Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Biological and anthropological drivers for emerging zoonoses from an interdisciplinary perspective
    Swanenburg, M. ; Lauwere, C.C. de; Roest, H.I.J. ; Poel, W.H.M. van der; Boer, F. de; Vaandrager, L. ; Bergevoet, R.H.M. ; Hogeveen, H. ; Petie, R. ; Vos-de Jong, C.J. de - \ 2018
    Root branching toward water involves posttranslational modification of transcription factor ARF7
    Orosa-Puente, Beatriz ; Leftley, Nicola ; Wangenheim, Daniel von; Banda, Jason ; Srivastava, Anjil K. ; Hill, Kristine ; Truskina, Jekaterina ; Bhosale, Rahul ; Morris, Emily ; Srivastava, Moumita ; Kümpers, Britta ; Goh, Tatsuaki ; Fukaki, Hidehiro ; Vermeer, Joop E.M. ; Vernoux, Teva ; Dinneny, José R. ; French, Andrew P. ; Bishopp, Anthony ; Sadanandom, Ari ; Bennett, Malcolm J. - \ 2018
    Science 362 (2018)6421. - ISSN 0036-8075 - p. 1407 - 1410.

    Plants adapt to heterogeneous soil conditions by altering their root architecture. For example, roots branch when in contact with water by using the hydropatterning response. We report that hydropatterning is dependent on auxin response factor ARF7. This transcription factor induces asymmetric expression of its target gene LBD16 in lateral root founder cells. This differential expression pattern is regulated by posttranslational modification of ARF7 with the small ubiquitin-like modifier (SUMO) protein. SUMOylation negatively regulates ARF7 DNA binding activity. ARF7 SUMOylation is required to recruit the Aux/IAA (indole-3-acetic acid) repressor protein IAA3. Blocking ARF7 SUMOylation disrupts IAA3 recruitment and hydropatterning. We conclude that SUMO-dependent regulation of auxin response controls root branching pattern in response to water availability.

    Biological and anthropological drivers for emerging zoonoses from an interdisciplinary perspective
    Swanenburg, M. ; Lauwere, C.C. de; Roest, H.I.J. ; Poel, W.H.M. van der; Boer, F. De; Vaandrager, L. ; Bergevoet, R.H.M. ; Hogeveen, H. ; Vos-de Jong, C.J. de - \ 2018
    Correlation of instrumental texture properties from textural profile analysis (TPA) with eating behaviours and macronutrient composition for a wide range of solid foods
    Wee, May Sui Mei ; Goh, Ai Ting ; Stieger, Markus ; Forde, Ciarán G. - \ 2018
    Food & Function 9 (2018)10. - ISSN 2042-6496 - p. 5301 - 5312.

    Faster eating rates have previously been associated with higher ad libitum energy intakes, and several studies have manipulated eating rates and intake by changing food textures. Food texture based changes to slow eating rates can produce reductions in energy intake without affecting post-meal satisfaction or re-bound hunger. However, an understanding of how specific food textures and instrumental texture properties influence oral processing behaviour remains limited. The current study sought to establish relationships between objective measures of oral processing behaviour (i.e. number of bites, average bite size, total chews, chews per bite, oro-sensory exposure time and eating rate) and instrumental measures of a food texture including hardness, adhesiveness, springiness, cohesiveness, chewiness, resilience and modulus. Across two studies, behavioural coding analysis was completed on video-recordings of participants consuming fixed portions of a wide range of different solid foods (n = 59) to derive objective measures of oral processing behaviours. These measures were correlated with instrumental Textural Profile Analysis (TPA) for the same set of foods. Significant correlations (p < 0.05) were found between oral processing parameters and texture properties (i.e. springiness, cohesiveness, chewiness and resilience). No significant correlations were found between hardness and modulus and oral processing parameters. Protein content of the food was associated with springiness and chewiness, which may help to further reduce eating rates. In terms of the ‘breakdown path model', hardness and modulus might represent degree of initial food structure while springiness, cohesiveness, chewiness and resilience seem to determine how fast the degree of structure is reduced to the swallowing plane. Water content and adhesiveness were associated with level of lubrication that is required before reaching the swallowing plane. The current study highlights opportunities to understand eating rate (g min−1) through the breakdown path model and the potential for specific features of a foods texture to influence rate and extent of energy intake. The correlation between instrumental texture properties and oral processing patterns provides guidance on the parameters that are likely to produce ‘faster' and ‘slower' versions of foods, and suggests how texture modifications could be applied to moderate eating rate and energy intake within meals.

    Ratio of Klebsiella/Bifidobacterium in early life correlates with later development of paediatric allergy
    Low, J.S.Y. ; Soh, S.E. ; Lee, Y.K. ; Kwek, K.Y.C. ; Holbrook, J.D. ; Beek, E.M. Van der; Shek, L.P. ; Goh, A.E.N. ; Teoh, O.H. ; Godfrey, K.M. ; Chong, Y.S. ; Knol, J. ; Lay, C. - \ 2017
    Beneficial Microbes 8 (2017)5. - ISSN 1876-2883 - p. 681 - 695.
    16S rRNA - Atopic dermatitis - Bifidobacterium spp - Eczema - Enterobacteriaceae
    Several studies have reported that intestinal microbial colonisation patterns differ between non-allergic and allergic infants. However, the microbial signature underlying the pathogenesis of allergies remains unclear. We aim to gain insight into the development of the intestinal microbiota of healthy infants and infants who develop allergy in early life, and identify potential microbiota biomarkers of later allergic disease. Using a case-control design in a Chinese sub-cohort of a Singaporean birth cohort (GUSTO), we utilised 16S rRNA gene sequencing to assess intestinal microbial composition and diversity of 21 allergic and 18 healthy infants at 3 weeks, 3 months and 6 months of age, and correlated the microbiota with allergy at ages 18 and 36 months. Pronounced differences in intestinal microbiota composition between allergic and healthy infants were observed at 3 months of age. The intestine of healthy infants was colonised with higher abundance of commensal Bifidobacterium. Conversely, Klebsiella, an opportunistic pathogen, was significantly enriched in the allergic infants. Interestingly, infants with a high Klebsiella/Bifidobacterium (K/B) ratio (above the population median K/B ratio) at age 3 months had an odds ratio of developing allergy by 3 years of age of 9.00 (95% confidence interval 1.46-55.50) compared to those with low K/B ratio. This study demonstrated a relationship between the ratio of genera Klebsiella and Bifidobacterium during early infancy and development of paediatric allergy in childhood. Our study postulates that an elevated K/B ratio in early infancy could be a potential indicator of an increased risk of allergy development. This line of research might enable future intervention strategies in early life to prevent or treat allergy. Our study provides new insights into microbial signatures associated with childhood allergy, in particular, suggests that an elevated K/B ratio could be a potential early-life microbiota biomarker of allergic disease.
    Quiescent center initiation in the Arabidopsis lateral root primordia is dependent on the SCARECROW transcription factor
    Goh, Tatsuaki ; Toyokura, Koichi ; Wells, Darren M. ; Swarup, Kamal ; Yamamoto, Mayuko ; Mimura, Tetsuro ; Weijers, Dolf ; Fukaki, Hidehiro ; Laplaze, Laurent ; Bennett, Malcolm J. ; Guyomarc’h, Soazig - \ 2016
    Development 143 (2016)18. - ISSN 0950-1991 - p. 3363 - 3371.
    3D live imaging - Arabidopsis thaliana - Lateral root organogenesis - Quiescent center - SCARECROW

    Lateral root formation is an important determinant of root system architecture. In Arabidopsis, lateral roots originate from pericycle cells, which undergo a program of morphogenesis to generate a new lateral root meristem. Despite its importance for root meristem organization, the onset of quiescent center (QC) formation during lateral root morphogenesis remains unclear. Here, we used live 3D confocal imaging to monitor cell organization and identity acquisition during lateral root development. Our dynamic observations revealed an early morphogenesis phase and a late meristem formation phase as proposed in the bi-phasic growth model. Establishment of lateral root QCs coincided with this developmental phase transition. QC precursor cells originated from the outer layer of stage II lateral root primordia, within which the SCARECROW (SCR) transcription factor was specifically expressed. Disrupting SCR function abolished periclinal divisions in this lateral root primordia cell layer and perturbed the formation of QC precursor cells. We conclude that de novo QC establishment in lateral root primordia operates via SCR-mediated formative cell division and coincides with the developmental phase transition.

    Sandwich-Architectured Poly(lactic acid)-Graphene Composite Food Packaging Films
    Goh, Kunli ; Heising, Jenneke K. ; Yuan, Yang ; Karahan, Huseyin E. ; Wei, Li ; Zhai, Shengli ; Koh, Jia Xuan ; Htin, Nanda M. ; Zhang, Feimo ; Wang, Rong ; Fane, Anthony G. ; Dekker, Matthijs ; Dehghani, Fariba ; Chen, Yuan - \ 2016
    ACS Applied Materials and Interfaces 8 (2016)15. - ISSN 1944-8244 - p. 9994 - 10004.
    food packaging - graphene oxide - oxygen permeability - poly(lactic acid) - shelf-life simulations - water vapor permeability

    Biodegradable food packaging promises a more sustainable future. Among the many different biopolymers used, poly(lactic acid) (PLA) possesses the good mechanical property and cost-effectiveness necessary of a biodegradable food packaging. However, PLA food packaging suffers from poor water vapor and oxygen barrier properties compared to many petroleum-derived ones. A key challenge is, therefore, to simultaneously enhance both the water vapor and oxygen barrier properties of the PLA food packaging. To address this issue, we design a sandwich-architectured PLA-graphene composite film, which utilizes an impermeable reduced graphene oxide (rGO) as the core barrier and commercial PLA films as the outer protective encapsulation. The synergy between the barrier and the protective encapsulation results in a significant 87.6% reduction in the water vapor permeability. At the same time, the oxygen permeability is reduced by two orders of magnitude when evaluated under both dry and humid conditions. The excellent barrier properties can be attributed to the compact lamellar microstructure and the hydrophobicity of the rGO core barrier. Mechanistic analysis shows that the large rGO lateral dimension and the small interlayer spacing between the rGO sheets have created an extensive and tortuous diffusion pathway, which is up to 1450-times the thickness of the rGO barrier. In addition, the sandwiched architecture has imbued the PLA-rGO composite film with good processability, which increases the manageability of the film and its competency to be tailored. Simulations using the PLA-rGO composite food packaging film for edible oil and potato chips also exhibit at least eight-fold extension in the shelf life of these oxygen and moisture sensitive food products. Overall, these qualities have demonstrated the high potential of a sandwich-architectured PLA-graphene composite film for food packaging applications. (Figure Presented).

    A sensitive epitope-blocking ELISA for the detection of Chikungunya virus-specific antibodies in patients
    Goh, L.Y.H. ; Kam, Y.W. ; Metz, S.W.H. ; Hobson-Peters, J. ; Prow, N.A. ; McCarthy, S. ; Smith, D.W. ; Pijlman, G.P. ; Ng, L.F.P. ; Hall, R.A. - \ 2015
    Journal of Virological Methods 222 (2015). - ISSN 0166-0934 - p. 55 - 61.
    west-nile-virus - linked-immunosorbent-assay - valley encephalitis-virus - monoclonal-antibodies - diagnostic-accuracy - universal detection - reunion island - insect cells - ns1 protein - pcr assay
    Chikungunya fever (CHIKF) has re-emerged as an arboviral disease that mimics clinical symptoms of other diseases such as dengue, malaria, as well as other alphavirus-related illnesses leading to problems with definitive diagnosis of the infection. Herein we describe the development and evaluation of a sensitive epitope-blocking ELISA (EB-ELISA) capable of specifically detecting anti-chikungunya virus (CHIKV) antibodies in clinical samples. The assay uses a monoclonal antibody (mAb) that binds an epitope on the E2 protein of CHIKV and does not exhibit cross-reactivity to other related alphaviruses. We also demonstrated the use of recombinant CHIK virus-like particles (VLPs) as a safe alternative antigen to infectious virions in the assay. Based on testing of 60 serum samples from patients in the acute or convalescent phase of CHIKV infection, the EB-ELISA provided us with 100% sensitivity, and exhibited 98.5% specificity when Ross River virus (RRV)- or Barmah Forest virus (BFV)-immune serum samples were included. This assay meets the public health demands of a rapid, robust, sensitive and specific, yet simple assay for specifically diagnosing CHIK-infections in humans.
    Angiopoietin-like 4 Stimulates STAT3-mediated iNOS Expression and Enhances Angiogenesis to Accelerate Wound Healing in Diabetic Mice
    Chong, H.C. ; Goh, C.Q. ; Gounko, N.V. ; Luo, B. ; Wang, X. ; Kersten, A.H. - \ 2014
    Molecular Therapy 22 (2014)9. - ISSN 1525-0016 - p. 1593 - 1604.
    nitric-oxide synthase - solid human tumors - international consensus - growth-factors - foot ulcers - repair - cells - quantification - methodology - mechanisms
    Impaired wound healing is a major source of morbidity in diabetic patients. Poor outcome has, in part, been related to increased inflammation, poor angiogenesis, and deficiencies in extracellular matrix components. Despite the enormous impact of these chronic wounds, effective therapies are lacking. Here, we showed that the topical application of recombinant matricellular protein angiopoietin-like 4 (ANGPTL4) accelerated wound reepithelialization in diabetic mice, in part, by improving angiogenesis. ANGPTL4 expression is markedly elevated upon normal wound injury. In contrast, ANGPTL4 expression remains low throughout the healing period in diabetic wounds. Exogenous ANGPTL4 modulated several regulatory networks involved in cell migration, angiogenesis, and inflammation, as evidenced by an altered gene expression signature. ANGPTL4 influenced the expression profile of endothelial-specific CD31 in diabetic wounds, returning its profile to that observed in wild-type wounds. We showed ANGPTL4-induced nitric oxide production through an integrin/JAK/STAT3-mediated upregulation of inducible nitric oxide synthase (iNOS) expression in wound epithelia, thus revealing a hitherto unknown mechanism by which ANGPTL4 regulated angiogenesis via keratinocyte-to-endothelial-cell communication. These data show that the replacement of ANGPTL4 may be an effective adjunctive or new therapeutic avenue for treating poor healing wounds. The present finding also confirms that therapeutic angiogenesis remains an attractive treatment modality for diabetic wound healing.
    Effective Chikungunya Virus-like Particle Vaccine Produced in Insect Cells
    Metz, S.W.H. ; Gardner, J. ; Geertsema, C. ; Le, T.T. ; Goh, L. ; Vlak, J.M. ; Suhrbier, A. ; Pijlman, G.P. - \ 2013
    PLoS Neglected Tropical Diseases 7 (2013)3. - ISSN 1935-2727
    equine encephalitis-virus - envelope proteins - baculovirus vectors - inactivated vaccine - expression system - dna vaccines - immunogenicity - infection - opportunities - glycosylation
    The emerging arthritogenic, mosquito-borne chikungunya virus (CHIKV) causes severe disease in humans and represents a serious public health threat in countries where Aedes spp mosquitoes are present. This study describes for the first time the successful production of CHIKV virus-like particles (VLPs) in insect cells using recombinant baculoviruses. This well-established expression system is rapidly scalable to volumes required for epidemic responses and proved well suited for processing of CHIKV glycoproteins and production of enveloped VLPs. Herein we show that a single immunization with 1 µg of non-adjuvanted CHIKV VLPs induced high titer neutralizing antibody responses and provided complete protection against viraemia and joint inflammation upon challenge with the Réunion Island CHIKV strain in an adult wild-type mouse model of CHIKV disease. CHIKV VLPs produced in insect cells using recombinant baculoviruses thus represents as a new, safe, non-replicating and effective vaccine candidate against CHIKV infections.
    Rumen degradation of oil palm fronds is improved through pre-digestion with white rot fungi but not through supplementation with yeast or enzymes
    Hassim, H.A. ; Lourenco, M. ; Goh, Y.M. ; Baars, J.J.P. ; Fievez, V. - \ 2012
    Canadian Journal of Animal Science 92 (2012)1. - ISSN 0008-3984 - p. 79 - 87.
    vitro fermentation characteristics - in-vitro - rice straw - chemical-composition - fibrolytic enzymes - wheat-straw - metabolism - degradability - digestibility - culture
    Rumen fermentation kinetics of oil palm fronds (OPF) supplemented or not with enzymes (Hemicell® or Allzyme SSF®) or yeasts (Levucell®SC or Yea-Sacc®) were studied through an in vitro gas production test (96 h) (exp. 1). In exp. 2, enzymes were supplemented to OPF pre-treated during 3 or 9 wk with either one of five white rot fungi strains. Yeasts and enzymes were tested both in active and inactive forms, which revealed the most appropriate set-up to distinguish between the rate of supplements as direct contributors to the fermentation substrate vs. stimulators of the fermentation of the basal substrate. In exp 1, addition of active and inactive Yea-Sacc® increased the apparently rumen degradable carbohydrates (ARDC) by 11%, whereas enzymes did not affect rumen degradability of non-inoculated OPF. Neither yeast nor enzymes influenced the rate of gas production of non-inoculated OPF, except for active Hemicell® at the low dose. In exp. 2, inoculation of OPF with Ceriporiopsis subvermispora for 3 wk and Lentinula edodes for 9 wk increased ARDC, but additional enzyme supplementation did not further improve ARDC or the rate of gas production.
    Angiopoietin-like 4: a decade of research
    Zhu, P. ; Goh, Y.Y. ; Chin, H.F.A. ; Kersten, A.H. ; Tan, N.S. - \ 2012
    Bioscience Reports 32 (2012)3. - ISSN 0144-8463 - p. 211 - 219.
    induced adipose factor - fatty-acids - tgf-beta - anoikis resistance - lipoprotein-lipase - human adipocytes - target gene - protein - angptl4 - expression
    The past decade has seen a rapid development and increasing recognition of ANGPTL4 (angiopoietin-like 4) as a remarkably multifaceted protein that is involved in many metabolic and non-metabolic conditions. ANGPTL4 has been recognised as a central player in various aspects of energy homoeostasis, at least in part, via the inhibitory interaction between the coiled-coil domain of ANGPTL4 and LPL (lipoprotein lipase). The fibrinogen-like domain of ANGPTL4 interacts and activates specific integrins to facilitate wound healing, modulates vascular permeability, and regulates ROS (reactive oxygen species) level to promote tumorigenesis. The present review summarizes these landmark findings about ANGPTL4 and highlights several important implications for future clinical practice. Importantly, these implications have also raised many questions that are in urgent need of further investigations, particularly the transcription regulation of ANGPTL4 expression, and the post-translation cleavage and modifications of ANGPTL4. The research findings over the past decade have laid the foundation for a better mechanistic understanding of the new scientific discoveries on the diverse roles of ANGPTL4.
    Goh, staat het fluitenkruid nú al in bloei?
    Vliet, A.J.H. van - \ 2012
    NRC-Handelsblad (2012). - ISSN 0002-5259
    Angiopoietin-like 4 Interacts with Matrix Proteins to Modulate Wound Healing
    Goh, Y.Y. ; Pal, M. ; Chong, H.C. ; Zhu, P. ; Tan, M.J. ; Punugu, L. ; Tan, C.K. ; Huang, R.L. ; Sze, S.K. ; Yang Tang, M.B. ; Ling Ding, J. ; Kersten, A.H. ; Tan, N.S. - \ 2010
    Journal of Biological Chemistry 285 (2010)43. - ISSN 0021-9258 - p. 32999 - 33009.
    activated receptor-beta/delta - human epidermal-keratinocytes - ppar-beta/delta - transcriptional control - cell-growth - expression - beta - migration - target - repair
    A dynamic cell-matrix interaction is crucial for a rapid cellular response to changes in the environment. Appropriate cell behavior in response to the changing wound environment is required for efficient wound closure. However, the way in which wound keratinocytes modify the wound environment to coordinate with such cellular responses remains less studied. We demonstrated that angiopoietin-like 4 (ANGPTL4) produced by wound keratinocytes coordinates cell-matrix communication. ANGPTL4 interacts with vitronectin and fibronectin in the wound bed, delaying their proteolytic degradation by metalloproteinases. This interaction does not interfere with integrin-matrix protein recognition and directly affects cell-matrix communication by altering the availability of intact matrix proteins. These interactions stimulate integrin- focal adhesion kinase, 14-3-3, and PKC-mediated signaling pathways essential for effective wound healing. The deficiency of ANGPTL4 in mice delays wound re-epithelialization. Further analysis revealed that cell migration was impaired in the ANGPTL4-deficient keratinocytes. Altogether, the findings provide molecular insight into a novel control of wound healing via ANGPTL4-dependent regulation of cell-matrix communication. Given the known role of ANGPTL4 in glucose and lipid homeostasis, it is a prime therapeutic candidate for the treatment of diabetic wounds. It also underscores the importance of cell-matrix communication during angiogenesis and cancer metastasis.
    Angiopoietin-Like 4 Interacts with Integrins ß1 and ß5 to Modulate Keratinocyte Migration
    Goh, Y.Y. ; Pal, M. ; Chong, H.C. ; Zhu, P. ; Tan, M.J. ; Punugu, L. ; Lam, C.R.I. ; Yau, Y.H. ; Tan, C.K. ; Huang, R.L. ; Tan, S. ; Yang Tang, M.B. ; Ling Ding, J. ; Kersten, A.H. ; Tan, N.S. - \ 2010
    American Journal of Pathology 177 (2010)6. - ISSN 0002-9440 - p. 2791 - 2803.
    induced adipose factor - cell-migration - alpha-6-beta-4 integrin - in-vivo - protein - kinase - expression - repair - metastasis - inhibition
    Adipose tissue secretes adipocytokines for energy homeostasis, but recent evidence indicates that some adipocytokines also have a profound local impact on wound healing. Upon skin injury, keratinocytes use various signaling molecules to promote reepithelialization for efficient wound closure. In this study, we identify a novel function of adipocytokine angiopoietin-like 4 (ANGPTL4) in keratinocytes during wound healing through the control of both integrin-mediated signaling and internalization. Using two different in vivo models based on topical immuno-neutralization of ANGPTL4 as well as ablation of the ANGPTL4 gene, we show that ANGPTL4-deficient mice exhibit delayed wound reepithelialization with impaired keratinocyte migration. Human keratinocytes in which endogenous ANGPTL4 expression was suppressed by either siRNA or a neutralizing antibody show impaired migration associated with diminished integrin-mediated signaling. Importantly, we identify integrins ß1 and ß5, but not ß3, as novel binding partners of ANGPTL4. ANGPTL4-bound integrin ß1 activated the FAK-Src-PAK1 signaling pathway, which is important for cell migration. The findings presented herein reveal an unpredicted role of ANGPTL4 during wound healing and demonstrate how ANGPTL4 stimulates intracellular signaling mechanisms to coordinate cellular behavior. Our findings provide insight into a novel cell migration control mechanism and underscore the physiological importance of the modulation of integrin activity in cancer metastasis
    Characterisation of a monoclonal antibody to carp IL-1 beta and the development of a sensitive capture ELISA
    Mathew, J.A. ; Guo, Y.X. ; Goh, K.P. ; Chan, J. ; Verburg-van Kemenade, B.M.L. ; Kwang, J. - \ 2002
    Fish and Shellfish Immunology 2 (2002). - ISSN 1050-4648 - p. 85 - 95.
    A carp IL-1 gene was identified from a subtraction hybridisation technology based cDNA library from activated carp leucocytes. This gene was cloned into pQE vector carrying 6xHis tag and the protein was expressed. Recombinant IL-1 was used to produce hybridomas specific for carp IL-1. Monoclonal antibodies were purified by affinity column and a sandwich ELISA for IL-1 was developed with a detection limit of 10 ng of the recombinant protein. Using the capture ELISA, the presence of native IL-1 in culture supernatant of PHA-stimulated leucocytes from carp was identified, which was confirmed by SDS-PAGE and Western blot. Since IL-1 is known to stimulate proliferation of T & B cells and macrophages, its ability to stimulate proliferation of carp leucocytes was studied using tritiated thymidine. The recombinant protein was found to significantly stimulate proliferation of head kidney and spleen cells from carp
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