Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    A short-term intervention with selenium affects expression of genes implicated in the epithelial-to-mesenchymal transition in the prostate
    Gils-Kok, Dieuwertje van; Kiemeney, Lambertus A.L.M. ; Verhaegh, Gerald W. ; Schalken, Jack A. ; Lin, Emile N.J.T. van; Sedelaar, J.P.M. ; Witjes, J.A. ; Hulsbergen-van de Kaa, Christina A. ; Veer, Pieter van 't; Kampman, Ellen ; Afman, Lydia - \ 2017
    Wageningen University
    GSE77959 - Homo sapiens - GSE77959 - Homo sapiens - PRJNA312203
    In parallel with the inconsistency in observational studies and chemoprevention trials, the molecular mechanisms by which selenium may affect prostate cancer risk have not been elucidated. We conducted a randomized, placebo-controlled intervention trial to examine the effects of a short-term intervention with selenized yeast on whole-genome expression profiles in non-malignant prostate tissue. Twenty-three men receiving prostate biopsies were randomly assigned to take 300 µg selenized yeast per day (n=12) or placebo (non-selenized yeast, n=11) during a median intervention period of 35 (interquartile range: 31-35) days. Prostate specimens, collected from the transition zone before and after intervention, of 15 participants (n=8 selenium, n=7 placebo) were available for analysis using Affymetrix GeneChip Human 1.0 ST Arrays. Pathway and gene set enrichment analyses revealed that the intervention with selenium resulted in a down-regulated expression of genes involved in signaling pathways related to cellular adhesion, migration, invasion, remodeling and immune responses. Specifically, expression of the well-established epithelial marker E-cadherin was up-regulated, while mesenchymal markers, such as vimentin and fibronectin, were down-regulated after the intervention with selenium. This implies an effect of selenium on the epithelial-to-mesenchymal transition (EMT). Moreover, selenium affected expression of genes involved in wound healing and inflammation, processes which are both related to EMT. In conclusion, our data showed that selenium affected expression of genes implicated in EMT, mainly represented by a change in the direction of the epithelial rather than the mesenchymal phenotype.
    A short-term intervention with selenium affects expression of genes implicated in the epithelial-to-mesenchymal transition in the prostate
    Gils-Kok, Dieuwertje van; Kiemeney, Lambertus A.L.M. ; Verhaegh, Gerald W. ; Schalken, Jack A. ; Lin, Emile N.J.T. van; Sedelaar, J.P.M. ; Witjes, J.A. ; Hulsbergen-van de Kaa, Christina A. ; Veer, Pieter van 't; Kampman, Ellen ; Afman, Lydia A. - \ 2017
    Oncotarget 8 (2017)6. - ISSN 1949-2553 - p. 10565 - 10579.
    EMT - Gene expression - Microarray - Prostatic neoplasms - Selenium

    In parallel with the inconsistency in observational studies and chemoprevention trials, the mechanisms by which selenium affects prostate cancer risk have not been elucidated. We conducted a randomized, placebo-controlled trial to examine the effects of a short-term intervention with selenium on gene expression in non-malignant prostate tissue. Twenty-three men received 300 μg selenium per day in the form of selenized yeast (n=12) or a placebo (n=11) during 5 weeks. Prostate biopsies collected from the transition zone before and after intervention were analysed for 15 participants (n=8 selenium, n=7 placebo). Pathway analyses revealed that the intervention with selenium was associated with down-regulated expression of genes involved in cellular migration, invasion, remodeling and immune responses. Specifically, expression of well-established epithelial markers, such as E-cadherin and epithelial cell adhesion molecule EPCAM, was up-regulated, while the mesenchymal markers vimentin and fibronectin were down-regulated after intervention with selenium. This implies an inhibitory effect of selenium on the epithelial-to-mesenchymal transition (EMT). Moreover, selenium was associated with down-regulated expression of genes involved in wound healing and inflammation; processes which are both related to EMT. In conclusion, our explorative data showed that selenium affected expression of genes implicated in EMT in the transition zone of the prostate.

    Selenium induces an anti-inflammatory gene expression profile in prostate tissue
    Kok, D.E.G. ; Kiemeney, L.A.L.M. ; Lin, E.N.J.T. van; Sedelaar, J.P.M. ; Witjes, J.A. ; Hulsbergen-van de Kaa, C.A. ; Veer, P. van 't; Müller, M.R. ; Kampman, E. ; Afman, L.A. - \ 2011
    Body mass index as a prognostic marker for biochemical recurrence in Dutch men treated with radical prostatectomy
    Roermund, J.G.H. van; Kok, D.E.G. ; Wildhagen, M.F. ; Kiemeney, L.A. ; Struik, F. ; Sloot, S. ; Oort, I.M. van; Hulsbergen- van de Kaa, C.A. ; Leenders, G.J.L.H. van; Bangma, C.H. ; Witjes, J.A. - \ 2009
    BJU International 104 (2009)3. - ISSN 1464-4096 - p. 321 - 325.
    cancer incidence - united-states - weight change - follow-up - obesity - risk - mortality - impact - prevalence - overweight
    OBJECTIVE To investigate whether body mass index (BMI) is a prognostic factor for biochemical recurrence (BCR) in Dutch men after radical prostatectomy (RP), as although epidemiological studies of obesity in relation to prostate cancer have provided conflicting results, recent studies from the USA suggest that a higher BMI is a risk factor for progression of prostate cancer. PATIENTS AND METHODS Of the 1417 patients with prostate cancer who had RP at two University hospitals, 1302 were included in the present study. BMI (kg/m(2)) classes were defined as normal (<25), overweight (25-30) and obese (>= 30). The median follow-up was 59 months and clinical data were obtained retrospectively from charts. BCR was defined as two consecutive prostate-specific antigen (PSA) levels of > 0.1 ng/mL. RESULTS In all, 600 patients were classified as having normal weight (43.9%), 665 as overweight (48.6%) and 103 as obese (7.5%). Overall, 297 patients developed BCR after RP; the 10-year risk (95% confidence interval) of BCR was 31.9 (26.6-37.2)%, 30.5 (25.8-35.2)% and 23.9 (14.9-32.9)% for patients in the three categories, respectively (P = 0.836). Multivariable proportional hazard regression analyses of BMI and established prognostic factors for BCR did not change these results. CONCLUSION BMI appeared to have no prognostic value for BCR in Dutch patients with clinically localized prostate cancer and treated with RP.
    A single institution experience with biochemical recurrence after radical prostatectomy for tumors that on pathology are of small volume or "insignificant"
    Oort, I.M. van; Kok, D.E.G. ; Kiemeney, L.A. ; Hulsbergen-van de Kaa, C.A. ; Witjes, J.A. - \ 2009
    Urology Oncology: Seminars and Original Investigations 27 (2009)5. - ISSN 1078-1439 - p. 509 - 513.
    retropubic prostatectomy - independent predictor - clinical-significance - needle-biopsy - cancer - specimens - men - progression - nomogram - features
    Objective: Small volume prostate cancers (0.10 ng/ml. Results: In the total group, the median age at the time Of Surgery was 62.7 years (42.4-73.4) and the median preoperative PSA level was 8.0 ng/ml. Patients with InsigPC had Gleason scores of 4 in 7%, 5 in 37%, and 6 in 56%. Positive surgical margins were identified in 13 (15.9%) svPC and in 8 (12.7%) InsigPC specimens. The 5-year risk of BCR for the svPC group and the insigPC group was 10% (95% Cl 2-18%, 7 and 5 patients, respectively) vs. 35% (95% CI 29-41%) in the rest of the cohort (log rank P = 0.001). Conclusion: Patients with svPC and patients with InsigPC have a significantly lower risk of BCR. However, even in this seemingly very favorable patient group, I in 10 patients will develop a BCR after RP. Therefore, new studies are needed to examine what the prognostic relevance is of small-volume tumors. (C) 2009 Elsevier Inc. All rights reserved.
    Maximum tumor diameter is not an independent prognostic factor in high-risk localized prostate cancer
    Oort, I.M. van; Witjes, J.A. ; Kok, D.E.G. ; Kiemeney, L.A. ; Hulsbergen-van de Kaa, C.A. - \ 2008
    World Journal of Urology 26 (2008)3. - ISSN 0724-4983 - p. 237 - 241.
    radical prostatectomy - multivariate-analysis - antigen recurrence - specimens - predictor - volume - carcinoma - failure - progression - men
    Previous studies suggest that maximum tumor diameter (MTD) is a predictor of recurrence in prostate cancer (PC). This study investigates the prognostic value of MTD for biochemical recurrence (BCR) in patients with PC, after radical prostatectomy (RP), with emphasis on high-risk localized prostate cancer. Methods RP specimens of 542 patients were evaluated with a median follow-up of 39.5 months (range 0.6¿150 months). MTD was defined as the largest diameter of the largest tumor; high-risk as ¿T2c or PSA level > 20 ng/ml or Gleason score ¿8 and BCR as two consecutive PSA levels > 0.10 ng/ml. Proportional hazards multivariable regression models were composed to determine prognostic factors for BCR. Results Overall, 114 patients developed BCR after RP. The overall 5-year risk of BCR was 25% (95% CI = 20.4¿29.6), and median MTD was 24 mm (range 1¿65). MTD in the total and high-risk group was associated with total tumor volume, volume of the largest tumor, pre-operative PSA levels, and Gleason score. In a univariable analyses, MTD was weakly associated with risk of BCR (HR = 1.02 per mm increase, 95% CI = 1.002¿1.035, P = 0.024) in the total group; in the high-risk group this association was lost (HR = 1.01, 95%CI = 0.99¿1.03, P = 0.18). Multivariable analyses indicated that positive surgical margins, higher Gleason score, advanced pathological stage, and multiple tumors were the main prognostic factors for BCR irrespective of the risk profile. MTD did not provide additional information. Conclusions MTD is not an independent prognostic factor for BCR in patients treated with RP, irrespective of the risk profile
    The prognostic role of the pathological T2 subclassification for prostate cancer in the 2002 Tumouor-Nodes-Metastasis staging system
    Oort, I.M. van; Witjes, J.A. ; Kok, D.E.G. ; Kiemeney, L.A. ; Hulsbergen-van de Kaa, C.A. - \ 2008
    BJU International 102 (2008)4. - ISSN 1464-4096 - p. 438 - 441.
    progression-free survival - american joint committee - radical prostatectomy - ability - disease - pt2b
    OBJECTIVE To evaluate the prognostic role of the 2002 Tumour-Nodes-Metastasis (TNM) pT2 subclassification for biochemical recurrence (BCR) after radical prostatectomy (RP) for prostate cancer. PATIENTS AND METHODS The 1997 TNM staging system is based on one subdivision for organ-confined prostate tumours (T2a, unilateral; T2b, bilateral involvement). The 2002 TNM staging system subdivides unilateral involvement into T2a (half of one lobe or less) and T2b (more than one half of one lobe), while bilateral involvement is classified as T2c. In all, 542 patients were treated with RP at our institute; the RP specimens were completely embedded and histopathologically evaluated for Gleason grade, tumour volume and anatomical extent, and were staged according to the 2002 TNM staging criteria. Patients were followed for a median of 39.5 months. BCR was defined as two subsequent increasing prostate-specific antigen (PSA) levels of >0.10 ng/mL. Kaplan-Meier and proportional hazards regression analyses were used to evaluate the univariable and multivariable prognostic effect of tumour stage. RESULTS According to the 2002 TNM staging system, 360 specimens were found to have pT2 tumours; 79 (22%) of the RP specimens were staged as pT2a and 281 (78%) as pT2c; no pT2b specimens were identified. Patients with unilateral involvement (pT2a) had a 5-year risk of BCR of 13%, while those with bilateral involvement (pT2c) had a risk of 23% (log rank test, P = 0.056). Patients with l disease were more likely to have a larger tumour volume (Mann-Whitney U-test P <0.001) and positive surgical margins (Fishers' exact test, P = 0.001)than those with pT2a tumours. Mann-Whitney U-tests showed no differences between the groups for preoperative PSA levels (P = 0.167). Also, the RP Gleason score was no different between groups (Pearson chi-square, P = 0.807). In the multivariable analyses, positive surgical margins appeared to increase the risk of BCR (hazard ratio 4.4, 95% confidence interval 2.5-7.9); pT2c vs pT2a had only a marginally (insignificant) additional effect (1.3, 0.6-2.7). CONCLUSION The absence of a true unilateral pathological T2b tumour in a series of 360 pT2 RP specimens questions the relevance of substaging unilateral disease. The limited differences in BCR and in pathological features of unilateral vs bilateral pT2 prostate cancer justify modifying the TNM staging system to one with no subclassification of pT2 disease, or at most as only one subdivision into unilateral (T2a) and bilateral (T2b) disease, combining the T2b and T2c substages.
    Vermindering van ammoniakemissie door mestkoeling bij gespeende biggen
    Beurskens-Voermans, M.P. ; Kaa, C.C.R. van der - \ 1997
    Rosmalen : Praktijkonderzoek varkenshouderij (Proefverslag / Praktijkonderzoek Varkenshouderij P4.23) - 8
    luchtverontreiniging - ammoniak - emissie - vervluchtiging - varkens - kraamstallen - stallen - koelen - koudeopwekking - air pollution - ammonia - emission - volatilization - pigs - farrowing houses - stalls - cooling - refrigeration
    In de periode april tot en met juni 1996 is op het Varkensproefbedrijf te Sterksel onderzoek verricht naar de ammoniakemissie bij gespeende biggen, gehuisvest op een volledig roostervloer. De toplaag van de mest werd gekoeld met behulp van het R&R koeldeksysteem.
    Ontwerp van biologische stikstofverwijderingssystemen voor varkensmest
    Kaa, C.C.R. van der; Gastel, J.P.B.F. van - \ 1997
    Rosmalen : Praktijkonderzoek varkenshouderij (Proefverslag / Praktijkonderzoek Varkenshouderij P1.192) - 68
    dierlijke meststoffen - drijfmest - varkens - rioolwater - afvalwater - stikstof - nitrificatie - denitrificatie - verwijdering - animal manures - slurries - pigs - sewage - waste water - nitrogen - nitrification - denitrification - removal
    Handleiding biologische stikstofverwijdering uit mest
    Gastel, J. van; Kaa, C. van der - \ 1997
    Praktijkonderzoek varkenshouderij 11 (1997)5. - ISSN 1382-0346 - p. 12 - 13.
    stalmest - varkens - dierlijke meststoffen - drijfmest - nitrificatie - denitrificatie - stikstof - prijzen - prijsvorming - kosten - farmyard manure - pigs - animal manures - slurries - nitrification - denitrification - nitrogen - prices - price formation - costs
    Het Praktijkonderzoek Varkenshouderij heeft een handleiding opgesteld voor het ontwerp van biologische stikstofvetwijderingsprocessen voor mestvloeistoffen. Belangrijkste voorwaarde voor het proces is een beluchtingstijd van tien dagen bij een minimale vloeistoftemperatuur van 10°C
    Kan mest scheiden economisch rendabel zijn?
    Kaa, C. van der; Verdoes, N. - \ 1997
    Praktijkonderzoek varkenshouderij 11 (1997)3. - ISSN 1382-0346 - p. 24 - 25.
    mest - vloeibare meststoffen - rundveedrijfmest - varkens - dierlijke meststoffen - marketing - toevoegingen - stalmest - kunstmeststoffen - samenstelling - onderzoek - proeven - productiviteit - rentabiliteit - dierhouderij - mestoverschotten - mestverwerking - manures - liquid manures - cattle slurry - pigs - animal manures - marketing - additives - farmyard manure - fertilizers - composition - research - trials - productivity - profitability - animal husbandry - manure surpluses - manure treatment
    Met de aanpassing van de BGDM-normen (Besluit Gebruik Dierlijke Meststoffen) begin dit jaar en de invoering van MINAS vanaf 1998, kan het scheiden van mest in een dikke en dunne waterige mestfractie aantrekkelijker worden.
    Ontwikkelingen op milieugebied in Sterksel
    Beurskens-Voermans, M. ; Kaa, C. van der - \ 1996
    Praktijkonderzoek varkenshouderij 10 (1996)6. - ISSN 1382-0346 - p. 7 - 7.
    luchtverontreiniging - ammoniak - rundveedrijfmest - emissie - vloeibare meststoffen - mest - biggen - zeugen - vervluchtiging - mestoverschotten - mestverwerking - air pollution - ammonia - cattle slurry - emission - liquid manures - manures - piglets - sows - volatilization - manure surpluses - manure treatment
    Het afgelopen jaar is op het Varkensproefbedrijf te Sterksel onderzoek verricht naar het effect van het koelen van de toplaag van de mest op de ammoniakemissie bij kraamzeugen en gespeende biggen.
    Biological sulphide oxidation in a fed-batch reactor.
    Janssen, A.J.H. ; Sleyster, R. ; Kaa, C. van der; Jochemsen, A. ; Bontsema, J. ; Lettinga, G. - \ 1995
    Biotechnology and Bioengineering 47 (1995). - ISSN 0006-3592 - p. 327 - 333.
    anaërobe behandeling - verwijdering - rioolwater - sulfiden - zwavel - afvalwater - afvalwaterbehandeling - waterzuivering - anaerobic treatment - removal - sewage - sulfides - sulfur - waste water - waste water treatment - water treatment
    Auteurs voor Nederland voor European Observatory on National Family Policies.
    Presvelou, C. ; Hoog, C. de; Kaa, D.J. van der; Brekel, J.C. van den - \ 1994
    In: National Family Policies in the Member States of the European Union in 1992 and 1993, Synthesis Report / Dumon, W., Nuelant, T.,
    Relatievormen in Nederland, 1984 ( ORIN ) [Ways of cohabitation in the Netherlands, 1984]
    Klijzing, F.K.H. ; Kaa, D.J. ; Keilman, N.W. ; Moors, H.G. ; Kuijsten, A.C. ; Leeuwen, L.Th. van; Akker, P.A.M. - \ 1989
    Landbouwuniversiteit Wageningen
    demography - family life - living conditions - sociology of the family
    Background and characteristics of various ways of cohabitation in the Netherlands. R's nr. of marriages / having lived together with partner before marriage / nr. of times of having lived together with a partner / having an intimate relationship without living together / plans about going to live together or marriage / preferred way of cohabitation / nr. of own and or adopted children / nr. of children living with r at present and in the past / fertility and ( reasons of ) infertility / preferred nr. of children / used birth control methods at present and in the past / preferred age for men as well as for women to get the first child / age at which a man or a woman are considered too old to get a child / discussions with partner ( before or after marriage ) about having children / residence where r spent childhood / profession of father / whether r's mother had a ( part-time ) job during r's childhood / profession of mother / being raised according to christian or ideological principles / r's religious or ideological denomination at present / whether r joined the forces / temporarily leaving of parental home during childhood / age at which r left parental home for the first time / living conditions after having left the parental home / marital status, household composition and its changes / reasons for changes in way of cohabitation / r's education, work- experiences and source( s ) of income / education, work-situation and source( s ) of income of partner / housing situation and reasons for changes in housing situation / importance of religion in personal life / evaluation of personal and social consequences of an intimate relationship / various aspects of an intimate relationship: emotional meaning, anticipation of a divorce, financial responsibilities, responsibilities for housekeeping, meaning and aspects of parenthood / remarriage / having children and not being married / chances for lesbian couples to have children / sterilization / working outdoors of married women with children / homosexual life-style / extra-marital affairs / calling parents by their first name / being a virgin before marriage / being voluntarily childless / abortion / reasons for going to marry in the near future / whether getting children is a reason for marriage / important reasons for starting to live together or for marriage / causes of tensions in relationship / "quality" of relationship / thinking about separating from present partner / most important reasons for divorce or leaving the partner with which r was living together / favourite type of ( intimate ) relationship, lifestyle. Background variables: basic characteristics/ residence/ housing situation/ household characteristics/ occupation/employment/ income/capital assets/ education/ religion
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