Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Molecular characterization of natural hybrids of Phytophthora nicotianae and P. cactorum
    Bonants, P.J.M. ; Hagenaar-de Weerdt, M. ; Man in 't Veld, W.A. ; Baayen, R.P. - \ 2000
    Phytopathology 90 (2000)8. - ISSN 0031-949X - p. 867 - 874.
    Hybrid isolates of Phytophthora nicotianae x P. cactorum from five different hosts (Cyclamen, Lavandula, Lewisia, Primula, and Spathiphyllum spp.) were identified by their atypical morphology and their well-defined heterozygous isozyme patterns. The hybrid nature of these isolates was tested by restriction fragment length polymorphism analysis of the internal transcribed spacer (ITS) region of rDNA, generating fragments typical for both P. nicotianae and P. cactorum. In hybrid isolates, polymerase chain reactions (PCR) with primers derived from unique parts of the ITS region (ITS-PCR) of both species yielded a combination of unique amplicons typical of both parental species. Eleven hybrid isolates, three isolates of each parental species and two atypical isolates from Rhododendron and Idesia spp. close to P. cactorum, were analyzed for amplified fragment length polymorphisms (AFLP). Consistent differences in AFLP patterns existed among the hybrid isolates, strongly indicating that these hybrids have arisen from independent hybridization events between P. nicotianae and P. cactorum. The two atypical isolates morphologically resembling P. cactorum were identical to the latter species in ITS-restriction fragment length polymorphism and response to the specific PCR primers but were intermediate between P. nicotianae x P. cactorum and P. cactorum in isozyme profiles and AFLP patterns. Since the introduction of hydroponic systems in greenhouses in the Netherlands, outbreaks of Phytophthora diseases are occurring in previously unaffected host species. This may be due to interspecific hybridization events resulting in novel pathogenic behavior.
    AFLP DNA fingerprinting of plantpathogenic fungi at IPO-DLO
    Bonants, P. ; Hagenaar-de Weerdt, M. ; Kema, G. ; Boogert, P. van den; Waalwijk, C. ; Baayen, R. - \ 1999
    Petria 9 (1999). - p. 97 - 104.
    Genetics of biological and molecular markers in Mycosphaerella graminicola, the cause of Septoria tritici leaf blotch of wheat
    Kema, G.H.J. ; Verstappen, E.C.P. ; Waalwijk, C. ; Bonants, P.J.M. ; Koning, J.R.A. de; Hagenaar-de Weerdt, M. ; Hamza, S. ; Koeken, J.G.P. ; Lee, T.A.J. van der - \ 1999
    In: Septoria on cereals / Lucas, J.A., Bowyer, P., Anderson, H.M., - p. 161 - 180.
    The first linkage map of Mycosphaerella graminicola (anamorph Septoria tritici), Europe's major fungal wheat pathogen.
    Kema, G.H.J. ; Hamza, S. ; Bonants, P.J.M. ; Waalwijk, C. ; Hagenaar-de Weerdt, M. ; Verstappen, E.C.P. ; Lee, T.A.J. van der - \ 1998
    In: 6th International Mycological Congress, Jerusalem, Israel - p. 81 - 81.
    Een koppelingskaart van het Mycosphaerella graminicola [anamorph Septoria tritici] genoom.
    Kema, G.H.J. ; Hamza, S. ; Bonants, P.J.M. ; Waalwijk, C. ; Hagenaar-de Weerdt, M. ; Verstappen, E.C.P. ; Lee, T.A.J. van der - \ 1998
    Gewasbescherming 29 (1998). - ISSN 0166-6495 - p. 55 - 56.
    The use of nested PCR for the detection of Phytophthora fragariae in strawberry, pp. 43-45
    Bonants, P.J.M. ; Gent-Pelzer, M.P.E. van; Hagenaar-de Weerdt, M. ; Cooke, D.E.L. ; Lacourt, I. ; Duncan, J.M. - \ 1997
    In: Developments in Plant Pathology, Proceedings of the 4th International Symposium of the European Foundation for Plant Pathology, September 9-12, 1996, Bonn, Germany, eds. H.W. Dehne et al
    Identification of race-specific markers of Phytophthora fragariae with AFLP, pp. 199-201
    Bonants, P.J.M. ; Hagenaar-de Weerdt, M. ; Cooke, D.E.L. ; Duncan, J.M. - \ 1997
    In: Developments in Plant Pathology, Proceedings of the 4th International Symposium of the European Foundation for Plant Pathology, September 9-12, 1996, Bonn, Germany, eds. H.W. Dehne et al
    Detection and identification of Phytophthora fragariae Hickman by the polymerase chain reaction
    Bonants, P. ; Hagenaar-de Weerdt, M. ; Gent-Pelzer, M. van; Lacourt, I. ; Cooke, D. ; Duncan, J. - \ 1997
    European Journal of Plant Pathology 103 (1997). - ISSN 0929-1873 - p. 345 - 355.
    Phytophthora fragariae Hickman, which causes strawberry red stele and raspberry root rot, is a quarantine organism for which specific and sensitive detection methods are required to test the health of planting material. Sequences of the internal transcribed spacer regions of the ribosomal gene repeat (rDNA) were used to develop primers for P. fragariae in a nested Polymerase Chain Reaction (PCR). The fungus was readily detected in infected but symptomless roots by nested, but not single-round, PCR. It was also detected in infested water samples obtained from the Dutch General Inspection Service by nested PCR. Detection of PCR products was at least 10-fold more sensitive by PCR-ELISA than by conventional visualisation on agarose gels.
    The use of nested PCR in the polymerase chain reaction for the detection of Phytophthora fragariae and P. cactorum in strawberry,
    Lacourt, I. ; Bonants, P.J.M. ; Gent-Pelzer, M.P. van; Cooke, D.E.L. ; Hagenaar-de Weerdt, M. ; Surplus, L. ; Duncan, J.M. - \ 1997
    In: Proceedings of the 3rd International Strawberry Symposium, 29 April - 4 May 1996, Veldhoven, the Netherlands / van der Scheer, H.A.T.H., Lieten, F., Dijkstra, J., - p. 829 - 838.
    Phytophthora fragariae var. fragariae Hickman, which causes red core disease of strawberry, is a quarantine organism on which a nil tolerance is placed. Detection of the fungus is by a root tip bait test which is highly specific and sensitive but time-consuming (5–6 weeks), has to be done at 12°C and can require some taxonomic experience. Even with the test, detecting red core can still be difficult, especially with early primary infection of propagation beds or infected symptomless resistant cultivars. Moreover, P. cactorum, a closely related fungus, causes crown rot of strawberry. It is not a quarantine organism, typical tolerances of 1␒nfection are allowed, and can confuse diagnosis. Techniques based to the Polymerase Chain Reaction (PCR) are promising alternatives. Various parts of the ribosomal RNA gene repeat (rDNA) have been used to design primers with different specificities: specific PCR primers that can differentiate between P. fragariae and P. cactorum and others designed to detect a wider range of Peronosporales, to which Phytophthora belongs. In nested PCR, combinations of these primers can detect both pathogens with high specificity and sensitivity. Competitive PCR has also been developed to allow quantification of P. cactorum infection in strawberry crowns. With these techniques, P. fragariae was detected in strawberry plants one day after inoculation whereas the symptoms were only visible after one week. Moreover, very low numbers of zoospores could be detected in water samples. The test has been evaluated on samples provided by the inspection services of Scotland and The Netherlands.
    The use of nested primers in PCR for the detection of Phytophthora fragariae in strawberry
    Bonants, P.J.M. ; Hagenaar-de Weerdt, M. - \ 1995
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