Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Urinary excretion of advanced glycation end products in dogs and cats
    Palaseweenun, Pornsucha ; Hagen-Plantinga, Esther A. ; Schonewille, Thomas J. ; Koop, Gerrit ; Butre, Claire ; Jonathan, Melliana ; Wierenga, Peter A. ; Hendriks, Wouter H. - \ 2020
    Journal of Animal Physiology and Animal Nutrition (2020). - ISSN 0931-2439
    advanced glycation end products - cats - diet - dogs - urinary excretion

    The present study was conducted with privately owned dogs and cats to investigate whether a relationship exists between the dietary AGEs and the urinary excretion of AGEs, as indication of possible effective absorption of those compounds in the intestinal tract of pet carnivores. For this purpose, data were collected from both raw fed and dry processed food (DPF) fed to dogs and cats, through spot urine sampling and questionnaires. Raw pet food (RF, low in AGE diets) was fed as a primary food source to 29 dogs and DPF to 28 dogs. Cats were categorized into 3 groups, which were RF (n = 15), DPF (n = 14) and dry and wet processed pet food (DWF, n = 25). Urinary-free carboxymethyllysine (CML), carboxyethyllysine (CEL) and lysinoalanine (LAL) were analysed using ultrahigh-performance liquid chromatography (UHPLC)—mass spectrometry, and were standardized for variable urine concentration by expressing the AGE concentrations as a ratio to urine creatinine (Ucr) concentration (µg/µmol Ucr). Urinary excretion of CML, CEL and LAL in dogs fed with DPF was 2.03, 2.14 and 3 times higher compared to dogs fed with RF (p <.005). Similar to the dogs, a significant difference in CML:Ucr, CEL:Ucr and LAL:Ucr between the three diet groups was observed in cats (p-overall < 0.005, ANOVA), in which the RF fed group excreted less AGEs than the other groups. Linear regression coefficients and SE of CML:Ucr, CEL:Ucr and LAL:Ucr showed that body weight and neuter status were significantly correlated with CML and CEL excretion, but not to LAL excretion. Our results revealed a significant correlation between dietary AGEs and urinary excretion of free CML, CEL and LAL, and also showed that endogenous formation of these AGEs occurs in both dogs and cats under physiological conditions.

    GH3 Auxin-Amido Synthetases Alter the Ratio of Indole-3-Acetic Acid and Phenylacetic Acid in Arabidopsis
    Aoi, Yuki ; Tanaka, Keita ; Cook, Sam David ; Hayashi, Ken Ichiro ; Kasahara, Hiroyuki - \ 2020
    Plant and Cell Physiology 61 (2020)3. - ISSN 0032-0781 - p. 596 - 605.
    Arabidopsis - Auxin - Biosynthesis - Inactivation - Indole-3-acetic acid - Phenylacetic acid

    Auxin is the first discovered plant hormone and is essential for many aspects of plant growth and development. Indole-3-acetic acid (IAA) is the main auxin and plays pivotal roles in intercellular communication through polar auxin transport. Phenylacetic acid (PAA) is another natural auxin that does not show polar movement. Although a wide range of species have been shown to produce PAA, its biosynthesis, inactivation and physiological significance in plants are largely unknown. In this study, we demonstrate that overexpression of the CYP79A2 gene, which is involved in benzylglucosinolate synthesis, remarkably increased the levels of PAA and enhanced lateral root formation in Arabidopsis. This coincided with a significant reduction in the levels of IAA. The results from auxin metabolite quantification suggest that the PAA-dependent induction of GRETCHEN HAGEN 3 (GH3) genes, which encode auxin-amido synthetases, promote the inactivation of IAA. Similarly, an increase in IAA synthesis, via the indole-3-acetaldoxime pathway, significantly reduced the levels of PAA. The same adjustment of IAA and PAA levels was also observed by applying each auxin to wild-type plants. These results show that GH3 auxin-amido synthetases can alter the ratio of IAA and PAA in plant growth and development.

    Is livestock grazing a key factor for changing vegetation patterns in lime rich coastal dunes in the Netherlands?
    Hagen, Harrie G.J.M. van der; Assendorp, Dan ; Calame, Wim ; Meulen, Frank van der; Sýkora, Karlè V. ; Schaminée, Joop H.J. - \ 2020
    Journal of Coastal Conservation 24 (2020)2. - ISSN 1400-0350
    Aerial photographs - Blowout - Coastal dunes - Hippophae rhamnoides - Livestock grazing - Oryctolagus cuniculus

    In 1990, livestock grazing was introduced in Meijendel, a 1800 ha lime-rich coastal dune area, at a density of 0.06–0.07 LLU.ha-1.year−1 (1:12–18 ha) to counteract encroachment of tall grasses and shrubland on dune grassland and increase the bare sand area. Monitoring was based on four digital orthophotos (1975–1990–2001-2009) with a high spatial resolution (pixel size 25 × 25 cm). The changes were tested using Generalized Estimating Equations. Habitat changes occurred, but contradicting our hypothesis, there was no significant impact from the grazing on bare sand, grassland or shrubland within 11 and 19 years post livestock introduction. (1) After several decennia of decreasing bare sand, there was a significant increase between 2001 and 2009, irrespective of livestock presence. (2) The changes in grasslands and shrublands are independent of the livestock, but dependent on distance to the coast. (3) Bare sand and shrub cover determine the space left for the dune grasslands. It appears other factors than livestock grazing must have induced the changes. Changes in climate conditions and nitrogen load might have stimulated bare sand. An interaction with the end of Marram planting in 1990 cannot be concluded from available data. The disease-led reduction of rabbit grazing from the mid-1950s led to an expansion of the dominant shrub Hippophae rhamnoides. However, Hippophae shrubland typically regresses to grasslands on its collapse after 25–40 years. Tree species like Crataegus, Betula and Quercus will gradually dominate the landscape for far longer. Active removal of these indigenous species is necessary to prevent future loss of dune grasslands.

    The two-stage game approach to coalition formation: Where we stand and ways to go
    Hagen, Achim ; Mouche, Pierre von; Weikard, Hans Peter - \ 2020
    Games 11 (2020)1. - ISSN 2073-4336
    Binary action game - Cartel game - Coalition formation - Cournot-like game - Equilibrium coalition structure - Potential game - Symmetric game - Two-stage game

    Coalition formation is often analysed in an almost non-cooperative way, as a two-stage game that consists of a first stage comprising membership actions and a second stage with physical actions, such as the provision of a public good. We formalised this widely used approach for the case where actions are simultaneous in each stage. Herein, we give special attention to the case of a symmetric physical game. Various theoretical results, in particular, for cartel games, are provided. As they are crucial, recent results on the uniqueness of coalitional equilibria of Cournot-like physical games are reconsidered. Various concrete examples are included. Finally, we discuss research strategies to obtain results about equilibrium coalition structures with abstract physical games in terms of qualitative properties of their primitives.

    Geen invloed waterwinning op herstelde duinvalleien
    Heusden, Tom van; Hagen, H.G.J.M. van der; Schaminee, J.H.J. - \ 2019
    Holland's Duinen (2019)74. - ISSN 1389-7373 - p. 28 - 35.
    Een van de meest biodiverse habitats in Nederland is de vochtige kalkrijke duinvallei (Natura 2000 habitattype 2190B). Vele ecologische gradiënten en een hoge natuurlijke dynamiek zorgen voor een uitgebalanceerd pallet aan niches en ecologische randvoorwaarden. Doordat de mens op allerlei manieren een stempel in het duingebied heeft gezet, is nog slechts drie tot vijf procent van het originele areaal aan Nederlandse vochtige, kalkrijke duinvalleien aanwezig (Council of the European Communities 1992). Sinds de jaren 90 zijn er echter grootschalige herstelmaatregelen getroffen in veel Nederlandse kustgebieden om de duinvalleien te herstellen. Dit artikel beschrijft het resultaat van 21 jaar herstelmaatregelen in drie valleien in Meijendel met in het bijzonder het succes van de Kikkervalleien.
    Glycaemic effects of isomaltulose in dogs
    Corbee, Ronald Jan ; Hagen-Plantinga, Esther A. ; Montserrat Malagarriga, M. ; Neumer, F. ; Theis, S. ; Bosch, G. - \ 2019
    In: Congress proceedings 23rd Congress of the European Society of Veterinary and Comparative Nutrition. - Grugliasco (Italy) : Università Degli studi di Torino - p. 196 - 196.
    Using faecal glucocorticoid metabolites as a method for assessing physiological stress in reindeer
    Meisfjord Jørgensen, Grete Helen ; Eilertsen, Svein Morten ; Hansen, Inger ; Hagen, Snorre B. ; Fløystad, Ida ; Palme, Rupert ; Ozkan-Gulzari, Seyda - \ 2019
    In: Proceedings of the 53rd Congress of the ISAE. - Wageningen : Wageningen Academic Publishers - ISBN 9789086863389 - p. 332 - 332.
    Distant Non-Obvious Mutations Influence the Activity of a Hyperthermophilic Pyrococcusfuriosus Phosphoglucose Isomerase
    Subramanian, Kalyanasundaram ; Mitusińska, Karolina ; Raedts, John ; Almourfi, Feras ; Joosten, Henk Jan ; Hendriks, Sjon ; Sedelnikova, Svetlana E. ; Kengen, Servé W.M. ; Hagen, Wilfred R. ; Góra, Artur ; Martins Dos Santos, Vitor A.P. ; Baker, Patrick J. ; Oost, John van der; Schaap, Peter J. - \ 2019
    Biomolecules 9 (2019)6. - ISSN 2218-273X
    Comulator - cupin phosphoglucose isomerase - Protein engineering - Pyrococcus furiosus - solvent access

    The cupin-type phosphoglucose isomerase (PfPGI) from the hyperthermophilic archaeon Pyrococcus furiosus catalyzes the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate. We investigated PfPGI using protein-engineering bioinformatics tools to select functionally-important residues based on correlated mutation analyses. A pair of amino acids in the periphery of PfPGI was found to be the dominant co-evolving mutation. The position of these selected residues was found to be non-obvious to conventional protein engineering methods. We designed a small smart library of variants by substituting the co-evolved pair and screened their biochemical activity, which revealed their functional relevance. Four mutants were further selected from the library for purification, measurement of their specific activity, crystal structure determination, and metal cofactor coordination analysis. Though the mutant structures and metal cofactor coordination were strikingly similar, variations in their activity correlated with their fine-tuned dynamics and solvent access regulation. Alternative, small smart libraries for enzyme optimization are suggested by our approach, which is able to identify non-obvious yet beneficial mutations.

    Absorption, metabolism, distribution and excretion of (−)-epicatechin : A review of recent findings
    Borges, Gina ; Ottaviani, Javier I. ; Hooft, Justin J.J. van der; Schroeter, Hagen ; Crozier, Alan - \ 2018
    Molecular Aspects of Medicine 61 (2018). - ISSN 0098-2997 - p. 18 - 30.
    (–)-Epicatechin - Absorption - Disposition - Excretion - Flavan-3-ols - Metabolism

    This paper reviews pioneering human studies, their limitations and recent investigations on the absorption, metabolism, distribution and excretion (aka bioavailability) of (–)-epicatechin. Progress has been made possible by improvements in mass spectrometric detection when coupled to high performance liquid chromatography and through the increasing availability of authentic reference compounds of in vivo metabolites of (–)-epicatechin. Studies have shown that [2- 14 C](–)-epicatechin is absorbed in the small intestine with the 12 structural-related (–)-epicatechin metabolites (SREMs), mainly in the form of (–)-epicatechin-3′-O-glucuronide, 3′-O-methyl-(–)-epicatechin-5-sulfate and (–)-epicatechin-3′-sulfate, attaining sub-μmol/L peak plasma concentrations (C max ) ∼1 h after ingestion. SREMs were excreted in urine over a 24 h period in amounts corresponding to 20% of (–)-epicatechin intake. On reaching the colon the flavan-3-ol undergoes microbiota-mediated conversions yielding the 5C-ring fission metabolites (5C-RFMs) 5-(hydroxyphenyl)-γ-valerolactones and 5-(hydroxyphenyl)–γ-hydroxyvaleric acids which appear in plasma as phase II metabolites with a C max of 5.8 h after intake and are excreted in quantities equivalent to 42% of the ingested (–)-epicatechin. Other catabolites excreted in 0–24 h urine in amounts equivalent to 28% of intake included 3-(3′-hydroxyphenyl)hydracrylic acid, hippuric acid and 3′-hydroxyhippuric acid. Overall (–)-epicatechin is highly bioavailable with urinary excretion indicating that 95% is absorbed and passes through the circulatory systems as a diversity of phase II metabolites. Rats produce a very different profile of SREMs than that of humans. These findings demonstrate that ex vivo studies investigating the mechanisms underlying the protective effects of (–)-epicatechin on human health should make use of physiological concentrations human of SREMs and 5C-RFMs, and not the parent (–)-epicatechin, with model systems derived from human cells. In epidemiological studies 5-(4′-hydroxyphenyl)-γ-valerolactone-3′-sulfate and 5-(4′-hydroxyphenyl)-γ-valerolactone-3′-O-glucuronide, the principal 5C-RFMs in both plasma and urine, could serve as key biomarkers of (–)-epicatechin intake.

    Naar een bijvriendelijk beheer van hagen langs boerenland in Zuid-Limburg : Tussenrapport praktijknetwerk “Bijvriendelijk beheer van Zuid-Limburgse heggen”, onderdeel van Kennisimpuls Bestuivers
    Groot, G.A. de; Kats, R.J.M. van; Kleijn, D. ; Diaz Calafat, J. ; Kossen, H. - \ 2018
    - 4 p.
    Tussenrapport praktijknetwerk "Bijvriendelijk beheer van Zuid-Limburgse heggen", onderdeel van Kennisimpuls Bestuivers.
    Eighty Years of Mycopathologia: A Retrospective Analysis of Progress Made in Understanding Human and Animal Fungal Pathogens
    Chaturvedi, Vishnu ; Bouchara, Jean Philippe ; Hagen, Ferry ; Alastruey-Izquierdo, Ana ; Badali, Hamid ; Bocca, Anamelia Lorenzetti ; Cano-Lira, Jose F. ; Cao, Cunwei ; Chaturvedi, Sudha ; Chotirmall, Sanjay H. ; Diepeningen, Anne D. Van; Gangneux, Jean Pierre ; Guinea, Jesus ; Hoog, Sybren De; Ilkit, Macit ; Kano, Rui ; Liu, Weida ; Martinez-Rossi, Nilce M. ; Souza Carvalho Melhem, Marcia De; Ono, Mario Augusto ; Ran, Yuping ; Ranque, Stephane ; Almeida Soares, Celia Maria De; Sugita, Takashi ; Thomas, Philip A. ; Vecchiarelli, Anna ; Wengenack, Nancy L. ; Woo, Patrick C.Y. ; Xu, Jianping ; Zancope-Oliveira, Rosely M. - \ 2018
    Mycopathologia 183 (2018)6. - ISSN 0301-486X - p. 859 - 877.
    Mycopathologia was founded in 1938 to ‘diffuse the understanding of fungal diseases in man and animals among mycologists.’ This was an important mission considering that pathogenic fungi for humans and animals represent a tiny minority of the estimated 1.5–5 million fungal inhabitants on Earth. These pathogens have diverged from the usual saprotrophic lifestyles of most fungi to colonize and infect humans and animals. Medical and veterinary mycology is the subdiscipline of microbiology that dwells into the mysteries of parasitic, fungal lifestyles. Among the oldest continuing scientific publications on the subject, Mycopathologia had its share of ‘classic papers’ since the first issue was published in 1938. An analysis of the eight decades of notable contributions reveals many facets of host–pathogen interactions among 183 volumes comprising about 6885 articles. We have analyzed the impact and relevance of this body of work using a combination of citation tools (Google Scholar and Scopus) since no single citation metric gives an inclusive perspective. Among the highly cited Mycopathologia publications, those on experimental mycology accounted for the major part of the articles (36%), followed by diagnostic mycology (16%), ecology and epidemiology (15%), clinical mycology (14%), taxonomy and classification (10%), and veterinary mycology (9%). The first classic publication, collecting nearly 200 citations, appeared in 1957, while two articles published in 2010 received nearly 150 citations each, which is notable for a journal covering a highly specialized field of study. An empirical analysis of the publication trends suggests continuing interests in novel diagnostics, fungal pathogenesis, review of clinical diseases especially with relevance to the laboratory scientists, taxonomy and classification of fungal pathogens, fungal infections and carriage in pets and wildlife, and changing ecology and epidemiology of fungal diseases around the globe. We anticipate that emerging and re-emerging fungal pathogens will continue to cause significant health burden in the coming decades. It remains vital that scientists and physicians continue to collaborate by learning each other’s language for the study of fungal diseases, and Mycopathologia will strive to be their partner in this increasingly important endeavor to its 100th anniversary in 2038 and beyond.
    Carbon storage potential in degraded forests of Kalimantan, Indonesia
    Ferraz, António ; Saatchi, Sassan ; Xu, Liang ; Hagen, Stephen ; Chave, Jerome ; Yu, Yifan ; Meyer, Victoria ; Garcia, Mariano ; Silva, Carlos ; Roswintiart, Orbita ; Samboko, Ari ; Sist, Plinio ; Walker, Sarah ; Pearson, Timothy R.H. ; Wijaya, Arief ; Sullivan, Franklin B. ; Rutishauser, Ervan ; Hoekman, Dirk ; Ganguly, Sangram - \ 2018
    Environmental Research Letters 13 (2018)9. - ISSN 1748-9318
    aboveground biomass mapping - airborne lidar - carbon - forest degradation - Indonesia - Kalimantan - peat swamp forests

    The forests of Kalimantan are under severe pressure from extensive land use activities dominated by logging, palm oil plantations, and peatland fires. To implement the forest moratorium for mitigating greenhouse gas emissions, Indonesia's government requires information on the carbon stored in forests, including intact, degraded, secondary, and peat swamp forests. We developed a hybrid approach of producing a wall-to-wall map of the aboveground biomass (AGB) of intact and degraded forests of Kalimantan at 1 ha grid cells by combining field inventory plots, airborne lidar samples, and satellite radar and optical imagery. More than 110 000 ha of lidar data were acquired to systematically capture variations of forest structure and more than 104 field plots to develop lidar-biomass models. The lidar measurements were converted into biomass using models developed for 66 439 ha of drylands and 44 250 ha of wetland forests. By combining the AGB map with the national land cover map, we found that 22.3 Mha (106 ha) of forest remain on drylands ranging in biomass from 357.2 ±12.3 Mgha-1 in relatively intact forests to 134.2 ±6.1 Mgha-1 in severely degraded forests. The remaining peat swamp forests are heterogeneous in coverage and degradation level, extending over 3.62 Mha and having an average AGB of 211.8 ±12.7 Mgha-1. Emission factors calculated from aboveground biomass only suggest that the carbon storage potential of more than 15 Mha of degraded and secondary dryland forests will be about 1.1 PgC.

    Responses of summer phytoplankton biomass to changes in top-down forcing: Insights from comparative modelling
    Maar, Marie ; Butenschön, Momme ; Daewel, Ute ; Eggert, Anja ; Fan, Wei ; Hjøllo, Solfrid S. ; Hufnagl, Marc ; Huret, Martin ; Ji, Rubao ; Lacroix, Geneviève ; Peck, Myron A. ; Radtke, Hagen ; Sailley, Sévrine ; Sinerchia, Matteo ; Skogen, Morten D. ; Travers-Trolet, Morgane ; Troost, Tineke A. ; De Wolfshaar, Karen Van - \ 2018
    Ecological Modelling 376 (2018). - ISSN 0304-3800 - p. 54 - 67.
    Plankton functional types - Trophic cascades - Zooplankton mortality - Phytoplankton - ensemble modelling
    The present study describes the responses of summer phytoplankton biomass to changes in top-down forcing (expressed as zooplankton mortality) in three ecosystems (the North Sea, the Baltic Sea and the Nordic Seas) across different 3D ecosystem models. In each of the model set-ups, we applied the same changes in the magnitude of mortality (±20%) of the highest trophic zooplankton level (Z1). Model results showed overall dampened responses of phytoplankton relative to Z1 biomass. Phytoplankton responses varied depending on the food web structure and trophic coupling represented in the models. Hence, a priori model assumptions were found to influence cascades and pathways in model estimates and, thus, become highly relevant when examining ecosystem pressures such as fishing and climate change. Especially, the different roles and parameterizations of additional zooplankton groups grazed by Z1, and their importance for the outcome, emphasized the need for
    better calibration data. Spatial variability was high within each model indicating that physics (hydrodynamics and temperature) and nutrient dynamics also play vital roles for ecosystem responses to top-down effects. In conclusion, the model comparison indicated that changes in top-down forcing in combination with the modelled food-web structure affect summer phytoplankton biomass and, thereby, indirectly influence water quality of the systems.
    Natural feeding of the cat and dog and the idiosyncrasies of these carnivores
    Bosch, G. ; Hagen-Plantinga, Esther A. ; Hendriks, W.H. - \ 2017
    Evaluatie Actieplan Stalbranden 2012-2016
    Bokma-Bakker, Martien ; Bokma, Sjoerd ; Ellen, Hilko ; Hagen, René ; Ruijven, Charlotte van - \ 2017
    Wageningen : Wageningen Livestock Research (Wageningen Livestock Research rapport 1035) - 80
    dierenwelzijn - dierlijke productie - diergezondheid - pluimvee - varkens - melkvee - schapen - geiten - paarden - stallen - brand - voorkomen van branden - veiligheid - animal welfare - animal production - animal health - poultry - pigs - dairy cattle - sheep - goats - horses - stalls - fire - fire prevention - safety
    A novel environmental azole resistance mutation in Aspergillus fumigatus and a possible role of sexual reproduction in its emergence
    Zhang, Jianhua ; Snelders, Eveline ; Zwaan, Bas J. ; Schoustra, Sijmen E. ; Meis, Jacques F. ; Dijk, Karin van; Hagen, Ferry ; Beek, Martha T. van der; Kampinga, Greetje A. ; Zoll, Jan ; Melchers, Willem J.G. ; Verweij, Paul E. ; Debets, Fons - \ 2017
    mBio 8 (2017)3. - ISSN 2161-2129
    Ascospores - Aspergillus fumigatus - Azole resistance - Compost heap - Conidiospores - Hot spot for resistance development - Novel mutation - Sexual reproduction

    This study investigated the dynamics of Aspergillus fumigatus azoleresistant phenotypes in two compost heaps with contrasting azole exposures: azole free and azole exposed. After heat shock, to which sexual but not asexual spores are highly resistant, the azole-free compost yielded 98% (49/50) wild-type and 2% (1/50) azole-resistant isolates, whereas the azole-containing compost yielded 9% (4/45) wild-type and 91% (41/45) resistant isolates. From the latter compost, 80% (36/45) of the isolates contained the TR46/Y121F/T289A genotype, 2% (1/45) harbored the TR46/Y121F/M172I/T289A/G448S genotype, and 9% (4/45) had a novel pan-triazoleresistant mutation (TR46 3/Y121F/M172I/T289A/G448S) with a triple 46-bp promoter repeat. Subsequent screening of a representative set of clinical A. fumigatus isolates showed that the novel TR46 3 mutant was already present in samples from three Dutch medical centers collected since 2012. Furthermore, a second new resistance mutation was found in this set that harbored four TR46 repeats. Importantly, in the laboratory, we recovered the TR46 3 mutation from a sexual cross between two TR46 isolates from the same azole-containing compost, possibly through unequal crossing over between the double tandem repeats (TRs) during meiosis. This possible role of sexual reproduction in the emergence of the mutation was further implicated by the high level of genetic diversity of STR genotypes in the azole-containing compost. Our study confirms that azole resistance mutations continue to emerge in the environment and indicates compost containing azole residues as a possible hot spot. Better insight into the biology of environmental resistance selection is needed to retain the azole class for use in food production and treatment of Aspergillus diseases. IMPORTANCE Composting of organic matter containing azole residues might be important for resistance development and subsequent spread of resistance mutations in Aspergillus fumigatus. In this article, we show the dominance of azoleresistant A. fumigatus in azole-exposed compost and the discovery of a new resistance mutation with clinical relevance. Furthermore, our study indicates that current fungicide application is not sustainable as new resistance mutations continue to emerge, thereby threatening the use of triazoles in medicine. We provide evidence that the sexual part of the fungal life cycle may play a role in the emergence of resistance mutations because under laboratory conditions, we reconstructed the resistance mutation through sexual crossing of two azole-resistant A. fumigatus isolates derived from the same compost heap. Understanding the mechanisms of resistance selection in the environment is needed to design strategies against the accumulation of resistance mutations in order to retain the azole class for crop protection and treatment of Aspergillus diseases.

    Retorting conditions affect palatability and physical characteristics of canned cat food
    Hagen-Plantinga, E.A. ; Orlanes, D.F. ; Bosch, G. ; Hendriks, W.H. ; Poel, A.F.B. van der - \ 2017
    Journal of Nutritional Science 6 (2017). - ISSN 2048-6790 - 5 p.
    thermal processing - palatability - Texture - Viscosity - Canned food - Cats
    The effects of different temperature and time conditions during retorting of canned cat food on physicochemical characteristics and palatability were examined. For this purpose, lacquer cans containing an unprocessed loaf-type commercial cat food were heated in a pressurised retorting system at three specified temperature–time profiles (113°C/232 min, 120°C/103 min and 127°C/60 min) to equal a similar lethality (F 0 value = 30). Physicochemical properties (viscosity, texture, particle size, pH) were determined, and a 10 d three-bowl palatability test was performed with ten European shorthair cats. Retorting at 113°C/232 min resulted in differences in all the physical parameters examined ( particle size). Significant pH differences were observed (6·53, 6·63 and 6·66 for T113/232, 120 and 127°C, respectively). Preference ratios were 0·38, 0·31 and 0·31 for T113/232, 120 and 127°C, respectively (P = 0·067). It can be concluded that different retorting temperature–time profiles with equal F 0 value significantly affect physical characteristics and tended to affect palatability of moist cat food.
    Walnotenboomgaard als pensioenvoorziening : bomen verdienen een plek in de landbouw, ter inspiratie een permacultuurontwerp
    Zandbergen, Jelmer - \ 2017
    Ekoland (2017)3. - ISSN 0926-9142 - p. 14 - 15.
    walnoten - boomgaarden - juglon - agroforestry - bomen voor meerdere doeleinden - ecosysteemdiensten - walnuts - orchards - juglone - agroforestry - multipurpose trees - ecosystem services
    Na de tweede wereldoorlog werden akkers en weilanden groter. Veel bomen werden gerooid en 200.000 km aan hagen verdween. Met de bijbehorende ecosysteemdiensten, zoals koolstofbinding en een microklimaat waar gewassen en dieren van profiteren. Nu is er weer meer aandacht voor het terugbrengen van bomen en hagen in de landbouw, zeker als ze naast de ecosysteemdiensten ook voedsel en hout produceren
    Measurement of allergen-specific IgG in serum is of limited value for the management of dogs diagnosed with cutaneous adverse food reactions
    Hagen-Plantinga, E.A. ; Leistra, M.H.G. ; Sinke, J.D. ; Vroom, M.W. ; Savelkoul, H.F.J. ; Hendriks, W.H. - \ 2017
    The Veterinary Journal 220 (2017). - ISSN 1090-0233 - p. 111 - 116.
    Cutaneous adverse food reactions - Dogs - Food allergens - Food allergy - IgG ELISA
    Conflicting results have been reported in the literature in terms of the usefulness of serological testing for IgG against food allergens in dogs with cutaneous adverse food reaction (CAFR). The aim of the present study was to evaluate the suitability of a commercially available IgG ELISA for identifying food allergens in dogs, by challenging dogs with specific food ingredients, selected on the basis of IgG reactivity in serum samples. A total of 24 adult dogs with CAFR were enrolled into the study and 16 healthy dogs were included as a control group. Blood samples were obtained for measurement of specific IgG antibodies against 39 commonly used pet food ingredients by ELISA. Participating owners were surveyed to obtain information on their pet's dietary history. Eleven healthy control dogs and 12 dogs with CAFR were subsequently challenged in a blinded cross-over design experiment with both positive and negative food ingredients, selected on the basis of the ELISA test results. There was substantial individual variation in ELISA test results to the various food allergens, but no significant difference in IgG reactivity comparing the CAFR and control groups. None of the control dogs developed any clinical signs of an allergic reaction during the dietary challenge study. In the CAFR group, six of 12 dogs developed clinical signs after the negative challenge, and two of nine dogs developed clinical signs after the positive challenge. It was concluded that the ELISA test for dietary allergen-specific IgG is of limited value in the management of dogs with CAFR.
    Retorting conditions affect palatability and physical characteristics of canned cat food
    Hagen-Plantinga, E.A. ; Orlanes, D.F. ; Bosch, G. ; Hendriks, W.H. ; Poel, A.F.B. van der - \ 2016
    In: Growing science in pet nutrition. - Waltham Centre for Pet Nutrition - p. 175 - 176.
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