Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Actors in a Masala Movie: Fieldnotes on the NGO Tsunami response in eastern Sri Lanka
    Gaasbeek, T.J. - \ 2010
    In: Tsunami Recovery in Sri Lanka. Ethnic and regional dimensions / McGilvray, D.B., Gamburd, M.R., London, New York : Routledge (Routledge contemporary South Asia series ) - ISBN 9780415778770 - p. 125 - 142.
    Bridging troubled waters? : everyday inter-ethnic interaction in a content of violent conflict in Kottiyar Patty, Trincomalee, Sri Lanka
    Gaasbeek, T. - \ 2010
    Wageningen University. Promotor(en): Georg Frerks; Linden Vincent. - S.l. : s.n. - ISBN 9789085856467 - 370
    rampen - conflict - oorlog - etniciteit - etnische groepen - agressief gedrag - voorlichting - irrigatie - waterbeheer - huwelijk - sociologie - sociale differentiatie - sri lanka - zuid-azië - disasters - conflict - war - ethnicity - ethnic groups - aggressive behaviour - extension - irrigation - water management - marriage - sociology - social differentiation - sri lanka - south asia - cum laude
    cum laude graduation (with distinction)
    Nucleases Encoded by Integraded Elements CJIE2 and CJIE4 Inhibit Natural Transformation of Campylobacter Jejuni
    Gaasbeek, E.J. ; Wagenaar, J.A. ; Guilhabert, M.R. ; Putten, J.P. van; Parker, C.T. ; Wal, F.J. van der - \ 2010
    Journal of Bacteriology 192 (2010)4. - ISSN 0021-9193 - p. 936 - 941.
    serratia-marcescens endonuclease - horizontal gene-transfer - diversity - bacteria - sequence - dnase - identification - mutagenesis - mechanism - strains
    The species Campylobacter jejuni is naturally competent for DNA uptake; nevertheless, nonnaturally transformable strains do exist. For a subset of strains we previously showed that a periplasmic DNase, encoded by dns, inhibits natural transformation in C. jejuni. In the present study, genetic factors coding for DNase activity in absence of dns were identified. DNA arrays indicated that nonnaturally transformable dns-negative strains contain putative DNA/RNA non-specific endonucleases encoded by CJE0566 and CJE1441 of strain RM1221. These genes are located on C. jejuni integrated element 2 and 4. Expression of CJE0566 and CJE1441 from strain RM1221 and a homologous gene from strain 07479 in DNase-negative Escherichia coli and C. jejuni strains indicated that these genes code for DNases. Genetic transfer of the genes to a naturally transformable C. jejuni strain resulted in a decreased efficiency of natural transformation. Modelling suggests that the C. jejuni DNases belong to the Serratia nuclease family. Overall, the data indicate that the acquisition of prophage encoded DNA/RNA non-specific endonucleases inhibits the natural transformability of C. jejuni through hydrolysis of DNA
    Functional Characterization of Excision Repair and RecA-Dependent Recombinational DNA Repair in Campylobacter jejuni
    Gaasbeek, E.J. ; Wal, F.J. van der; Putten, J.P.M. van; Boer, P. ; Graaf-van Bloois, L. van der; Boer, A.G. de; Vermaning, B.J. ; Wagenaar, J.A. - \ 2009
    Journal of Bacteriology 191 (2009)12. - ISSN 0021-9193 - p. 3785 - 3793.
    escherichia-coli - mismatch-repair - natural transformation - helicobacter-pylori - genome sequence - haemophilus-influenzae - replication forks - ultraviolet-light - genetic diversity - clinical isolate
    The presence and functionality of DNA repair mechanisms in Campylobacter jejuni are largely unknown. In silico analysis of the complete translated genome of C. jejuni NCTC 11168 suggests the presence of genes involved in methyl-directed mismatch repair (MMR), nucleotide excision repair, base excision repair (BER), and recombinational repair. To assess the functionality of these putative repair mechanisms in C. jejuni, mutS, uvrB, ung, and recA knockout mutants were constructed and analyzed for their ability to repair spontaneous point mutations, UV irradiation-induced DNA damage, and nicked DNA. Inactivation of the different putative DNA repair genes did not alter the spontaneous mutation frequency. Disruption of the UvrB and RecA orthologues, but not the putative MutS or Ung proteins, resulted in a significant reduction in viability after exposure to UV irradiation. Assays performed with uracil-containing plasmid DNA showed that the putative uracil-DNA glycosylase (Ung) protein, important for initiation of the BER pathway, is also functional in C. jejuni. Inactivation of recA also resulted in a loss of natural transformation. Overall, the data indicate that C. jejuni has multiple functional DNA repair systems that may protect against DNA damage and limit the generation of genetic diversity. On the other hand, the apparent absence of a functional MMR pathway may enhance the frequency of on-and-off switching of phase variable genes typical for C. jejuni and may contribute to the genetic heterogeneity of the C. jejuni population.
    A DNase encoded by integrated element CJIE1 inhibits natural transformation of Campylobacter jejuni
    Gaasbeek, E.J. ; Wagenaar, J.A. ; Guilhabert, M.R. ; Wösten, M.M.S.M. ; Putten, J.P.M. van; Graaf-van Bloois, L. van der; Parker, C.T. ; Wal, F.J. van der - \ 2009
    Journal of Bacteriology 191 (2009)7. - ISSN 0021-9193 - p. 2296 - 2306.
    horizontal gene-transfer - guillain-barre-syndrome - escherichia-coli - aeromonas-hydrophila - extracellular dnases - vibrio-cholerae - diversity - bacteria - reveals - virulence
    The species Campylobacter jejuni (C. jejuni) is considered naturally competent for DNA uptake and displays strong genetic diversity. Nevertheless, non-naturally transformable strains and several relatively stable clonal lineages exist. In the present study, the molecular mechanism responsible for the non-natural transformability of a subset of C. jejuni strains was investigated. Comparative genome hybridization indicated that the C. jejuni Mu-like prophage integrated element 1 (CJIE1) was more abundant in non-naturally transformable C. jejuni strains than in naturally transformable strains. Analysis of CJIE1 indicated the presence of dns (CJE0256), which is annotated as an extracellular deoxyribonuclease. DNase assays using a defined dns mutant and a dns- strain expressing Dns from a plasmid indicated Dns as an endogenous DNase. The DNA hydrolyzing activity directly correlated with the natural transformability of the knockout mutant and the dns- strain expressing Dns from a plasmid. Analysis of a broader set of strains indicated that the majority of non-naturally transformable strains expressed DNase activity, while all naturally competent strains lacked this activity. The inhibition of natural transformation in C. jejuni via endogenous DNase activity possibly contributes to the formation of stable lineages in the C. jejuni population
    Molecular mechanisms involved in diversity and prservation of clonality in Champylobacter jejuni
    Gaasbeek, E.J. ; Wal, F.J. van der; Putten, J.P.M. ; Wagenaar, J.A. - \ 2008
    Inhibition of natural transformation through an endogenous DNase in Campylobacter jejuni
    Gaasbeek, E.J. ; Wagenaar, J.A. ; Guilhabert, M.R. ; Wosten, M.M. ; Putten, J.P.M. ; Graaf-van Bloois, L. van der - \ 2007
    A defect in natural transformation contributes to clonality in Campylobacter jejuni
    Gaasbeek, E.J. ; Wal, F.J. van der; Putten, J.P.M. ; Wagenaar, J.A. - \ 2007
    A defect in natural transformation contributes to clonality in Campylobacter jejuni
    Gaasbeek, E.J. ; Wal, F.J. van der; Putten, J.P. van; Wagenaar, J.A. - \ 2007
    A Defect in Natural Transformation Contributes to Clonality in Campylobacter jejuni
    Gaasbeek, E.J. ; Wal, F.J. van der; Putten, J.P.M. ; Bleumink-Pluym, N.M. ; Wagenaar, J.A. - \ 2007
    Exploration of projective techniques to unravel health perception
    Sijtsema, S.J. ; Linnemann, A.R. ; Backus, G.B.C. ; Jongen, W.M.F. ; Gaasbeek, A.F. van; Dagevos, H. - \ 2007
    British Food Journal 109 (2007)6. - ISSN 0007-070X - p. 443 - 456.
    food - attitudes - choice
    Purpose - This paper seeks to explore the design, organisation and application of group discussions in which projective techniques (expressive and associative) are used to unravel health perception of consumers in cognitive and affective terms. Design/methodology/approach - A trained moderator led four group discussions in which 24 Dutch women, divided into two groups of six women aged between 50 and 65, and two groups of women with young children (0-7 years old) participated. By means of expressive and associative techniques participants discuss health and food based on non-verbal expressions, namely, drawings and abstract paintings made by themselves. The participants selected and discussed relevant terms related to food and health based on their interpretations and associations of images. Findings - Participants related healthy to feeling free and happy (affective) and implied a balance between being active and passive. "Health" and "food" are associated with terms of nature (e.g. season, water and sun), specific products (vegetables and fruits), ingredients (vitamins, fibres, minerals) and no additives (cognitive). Originality/value - The expressive and associative group discussions proved to be a promising, fascinating and participant-friendly approach to gain an insight into the affective and the cognitive aspects that consumers relate to health-promoting product characteristics.
    Irrigation in conflict : Cross-boundary water management in a context of violent conflict in East Sri Lanka
    Gaasbeek, T.J. - \ 2005
    Mededingingsrecht in Nederland
    Gaasbeek, P.B. ; Meulen, B.M.J. van der - \ 2003
    In: Integraal Mededingingsrecht / Gaasbeek, P.B., van der Meulen, B.M.J., 's-Gravenhage : Elsevier - ISBN 9789059018426 - p. 11 - 16.
    Integraal Mededingingsrecht
    Gaasbeek, P.B. ; Meulen, B.M.J. van der - \ 2003
    's-Gravenhage : Elsevier (Elsevier Juridisch ) - ISBN 9789059018426 - 1071 p.
    Variables Influencing Food Perception Reviewed for Consumer-Oriented Product Development
    Sijtsema, S.J. ; Linnemann, A.R. ; Gaasbeek, T. ; Dagevos, H. ; Jongen, W.M.F. - \ 2002
    Critical Reviews in Food Science and Nutrition 42 (2002)6. - ISSN 1040-8398 - p. 565 - 581.
    health - consumption - attitudes - choice
    Consumer wishes have to be translated into product characteristics to implement consumer-oriented product development. Before this step can be made, insight in food-related behavior and perception of consumers is necessary to make the right, useful, and successful translation. Food choice behavior and consumers' perception are studied in many disciplines. Models of food behavior and preferences therefore were studied from a multidisciplinary perspective. Nearly all models structure the determinants related to the person, the food, and the environment. Consequently, the overview of models was used as a basis to structure the variables influencing food perception into a model for consumer-oriented product development. To this new model, referred to as food perception model, other variables like time and place as part of consumption moment were added. These are important variables influencing consumers' perception, and therefore of increasing importance to consumer-oriented product development nowadays. In further research, the presented food perception model is used as a tool to implement successful consumer-oriented product development.
    Food Consumption, Food Safety and Risk Perception
    Ophem, J. van; Dagevos, H. ; Gaasbeek, A.F. van - \ 2002
    In: Changes at the Other End of the Chain: everyday consumption in a multidisciplinary perspective / Butijn, C.A.A., Groot-Marcus, J.P., van der Linden, M., Steenbekkers, L.P.A., Terpstra, P.M.J., Aachen : Shaker Verlag - ISBN 9789042301825 - p. 107 - 121.
    Belevingsconsumenten: variatie in zorgen om voedselveiligheid
    Dagevos, H. ; Gaasbeek, A.F. van; Verhoeven, M. - \ 2001
    INDEX, feiten en cijfers over onze samenleving 8 (2001)8. - ISSN 1380-7447 - p. 2 - 3.
    consumenten - voedingsmiddelen - voedselveiligheid - houding van consumenten - statistiek - consumers - foods - food safety - consumer attitudes - statistics
    Hoe veilig vinden consumenten hun voeding werkelijk?
    Performance benchmarking van ketennetwerken
    Vlieger, J.J. de; Gaasbeek, A.F. van; Hack, M.D. ; Wolters, A.M. ; Rie, J.P.P.F. van; Wolbrink, M.A.B. ; Heijden, C.H.T.M. van der; Roes, C.B. - \ 2001
    Den Haag : LEI - ISBN 9789052426907 - 42
    kwaliteitscontroles - werkorganisatie - organisaties - ketenmanagement - quality controls - organization of work - organizations - supply chain management
    Voedselveiligheid: communiceren met beleid
    Folbert, J.P. ; Gaasbeek, A.F. van; Kroon, S.M.A. van der - \ 2001
    Den Haag : LEI - ISBN 9789052426754 - 73
    voedselveiligheid - communicatie - voorlichting - risicoschatting - risicogedrag - risicovermindering - regering - consumenten - food safety - communication - extension - risk assessment - risk behaviour - risk reduction - government - consumers
    Waar in het bronsgroen eikenhout
    Ypma, M.E. ; Gaasbeek, A.F. van - \ 2001
    Den Haag : LEI - ISBN 9789052426839 - 91
    landschap - kwaliteit - landbouw - tuinbouw - certificering - milieuwetgeving - agrarische bedrijfsvoering - landbouwgrond - natuurbescherming - nederland - limburg - landscape - quality - agriculture - horticulture - certification - environmental legislation - farm management - agricultural land - nature conservation - netherlands - limburg
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