Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Reprint of : Microbial food safety in the 21st century: Emerging challenges and foodborne pathogenic bacteria
    Franz, Charles M.A.P. ; Besten, Heidy M.W. den; Böhnlein, Christina ; Gareis, Manfred ; Zwietering, Marcel H. ; Fusco, Vincenzina - \ 2019
    Trends in Food Science and Technology 84 (2019). - ISSN 0924-2244 - p. 34 - 37.
    Food safety - Microbial risk assessment - Pathogens
    Microbial food safety in the 21st century : Emerging challenges and foodborne pathogenic bacteria
    Franz, Charles M.A.P. ; Besten, Heidy M.W. den; Böhnlein, Christina ; Gareis, Manfred ; Zwietering, Marcel H. ; Fusco, Vincenzina - \ 2018
    Trends in Food Science and Technology 81 (2018). - ISSN 0924-2244 - p. 155 - 158.
    Food safety - Microbial risk assessment - Pathogens
    Food safety aspects on ethnic foods : Toxicological and microbial risks
    Fusco, Vincenzina ; Besten, H.M.W. den; Logrieco, Antonio F. ; Rodriguez, Fernando Perez ; Skandamis, Panagiotis N. ; Stessl, Beatrix ; Teixeira, Paula - \ 2015
    Current Opinion in Food Science 6 (2015). - ISSN 2214-7993 - p. 24 - 32.

    Ethnic foods are becoming popular worldwide. Nevertheless, foodborne outbreaks and food recalls due to the contamination of these foods with pathogenic agents, toxins, undeclared allergens and hazardous chemical compounds are increasing in recent years together with their growing popularity. In this context, ad hoc prevention and control strategies are needed, to assess and face the emerging microbiological and toxicological risks associated with the consumption of ethnic foods.

    A Versatile Toolkit to Produce Sensitive FRET Biosensors to Visualize Signaling in Time and Space
    Fritz, R.D. ; Letzelter, M. ; Reimann, A. ; Martin, K. ; Fusco, L. ; Ritsma, L. ; Ponsioen, B. ; Fluri, E. ; Schulte-Merker, S. ; Rheenen, J. ; Pertz, O. - \ 2013
    Science Signaling 6 (2013)285. - ISSN 1945-0877
    cyan fluorescent protein - rho-family gtpases - dynamic-range - living cells - in-vivo - activation - kinase - cdc42 - indicators - reporters
    Genetically encoded, ratiometric biosensors based on fluorescence resonance energy transfer (FRET) are powerful tools to study the spatiotemporal dynamics of cell signaling. However, many biosensors lack sensitivity. We present a biosensor library that contains circularly permutated mutants for both the donor and acceptor fluorophores, which alter the orientation of the dipoles and thus better accommodate structural constraints imposed by different signaling molecules while maintaining FRET efficiency. Our strategy improved the brightness and dynamic range of preexisting RhoA and extracellular signal–regulated protein kinase (ERK) biosensors. Using the improved RhoA biosensor, we found micrometer-sized zones of RhoA activity at the tip of F-actin bundles in growth cone filopodia during neurite extension, whereas RhoA was globally activated throughout collapsing growth cones. RhoA was also activated in filopodia and protruding membranes at the leading edge of motile fibroblasts. Using the improved ERK biosensor, we simultaneously measured ERK activation dynamics in multiple cells using low-magnification microscopy and performed in vivo FRET imaging in zebrafish. Thus, we provide a construction toolkit consisting of a vector set, which enables facile generation of sensitive biosensors.
    Properties of the recombinant a-glucosidase from Sulfolobus solfataricus in relation to starch processing
    Martino, A. ; Schiraldi, C. ; Fusco, S. ; Lernia, I. Di; Costabile, T. ; Pellicano, T. ; Marotta, M. ; Generoso, M. ; Oost, J. van der; Sensen, C.W. - \ 2001
    Journal of Molecular Catalysis. B, Enzymatic 11 (2001). - ISSN 1381-1177 - p. 787 - 794.
    An -glucosidase activity (EC isolated from Sulfolobus solfataricus strain MT-4 was characterised and found of interest at industrial level in the saccharification step of hydrolysis process of starch. The gene encoding for the enzyme was expressed in Escherichia coli BL21 (DE3) with a yield of 87.5 U/g of wet biomass. The recombinant cytosolic enzyme was purified to homogeneity with a rapid purification procedure employing only steps of selective and progressive thermal precipitations with a final yield of 75.4nd a purification of 14.5-fold. The properties of this thermophilic -glucosidase were compared with those of the -glucosidase of a commercial preparation from Aspergillus niger used in the starch processing.
    Identification and molecular characterization of the first a-xylosidase from an Archaeon
    Moracci, M. ; Cobucci-Ponzano, B. ; Trincone, A. ; Fusco, S. ; Rosa, M. de; Oost, J. van der; Sensen, C.W. ; Charlebois, R.L. ; Rossi, M. - \ 2000
    Journal of Biological Chemistry 275 (2000). - ISSN 0021-9258 - p. 22082 - 22089.
    We here report the first molecular characterization of an -xylosidase (XylS) from an Archaeon. Sulfolobus solfataricus is able to grow at temperatures higher than 80 °C on several carbohydrates at acidic pH. The isolated xylS gene encodes a monomeric enzyme homologous to -glucosidases, -xylosidases, glucoamylases and sucrase-isomaltases of the glycosyl hydrolase family 31. xylS belongs to a cluster of four genes in the S. solfataricus genome, including a -glycosidase, an hypothetical membrane protein homologous to the major facilitator superfamily of transporters, and an open reading frame of unknown function. The -xylosidase was overexpressed in Escherichia coli showing optimal activity at 90 °C and a half-life at this temperature of 38 h. The purified enzyme follows a retaining mechanism of substrate hydrolysis, showing high hydrolytic activity on the disaccharide isoprimeverose and catalyzing the release of xylose from xyloglucan oligosaccharides. Synergy is observed in the concerted in vitro hydrolysis of xyloglucan oligosaccharides by the -xylosidase and the -glycosidase from S. solfataricus. The analysis of the total S. solfataricus RNA revealed that all the genes of the cluster are actively transcribed and that xylS and orf3 genes are cotranscribed.
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