Surface-enhanced laser desorption/ionisation time-of-flight mass spectrometry: A tool to predict pork quality
Marcos, B. ; Gou, P. ; Guardia, M.D. ; Hortos, M. ; Colleo, M. ; Mach Casellas, N. ; Pas, M.F.W. te; Keuning, E. ; Kruijt, L. ; Tibau, J. ; Gispert, M. ; Arnau, J. - \ 2013
Meat Science 95 (2013)3. - ISSN 0309-1740 - p. 688 - 693.
dry-cured hams - seldi-tof-ms - boar taint - sensory characteristics - meat quality - androstenone - pigs - fat
Expression of water soluble proteins of fresh pork Longissimus thoracis from 4 pure breed pigs (Duroc, Large White, Landrace, and Pietrain) was studied to identify candidate protein markers for meat quality. Surface-enhanced laser desorption/ionisation time-of-flight mass spectrometry (SELDI-TOF-MS) was used to obtain the soluble protein profiles of Longissimus thoracis muscles. The pure breeds showed differences among the studied meat quality traits (pH(u), drip loss, androstenone, marbling, intramuscular fat, texture, and moisture), but no significant differences were detected in sensory analysis. Associations between protein peaks obtained with SELDI-TOF-MS and meat quality traits, mainly water holding capacity, texture and skatole were observed. Of these peaks, a total of 10 peaks from CM10 array and 6 peaks from Q10 array were candidate soluble protein markers for pork loin quality. The developed models explained a limited proportion of the variability, however they point out interesting relationships between protein expression and meat quality. (C) 2012 Elsevier Ltd. All rights reserved.
|Grote afnemers willen onderzoek naar misstanden vleesverwerkers; Problemen in de vleessector kunnen voortbestaan dankzij matig toezicht (interview met o.a. M.H. Zwietering)
Zwietering, Marcel - \ 2013
Identification of proteomic biomarkers in M. Longissimus dorsi as potential predictors of pork quality
Pas, M.F.W. te; Kruijt, L. ; Pierzchala, M. ; Crump, R.E. ; Boeren, S. ; Keuning, E. ; Hoving-Bolink, A.H. ; Hortós, M. ; Gispert, M. ; Arnau, J. ; Diestre, A. ; Mulder, H.A. - \ 2013
Meat Science 95 (2013)3. - ISSN 0309-1740 - p. 679 - 687.
meat quality - sample preparation - muscles - values - performance - expression - slaughter - traits - system - breeds
Meat quality traits have low heritability and large environmental influences. To predict, improve and manage meat quality, proteomic biomarkers are superior to genetic markers. The objectives of this research were (1) to find associations between proteome profiles of longissimus muscle at slaughter and meat quality SELDI-TOF proteome profiles of 142 LargeWhite × Duroc cross pigs showed relationships among peaks or combinations of peaks and meat quality traits with highest significance for drip loss and ultimate pH. Calculated accuracies of prediction of traits ranged from 20 up to 80%. Differentially expressed proteins related to drip loss and ultimate pH were identified by NanoLC-FTMSMS. The proteins highlight biological mechanisms that may explain how these traits develop biologically and how they are related to each other
Analysis of raw hams using SELDI-TOF-MS to predict the final quality of dry-cured hams
Marcos, B. ; Gou, P. ; Serra, X. ; Guardia, M.D. ; Zhen, Z.Y. ; Hortos, M. ; Mach Casellas, N. ; Pas, M.F.W. te; Keuning, E. ; Kruijt, L. ; Font I Furnols, M. ; Arnau, J. - \ 2013
Meat Science 93 (2013)2. - ISSN 0309-1740 - p. 233 - 239.
sensory characteristics - defective texture - proteome analysis - meat quality - ph - muscles - traits - duroc - sex
The relationship between protein profiles of Gluteus medius (GM) muscles of raw hams obtained from 4 pure breed pigs (Duroc, Large White, Landrace, and Piétrain) with the final quality of the Semimembranosus and Biceps femoris muscles of dry-cured hams was investigated. As expected, Duroc hams showed higher levels of marbling and intramuscular fat content than the other breeds. Piétrain hams were the leanest and most conformed, and presented the lowest salt content in dry-cured hams. Even if differences in the quality traits (colour, water activity, texture, composition, intramuscular fat, and marbling) of dry-cured hams were observed among the studied breeds, only small differences in the sensory attributes were detected. Surface-enhanced laser desorption/ionisation time-of-flight mass spectrometry (SELDI-TOF-MS) was used to obtain the soluble protein profiles of GM muscles. Some associations between protein peaks obtained with SELDI-TOF-MS and quality traits, mainly colour (b*) and texture (F(0), Y(2), Y(90)) were observed. Candidate protein markers for the quality of processed dry-cured hams were identified.
Functional analysis of inter-individual transcriptome differential expression in pig longissimus muscle
Zhao, S. ; Hulsegge, B. ; Harders, F.L. ; Bossers, R. ; Keuning, E. ; Hoekman, A.J.W. ; Hoving-Bolink, A.H. ; Pas, M.F.W. te - \ 2013
Journal of Animal Breeding and Genetics 130 (2013)1. - ISSN 0931-2668 - p. 72 - 78.
meat quality - microarrays - discovery - traits - fibers - gene - pork
Selection of pigs for increased meat production or improved meat quality changes muscle mass and muscle composition. This will be related to transcriptome expression profile changes in muscle tissue, generating inter-individual differences. This study investigated the differentially expressed genes in the transcriptome profiles of the longissimus muscle of 75 Large White–Duroc cross sows and castrates. The use of a common reference design enabled to investigate the inter-individual transcriptome expression profile differences between the animals as compared with the means of all animals. The aim of the study was to identify the biological processes related to these inter-individual differences. It was expected that these processes underlie the selection effects. In total, 908 transcripts were differentially expressed. Among them, 762 were mainly downregulated and 146 were mainly upregulated. Gene Ontology and Pathways analyses indicated that the differentially expressed genes belong to three groups of processes involved in protein synthesis and amino acid–protein metabolism, energy metabolism and muscle-specific structure and activity processes. Comparing the functional biological analysis results with previously reported data suggested that the protein synthesis, energy metabolism and muscle-specific structure would contribute to meat production and the meat quality
|Proteomics biomarkers for pork quality
Pas, M.F.W. te; Pierzchala, M. ; Keuning, E. ; Crump, R.E. ; Boeren, J.A. ; Hortos, M. ; Gisbert, M. ; Diestre, A. ; Kruijt, L. - \ 2012
In: Farm animal proteomics / Rodrigues, P., Eckersall, D., de |Almeida, A., Wageningen : Wageningen Academic Publishers - ISBN 9789086861958 - p. 194 - 198.
|Ecosysteemdiensten in de praktijk van duurzaam bodembeheer. Deel II
Keuning, S. ; Smit, A. - \ 2011
Bodem 21 (2011)nummer 1. - ISSN 0925-1650 - p. 22 - 24.
ecosysteemdiensten - bodembeheer - bodemkwaliteit - ecosystem services - soil management - soil quality
Ecosysteemdiensten trekken steeds meer aandacht. Internationaal zijn ze door de VN op de agenda gezet om het bewustzijn te vergroten dat biodiversiteit en de ecosystemen op aarde er toe doen en de basis vormen voor ons welzijn en onze economische welvaart. Maar ook in onze bodemwereld broeit het begrip en worden ecosysteemdiensten al getipt als de nieuwe onderlegger voor duurzaam bodembeheer. Tenminste als we RBB en BoBI hierin ook een plek kunnen geven. Maar met zoveel nieuw jargon gaat het menigeen duizelen, zeker als die begrippen ook nog op verschillende manieren worden gebruikt en ingevuld. De hoogste tijd dus om een aantal zaken eens op een rijtje zetten.
|Surface-enhanced laser desorption/ionisation time-of-flight mass spectrometry: a fast method to assess pork quality
Marcos, B. ; Gou, P. ; Guàrdia, M.D. ; Colleo, M. ; Hortós, M. ; Mach Casellas, N. ; Pas, M.F.W. te; Keuning, E. ; Kruijt, L. ; Hoving-Bolink, A.H. ; Gispert, M. ; Arnau, J. - \ 2011
Proteome Profiles of Longissimus and Biceps femoris Porcine Muscles Related to Exercise and Resting
Pas, M.F.W. te; Keuning, E. ; Wiel, D.F.M. van de; Young, J.F. ; Oksbjerg, N. ; Kruijt, L. - \ 2011
Journal of Life Sciences 5 (2011)8. - ISSN 1934-7391 - p. 598 - 608.
Exercise affects muscle metabolism and composition in the untrained muscles. The proteome of muscle tissue will be affected by exercise and resting. This is of economic importance for pork quality where transportation relates to exercise of untrained muscles. Rest reverses exercise effects. The objective of this research was to develop potential protein biomarkers that predict the optimal resting time after exercise related to optimal pork quality. Ten litters of four female pigs were within litter allocated to the four treatment groups: exercise by running on a treadmill for 27 minutes followed by rest for 0, 1, or 3 h; control pigs without exercise. Proteome profiles and biochemical traits measuring energy metabolism and meat quality traits expected to be related to exercise were determined in the Longissimus and the Biceps femoris of the pigs. The results indicated associations between protein abundances in muscles and exercise, resting, and biochemical traits.
Ecosysteemdiensten in de praktijk van duurzaam bodembeheer
Keuning, S. ; Smit, A. - \ 2010
Bodem 2010 (2010)6. - ISSN 0925-1650 - p. 11 - 13.
bodembeheer - natuurbescherming - economische analyse - ecosysteemdiensten - bodemecologie - soil management - nature conservation - economic analysis - ecosystem services - soil ecology
Ecosysteemdiensten - meestal gedefinieerd als de voordelen die de mens geniet van ecosystemen - combineert twee werelden: milieu en economie. Het concept bestaat inmiddels al meer dan twintig jaar, maar was tot dusver nog maar weinig bekend. Dat is nu aan het veranderen. Wat hebben ecosysteemdiensten te bieden? Is het een tijdelijke hype of begin van een nieuw tijdperk in het milieudenken? En wat betekent dat dan voor het bodembeheer?
Ecosysteemdiensten in de praktijk van duurzaam bodembeheer - Spoor Taal en Beeld
Keuning, S. ; Smit, A. - \ 2010
Groningen : Bioclear BV - 51
ecosysteemdiensten - bodembeheer - duurzaamheid (sustainability) - nederland - ecosystem services - soil management - sustainability - netherlands
Dit rapport beschrijft de resultaten van een deelproject van "Ecosysteemdiensten in de praktijk van duurzaam bodembeheer" en wel het onderdeel "taal en beeld". Het gaat hier om de ontwikkeling van taal en beelden rond het nog jonge begrip ecosysteemdiensten. Aanleiding van het project is de wens van VROM om grondeigenaren en beheerders te faciliteren bij het formuleren van ambities met betrekking tot bodemkwaliteit en bodemgebruik en de daarmee samenhangende ecosysteemdiensten tijdens het opstellen van nota's voor bodembeheer.
Longissimus muscle transcriptome profiles related to carcass and meat quality traits in fresh meat Pietrain carcasses
Pas, M.F.W. te; Keuning, E. ; Hulsegge, B. ; Hoving-Bolink, A.H. ; Evans, G. ; Mulder, H.A. - \ 2010
Journal of Animal Science 88 (2010)12. - ISSN 0021-8812 - p. 4044 - 4055.
skeletal-muscle - expression profiles - intramuscular fat - protein-synthesis - pigs - genes - tissue - kegg - hypertrophy - genomes
High quality pork is consumed as fresh meat while other carcasses are used in the processing industry. Meat quality is determined measuring technical muscle parameters. The objective of this research was to investigate the molecular regulatory mechanisms underlying meat quality differences of pork originating from genetically different Pietrain boars. Pietrain boars were approved for high meat quality using a DNA marker panel. Other Pietrain boars were indicated as average. Both groups produced litters in similar Pietrain sows. The longissimus muscles were sampled from nine carcasses produced by approved boars and eight carcasses of average boars. RNA was isolated and an equal portion of each sample was pooled to make a reference sample representing the mean of all samples. Each sample was hybridized on microarrays against the reference in duplicate using a dye swaps design. After normalization and subtraction of two times the background only genes expressed in at least five carcasses were analyzed. For all analyses the mean of the M-values relative to the reference, i.e. fold change, were used. Sixteen genes showed significant linear or quadratic associations between gene expression levels and meat color (Minolta a* value, Minolta L* value, reflection, pH 24 h) after Bonferroni correction. All these genes had expression levels similar to the reference in all carcasses. Studying association between gene expression levels and meat quality using only genes with expression statistically differing from the reference in at least five carcasses revealed 29 more genes associating with the technological meat quality parameters, again with meat color as a main trait. These associations were not significant after Bonferroni correction and explained less of the phenotypic variation in the traits. Bioinformatics analyses with DAVID using the list of genes with more than two-fold changed expression level revealed that these genes were mainly found in muscle-specific processes, protein complexes, and oxygen transport, and located to muscle-specific cellular localizations. Pathway analysis using the KEGG database revealed pathways related to protein metabolism, cellular proliferation, signaling, and adipose development differing between the two groups of carcasses. Approved meat carcasses showed less variation in gene expression. The results highlight biological molecular mechanisms underlying the differences between the high meat quality approved and average boars
Comparative proteomic profiling of 2 muscles from 5 different pure pig breeds using surface-enchanced laser desorption/ionization time-of-flight proteomics technology
Mach Casellas, N. ; Keuning, E. ; Kruijt, L. ; Hortós, M. ; Arnau, J. ; Pas, M.F.W. te - \ 2010
Journal of Animal Science 88 (2010)4. - ISSN 0021-8812 - p. 1522 - 1534.
pietrain-sired pigs - seldi-tof-ms - meat quality - rearing environment - growth - duroc - mass - semimembranosus - proteins - lumborum
The objectives of this study were to evaluate the influence of different pure pig breeds and muscle types on the expression of muscle proteins, as well as their interactions, and second, to find biomarkers for breed and muscle types. A total of 126 male pigs, including 43 Landrace, 21 Duroc, 43 Large White, 13 Pietrain, and 6 Belgian Landrace, were slaughtered at the age of 174 ± 6 d. Samples from the semimembranosus muscle (SM) and LM were collected 24 h postmortem. Proteomic spectra were generated on an anion exchanger (Q10), a cation exchanger (CM10), and on immobilized metal affinity capture (IMAC30) ProteinChip arrays and analyzed using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry ProteinChip techniques. Breed and muscle type did not affect the number of peaks per spectrum but, interestingly, affected the average intensity of the peaks. Of these peaks, a total of 4 proved to be potential protein biomarkers to differentiate LM or SM muscles, and 2 to classify specific breed types. Additionally, several peaks influenced by the interaction between muscle and breed types could correctly classify pig muscles according to their breed. Further studies need to be carried out to validate and identify these potential protein biomarkers for breed and muscle types in finishing pigs
Biomarker development for recovery from stress in pig muscles
Pas, M.F.W. te; Keuning, E. ; Kruijt, L. ; Wiel, D.F.M. van de; Young, J.F. ; Oksbjerg, N. - \ 2009
In: Book of Abstracts of the 60th Annual Meeting of the European Association for Animal Production EAAP, Barcelona, Spain, 24-27 August 2009. - Wageningen Academic Publishers - ISBN 9789086861217 - p. 279 - 279.
Pork quality is negatively influenced by stress due to pre-slaughter exhaustion of energy metabolism components in muscle fibres. Changes in the proteome are expected due to related protein breakdown. Thus proteome profiles may indicate the status of stress and recovery from stress of muscle tissue. Therefore, pigs were exercised on a treadmill for 30 minutes and slaughtered immediately or after a recovery period of 1 or 3 hours. Control pigs were treated as regular slaughter pigs. Each group consisted of 10 pigs. The longissimus and biceps femoris muscles were sampled. Proteomics profiles were determined for each individual sample using the SELDI-TOF-MS equipment and 3 different array types analyzing positively or negatively charged proteins, or phosphorylated proteins, respectively. Analyses were performed with the software provided with the equipment. The analyses compared the expression levels of individual peaks for each of the animals per muscle and per treatment group. All proteomics experiments were done in duplicate. Each proteomics experiment was individually analyzed and only peaks that appeared in both analyses were considered significant. The first results indicate that the changes of the expression of a number of peaks may be related to stress and recovery of stress. Compared to non-stressed control pigs the expression of these proteins is either decreased or elevated after stress and the changes are reversed during the recovery period. However, peaks differ in the timing of this process. Therefore, at present we cannot conclude on the association between peak expression profile and stress recovery. Further analyses on the repeatability of the analyses are ongoing. Finally, it may be necessary to associate the proteomics expression profiles with physiological indicators of muscle response to stress before conclusions about possible biomarkers can be done.
Biomarker development for meat quality in pork production chains for Spanish dry cured ham using proteomics technology
Pas, M.F.W. te; Keuning, E. ; Kruijt, L. ; Hortós, M. ; Diestre, A. ; Evans, G.J. ; Hoving-Bolink, A.H. ; Hoekman, A.J.W. - \ 2009
In: Book of Abstracts of the 60th Annual Meeting of the European Association for Animal Production EAAP, Barcelona, Spain, 24-27 August 2009. - Wageningen Academic Publishers - ISBN 9789086861217 - p. 33 - 33.
Improvement of the meat quality of pork is a major aim in pork production. Special products such as Spanish dry cured ham are produced from lines bred and managed especially for this aim. Establishing a standard high quality of the hams would increase profitability of this sector. Since the proteome of muscle tissue composes a major component of meat and changes in the proteome are related to meat quality we investigated the relationship between proteome profiles and meat quality traits. The longissimus muscle was sampled in 2 batches of pigs bred with a terminal Duroc boar that were managed with the aim of increasing intramuscular fat content for Spanish dry cured ham production. Each batch (N=70) consisted of pigs from 2 independent producers. The determined meat quality parameters included: drip loss, pH at 24 h, and IMF (measured in the gluteus medius). Furthermore several carcass parameters were measured including lean meat percentage and loin34FOM. Meat quality parameters did not differ between batches or producers. The proteome profiles of all samples were investigated with SELDI-TOF-MS technology using 3 different array types. Statistics were performed using the software provided with the equipment and with the Biomarker Patterns software. The results were analyzed for each batch independently and for the 2 batches taken together. To exclude false positives, only peaks that associated in all 3 analyses were regarded positive. We show statistically significant associations between a number of peaks indicated by the mass of the protein) and the meat quality traits mentioned above. The peaks may be composed of one or more proteins that could be used as biomarkers. Presently, experiments are ongoing to identify the proteins of the peaks that have the best associations. Biomarker tests will be developed based on these proteins.
Comparative proteomic profiling of two muscles from five divergent pig breeds using SELDI-TOF proteomics technology
Mach Casellas, N. ; Keuning, E. ; Kruijt, L. ; Hortós, M. ; Arnau, J. ; Pas, M.F.W. te - \ 2009
In: Book of Abstracts of the 60th Annual Meeting of the European Association for Animal Production EAAP, Barcelona, Spain, 24-27 August 2009. - Wageningen Academic Publishers - ISBN 9789086861217 - p. 34 - 34.
The aim of this study was to identify biomarkers for breed and muscle type through a proteome differential analysis. Samples from the semimembranosus muscle (SM) and longissimus muscle (LM) were collected 24 h post-mortem from 79 male pigs (174±6 d) of the Landrace, Duroc, Large White, Pietrain and Belgian Landrace breeds, following a 2 x 5 factorial design. The Surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry analysis was performed using the Protein Chip System Series 4000 equipment. The CM10 ProteinChip® array type and binding buffer pH5 containing 0.1% Triton was chosen. Data was normalized and corrected for background. Peak intensity was log-transformed. The number of peaks (36.33±0.87) was not affected by breed and muscle type. However, the average intensity of peaks was affected (P
|Behaviour and welfare of semi-wild Scottish highland cattle in Nature-reserves.
Koene, P. ; Ardesch, J. ; Lycklema, R. ; Keuning, B. - \ 1996
In: Proc. 30th Int. Congress Int. Soc. for Applied Ethology, I.J.H. Duncan et al. (eds.), Guelph, Canada (1996) 1 p
Urinebrandplekken in grasland : resultaten preventie-onderzoek in 1984 en 1985 op ROC Cranendonck
Keuning, J.A. ; Groenwold, J. - \ 1993
Wageningen : CABO-DLO (Verslag / CABO-DLO 168) - 18
ammoniak - oogstschade - kunstmeststoffen - graslanden - guanine - hippurinezuur - hypoxanthinen - urine - ammonia - crop damage - fertilizers - grasslands - guanine - hippuric acid - hypoxanthines - urine
Door goed graslandbeheer lage voerkosten op bedrijf Hylkema
Keuning, J.A. - \ 1993
Praktijkonderzoek / Praktijkonderzoek Rundvee, Schapen en Paarden (PR), Waiboerhoeve 6 (1993)2. - ISSN 0921-8874 - p. 12 - 14.
graslanden - melkvee - melkveehouderij - concentraten - voedingsrantsoenen - mineralen - boekhouding - rekeningen van landbouwbedrijf - boekhouding van landbouwbedrijf - grasslands - dairy cattle - dairy farming - concentrates - feed rations - minerals - accounting - farm accounts - farm accounting
Naast de bedrijfsvoering is veel aandacht besteed aan verbetering van het grasland en aan goed graslandbeheer. In dit artikel wordt besproken wat de effecten hiervan kunnen zijn op krachtvoerverbruik, voerkosten en beperking van de mineralentoevoer.
Mineralenvoorziening melkvee bij emissie-beperkende maatregelen
Keuning, J.A. - \ 1992
Praktijkonderzoek / Praktijkonderzoek Rundvee, Schapen en Paarden (PR), Waiboerhoeve 5 (1992)3. - ISSN 0921-8874 - p. 17 - 20.
voer - mineralen - melkvee - melkveehouderij - lucht - hygiëne - luchtverontreiniging - ammoniak - emissie - vervluchtiging - boekhouding - feeds - minerals - dairy cattle - dairy farming - air - hygiene - air pollution - ammonia - emission - volatilization - accounting
Bij het terugdringen van de overschotten wordt doorgaans de meeste aandacht besteed aan stikstof en fosfor. In mindere mate is dit het geval met kalium. Dit element is niet alleen van belang bij de groei van de gewassen maar speelt ook een rol bij de diergezondheid.