- Anna Deneer (1)
- Christian Fleck (2)
- Bettina Greese (1)
- E. Gruys (1)
- C.A.M. Hulskamp-Koch (1)
- Martin Hülskamp (3)
- Benjamin Jaegle (1)
- Maarten Koornneef (1)
- Jessica Magdalena Pietsch (1)
- Mona Mapar (1)
- Eva Maria Willing (1)
- Emma Mathilde Keizer (1)
- C.L. Murphy (1)
- T.A. Niewold (1)
- Louai Rishmawi (1)
- Swen Schellmann (1)
- Korbinian Schneeberger (1)
- Andrea Schrader (1)
- Ilka Schultheiß Araújo (1)
- Lisa Stephan (1)
- P.C.J. Tooten (1)
Genetic and molecular analysis of trichome development in Arabis alpina
Chopra, Divykriti ; Mapar, Mona ; Stephan, Lisa ; Albani, Maria C. ; Deneer, Anna ; Coupland, George ; Willing, Eva Maria ; Schellmann, Swen ; Schneeberger, Korbinian ; Fleck, Christian ; Schrader, Andrea ; Hülskamp, Martin - \ 2019
Proceedings of the National Academy of Sciences of the United States of America 116 (2019)24. - ISSN 0027-8424 - p. 12078 - 12083.
Arabis alpina - Genetic analysis - Trichomes
The genetic and molecular analysis of trichome development in Arabidopsis thaliana has generated a detailed knowledge about the underlying regulatory genes and networks. However, how rapidly these mechanisms diverge during evolution is unknown. To address this problem, we used an unbiased forward genetic approach to identify most genes involved in trichome development in the related crucifer species Arabis alpina. In general, we found most trichome mutant classes known in A. thaliana. We identified orthologous genes of the relevant A. thaliana genes by sequence similarity and synteny and sequenced candidate genes in the A. alpina mutants. While in most cases we found a highly similar gene-phenotype relationship as known from Arabidopsis, there were also striking differences in the regulation of trichome patterning, differentiation, and morphogenesis. Our analysis of trichome patterning suggests that the formation of two classes of trichomes is regulated differentially by the homeodomain transcription factor AaGL2. Moreover, we show that overexpression of the GL3 basic helix–loop–helix transcription factor in A. alpina leads to the opposite phenotype as described in A. thaliana. Mathematical modeling helps to explain how this nonintuitive behavior can be explained by different ratios of GL3 and GL1 in the two species.
Stochastic gene expression in Arabidopsis thaliana
Araújo, Ilka Schultheiß ; Pietsch, Jessica Magdalena ; Keizer, Emma Mathilde ; Greese, Bettina ; Balkunde, Rachappa ; Fleck, Christian ; Hülskamp, Martin - \ 2017
Nature Communications 8 (2017)1. - ISSN 2041-1723
Although plant development is highly reproducible, some stochasticity exists. This developmental stochasticity may be caused by noisy gene expression. Here we analyze the fluctuation of protein expression in Arabidopsis thaliana. Using the photoconvertible KikGR marker, we show that the protein expressions of individual cells fluctuate over time. A dual reporter system was used to study extrinsic and intrinsic noise of marker gene expression. We report that extrinsic noise is higher than intrinsic noise and that extrinsic noise in stomata is clearly lower in comparison to several other tissues/cell types. Finally, we show that cells are coupled with respect to stochastic protein expression in young leaves, hypocotyls and roots but not in mature leaves. Our data indicate that stochasticity of gene expression can vary between tissues/cell types and that it can be coupled in a non-cell-autonomous manner.
Quantitative trait loci controlling leaf venation in Arabidopsis
Rishmawi, Louai ; Bühler, Jonas ; Jaegle, Benjamin ; Hülskamp, Martin ; Koornneef, Maarten - \ 2017
Plant, Cell & Environment 40 (2017)8. - ISSN 0140-7791 - p. 1429 - 1441.
Arabidopsis natural variation - leaf thickness - multiparent populations - photosynthesis - RC12C - vein structure
Leaf veins provide the mechanical support and are responsible for the transport of nutrients and water to the plant. High vein density is a prerequisite for plants to have C4 photosynthesis. We investigated the genetic variation and genetic architecture of leaf venation traits within the species Arabidopsis thaliana using natural variation. Leaf venation traits, including leaf vein density (LVD) were analysed in 66 worldwide accessions and 399 lines of the multi-parent advanced generation intercross population. It was shown that there is no correlation between LVD and photosynthesis parameters within A. thaliana. Association mapping was performed for LVD and identified 16 and 17 putative quantitative trait loci (QTLs) in the multi-parent advanced generation intercross and worldwide sets, respectively. There was no overlap between the identified QTLs suggesting that many genes can affect the traits. In addition, linkage mapping was performed using two biparental recombinant inbred line populations. Combining linkage and association mapping revealed seven candidate genes. For one of the candidate genes, RCI2c, we demonstrated its function in leaf venation patterning.
Casein related amyloid, characterization of a new and unique amyloid protein isolated from bovine corpora amylacea
Niewold, T.A. ; Murphy, C.L. ; Hulskamp-Koch, C.A.M. ; Tooten, P.C.J. ; Gruys, E. - \ 1999
Amyloid: international journal of experimental and clinical investigation 6 (1999). - ISSN 1350-6129 - p. 244 - 249.