Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Glass-formingproperty of hydroxyectoine is the cause of its superior function as a desiccation protectant
Tanne, C. ; Golovina, E.A. ; Hoekstra, F.A. ; Meffert, A. ; Galinski, E.A. - \ 2014
Frontiers in Microbiology 5 (2014). - ISSN 1664-302X - 13 p.
compatible solute hydroxyectoine - escherichia-coli - chromohalobacter-salexigens - transition temperatures - alternating copolymers - halophilic eubacteria - trehalose dihydrate - ectoine hydroxylase - pseudomonas-putida - sugar glasses
We were able to demonstrate that hydroxyectoine, in contrast to ectoine, is a good glass-forming compound. Fourier transform infrared and spin label electron spin resonance studies of dry ectoine and hydroxyectoine have shown that the superior glass-forming properties of hydroxyectoine result from stronger intermolecular H-bonds with the OH group of hydroxyectoine. Spin probe experiments have also shown that better molecular immobilization in dry hydroxyectoine provides better redox stability of the molecules embedded in this dry matrix. With a glass transition temperature of 87°C (vs. 47°C for ectoine) hydroxyectoine displays remarkable desiccation protection properties, on a par with sucrose and trehalose. This explains its accumulation in response to increased salinity and elevated temperature by halophiles such as Halomonas elongata and its successful application in “anhydrobiotic engineering” of both enzymes and whole cells.
Sulfidogenesis under extremely haloalkaline conditions by Desulfonatronospira thiodismutans gen. nov., sp. nov., and Desulfonatronospira delicata sp. nov. - a novel lineage of Deltaproteobacteria from hypersaline soda lakes
Sorokin, D.Y. ; Tourova, T.P. ; Henstra, A.M. ; Stams, A.J.M. ; Galinski, E.A. ; Muyzer, G. - \ 2008
Microbiology 154 (2008)Pt 5. - ISSN 1350-0872 - p. 1444 - 1453.
sulfate-reducing bacterium - inorganic sulfur-compounds - deoxyribonucleic acid - disproportionation - california - reduction - diversity - sediments - pathways - growth
High rates of sulfidogenesis were observed in sediments from hypersaline soda lakes. Anaerobic enrichment cultures at 2 M Na(+) and pH 10 inoculated with sediment samples from these lakes produced sulfide most actively with sulfite and thiosulfate as electron acceptors, and resulted in the isolation of three pure cultures of extremely natronophilic sulfidogenic bacteria. Strain ASO3-1 was isolated using sulfite as a sole substrate, strain AHT 8 with thiosulfate and formate, and strain AHT 6 with thiosulfate and acetate. All strains grew in a mineral soda-based medium by dismutation of either sulfite or thiosulfate, as well as with sulfite, thiosulfate and sulfate as acceptors, and H(2) and simple organic compounds as electron donors. The acetyl-CoA pathway was identified as the pathway for inorganic carbon assimilation by strain ASO3-1. All strains were obligately alkaliphilic, with an optimum at pH 9.5-10, and grew in soda brines containing 1-4 M total Na(+) (optimum at 1.0-2.0 M). The cells accumulated high amounts of the organic osmolyte glycine betaine. They formed a new lineage within the family Desulfohalobiaceae (Deltaproteobacteria), for which the name Desulfonatronospira gen. nov. is proposed. Strains ASO3-1(T) and AHT 8 from Kulunda Steppe formed Desulfonatronospira thiodismutans sp. nov., and strain AHT 6(T) from Wadi al Natrun is suggested as Desulfonatronospira delicata sp. nov
Scenarios on forest management in the Czech Republic, Hungary, Poland and Ukraine
Schelhaas, M.J. ; Cerny, M. ; Buksha, I. ; Cienciala, E. ; Csoka, P. ; Galinski, W. ; Karjalainen, T. ; Kolozs, L. ; Nabuurs, G.J. ; Pasternak, V. ; Pussinen, A. ; Sódor, M. ; Wawrzoniak, J. - \ 2004
Leiden / Boston : Brill (European Forest Institute research report 17) - ISBN 9004137270 - 107
bosbedrijfsvoering - duurzaamheid (sustainability) - klimaatverandering - hout - centraal-europa - forest management - sustainability - climatic change - wood - central europe
This research report gives insight into possible future development directions of the forests of CEC and NIS countries in order to contribute to current discussions about sustainability, the future biological potential, management options and impacts of environmental changes. With a large scale scenario model (EFISCEN), a quantitative assessment of future wood production in Czech Republic, Poland, Hungary and Ukraine, its degree of sustainability under various scenarios of forest growth and timber harvest, and the overall consequences of forest decline and climate change on future wood supply was made.
Choline and acetylcholine: novel cationic osmolytes in Lactobacillus plantarum.
Kets, E.P.W. ; Nierop Groot, M. ; Galinski, E.A. ; Bont, J.A.M. de - \ 1997
Applied Microbiology and Biotechnology 48 (1997). - ISSN 0175-7598 - p. 94 - 98.
Mannitol, a novel compatible solute in Pseudomonas putida S12
Kets, E.P.W. ; Galinski, E.A. ; Wit, M. de; Bont, J.A.M. de; Heipieper, H.J. - \ 1996
Journal of Bacteriology 178 (1996). - ISSN 0021-9193 - p. 6665 - 6670.
Mannitol, a novel bacterial compatible solute in Pseudomonas putida S12.
Kets, E.P.W. ; Galinski, E.A. ; Wit, M. de; Bont, J.A.M. de; Heipieper, H.J. - \ 1996
Journal of Bacteriology 178 (1996). - ISSN 0021-9193 - p. 6665 - 6670.
Carnitine: a novel compatible solute in Lactobacillus plantarum accumulated during osmotic stress.
Kets, E.P.W. ; Galinski, E.A. ; Bont, J.A.M. de - \ 1995
In: Abstract Int. Dairy Lactic Acid Bacteria Conf. Palmerston North, New Zealand (1995) 7.16. Ook: Abstract EC Conf. on Improvement of lactic acid bacteria for traditional and novel applications in biotechnology. Cork, Ireland (1995) pI-17
Carnitine: a novel compatible solute in Lactobacillus plantarum.
Kets, E.P.W. ; Galinski, E.A. ; Bont, J.A.M. de - \ 1994
Archives of Microbiology 162 (1994). - ISSN 0302-8933 - p. 243 - 248.
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