Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Onderzoek: hoe leer je kinderen groente eten
    Zeinstra, Gertrude - \ 2020
    Groenten kweken op waterplas
    Vijn, Marcel - \ 2017
    Down-regulation of Arabidopsis DND1 orthologs in potato and tomato leads to broad-spectrum resistance to late blight and powdery mildew
    Sun, K. ; Wolters, A.M.A. ; Loonen, A.E.H.M. ; Huibers, R.P. ; Vlugt, R.A.A. van der; Goverse, A. ; Jacobsen, E. ; Visser, R.G.F. ; Bai, Y. - \ 2016
    Transgenic Research 25 (2016). - ISSN 0962-8819 - p. 123 - 138.
    Multiple susceptibility genes (S), identified in Arabidopsis, have been shown to be functionally conserved in crop plants. Mutations in these S genes result in resistance to different pathogens, opening a new way to achieve plant disease resistance. The aim of this study was to investigate the role of Defense No Death 1 (DND1) in susceptibility of tomato and potato to late blight (Phytophthora infestans). In Arabidopsis, the dnd1 mutant has broad-spectrum resistance against several fungal, bacterial, and viral pathogens. However this mutation is also associated with a dwarfed phenotype. Using an RNAi approach, we silenced AtDND1 orthologs in potato and tomato. Our results showed that silencing of the DND1 ortholog in both crops resulted in resistance to the pathogenic oomycete P. infestans and to two powdery mildew species, Oidium neolycopersici and Golovinomyces orontii. The resistance to P. infestans in potato was effective to four different isolates although the level of resistance (complete or partial) was dependent on the aggressiveness of the isolate. In tomato, DND1-silenced plants showed a severe dwarf phenotype and autonecrosis, whereas DND1-silenced potato plants were not dwarfed and showed a less pronounced autonecrosis. Our results indicate that S gene function of DND1 is conserved in tomato and potato. We discuss the possibilities of using RNAi silencing or loss-of-function mutations of DND1 orthologs, as well as additional S gene orthologs from Arabidopsis, to breed for resistance to pathogens in crop plants.
    Natural loss-of-function mutation of EDR1 conferring resistance to tomato powdery mildew in Arabidopsis thaliana accession C24
    Gao, D. ; Appiano, M. ; Huibers, R.P. ; Loonen, A.E.H.M. ; Visser, R.G.F. ; Wolters, A.M.A. ; Bai, Y. - \ 2015
    Molecular Plant Pathology 16 (2015)1. - ISSN 1464-6722 - p. 71 - 82.
    salicylic-acid - downy mildew - gene - defense - plants - microsatellites - mechanism - evolution - cloning - kinase
    To screen for potentially novel types of resistance to tomato powdery mildew Oidium neolycopersici, a disease assay was performed on 123 Arabidopsis thaliana accessions. Forty accessions were fully resistant, and one, C24, was analysed in detail. By quantitative trait locus (QTL) analysis of an F2 population derived from C24 × Sha (susceptible accession), two QTLs associated with resistance were identified in C24. Fine mapping of QTL-1 on chromosome 1 delimited the region to an interval of 58¿kb encompassing 15 candidate genes. One of these was Enhanced Disease Resistance 1 (EDR1). Evaluation of the previously obtained edr1 mutant of Arabidopsis accession Col-0, which was identified because of its resistance to powdery mildew Golovinomyces cichoracearum, showed that it also displayed resistance to O.¿neolycopersici. Sequencing of EDR1 in our C24 germplasm (referred to as C24-W) revealed two missing nucleotides in the second exon of EDR1 resulting in a premature stop codon. Remarkably, C24 obtained from other laboratories does not contain the EDR1 mutation. To verify the identity of C24-W, a DNA region containing a single nucleotide polymorphism (SNP) unique to C24 was sequenced showing that C24-W contains the C24-specific nucleotide. C24-W showed enhanced resistance to O.¿neolycopersici compared with C24 not containing the edr1 mutation. Furthermore, C24-W displayed a dwarf phenotype, which was not associated with the mutation in EDR1 and was not caused by the differential accumulation of pathogenesis-related genes. In conclusion, we identified a natural edr1 mutant in the background of C24.
    Activation tagging of ATHB13 in Arabidopsis thaliana confers broad-spectrum disease resistance
    Gao, D. ; Huibers, R.P. ; Chen, X. ; Loonen, A.E.H.M. ; Visser, R.G.F. ; Wolters, A.M.A. ; Bai, Y. - \ 2014
    Plant Molecular Biology 86 (2014)6. - ISSN 0167-4412 - p. 641 - 653.
    vegetative storage protein - powdery mildew resistance - transcription factors hahb1 - nudix hydrolase - salicylic-acid - plant defense - cell-death - hd-zip - oidium-neolycopersici - expression patterns
    Powdery mildew species Oidium neolycopersici (On) can cause serious yield losses in tomato production worldwide. Besides on tomato, On is able to grow and reproduce on Arabidopsis. In this study we screened a collection of activation-tagged Arabidopsis mutants and identified one mutant, 3221, which displayed resistance to On, and in addition showed a reduced stature and serrated leaves. Additional disease tests demonstrated that the 3221 mutant exhibited resistance to downy mildew (Hyaloperonospora arabidopsidis) and green peach aphid (Myzus persicae), but retained susceptibility to bacterial pathogen Pseudomonas syringae pv tomato DC3000. The resistance trait and morphological alteration were mutually linked in 3221. Identification of the activation tag insertion site and microarray analysis revealed that ATHB13, a homeodomain-leucine zipper (HD-Zip) transcription factor, was constitutively overexpressed in 3221. Silencing of ATHB13 in 3221 resulted in the loss of both the morphological alteration and resistance, whereas overexpression of the cloned ATHB13 in Col-0 and Col-eds1-2 backgrounds resulted in morphological alteration and resistance. Microarray analysis further revealed that overexpression of ATHB13 influenced the expression of a large number of genes. Previously, it was reported that ATHB13-overexpressing lines conferred tolerance to abiotic stress. Together with our results, it appears that ATHB13 is involved in the crosstalk between abiotic and biotic stress resistance pathways.
    Down-regulation of acetolactate synthase compromises OI-1- mediated resistance to powdery mildew in tomato
    Gao, D. ; Huibers, R.P. ; Loonen, A.E.H.M. ; Visser, R.G.F. ; Wolters, A.M.A. ; Bai, Y. - \ 2014
    BMC Plant Biology 14 (2014). - ISSN 1471-2229 - 11 p.
    glutamate-dehydrogenase gene - acetohydroxyacid synthase - monogenic-resistance - defense responses - nicotiana-tabacum - ol-genes - arabidopsis - plants - inhibition - biosynthesis
    Background - In a cDNA-AFLP analysis comparing transcript levels between powdery mildew (Oidium neolycopersici)-susceptible tomato cultivar Moneymaker (MM) and near isogenic lines (NILs) carrying resistance gene Ol-1 or Ol-4, a transcript-derived fragment (TDF) M11E69-195 was found to be present in NIL-Ol-1 but absent in MM and NIL-Ol-4. This TDF shows homology to acetolactate synthase (ALS). ALS is a key enzyme in the biosynthesis of branched-chain amino acids valine, leucine and isoleucine, and it is also a target of commercial herbicides. Results - Three ALS homologs ALS1, ALS2, ALS3 were identified in the tomato genome sequence. ALS1 and ALS2 show high similarity, whereas ALS3 is more divergent. Transient silencing of both ALS1 and ALS2 in NIL-Ol-1 by virus-induced gene silencing (VIGS) resulted in chlorotic leaf areas that showed increased susceptibility to O. neolycopersici (On). VIGS results were confirmed by stable transformation of NIL-Ol-1 using an RNAi construct targeting both ALS1 and ALS2. In contrast, silencing of the three ALS genes individually by RNAi constructs did not compromise the resistance of NIL-Ol-1. Application of the herbicide chlorsulfuron to NIL-Ol-1 mimicked the VIGS phenotype and caused loss of its resistance to On. Susceptible MM and On-resistant line NIL-Ol-4 carrying a nucleotide binding site and leucine rich repeat (NB-LRR) resistance gene were also treated with chlorsulfuron. Neither the susceptibility of MM nor the resistance of NIL-Ol-4 was affected. Conclusions - ALS is neither involved in basal defense, nor in resistance conferred by NB-LRR type resistance genes. Instead, it is specifically involved in Ol-1-mediated resistance to tomato powdery mildew, suggesting that ALS-induced change in amino acid homeostasis is important for resistance conferred by Ol-1.
    Powdery Mildew Resistance in Tomato by Impairment of SIPMR4 and SIDMR1
    Huibers, R.P. ; Loonen, A.E.H.M. ; Dongli Gao, Dongli ; Ackerveken, G. van den; Visser, R.G.F. ; Bai, Y. - \ 2013
    PLoS ONE 8 (2013)6. - ISSN 1932-6203
    zinc-finger nucleases - disease resistance - arabidopsis mutants - targeted mutagenesis - gene - plants - pathogenesis - mutation
    Genetic dissection of disease susceptibility in Arabidopsis to powdery and downy mildew has identified multiple susceptibility (S) genes whose impairment results in disease resistance. Although several of these S-genes have been cloned and characterized in more detail it is unknown to which degree their function in disease susceptibility is conserved among different plant species. Moreover, it is unclear whether impairment of such genes has potential in disease resistance breeding due to possible fitness costs associated with impaired alleles. Here we show that the Arabidopsis PMR4 and DMR1, genes encoding a callose synthase and homoserine kinase respectively, have functional orthologs in tomato with respect to their S-gene function. Silencing of both genes using RNAi resulted in resistance to the tomato powdery mildew fungus Oidium neolycopersici. Resistance to O. neolycopersici by SlDMR1 silencing was associated with severely reduced plant growth whereas SlPMR4 silencing was not. SlPMR4 is therefore a suitable candidate gene as target for mutagenesis to obtain alleles that can be deployed in disease resistance breeding of tomato.
    A participatory modelling approach to define farm-scale effects of reclaimed wastewater irrigation in the Lockyer Valley, Australia
    Opstal, J.D. van; Huibers, F.P. ; Cresswell, R.G. - \ 2012
    Water International 37 (2012)7. - ISSN 0250-8060 - p. 843 - 858.
    simulation - systems - apsim - management - scarcity
    The Lockyer Valley is an important agricultural area experiencing water insecurity, which causes a decrease in agricultural production. Regional authorities are initiating a wastewater reclamation project conveying treated municipal wastewater to water users, including potentially the Lockyer Valley. This additional (and essentially reliable) water source will change farming options and practices. A participatory modelling approach was used to analyse the consequences of changed water availability at the farm scale. This approach incorporates both farmer and scientist knowledge and gives due attention to non-technical issues like perception and acceptance. Two cropping patterns were worked out to forecast the effects on water deficit and crop yield in the projected situation.
    Discuter les apporches conventionneles de gestion de l'utilisation des eaux usées en agriculture
    Huibers, F.P. ; Redwood, M. ; Raschid-Sally, L. - \ 2011
    In: L'irrigation avec des eaux usées et la santé: évaluer et atténuer les risques dans les paus à faible revenu / Scott, C.A., Raschid-Sally, L., Redwood, M., Bahri, A., Drechsel, P., Québec : Presses de l'Université du Québec - ISBN 9782760531604 - p. 309 - 325.
    Gebruikswaarde onderzoek laanbomen : Acer campestre 'Huibers Elegant'
    Hiemstra, J.A. ; Sluis, B.J. van der; Slingerland, L. ; Schalk, G. - \ 2011
    Wageningen : PPO
    Recognizing Wastewater as a Resource in Irrigated Agriculture
    Huibers, F.P. - \ 2011
    ICID news 2011 (2011)1. - p. 4 - 4.
    L'Utilisation des Eaux Usées. Session 3+5: La Chaine de l'eau. Le bassin versant urbain
    Huibers, Frans - \ 2010
    L'Utilisation des Eaux Usées. Session 2+4: Réalité, Tecnhnologies et Gestion
    Huibers, Frans - \ 2010
    L'Utilisation des Eaux Usées. Session 1: Introduction
    Huibers, Frans - \ 2010
    Wastewater Use in Irrigated Agriculture
    Huibers, Frans - \ 2010
    Wastewater: Water Scarcity Solution in Agriculture
    Huibers, Frans - \ 2010
    Governing Peri-Urban Waste Water Used by Farmers: Implications for Design and Management
    Grendelman, E.R. ; Huibers, F.P. - \ 2010
    In: Social Perspectives on the Sanitation Challenge. Pt. 3 / van Vliet, B.J.M., Spaargaren, G., Oosterveer, P.J.M., Dordrecht : Springer - ISBN 9789048137206 - p. 189 - 202.
    multi-stakeholder platforms - reuse - areas
    Worldwide, population is increasingly centralized in metropolitan areas. This has an impact on water systems and complex metropolitan watersheds emerge. Flows of varying water quality are generated and distributed among different users who develop new opportunities and coping mechanisms for dealing with marginal quality waters. In developing countries waste water management often fails to cope with the increasing number and volumes of flows. Financial and institutional limitations force waste water managers to discharge substantial amounts of untreated or partially treated waste water into surface waters. Consequently, use of polluted water is increasingly common in the downstream peri-urban agricultural areas. This, albeit productive, may lead to negative impacts on human health and environment, if management of this water is not rightly done. Mitigation of the problems requires rethinking of conventional ‘top-down’ waste water system design and management, in combination with expected down-stream use. In this chapter the applicability of water governance principles in design and operation of waste water systems with an effluent use component is investigated. Acknowledgement of the treatment potential of subsequent uses and the significance of use-based practices as opposed to zero-pollution design will certainly change design and treatment procedures. Inclusion of agriculture and nature as a treatment step and participation of users in decision-making are expected to optimize use of finances, infrastructure and personnel
    Challenging Conventional Approaches to Managing Wastewater Use in Agriculture
    Huibers, F.P. ; Redwood, M. ; Raschid-Sally, L. - \ 2010
    In: Wastewater Irrigation and Health. Assessing and Mitigating Risk in Low-Income Countries / Dreschsel, P., Scott, C.A., Raschid-Sally, L., Redwood, M., Bahri, A., London : Earthscan - ISBN 9781844077953 - p. 287 - 301.
    Thermo Elektrische Generator niet rendabel (n.a.v. de Haalbaarheidsstudie door M.H. Huibers en R.jansen)
    Jansen, R.M.C. - \ 2010
    Groenten en Fruit. Algemeen 2010 (2010)321. - ISSN 0925-9694
    From unplanned to planned agricultural use: making an asset out of wastewater
    Lier, J.B. van; Huibers, F.P. - \ 2010
    Irrigation and Drainage Systems 24 (2010)1-2. - ISSN 0168-6291 - p. 143 - 152.
    Urban wastewater is increasingly used for agricultural production, particularly in those areas where access to fresh water resources is limiting. Depending on the prevailing institutional arrangements, this agricultural use is planned or unplanned. If planned, a general policy is to minimise health risks and environmental pollution, leading to an often centralised collection of the city’s sewage, followed by primary, secondary and further treatment until the prevalent discharge effluent use standards are met. If society can afford them, advanced treatment technologies are installed, backed by a well functioning institutional infrastructure. In contrast, in less prosperous countries the agricultural use of wastewater is driven by the high needs for water and the absence of affordable fresh water resources along with insufficient financial means to construct treatment systems and distribution networks that comply with the official regulations. Combined with a poor institutional framework, this results in unplanned and unguided direct or indirect use of raw, partially treated or diluted wastewater. In an effort to mitigate health and environmental problems, we propose using a reverse water chain design approach, in which the ultimate fate of the water is the basis for the design of conveyance and treatment facilities.
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