential response regulator - Fatty acid biosynthesis - Streptococcus pneumoniae - Transcriptional regulation - Two component systems

    The YycFG (also known as WalRK, VicRK, MicAB, or TCS02) two-component system (TCS) is highly conserved among Gram-positive bacteria with a low G+C content. In Streptococcus pneumoniae the YycF response regulator has been reported to be essential due to its control of pcsB gene expression. Previously we showed that overexpression of yycF in S. pneumoniae TIGR4 altered the transcription of genes involved in cell wall metabolism and fatty acid biosynthesis, giving rise to anomalous cell division and increased chain length of membrane fatty acids. Here, we have overexpressed the yycFG system in TIGR4 wild-type strain and yycF in a TIGR4 mutant depleted of YycG, and analyzed their effects on expression of proteins involved in fatty acid biosynthesis during activation of the TCS. We demonstrate that transcription of the fab genes and levels of their products were only altered in the YycF overexpressing strain, indicating that the unphosphorylated form of YycF is involved in the regulation of fatty acid biosynthesis. In addition, DNA-binding assays and in vitro transcription experiments with purified YycF and the promoter region of the FabTH-acp operon support a direct inhibition of transcription of the FabT repressor by YycF, thus confirming the role of the unphosphorylated form in transcriptional regulation.

    An O-methyltransferase modifies accumulation of methylated anthocyanins in seedlings of tomato
    Gomez Roldan, M.V. ; Outchkourov, N.S. ; Houwelingen, A.M.M.L. van; Lammers, M. ; Romero Fuente, I. ; Ziklo, N. ; Aharoni, A. ; Hall, R.D. ; Beekwilder, M.J. - \ 2014
    The Plant Journal 80 (2014)4. - ISSN 0960-7412 - p. 695 - 708.
    plant transformation - transcription factor - petunia-hybrida - fruit - biosynthesis - expression - protein - system - metabolome - infection
    Anthocyanins contribute to the appearance of fruit by conferring to them a red, blue or purple colour. In a food context, they have also been suggested to promote consumer health. In purple tomato tissues, such as hypocotyls, stems and purple fruits, various anthocyanins accumulate. These molecules have characteristic patterns of modification, including hydroxylations, methylations, glycosylations and acylations. The genetic basis for many of these modifications has not been fully elucidated, and nor has their role in the functioning of anthocyanins. In this paper, AnthOMT, an O-methyltransferase (OMT) mediating the methylation of anthocyanins, has been identified and functionally characterized using a combined metabolomics and transcriptomics approach. Gene candidates were selected from the draft tomato genome, and their expression was subsequently monitored in a tomato seedling system comprising three tissues and involving several time points. In addition, we also followed gene expression in wild-type red and purple transgenic tomato fruits expressing Rosea1 and Delila transcription factors. Of the 57 candidates identified, only a single OMT gene showed patterns strongly correlating with both accumulation of anthocyanins and expression of anthocyanin biosynthesis genes. This candidate (AnthOMT) was compared to a closely related caffeoyl CoA OMT by recombinant expression in Escherichia coli, and then tested for substrate specificity. AnthOMT showed a strong affinity for glycosylated anthocyanins, while other flavonoid glycosides and aglycones were much less preferred. Gene silencing experiments with AnthOMT resulted in reduced levels of the predominant methylated anthocyanins. This confirms the role of this enzyme in the diversification of tomato anthocyanins.
    Identification and characterization of MYB-bHLH-WD40 regulatory complexes controlling proanthocyanidin biosynthesis in strawberry (Fragaria 9 ananassa) fruits
    Schaart, J.G. ; Dubois, C. ; Romero de la Fuente, I. ; Houwelingen, A.M.M.L. van; Vos, R.C.H. de; Jonker, H.H. ; Xu, W. ; Routaboul, J.M. ; Lepinice, L. ; Bovy, A.G. - \ 2013
    New Phytologist 197 (2013)2. - ISSN 0028-646X - p. 454 - 467.
    r2r3-myb transcription factor - arabidopsis-thaliana - anthocyanin biosynthesis - gene encodes - flavonoid biosynthesis - negative regulator - domain protein - factor family - myb domain - expression
    •Strawberry (Fragaria × ananassa) fruits contain high concentrations of flavonoids. In unripe strawberries, the flavonoids are mainly represented by proanthocyanidins (PAs), while in ripe fruits the red-coloured anthocyanins also accumulate. Most of the structural genes leading to PA biosynthesis in strawberry have been characterized, but no information is available on their transcriptional regulation. In Arabidopsis thaliana the expression of the PA biosynthetic genes is specifically induced by a ternary protein complex, composed of AtTT2 (AtMYB123), AtTT8 (AtbHLH042) and AtTTG1 (WD40-repeat protein).
    •A strategy combining yeast-two-hybrid screening and agglomerative hierarchical clustering of transcriptomic and metabolomic data was undertaken to identify strawberry PA regulators.
    •Among the candidate genes isolated, four were similar to AtTT2, AtTT8 and AtTTG1 (FaMYB9/FaMYB11, FabHLH3 and FaTTG1, respectively) and two encode putative negative regulators (FaMYB5 and FabHLH3∆). Interestingly, FaMYB9/FaMYB11, FabHLH3 and FaTTG1 were found to complement the tt2-1, tt8-3 and ttg1-1 transparent testa mutants, respectively. In addition, they interacted in yeast and activated the Arabidopsis BANYULS (anthocyanidin reductase) gene promoter when coexpressed in Physcomitrella patens protoplasts.
    •Taken together, these results demonstrated that FaMYB9/FaMYB11, FabHLH3 and FaTTG1 are the respective functional homologues of AtTT2, AtTT8 and AtTTG1, providing new tools for modifying PA content and strawberry fruit quality.
    Voorkomen van uitval bij Impatiens walleriana onder veldcondities
    Hofland-Zijlstra, J.D. ; Vries, R.S.M. de; Wensveen, W. van; Fuente van Bentem, S. de la - \ 2012
    Bleiswijk : Wageningen UR Glastuinbouw (Rapporten GTB 1160) - 32
    potplanten - sierplanten - impatiens walleriana - ornamentele waarde - bodempathogenen - ziektebestrijdende teeltmaatregelen - peronospora farinosa - vollegrondsteelt - pot plants - ornamental plants - impatiens walleriana - ornamental value - soilborne pathogens - cultural control - peronospora farinosa - outdoor cropping
    Abstract Wageningen UR Greenhouse Horticulture, together with Syngenta Seeds performed field trials with fungicides and fertilizing products to prolong the consumer value of Impatiens walleriana (busy lily). This project was funded by the Dutch Horticultural Board. The plants were situated on two field locations, both in the field and in pots to follow the natural disease development with and without interaction with soil pathogens (e.g. downy mildew). The results of this study show that Ridomil Gold is effective against early infestation from soil propagules of downy mildew, but there is no effectivity against leaf infections. Products based on phoshite (potassium phosphite, product X of Everris) slow down the infection process with a few weeks. Especially, later in the season when the conidia are most dominantly present. It is recommended to screen multiple products which are effective against resting spores of downy mildew. Sensitive Impatiens species should preferably be planted early in the season and not directly in soil with high risk of surviving propagules.
    Voorkomen van uitval bij Impatiens walleriana onder veldcondities (groeiseizoen 2011)
    Vries, R.S.M. de; Hofland-Zijlstra, J.D. ; Fuente van Bentem, S. de la - \ 2012
    impatiens walleriana - meeldauw - fungiciden - plantenziektebestrijding - sierteelt - tuinplanten - glastuinbouw - impatiens walleriana - mildews - fungicides - plant disease control - ornamental horticulture - bedding plants - greenhouse horticulture
    Poster met resultaten van onderzoek naar gewasbeschermingmiddelen tegen valse meeldauw in Impatiens walleriana.
    Luchas y defensas escondidas. Pluralismo legal y cultural como una práctica de resistencia creativa en la gestión local del agua en los Andes
    Boelens, R.A. - \ 2011
    Anuario de Estudios Americanos 68 (2011)2. - ISSN 0210-5810 - p. 673 - 703.
    En los Andes, los derechos de agua se materializan en procesos de lucha social. El artículo examina cómo la lucha por el agua de los colectivos locales no puede comprenderse sin su enraizamiento en subcorrientes dinámicas: los cimientos multicapas, a menudo ocultos, de los derechos de agua. Aquí se entrelazan estrategias comunitarias y fuentes socio-legales plurales. Las subcorrientes alimentan los socio-territorios y las culturas hidráulicas, estableciendo las bases para la defensa de los derechos locales hacia redes político-legales multi-escala. En la práctica, los arreglos y derechos propios a menudo están disfrazados por medio de escudos y estrategias de mimetismo (o imitación), que también permiten hacer uso de los medios de poder dominantes. La creación y la proliferación subsuperficiales de los repertorios sociolegales locales constituyen una importante fuente de defensa contra la usurpación de los derechos de agua y las políticas disciplinarias.
    Nieuwe middelen tegen valse meeldauw in Impatiens walleriana
    Hofland-Zijlstra, J.D. ; Noort, F.R. van; Bohne, S. ; Hamelink, R. ; Wensveen, W. van; Fuente van Bentem, S. de la; Sanders, M. - \ 2011
    Bleiswijk : Wageningen UR Glastuinbouw (Rapporten GTB 1081) - 26
    meeldauw - oömyceten - plasmopara obducens - impatiens walleriana - plantenziekteverwekkende schimmels - bestrijdingsmethoden - veldproeven - kasproeven - nederlands - mildews - oomycetes - plasmopara obducens - impatiens walleriana - plant pathogenic fungi - control methods - field tests - greenhouse experiments - dutch
    Abstract Wageningen UR Greenhouse Horticulture, together with Syngenta Seeds tested various combinations of agents for their effects to increase plant defense against infection of downy mildew in the consumer phase. All means were dosed 2-3 times during the growers phase. There are three new products, which were found to strengthen Impatiens plants against infection by downy mildew. It is still unclear how these products can be combined with other agents that also control pathogenic soil fungi so that failure occurs less rapidly under field conditions at high disease pressure. In 2011, this research will be continued at two field locations.
    Aanbevelingen voor beheersing van valse meeldauw in Impatiens walleriana
    Hofland-Zijlstra, J.D. ; Noort, F.R. van; Fuente, S. de la; Sanders, M. - \ 2010
    Gewasnieuws Zomerbloemen 13 (2010)2. - p. 3 - 3.
    Beheersing van valse meeldauw in Impatiens
    Hofland-Zijlstra, J.D. ; Noort, F.R. van; Fuente van Bentem, S. de la; Stolte, A. ; Sanders, M. ; Weijtmans, K. - \ 2010
    meeldauw - impatiens - impatiens walleriana - schimmels - beschermingsmiddelen - mildews - impatiens - impatiens walleriana - fungi - protectants
    Poster over de beheersing van valse meeldauw in Impatiens. De veelvuldig als perkgoed verkochte zomerbloeier Impatiens walleriana viel in de zomer van 2007 en 2008 veelvuldig uit door valse meeldauw. In 2009 is hier met behulp van moleculaire technieken onderzocht welke schimmel(s) verantwoordelijk zijn voor uitval, of deze ook vanuit de grond infectie kan veroorzaken en met welke middelen deze te bestrijden is. Deze poster geeft een overzicht van de resultaten.
    Beheersing van valse meeldauw in Impatiens
    Hofland-Zijlstra, J.D. ; Noort, F.R. van; Böhne, S. ; Fuente van Bentem, S. de la; Weijtmans, K. ; Sanders, M. - \ 2010
    Bleiswijk : Wageningen UR Glastuinbouw / Syngenta Seeds B.V. (Rapport / Wageningen UR Glastuinbouw 330) - 17
    bloementeelt - impatiens walleriana - peronospora farinosa - preventie - gewasbescherming - zomerbloemen - floriculture - impatiens walleriana - peronospora farinosa - prevention - plant protection - summer flowers
    De éénjarige zomerbloeier Impatiens walleriana vormt een belangrijk onderdeel in het assortiment van perkgoed. Ondermeer in 2007 waren er door de natte weersomstandigheden grote problemen met uitval door valse meeldauw in de consumentfase. Hierdoor kwam de vraag of er tijdens de opkweek niet meer preventieve maatregelen genomen kunnen worden om infectie door valse meeldauw onder vochtige omstandigheden te verminderen of te voorkomen. Daarnaast was het onbekend of de besmetting met valse meeldauw op Nederlandse locaties veroorzaakt wordt door één soort of meerdere soorten. In een literatuurstudie is alle beschikbare informatie over valse meeldauw en mogelijkheden voor beheersen/ bestrijding samengevat
    Aanbevelingen voor beheersing van valse meeldauw in Impatiens walleriana
    Hofland-Zijlstra, J.D. ; Noort, F.R. van; Fuente, S. de la; Sanders, M. - \ 2010
    Gewasnieuws Eenjarige zomerbloeiers 13 (2010)1. - p. 2 - 2.
    Los patrones de movilidad de las personas: Una fuente de conocimiento geoespacial
    Wachowicz, M. ; Vazquez, A.H. ; Ballari, D.E. ; Orellana, D.A. ; Rodriguez, A. - \ 2009
    Novática: Revista de la Asociación de Técnicos de Informática 2009 (2009)198. - ISSN 0211-2124 - p. 18 - 23.
    Impatiens weerbaar tegen valse meeldauw zonder verlies van plantkwaliteit
    Hofland-Zijlstra, J.D. ; Noort, F.R. van; Fuente van Bentem, S. de la; Sanders, M. - \ 2009
    Gewasnieuws Eenjarige zomerbloeiers 12 (2009)4. - p. 2 - 2.
    Protein phosphatase 5 and Heat Shock Protein 90 are components of the tomato I-2 disease resistance protein complex
    Vries, K.J. de; Fuente van Bentem, S. de la; Vossen, J.H. ; Tameling, W.I.L. ; Dekker, H.L. ; Koster, C.G. de; Haring, M.A. ; Cornelissen, B.J.C. ; Takken, F.L.W. - \ 2005
    In: Book of Abstracts XII International Congress on Molecular Plant-Microbe Interactions, Mérida, México, 14-19 December 2005 - p. 205 - 205.
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