Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Bloemen en bollen: onderzoek van houdbaarheid, transport, logistiek en kwaliteit 9 januari 1991
Drent, P.J. ; Schoot, C. van der; Kooten, O. van; Mensink, M.G.J. ; Otma, E.C. ; Harbinson, J. ; Harkema, H. ; Tijskens, L.M.M. ; Woltering, E.J. ; Berg-Somhorst, B.P.M. van de; Vrije, G.J. de; Halaba, J. ; Gorin, N. ; Rudolphij, J.W. ; Pie, K. ; Peppelenbos, H.W. ; Hoogerwerf, A. ; Reinders, M.P. ; Timmermans, A.J.M. - \ 1991
Wageningen : ATO-Agrotechnologie - 33
Extraction and clean-up of free amino acids from freeze - dried corollas of cut 'Sonia' roses prior to analysis by liquid chromatography : correction of data for 100% recovery of added amino acid
Gorin, N. ; Dreise, G. ; Verhagen, W. - \ 1987
Wageningen : Sprenger Instituut (Rapport / Sprenger Instituut no. 2339) - 14
aminozuren - organische verbindingen - sierplanten - planten - amino acids - organic compounds - ornamental plants - plants
Effects of ethylene on protease activity in extracts of petals and ovaries from intact or cut roses CV. Sonia grown hydroponically : a preliminary note
Lukaszewska, A. ; Perez - Zuniga, F.J. ; Gorin, N. - \ 1985
Wageningen : Sprenger Instituut (Rapport / Sprenger Instituut no. 2305) - 6
ethyleen - snijbloemen - houdbaarheid (kwaliteit) - rosaceae - rosa - ethylene - cut flowers - keeping quality
Assay of protein by the method of Lowry, Rosebrough, Farr and Randall after precipitation : determination of suitable incubation time of the reaction mixture
Perez - Zuniga, F.J. ; Lukaszewska, A. ; Gorin, N. - \ 1985
Wageningen : Sprenger Instituut (Report / Sprenger Instituut no. 2302) - 6
analyse - chemie - organische verbindingen - eiwitten - analytische scheikunde - analysis - chemistry - organic compounds - proteins - analytical chemistry
Liquid chromatographic estimation of fructose, glucose and sucrose in fresh onions and in powder obtained by freeze-drying
Gorin, N. ; Spekking, W.T.J. - \ 1983
Wageningen : Sprenger Instituut (Rapport / Sprenger Instituut no. 2232) - 4
allium cepa - chemische samenstelling - hplc - vloeistofchromatografie - monosacchariden - uien - organische verbindingen - vriesdroging - chemical composition - liquid chromatography - monosaccharides - onions - organic compounds - freeze drying
Treatment of carnations with silver thiocyanate as related to longevity
Klop, W. ; Gorin, N. - \ 1983
Wageningen : Sprenger Instituut (Report / Sprenger Instituut no. 2235) - 10
Enzymatische analyse van glucose, fructose en sucrose in poeder van rozeblaadjes : het effect van het oplosmiddel in de resultaten
Gorin, N. ; Spekking, W.T.J. - \ 1980
Wageningen : Sprenger Instituut (Rapport / Sprenger Instituut no. 2105) - 6
chemie - sierplanten - rosaceae - chemistry - ornamental plants
Histochemical detection of aluminium in roses
Zonneveld, H. ; Gorin, N. - \ 1979
Wageningen : Sprenger Instituut (Rapport / Sprenger Instituut no. 2084) - 4
chemie - sierplanten - rosaceae - chemistry - ornamental plants
Beschrijving van de meetmethode
Selection of tomatoes based on colour and ethylene production for preparation of samples to be used in subsequent studies of enzym activities and firmness
Heidema, F.T. ; Honcoop, T. ; Gorin, N. - \ 1979
Wageningen : Springer instituut (Rapport / Sprenger instituut no. 2048) - 13
plantkunde - solanum lycopersicum - tomaten - ethyleenproductie - botany - tomatoes - ethylene production
The antimicrobial effect of onion and garlic extracts
Wit, J.C. de; Notermans, S.H.W. ; Gorin, W. - \ 1979
Antonie van Leeuwenhoek: : Nederlandsch tijdschrift voor hygiëne, microbiologie en serologie 45 (1979). - ISSN 0003-6072 - p. 156 - 158.
Effect of garlic oil or onion oil on toxin production by Cl. botulinum in meat slurry
Wit, J.C. de; Notermans, S.H.W. ; Gorin, W. ; Kampelmacher, E.H. - \ 1979
Journal of Food Protection 42 (1979). - ISSN 0362-028X - p. 222 - 224.
Ethylene production in stored onions (Allium cepa) cv. Hyduro
Gorin, N. ; Honkoop, F. - \ 1978
Wageningen : Sprenger Instituut (Rapport / Sprenger instituut no. 2014) - 12
allium cepa - uien - ethyleenproductie - onions - ethylene production
The permeability of dead plant cells for some enzymes
Gorin, N. - \ 1969
Wageningen University. Promotor(en): C. den Hartog. - Wageningen : Veenman - 93
membranen - transport - cytologie - plantenfysiologie - enzymen - enzymologie - fermentatie - celfysiologie - membranes - transport - cytology - plant physiology - enzymes - enzymology - fermentation - cell physiology

The penetration of α-chymotrypsin and/or pancreatic lipase into dead cells of soybean cotyledons, of yeast and of algae was studied using the enzymic activity as a parameter. In addition a fluorescent antibody technique was applied for the localization of α-chymotrypsin within the soybean cells.

The digestibility of unheated, EDTA-treated substrate was similar to that of the heated substrate. Since EDTA-treatment increases the permeability of the cell wall and cell membrane of the plant cell without denaturing proteins contained in the cells, we concluded that heating affected mainly the barrier formed by the cell wall, thus permitting a better passage of big molecules (enzymes).

Leakage of β-amylase (mol. weight 61,700) from soybean occurred only after treatment with EDTA. This confirmed that EDTA enhances the permeability of the cell wall.

α-Chymotrypsin (mol. weight 24,000) and pancreatic lipase (mol. weight 38,000) penetrated unheated sections, whereas amylopectin (mol. weight 50,000-1,000,000) did not penetrate unheated cotyledons. Apparently the greater dimension of amylopectin compared with α-chymotrypsin and lipase accounts for its lack of entry into unheated soybean cotyledons. However, when we applied the fluorescent antibody technique to localize α-chymotrypsin within the cells from unheated sections, we used antibodies (γ-globulins) having a mol. weight of 150,000. They entered the unheated sections and produced a positive result of the experiment. Consequently the size of the molecules as indicated by the molecular weight, is not the reason why amylopectin did not penetrate the unheated cotyledons. Moreover, this shows that α-chymotrypsin probably opens a way for the entry of globulins. Therefore the entry of proteolytic enzymes is not an inert process. This is conceivable as the enzymes are big molecules with catalytic activity.

Unheated non-EDTA-treated soybean (with plasmodesmata) was slightly penetrated by α-chymotrypsin, whereas unheated non-EDTA-treated yeast and algae (both without plasmodesmata) were not penetrated at all. Furthermore, in soybean material (unheated or heated) α-chymotrypsin enhanced the penetration of lipase; this effect was absent in the case of yeast. The results obtained strongly suggest that plasmodesmata are a way in dead plant cells for penetration of enzymes.

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