Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

    Records 1 - 20 / 235

    • help
    • print

      Print search results

    • export

      Export search results

    Check title to add to marked list
    Between biodiversity conservation and sustainable forest management – A multidisciplinary assessment of the emblematic Białowieża Forest case
    Blicharska, M. ; Angelstam, P. ; Giessen, L. ; Hilszczański, J. ; Hermanowicz, E. ; Holeksa, J. ; Jacobsen, J.B. ; Jaroszewicz, B. ; Konczal, A. ; Konieczny, A. ; Mikusiński, G. ; Mirek, Z. ; Mohren, F. ; Muys, B. ; Niedziałkowski, K. ; Sotirov, M. ; Stereńczak, K. ; Szwagrzyk, J. ; Winder, G.M. ; Witkowski, Z. ; Zaplata, R. ; Winkel, G. - \ 2020
    Biological Conservation 248 (2020). - ISSN 0006-3207
    Białowieża Forest - Biodiversity conservation - Conservation conflict - Forest management - Interdisciplinarity - Land use conflict - Sustainable land use

    The tension between biodiversity conservation and multipurpose forest management may lead to conflicts. An internationally prominent example is the Białowieża Forest Massif (BFM), an extensive forest complex with high levels of naturalness. We apply a systematic, multidisciplinary assessment process to review empirical evidence on different dimensions of the BFM conflict. While there is broad consensus that this forest massif is an exceptional place worth conserving and that a way forward is a zonation system combining conservation with management, exactly how this should be done has yet to be agreed upon. Our assessment shows that the key reasons for the BFM controversy go beyond the availability of knowledge on the ecological status of the BFM and include: 1) evidence stemming from different sources, which is often contradictory and prone to different interpretations; 2) knowledge gaps, particularly with regard to socio-economic drivers and beneficiaries as well as uncertainties about future trends; 3) fundamentally different values and priorities among stakeholder groups, resulting in power struggles, and an overall lack of trust. We conclude that evidence-based knowledge alone is insufficient to cope with complex conservation conflicts. While more evidence may help assess the consequences of decisions, the actual management decisions depend on different actors' worldviews, which are rooted in their professional identities and power, and their political and legal realities. This calls for conflict management through a well-organized participatory process organized and supervised by a body deemed legitimate by the groups involved.

    Methods to assess the effect of meat processing on viability of Toxoplasma gondii: towards replacement of mouse bioassay by in vitro testing
    Opsteegh, M. ; Dam-Deisz, C. ; Boer, Paulo de; Decraeye, S. ; Faré, Andrea ; Hengeveld, P. ; Luiten, R. ; Schares, Gereon ; Smits, C.B. ; Verhaegen, Bavo ; Verkleij, T.J. ; Giessen, Joke van der; Wisselink, Henk J. - \ 2020
    International Journal for Parasitology 50 (2020)5. - ISSN 0020-7519 - p. 357 - 369.
    Consumption of meat containing viable tissue cysts is considered one of the main sources of human infection with Toxoplasma gondii. In contrast to fresh meat, raw meat products usually undergo processing, including salting and mixing with other additives such as sodium acetate and sodium lactate, which affects the viability of T. gondii. However, the experiments described in the literature are not always performed in line with the current processing methods applied in industry. It was our goal to study the effect of salting and additives according to the recipes used by industrial producers. Mouse or cat bioassay is the ‘gold standard’ to demonstrate the presence of viable T. gondii. However, it is costly, time consuming and for ethical reasons not preferred for large-scale studies. Therefore, we first aimed to develop an alternative for mouse bioassay that can be used to determine the effect of processing on the viability of T. gondii tissue cysts. The assays studied were (i) a cell culture method to determine the parasite’s ability to multiply, and (ii) a propidium monoazide (PMA) dye-based assay to selectively detect DNA from intact parasites. Processing experiments were performed with minced meat incubated for 20 h with low concentrations of NaCl, sodium lactate and sodium acetate. NaCl appeared to be the most effective ingredient with only one or two out of eight mice infected after inoculation with pepsin-digest of portions processed with 1.0, 1.2 and 1.6% NaCl. Results of preliminary experiments with the PMA-based method were inconsistent and did not sufficiently discriminate between live and dead parasites. In contrast, the cell culture method showed promising results, but further optimization is needed before it can replace or reduce the number of mouse bioassays needed. In future, standardised in vitro methods are necessary to allow more extensive testing of product-specific processing methods, thereby providing a better indication of the risk of T. gondii infection for consumers.
    Effect of meat processing on viability of Toxoplasma gondii: Towards replacement of mouse bioassay by in vitro testing
    Opsteegh, M. ; Dam-Deisz, C. ; Boer, P. de; Fare, A. ; Hengeveld, P. ; Luiten, R. ; Smits, C.B. ; Verkleij, T. ; Giessen, Judith van der; Wisselink, H.J. - \ 2019
    Felines are the definitive hosts of T. gondii and primary infection results in fecal shedding of infectious oocysts. Infected intermediate hosts will develop tissue cysts, which are infective to both cats and intermediate hosts. Meat containing viable tissue cysts is considered one of the main sources of human infection. In contrast to fresh meat, raw meat products usually undergo processing, including salting and mixing in additives such as acetate and lactate, which affects the viability of T. gondii. However, the experiments currently described in literature, are not always performed in line with the processing methods applied in industry. Therefore we aimed to study the effect of salting and additives according to the recipes used by commercial producers. Mouse or cat bioassay is the gold standard to demonstrate the presence of viable T. gondii. However, it is costly, time consuming and for ethical reasons not preferred for large-scale studies. Therefore, our second aim was to develop an alternative for mouse bioassay that can be used to determine the effect of processing on the viability of T. gondii tissue cysts. We focused on a tissue culture method to determine the parasite's ability to multiply, and a PMA-based assay to selectively detect DNA from live cells. Results with the PMA-based method were inconsistent and did not sufficiently discriminate between live and dead parasites. The tissue culture method showed promising results, but further optimization is needed before it can replace or reduce the number of mouse bioassays needed. Small scale experiments with minced meat incubated for 20h with low concentrations of salt, lactate and acetate showed a large but incomplete reduction of the number of infected mice. In future, in vitro methods are needed to allow more extensive testing of product-specific processing methods, thereby providing a better indication of the risk of T. gondii infection for consumers.
    A risk based surveillance programme for Toxoplasma gondii in pigs using a combination of farm auditing and serological screening
    Wisselink, H.J. ; Swanenburg, M. ; Gonzales Rojas, Jose ; Asseldonk, M.A.P.M. van; Wagenberg, Coen van; Giessen, J. van der; Meerburg, B.G. ; Krijger, Inge ; Eppink, D.M. ; Bouwknegt, M. ; Oorburg, D. - \ 2019
    Toxoplasma gondii is recognized as one of the major foodborne pathogens with a high human disease burden. In the Netherlands, pork contributes to about 11 % of the meatborne T. gondii infections. To control T. gondii infections in pigs, EFSA has advised to perform serological testing of pigs and audits of pig farms on risk factors for T. gondii infection. In the Netherlands, a program was started to translate the EFSA advice into a practical risk based surveillance system. In first instance, a large scale serological monitoring of fattening pigs was started and seroprevalence over time was determined. Next, the association between within-herd seroprevalence and risk factors for T. gondii on fattening pig farms in the Netherlands was determined. For this, a questionnaire for auditing farms for the presence of risk factors of T. gondii was developed and used on 25 case and 50 control farms. Results show that there is a significant association between seroprevalence and risk factors as cats present on farms, use of unheated feed products and feeding wet feed. Moreover, on-farm presence of rats and mice also increases Toxoplasma transmission risks. Subsequently, a study was started on farms to quantify the effectiveness of interventions on farms. A cross-over clinical trial was set up in which case farms were their own control and the cross-over moment is the implementation of interventions on risk factors to change farm management. Farms with a high within-herd seroprevalence were followed for at least during a year and monitored periodically for seroprevalence and implementation of interventions to eventually reduce the disease burden. The break-even point was calculated for which the intervention cost at fattening pig farms equal averted human disease burden and averted cost-of-illness minus cost of the surveillance program. The results shows favourable economic perspectives for interventions to control pig meat-born transmission of T. gondii.
    Identification of potential risk factors for Toxoplasma gondii in fattening pigs in the Netherlands using a Bayesian approach
    Eppink, D.M. ; Bouwknegt, M. ; Oorburg, D. ; Urlings, H.A.P. ; Asseldonk, Marcel van; Wagenberg, Coen van; Krijger, Inge ; Giessen, J.W.P. van der; Swanenburg, M. ; Wisselink, H.J. - \ 2019
    Introduction
    Toxoplasma gondii is a relevant foodborne pathogen,it is estimated that up to one third of the worldpopulation has been exposed to the parasite (Tenteret al. 2000). In the Netherlands toxoplasmosis rankssecond on a list of prioritized emerging zoonosis(Havelaar et al. 2010) and also second in disease burden among 14 foodborne diseases (Mangen et al.2017). Data suggest that ingesting improperly cooked meat containing T. gondii is one of the major sources of infection in Europe and North America (Crotta et al. 2017; Guo et al. 2015). The contribution of pork to meatborne T. gondii infections is estimated to be11 % in the Netherlands (Opsteegh 2011) and is seen as an important possible source of human T. gondii infections (Foroutan et al. 2019). The European Food Safety Authority (EFSA) advised to perform serological testing of pigs and on farm audits on risk factors (EFSA 2011). To that end, a serological monitoring program was developed in a slaughterhouse in the Netherlands. In this study, the objective is to determine the association between within herd seroprevalence, corrected for misclassification of samples through Bayesian analyses, and risk factors for T. gondii on fattening pig farms in TheNetherlands.
    Materials and MethodsFrom 2015 to 2018, HACCP based audits were performedon 75 fattening pig farms in The Netherlands to identify the presence of potential T. gondii risk factors. All farms were conventional pig farms, with 15 farms being farrow to finish. As overall seroprevalence of T. gondii in pigs in the Netherlands is low, estimated at 5 % (1-12 % 95 % CI) by Foroutanet al. 2019, approached farms were chosen with the knowledge of previous serology data. In this way there would be farms with positive serum samples and farms without them included in the study. The audits were based on an updated version of the questionnaire from Mul et al. (2015) and covered the following topics: outdoor access, farm biosecurity, rodent control, presence of cats, feed and watersupply. In addition, serum samples (n=6272) from fattening pigs were obtained at slaughter throughout the year before the audit on the farm was performed. These samples were used for antibody testing bya PrioCHECK™ Toxoplasma Antibody ELISA. Data were analysed using Bayesian statistics, with the within farm T. gondii prevalence as dependent variable and potential risk factors as independent variables. As always with serology, misclassification due to false-positive or false-negative results can occur. Statistical methods have been developed to account for such misclassification, based on frequentistic as well as Bayesian approaches (Hui & Walter 1980; Josephet al. 1995). First, all independent variables wereanalysed in a univariate logistic model, and variables with a probability ≤0.25 that zero is included in the 95 % interval were analysed in a multivariable model. The multivariate logistic model was fitted using backward elimination until all remaining variables showed a probability ≤0.05 that zero is included in the 95 % interval. Two-way interaction terms were evaluated similarly to the main variables regarding statistical significance.
    ResultsDescriptive results showed that 50 out of the 75 farms had 1 or more positive serum samplein the year before the audit was performed. In total 438 samples were positive out of the 6272 samples. Final Bayesian analyses are currently being conducted. However, preliminary results from data analysis using frequentistic logistic multivariate regression identified two significant risk factors: the accessibility of pig feed for cats and theprovision of well water as drinking water for the pigs (Table 1).
    Discussion and ConclusionsThe use of serological testing seems to be a valuable guide and monitoring tool for the control of T.gondii in pork production. In a preliminary analysis, a higher within-herd T. gondii seroprevalence on fattening pig farms in the Netherlands was associated with the accessibility of pig feed for cats and the provision of well water as drinking water for the pigs. Improvements in farm management on fattening pig farms will likely contribute to reduction of the human disease burden and is presently studied.
    The relationship between the presence of antibodies and direct detection of Toxoplasma gondii in slaughtered calves and cattle in four European countries
    Opsteegh, M. ; Spano, F. ; Aubert, D. ; Balea, A. ; Burrells, A. ; Cherchi, S. ; Cornelissen, J.B.W.J. ; Dam-Deisz, C. ; Guitian, J. ; Györke, A. ; Innes, E.A. ; Katzer, F. ; Limon, G. ; Possenti, A. ; Pozio, E. ; Schares, G. ; Villena, I. ; Wisselink, H.J. ; Giessen, J.W.B. van der - \ 2019
    International Journal for Parasitology 49 (2019)7. - ISSN 0020-7519 - p. 515 - 522.
    Cattle - Detection - Mouse bioassay - PCR - Serology - Toxoplasma gondii

    In cattle, antibodies to Toxoplasma gondii infection are frequently detected, but evidence for the presence of T. gondii tissue cysts in cattle is limited. To study the concordance between the presence of anti-T. gondii IgG and viable tissue cysts of T. gondii in cattle, serum, liver and diaphragm samples of 167 veal calves and 235 adult cattle were collected in Italy, the Netherlands, Romania and the United Kingdom. Serum samples were tested for anti-T. gondii IgG by the modified agglutination test and p30 immunoblot. Samples from liver were analyzed by mouse bioassay and PCR after trypsin digestion. In addition, all diaphragms of cattle that had tested T. gondii-positive (either in bioassay, by PCR on trypsin-digested liver or serologically by MAT) and a selection of diaphragms from cattle that had tested negative were analyzed by magnetic capture quantitative PCR (MC-PCR). Overall, 13 animals were considered positive by a direct detection method: seven out of 151 (4.6%) by MC-PCR and six out of 385 (1.6%) by bioassay, indicating the presence of viable parasites. As cattle that tested positive in the bioassay tested negative by MC-PCR and vice-versa, these results demonstrate a lack of concordance between the presence of viable parasites in liver and the detection of T. gondii DNA in diaphragm. In addition, the probability to detect T. gondii parasites or DNA in seropositive and seronegative cattle was comparable, demonstrating that serological testing by MAT or p30 immunoblot does not provide information about the presence of T. gondii parasites or DNA in cattle and therefore is not a reliable indicator of the risk for consumers.

    Association between within-herd seroprevalence and risk factors for Toxoplasma gondii in fattening pigs in the Netherlands
    Eppink, D.M. ; Bouwknegt, M. ; Oorburg, D. ; Urlings, H.A.P. ; Asseldonk, Marcel van; Wagenberg, Coen van; Krijger, I.M. ; Giessen, J.W.P. Van der; Swanenburg, M. ; Wisselink, H.J. - \ 2018
    In: Annual General Meeting of the European College of Veterinary Public Health, "Fading of the HACCP after four decades: new trends in VPH for food safety ", Perugia, 17th-19th October 2018. - European College of Veterinary Public Health (ECVPH) - p. 40 - 40.
    Study on progress in implementing the EU Forest Strategy - Evaluation Study
    Wolfslehner, B. ; Aggestam, F. ; Hurmekoski, E. ; Kulikova, E. ; Lindner, Marcus ; Nabuurs, G.J. ; Pettenella, D. ; Pülzl, H. ; Giessen, L. ; Arets, E.J.M.M. ; Hendriks, C.M.A. - \ 2018
    European Forest Institute - ISBN 9789279864896 - 182 p.
    Serological screening of Dutch slaughter pigs to estimate the seroprevalence of Toxoplasma gondii infections at farms
    Swanenburg, M. ; Rojas, J.G. ; Bouwknegt, M. ; Oorburg, D. ; Giessen, Joke van der; Wisselink, H.J. - \ 2018
    In: The 15th international symposium of veterinary epidemiology and economics. - Chang Mai, Thailand : The World Organisation for Animal Health (OIE) - p. 252 - 252.
    Prevalence of Leptospira spp. and Seoul hantavirus in brown rats (Rattus norvegicus) in four regions in the Netherlands, 2011-2015
    Maas, Miriam ; Vries, Ankje De; Reusken, Chantal ; Buijs, Jan ; Goris, Marga ; Hartskeerl, Rudy ; Ahmed, Ahmed ; Tulden, Peter van; Swart, Arno ; Pijnacker, Roan ; Koene, Miriam ; Lundkvist, Åke ; Heyman, Paul ; Rockx, Barry ; Giessen, Joke Van Der - \ 2018
    Infection Ecology and Epidemiology 8 (2018)1. - ISSN 2000-8686
    epidemiology - hantavirus - Leptospirosis - prevalence - Rattus norvegicus - Seoul virus

    Background: Brown rats (Rattus norvegicus) may carry pathogens that can be a risk for public health. Brown rats in the Netherlands were tested for the zoonotic pathogens Leptospira spp. and Seoul hantavirus (SEOV), in order to obtain insight in their prevalence. Methods and results: Cross-sectional studies were performed at four locations from 2011 to 2015. The rats were tested for Leptospira spp. using real-time PCR and/or culture resulting in a prevalence ranging between 33–57%. Testing for SEOV was done through an adapted human Seoul hantavirus ELISA and real-time RT-PCR. Although at several locations the ELISA indicated presence of SEOV antibodies, none could be confirmed by focus reduction neutralization testing. Conclusion: The results indicate a widespread presence of Leptospira spp. in brown rats in the Netherlands, including areas with a low leptospirosis incidence in humans. No evidence for circulation of SEOV was found in this study.

    Brucella pinnipedialis in grey seals (Halichoerus grypus) and harbor seals (Phoca vitulina) in the Netherlands
    Kroese, Michiel V. ; Beckers, Lisa ; Bisselink, Yvette J.W.M. ; Brasseur, Sophie ; Tulden, Peter W. van; Koene, Miriam G.J. ; Roest, Hendrik I.J. ; Ruuls, Robin C. ; Backer, Jantien A. ; Ijzer, Jooske ; Giessen, Joke W.B. van der; Willemsen, Peter T.J. - \ 2018
    Journal of Wildlife Diseases 54 (2018)3. - ISSN 0090-3558 - p. 439 - 449.
    Brucella pinnipedialis - Halichoerus grypus - MALDI-TOF MS - Marine mammals - MLST - MLVA-16 - Phoca vitulina - The Netherlands

    Brucellosis is a zoonotic disease with terrestrial or marine wildlife animals as potential reservoirs for the disease in livestock and human populations. The primary aim of this study was to assess the presence of Brucella pinnipedialis in marine mammals living along the Dutch coast and to observe a possible correlation between the presence of B. pinnipedialis and accompanying pathology found in infected animals. The overall prevalence of Brucella spp. antibodies in sera from healthy wild grey seals (Halichoerus grypus; n=11) and harbor seals (Phoca vitulina; n=40), collected between 2007 and 2013 ranged from 25% to 43%. Additionally, tissue samples of harbor seals collected along the Dutch shores between 2009 and 2012, were tested for the presence of Brucella spp. In total, 77% (30/ 39) seals were found to be positive for Brucella by IS711 real-time PCR in one or more tissue samples, including pulmonary nematodes. Viable Brucella was cultured from 40% (12/30) real-time PCR-positive seals, and was isolated from liver, lung, pulmonary lymph node, pulmonary nematode, or spleen, but not from any PCR-negative seals. Tissue samples from lung and pulmonary lymph nodes were the main source of viable Brucella bacteria. All isolates were typed as B. pinnipedialis by multiple-locus variable number of tandem repeats analysis-16 clustering and matrix-assisted laser desorption ionization-time of flight mass spectrometry, and of sequence type ST25 by multilocus sequence typing analysis. No correlation was observed between Brucella infection and pathology. This report displays the isolation and identification of B. pinnipedialis in marine mammals in the Dutch part of the Atlantic Ocean.

    A cross sectional study on Dutch layer farms to investigate the prevalence and potential risk factors for different Chlamydia species
    Heijne, Marloes ; Goot, Jeanet A. van der; Fijten, Helmi ; Giessen, Joke W. van der; Kuijt, Eric ; Maassen, Catharina B.M. ; Roon, Annika van; Wit, Ben ; Koets, Ad P. ; Roest, Hendrik I.J. - \ 2018
    PLoS ONE 13 (2018)1. - ISSN 1932-6203
    In poultry several Chlamydia species have been detected, but Chlamydia psittaci and Chlamydia gallinacea appear to be most prevalent and important. Chlamydia psittaci is a well-known zoonosis and is considered to be a pathogen of poultry. Chlamydia gallinacea has been described more recently. Its avian pathogenicity and zoonotic potential have to be further elucidated. Within the Netherlands no data were available on the presence of Chlamydia on poultry farms. As part of a surveillance programme for zoonotic pathogens in farm animals, we investigated pooled faecal samples from 151 randomly selected layer farms. On a voluntary base, 69 farmers, family members or farm workers from these 151 farms submitted a throat swab. All samples were tested with a generic 23S Chlamydiaceae PCR followed by a species specific PCR for C. avium, C. gallinacea and C. psittaci. C. avium and psittaci DNA was not detected at any of the farms. At 71 farms the positive result could be confirmed as C. gallinacea. Variables significantly associated with the presence of C. gallinacea in a final multivariable model were ‘age of hens,’ ‘use of bedding material’ and ‘the presence of horses.’ The presence of C. gallinacea was associated with neither clinical signs, varying from respiratory symptoms, nasal and ocular discharges to diarrhoea, nor with a higher mortality rate the day before the visit. All throat swabs from farmers, family members or farm workers tested negative for Chlamydia DNA, giving no further indication for possible bird-to-human (or human-to-bird) transmission.
    Using serological monitoring, internet-based feedback and on-farm auditing to improve Toxoplasma gondii control at Dutch pig farms
    Oorburg, D. ; Eppink, Dorien ; Heijltjes, Janneke ; Bouwknegt, Martijn ; Urlings, Bert ; Giessen, Joke van der; Krijger, Inge ; Mul, Monique ; Swanenburg, M. ; Wisselink, H.J. - \ 2017
    In: 12th International Symposium on the Epidemiology and Control of Biological,Chemical and Physical Hazards in Pigs and Pork - Proceedings Book, Foz doIguaçu, august 21-24, 2017. - Embrapa - p. 201 - 201.
    Toxoplasma gondii is a relevant foodborne pathogen due to its human disease burden. In the Netherlands, pork is estimated to contribute to 11% of the meatborne T. gondii infections. The European Food Safety Authority advised to perform serological testing of pigs and on farm audits on risk factors for T. gondii infection.
    One health-samenwerking in de aanpak van psittacose
    Heijne, M. ; Hogerwerf, Lenny ; Dijkstra, Frederika ; Goot, J.A. van der; Heddema, Edou ; Kroneman, A. ; Notermans, D. ; Pannekoek, Yvonne ; Rosa, Mauro de; Wierik, M. te; Giessen, Joke van der; Roest, H.I.J. ; Hoek, Wim van der - \ 2017
    Nederlands Tijdschrift voor Medische Microbiologie 25 (2017)2. - ISSN 0929-0176 - p. 43 - 48.
    Psittacose is een zoönose veroorzaakt door de bacterie Chlamydia psittaci. In Nederland is psittacose bij mensen een meldingsplichtige en bij vogels (uitgezonderd pluimvee) een aangifteplichtige ziekte. Van 2011 tot 2015 werden 41 tot 70 humane meldingen per jaar gedaan, maar dit is een onderschatting. Het aantal pneumonieën veroorzaakt door C. psittaci wordt op 1500 per jaar geschat. In 2014 is het One Health-project Plat4m-2bt-Psittacosis gestart. In dit project wordt een onlineplatform ontwikkeld om uitwisseling van data te faciliteren en de samenwerking tussen het humane en veterinaire veld te verbeteren. Onderdeel van het project is het beter in kaart brengen van de aviaire bronnen van psittacose. Recent wordt, naast papegaaiachtigen en duiven, ook pluimvee als mogelijke bron van humane C. psittaci-infecties gezien. Op basis van de jaarlijkse humane psittacosemeldingen is, ook in Nederland, een link gelegd tussen psittacose en de aanwezigheid van kippenslachterijen en eendenbedrijven. Bij een Nederlandse studie op ongeveer 150 leghennenbedrijven is echter geen C. psittaci-DNA aangetoond, maar bij ongeveer de helft van de bedrijven wel een relatief nieuwe Chlamydia-soort: C. gallinacea. Het zoönotisch potentieel van C. gallinacea is nog onduidelijk. Met de ontwikkeling en toepassing van het onlineplatform wil het Platform-2bt-Psittacosis een voorbeeld vormen voor een structurele aanpak van de bestrijding van zoönosen.
    Environmental surveillance during an outbreak of tularaemia in hares, the Netherlands, 2015
    Janse, Ingmar ; Maas, M. ; Rijks, J.M. ; Koene, M. ; Plaats, R.Q. van der; Engelsma, M. ; Tas, P.W.L. ; Braks, M. ; Stroo, A. ; Notermans, D.W. ; Vries, M.C. de; Reubsaet, F.A.G. ; Fanoy, E. ; Swaan, C.M. ; Kik, M.J. ; Ijzer, J. ; Jaarsma, R.I. ; Wieren, S. van; Roda Husman, A.M. de; Passel, M. van; Roest, H. ; Giessen, J. van der - \ 2017
    Eurosurveillance 22 (2017)35. - ISSN 1025-496X
    Tularaemia, a disease caused by the bacterium Francisella tularensis, is a re-emerging zoonosis in the Netherlands. After sporadic human and hare cases occurred in the period 2011 to 2014, a cluster of F. tularensis-infected hares was recognised in a region in the north of the Netherlands from February to May 2015. No human cases were identified, including after active case finding. Presence of F. tularensis was investigated in potential reservoirs and transmission routes, including common voles, arthropod vectors and surface waters. F. tularensis was not detected in common voles, mosquito larvae or adults, tabanids or ticks. However, the bacterium was detected in water and sediment samples collected in a limited geographical area where infected hares had also been found. These results demonstrate that water monitoring could provide valuable information regarding F. tularensis spread and persistence, and should be used in addition to disease surveillance in wildlife.
    Knaagdieren & Zoönosen: Toxoplasma gondii
    Opsteegh, M. ; Giessen, J.W.B. van der; Meerburg, B.G. - \ 2016
    In: Staat van Zoönosen 2016 Bilthoven : RIVM (RIVM rapport 2017-0142) - p. 84 - 84.
    Identification of pig farms with a high risk for Toxoplasma gondii using a combination of serological monitoring and auditing
    Wisselink, H.J. ; Swanenburg, M. ; Gonzales Rojas, Jose ; Asseldonk, Marcel van; Wagenberg, Coen van; Giessen, J.W.B. van der; Mul, M.F. ; Klein Koerkamp, M. ; Oorburg, D. - \ 2016
    In: 4th EAVLD Congress of the European Association of Veterinary Laboratory Diagnosticians. - Prague : European Association of Veterinary Laboratory Diagnosticians (4th EAVLD Congress of the European Association of Veterinary Laboratory Diagnosticians ) - p. 42 - 42.
    Implementing wildlife disease surveillance in the Netherlands, a One Health approach
    Maas, M. ; Gröne, A. ; Kuiken, T. ; Schaik, G. Van; Roest, H.I.J. ; Giessen, J.W.B. Van Der - \ 2016
    Revue scientifique et technique / Office International des Epizooties 35 (2016)3. - ISSN 0253-1933 - p. 863 - 874.
    One health - Overview - Public health - Surveillance - The Netherlands - Veterinary health - Wildlife disease
    The surveillance of (emerging) wildlife diseases can provide important, objective evidence of the circulation of pathogens of interest for veterinary and/or public health. The involvement of multiple research institutions in wildlife disease surveillance can ensure the best use of existing knowledge and expertise, but can also complicate or add challenges to the integration of wildlife disease surveillance components into a national programme. Documenting the existing efforts in a country's surveillance of wildlife diseases, including the institutes in which it takes place, provides a basis for policy-makers and authorities to identify gaps and priorities in their current surveillance programmes. This paper describes the wildlife disease surveillance activities taking place in the Netherlands. The authors recommend that, in addition to funding these current activities, surveillance resources should be allocated with the flexibility to allow for additional targeted surveillance, to detect and adequately respond to newly introduced or emerging pathogens. Similar structured overviews of wildlife disease surveillance in other countries would be very useful to facilitate international collaboration.
    Tularemie in Nederland, terug van weggeweest?
    Pijnacker, Roan ; Koene, M.G.J. ; Rijks, J.M. ; Swaan, C.M. ; Maas, M. ; Rosa, M. de; Broens, E.M. ; Fanoy, E. ; Vries, M.C. de; Notermans, D.W. ; Tas, P. v.d.; Gröne, A. ; Roest, H.I.J. ; Pelt, W. van; Giessen, J. van der - \ 2016
    Nederlands Tijdschrift voor Medische Microbiologie 24 (2016)2. - ISSN 0929-0176 - p. 65 - 68.
    Tularemie is een bacteriële zoönose veroorzaakt door Francisella tularensis. De ziekte kent verschillende verschijningsvormen, dierlijke reservoirs en transmissieroutes. De bacterie kan zich maandenlang handhaven buiten
    een dierlijke gastheer. Na decennialange afwezigheid in Nederland is tularemie vanaf 2011 incidenteel aangetoond bij zowel hazen als mensen. Begin 2015 was er een opvallend hoge sterfte onder hazen in de omgeving van Akkrum in Friesland. In dit gebied werd bij 11 hazen tularemie aangetoond. In dit artikel bespreken we de achtergrond en de huidige situatie van tularemie in Nederland
    Coronary microvascular dysfunction after long-term diabetes and hypercholesterolemia
    Sorop, Oana ; Heuvel, Mieke van den; Ditzhuijzen, Nienke S. van; Beer, Vincent J. de; Heinonen, Ilkka ; Duin, Richard W.B. van; Zhou, Zhichao ; Koopmans, Sietse J. ; Merkus, Daphne ; Giessen, Wim J. van der - \ 2016
    American Journal of Physiology : Heart and Circulatory Physiology 311 (2016)6. - ISSN 0363-6135 - p. H1339 - H1351.
    Coronary microvascular dysfunction - Diabetes - Endothelin-1 - Hypercholesterolemia - Swine

    Coronary microvascular dysfunction (CMD) has been proposed as an important component of diabetes mellitus (DM)-and hypercholesterolemia-associated coronary artery disease (CAD). Previously we observed that 2.5 mo of DM and high-fat diet (HFD) in swine blunted brady-kinin (BK)-induced vasodilation and attenuated endothelin (ET)-1-mediated vasoconstriction. Here we studied the progression of CMD after 15 mo in the same animal model of CAD. Ten male swine were fed a HFD in the absence (HFD, n = 5) or presence of streptozotocin-induced DM (DM + HFD. n = 5). Responses of small (~300-μm-diameter) coronary arteries to BK. ET-1. and the nitric oxide (NO) donor 5-nitroso-N-acetylpenicillamine were examined in vitro and compared with those of healthy (Normal) swine (n = 12). Blood glucose was elevated in DM + HFD (17.6 ± 4.5 mmol/1) compared with HFD (5.1 ± 0.4 mmol/1) and Normal (5.8 ± 0.6 mmol/1) swine, while cholesterol was markedly elevated in DM + HFD (16.8 ± 1.7 mmol/1) and HFD (18.1 ±2.6 mmol/1) compared with Normal (2.1 ± 0.2 mmol/1) swine (all P <0.05). Small coronary arteries showed early atherosclerotic plaques in HFD and DM + HFD swine. Surprisingly. DM + HFD and HFD swine maintained BK responsiveness compared with Normal swine due to an increase in NO availability relative to endothelium-derived hyperpolarizing factors. However, ET-1 responsiveness was greater in HFD and DM + HFD than Normal swine (both P <0.05), resulting mainly from ΕΤ» receptor-mediated vasoconstriction. Moreover, the calculated vascular stiffness coefficient was higher in DM + HFD and HFD than Normal swine (both P <0.05). In conclusion. 15 mo of DM + HFD, as well as HFD alone, resulted in CMD. Although the overall vasodilation to BK was unperturbed, the relative contributions of NO and endothelium-de-rived hyperpolarizing factor pathways were altered. Moreover, the vasoconstrictor response to ET-1 was enhanced, involving the ΕΤB receptors. In conjunction with our previous study, these findings highlight the time dependence of the phenotype of CMD.

    Check title to add to marked list
    << previous | next >>

    Show 20 50 100 records per page

     
    Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.