Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Microbial lifestyle engineering
    Kampers, Linde F.C. - \ 2020
    Wageningen University. Promotor(en): V.A.P. Martins dos Santos, co-promotor(en): P.J. Schaap. - Wageningen : Wageningen University - ISBN 9789463953245 - 228
    In silico-guided engineering of Pseudomonas putida towards growth under micro-oxic conditions
    Kampers, Linde F.C. ; Heck, Ruben G.A. van; Donati, Stefano ; Saccenti, Edoardo ; Volkers, Rita J.M. ; Schaap, Peter J. ; Suarez-Diez, Maria ; Nikel, Pablo I. ; Martins Dos Santos, Vitor A.P. - \ 2019
    Microbial Cell Factories 18 (2019)1. - ISSN 1475-2859 - 1 p.
    Anaerobiosis - Comparative genomics - Constraint-based metabolic modelling - Domainome analysis - Microbial physiology - Synthetic biology

    BACKGROUND: Pseudomonas putida is a metabolically versatile, genetically accessible, and stress-robust species with outstanding potential to be used as a workhorse for industrial applications. While industry recognises the importance of robustness under micro-oxic conditions for a stable production process, the obligate aerobic nature of P. putida, attributed to its inability to produce sufficient ATP and maintain its redox balance without molecular oxygen, severely limits its use for biotechnology applications. RESULTS: Here, a combination of genome-scale metabolic modelling and comparative genomics is used to pinpoint essential [Formula: see text]-dependent processes. These explain the inability of the strain to grow under anoxic conditions: a deficient ATP generation and an inability to synthesize essential metabolites. Based on this, several P. putida recombinant strains were constructed harbouring acetate kinase from Escherichia coli for ATP production, and a class I dihydroorotate dehydrogenase and a class III anaerobic ribonucleotide triphosphate reductase from Lactobacillus lactis for the synthesis of essential metabolites. Initial computational designs were fine-tuned by means of adaptive laboratory evolution. CONCLUSIONS: We demonstrated the value of combining in silico approaches, experimental validation and adaptive laboratory evolution for microbial design by making the strictly aerobic Pseudomonas putida able to grow under micro-oxic conditions.

    Pseudomonas putida KT2440 is HV1 certified, not GRAS
    Kampers, Linde F.C. ; Volkers, Rita J.M. ; Martins dos Santos, Vitor A.P. - \ 2019
    Microbial Biotechnology 12 (2019)5. - ISSN 1751-7907 - p. 845 - 848.

    Pseudomonas putida is rapidly becoming a workhorse for industrial production due to its metabolic versatility, genetic accessibility and stress-resistance properties. The P. putida strain KT2440 is often described as Generally Regarded as Safe, or GRAS, indicating the strain is safe to use as food additive. This description is incorrect. P. putida KT2440 is classified by the FDA as HV1 certified, indicating it is safe to use in a P1 or ML1 environment.

    Vaccination of carp against SVCV with an oral DNA vaccine or an insect cells-based subunit vaccine
    Embregts, C.W.E. ; Rigaudeau, D. ; Tacchi, L. ; Pijlman, G.P. ; Kampers, L. ; Veselý, T. ; Pokorová, D. ; Boudinot, P. ; Wiegertjes, G.F. ; Forlenza, M. - \ 2019
    Fish and Shellfish Immunology 85 (2019). - ISSN 1050-4648 - p. 66 - 77.
    Alginate encapsulation - Baculovirus - DNA vaccine - Insect cells - SVCV glycoprotein
    We recently reported on a successful vaccine for carp against SVCV based on the intramuscular injection of a DNA plasmid encoding the SVCV glycoprotein (SVCV-G). This shows that the intramuscular (i.m.) route of vaccination is suitable to trigger protective responses against SVCV, and that the SVCV G-protein is a suitable vaccine antigen. Yet, despite the general success of DNA vaccines, especially against fish rhabdoviruses, their practical implementation still faces legislative as well as consumer's acceptance concerns. Furthermore, the i.m. route of plasmid administration is not easily combined with most of the current vaccination regimes largely based on intraperitoneal or immersion vaccination. For this reason, in the current study we evaluated possible alternatives to a DNA-based i.m. injectable vaccine using the SVCV-G protein as the vaccine antigen. To this end, we tested two parallel approaches: the first based on the optimization of an alginate encapsulation method for oral delivery of DNA and protein antigens; the second based on the baculovirus recombinant expression of transmembrane SVCV-G protein in insect cells, administered as whole-cell subunit vaccine through the oral and injection route. In addition, in the case of the oral DNA vaccine, we also investigated the potential benefits of the mucosal adjuvants Escherichia coli lymphotoxin subunit B (LTB). Despite the use of various vaccine types, doses, regimes, and administration routes, no protection was observed, contrary to the full protection obtained with our reference i.m. DNA vaccine. The limited protection observed under the various conditions used in this study, the nature of the host, of the pathogen, the type of vaccine and encapsulation method, will therefore be discussed in details to provide an outlook for future vaccination strategies against SVCV.
    ‘Let’s take the lead in sustainable agriculture’
    Kampers, F.W.H. - \ 2017
    'Laat Nederland het voortouw nemen voor duurzame landbouw'
    Kampers, F.W.H. - \ 2017
    Onderzoeksagenda voor beter voedselsysteem
    Kampers, F.W.H. - \ 2017
    Food transitions 2030 : How to achieve the transitions to a sustainable,affordable, trustworthy and high-quality food system in the next decade or two that will fulfil the needs of a diverse and growing world population
    Kampers, Frans W.H. ; Fresco, Louise O. - \ 2017
    Wageningen : Wageningen University & Research - 63
    De agrofoodsector heeft robots hard nodig : White paper
    Beulens, Adrie ; Henten, Eldert van; IJsselmuiden, Joris ; Jongebloed, Peter ; Kampers, Frans ; Kooistra, Lammert ; Kootstra, Gert ; Maas, Jelle ; Meijer-Michielsen, Desirée ; Nieuwenhuizen, Jeroen van den; Pekkeriet, Erik ; Zedde, Rick van der - \ 2015
    Wageningen : Wageningen UR - 17
    How nanotechnologies can enhance sustainability in the agrifood sector
    Kampers, Frans W.H. - \ 2014
    In: Nanotechnology for Sustainable Manufacturing / Rickerby, D., CRC Press - ISBN 9781482214826 - p. 59 - 77.

    Nanotechnologies provide a toolbox full of new and highly precise tools that can be used in a wide range of application fields. Since biological systems are primarily made up of nanostructured materials and the processes in biology largely depend on functionality arising at the nano level, it is not surprising that there are many opportunities for these new tools in the different fields that directly rely on biological processes. The agrifood sector is one of these application fields for nanotechnologies (Joseph and Morrison 2006; Kuzma and Verhage 2006; Chaudhry et al. 2010; Frewer et al. 2011). However, as with all new technologies, there are also critical notes (ETC Group 2004; Friends of the Earth 2008) pointing out that there are also risks associated with these applications, and these will be discussed in Section 4.6. Most people agree that opportunities are enormous and the potential for benefits to consumers, society, and the environment is large. Here, the potential for improving the sustainability of the agrifood sector is explored.

    The views of experts and the public regarding societal preferences for innovation in nanotechnology
    Gupta, N. - \ 2013
    Wageningen University. Promotor(en): Lynn Frewer, co-promotor(en): Arnout Fischer; Frans Kampers. - Wageningen : Wageningen UR - ISBN 9789461737625 - 175
    nanotechnologie - aanvaardbaarheid - innovatie adoptie - innovaties - deskundigen - consumenten - attitudes - opinies - voedselproductie - consumentengedrag - voedselacceptatie - nanotechnology - acceptability - innovation adoption - innovations - experts - consumers - attitudes - opinions - food production - consumer behaviour - food acceptability
    Nanotechnology represents an emerging technology with varied application areas. It has been identified as the next scientific breakthrough with potential for positive impacts for society. The development and application of emerging technologies has been shown to be contingent upon societal responses to those technologies and their applications. Socio-psychological factors will potentially influence societal responses to nanotechnology, and play an important role in its development and commercialisation. The views of both experts and the general public regarding societal preferences for innovations in nanotechnology will be important in identifying which applications will be commercialised. If expert views regarding the determinants of societal acceptability do not align with those held by the general public, applications that consumers will reject may be introduced early in the commercialisation trajectory and focus public opinion on the negative aspects of nanotechnology. Conversely, applications which are acceptable to consumers may never be commercialised if experts perceive that consumers are likely to reject them. This thesis identifies and describes factors influencing societal response to nanotechnology by incorporating views from experts and the general public. Three research approaches were utilised. The first focussed on reviewing previous scientific research into the socio-psychological factors that influence public acceptance of new technologies. The second focussed on identifying and quantifying expert views on the factors that will influence societal acceptance or rejection of nanotechnology. The third tested whether the different factors do, in fact, drive public opinion.
    Approaches to the safety assessment of engineered nanomaterials (ENM) in food
    Cockburn, A. ; Bradford, R. ; Buck, N. ; Kampers, F.W.H. - \ 2012
    Food and Chemical Toxicology 50 (2012)6. - ISSN 0278-6915 - p. 2224 - 2242.
    vitro digestion models - nanoparticle uptake - protein corona - polymeric nanoparticles - gastrointestinal-tract - silica nanoparticles - particle-size - in-vivo - toxicity - bioaccessibility
    A systematic, tiered approach to assess the safety of engineered nanomaterials (ENMs) in foods is presented. The ENM is first compared to its non-nano form counterpart to determine if ENM-specific assessment is required. Of highest concern from a toxicological perspective are ENMs which have potential for systemic translocation, are insoluble or only partially soluble over time or are particulate and bio-persistent. Where ENM-specific assessment is triggered. Tier 1 screening considers the potential for translocation across biological barriers, cytotoxicity, generation of reactive oxygen species, inflammatory response, genotoxicity and general toxicity. In silica and in vitro studies, together with a sub-acute repeat-dose rodent study, could be considered for this phase. Tier 2 hazard characterisation is based on a sentinel 90-day rodent study with an extended range of endpoints, additional parameters being investigated case-by-case. Physicochemical characterisation should be performed in a range of food and biological matrices. A default assumption of 100% bioavailability of the ENM provides a 'worst case' exposure scenario, which could be refined as additional data become available. The safety testing strategy is considered applicable to variations in ENM size within the nanoscale and to new generations of ENM.
    Europees onderzoek gestart voor gerobotiseerd en flexibel verpakken van voeding
    Pekkeriet, E.J. ; Kampers, F.W.H. - \ 2012
    Wageningen : Wageningen UR Glastuinbouw
    'Doorbreek de stilte rond nanotechnologie in voedsel'
    Kampers, F.W.H. - \ 2012
    Kennis Online 9 (2012)december. - p. 4 - 5.
    nanotechnologie - voedseltechnologie - voedselveiligheid - organische scheikunde - toxiciteit - bionanotechnologie - houding van consumenten - nanotechnology - food technology - food safety - organic chemistry - toxicity - bionanotechnology - consumer attitudes
    Levensmiddelenbedrijven vertellen liever niet dat hun eten uit de fabriek komt. Gevaarlijk, zegt Frans Kampers van de Agrotechnolgy & Food Sciences Group. Want als er iets misgaat, zal een ongeïnformeerde consument helemaal in paniek raken.
    Einstein zou het nu in Nederland niet redden'
    Kampers, F.W.H. - \ 2011
    Kennis Online 8 (2011)dec12.
    nanotechnologie - wetenschap - onderzoek - nanotechnology - science - research
    Nanotechnologie is nog volop in ontwikkeling. De overheid zou daar rekening mee moeten houden, door ook te investeren in fundamenteel onderzoek.
    Using nanoparticles in agricultural and food diagnostics
    Posthuma-Trumpie, G.A. ; Amerongen, A. van - \ 2011
    In: Nanotechnology in the agri-food sector : implications for the future / Frewer, L.J., Norde, W., Fischer, A., Kampers, F., Weinheim : Wiley-VCH - ISBN 9783527634798 - p. 75 - 87.
    Food functionality and the physics of bionanotechnology: some examples and challenges
    Linden, E. van der - \ 2011
    In: Nanotechnology in the Agri-Food Sector: Implications for the future / Frewer, L.J., Norde, W., Fischer, A.R.H., Kampers, F.W.H., Wiley-VCH - ISBN 9783527330607 - p. 127 - 148.
    Evolving Best Practice in Governance Policy - Developing Consumer Confidence in Risk Analysis Applied to Emerging Technologies
    Marvin, H.J.P. ; Bouwmeester, H. ; Kleter, G.A. ; Frewer, L.J. ; Wentholt, M.T.A. - \ 2011
    In: Nanotechnology in the Agri-Food Sector. Implications for the Future / Frewer, L.J., Norde, W., Fischer, A.R.H., Kampers, F.W.H., Weinheim, Germany : Wiley-VCH - ISBN 9783527330607 - p. 283 - 300.
    Nanotechnology in the Agri-Food Sector. Implications for the Future
    Frewer, L.J. ; Norde, W. ; Fischer, A.R.H. ; Kampers, F.W.H. - \ 2011
    Weinheim, Germany : Wiley-VCH - ISBN 9783527330607 - 328
    nanotechnologie - voedselbiotechnologie - toxiciteit - nanotechnology - food biotechnology - toxicity
    Public Engagement with Emerging Issues in Agri-Food Nanotechnology
    Frewer, L.J. ; Fischer, A.R.H. ; Rowe, G. - \ 2011
    In: Nanotechnology in the Agri-Food Sector. Implications for the Future / Frewer, L.J., Norde, W., Fischer, A.R.H., Kampers, F.W.H., Weinheim, Germany : Wiley-VCH - ISBN 9783527330607 - p. 257 - 270.
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