Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Greenhouse gas reporting for the LULUCFsector in the Netherlands : methodological background, update 2020
    Arets, E.J.M.M. ; Kolk, J.W.H. van der; Hengeveld, G.M. ; Lesschen, J.P. ; Kramer, H. ; Kuikman, P.J. ; Schelhaas, N.J. - \ 2020
    Wageningen : Statutory Research Tasks Unit for Nature & the Environment (WOt-technical report 168) - 127
    This report provides a complete methodological description and background information of the Dutch National System for Greenhouse gas reporting of the LULUCF sector. It provides detailed description of the methodologies, activity data and emission factors that were used. Each of the reporting categories Forest Land, Cropland, Grassland, Wetlands, Settlements, Other land and Harvested Wood Products are described in a separate chapter. Additionally it gives a table-by-table elaboration of the choices and motivations for filling the CRF tables for KP-LULUCF.
    Methods for one- and two–dimensional gas chromatography with flame ionization detection for identification of Mycobacterium tuberculosis in sputum
    Arslan, F.N. ; Kolk, A.H.J. ; Janssen, Hans-Gerd - \ 2019
    Journal of Chromatography. B, Analytical technologies in the biomedical and life sciences 1124 (2019). - ISSN 1570-0232 - p. 204 - 217.
    Two simplified methods based on manual thermally–assisted hydrolysis and methylation (THM) GC and GC × GC with flame ionization detection (FID) were developed for the detection of mycobacteria and Mycobacterium tuberculosis (MTB) in sputum. A central composite design was employed to optimize the THM derivatization conditions. For the detection of MTB the known mycobacterial markers tuberculostearic acid (TBSA) and hexacosanoic acid (C26), as well as three MTB specific markers, the mycocerosates, were evaluated. We found that the optimum conditions for THM release of TBSA and C26 differ from those for maximum release of the mycocerosates. Higher reagent volumes, higher temperatures and longer incubation increase the mycocerosates yield. Application of these conditions unfortunately resulted in unacceptable safety hazards. A GC × GC–FID method was developed that allowed accurate detection of mycocerosates even at poor conversion yields of the derivatization reaction. Using spiked sputum samples from non–TB patients, the detection limit of the method based on TBSA and C26 was found to be comparable to that of microscopy, i.e. 104–105 bacteria/mL sputum. To validate the new test, we compared the results we found for fifteen sputum samples from patients from South Africa suspected of having tuberculosis with those of culture, the gold standard method. Based on the presence of TBSA and C26, all eight microscopy and culture positive samples, and even two microscopy negative but culture positive samples were positive by THM–GC–FID. All five microscopy and culture negative sputum samples were also negative for THM–GC–FID, giving a specificity of 100%. Using GC × GC–FID we could detect mycocerosates, the specific markers for MTB in seven out of ten MTB culture positive sputum samples. The five culture negative cases were also negative for mycocerosates in manual THM–GC × GC–FID giving again 100% specificity. The results obtained indicate that the new methods hold great potential for the early diagnosis of TB in developing countries.
    Disturbance increases high tide travel distance of a roosting shorebird but only marginally affects daily energy expenditure
    Linssen, Hans ; De Pol, Martijn Van; Allen, Andrew M. ; Jans, Mitzi ; Ens, Bruno J. ; Krijgsveld, Karen L. ; Frauendorf, Magali ; Kolk, Henk Jan Van Der - \ 2019
    Avian Research 10 (2019)1. - ISSN 2053-7166
    Biologging - Disturbance - Haematopus ostralegus - High tide roost - Movement ecology - Shorebird

    Background: Anthropogenic disturbance can negatively affect an animal's energy budget by evoking movement responses. Existing research focuses mainly on immediate displacement as a disturbance effect, since this can be easily observed in the field. However, effects on movement over longer timescales are poorly examined and it is largely unknown if and to what extent they reflect immediate responses. Longer-term responses could for example be larger than immediate responses if birds, after disturbance, return to the original location and thereby travel twice the immediate disturbed distance. Methods: We combined GPS tracking data with observational data to quantify the effects of anthropogenic (air force and walkers) and non-anthropogenic disturbances on distances travelled by roosting Eurasian Oystercatchers (Haematopus ostralegus) during the non-breeding season. We compared immediate displacement after a disturbance with distance travelled during the entire high tide period (longer-term response), while accounting for environmental factors. Additionally, we calculated energy expenditure due to disturbance based on observed disturbance frequencies. Results: Disturbance resulted in an immediate displacement response of ~ 200 m (median). Air force disturbances tended to yield larger immediate responses than walker and, especially, than non-anthropogenic disturbances. Longer-term responses and immediate responses were approximately similar, suggesting that, over longer timescales, spatial disturbance effects in the study area remain confined to immediate effects. However, disturbances were infrequent (0.17 disturbances per bird per hour) and most disturbances were of natural origin (62%). Consequently, anthropogenic disturbance of roosting oystercatchers in the study area on average costs 0.08% of the daily energy expenditure. Conclusions: Our results suggest that immediate spatial responses to disturbance can be a useful proxy for spatial responses over longer timescales. Over the non-exhaustive range of conditions investigated, energetic consequences of spatial disturbance responses for an oystercatcher in the study area are marginal due to low disturbance levels.

    Greenhouse gas reporting of the LULUCF sector in the Netherlands : Methodological background, update 2019
    Arets, E.J.M.M. ; Kolk, J.W.H. van der; Hengeveld, G.M. ; Lesschen, J.P. ; Kramer, H. ; Kuikman, P.J. ; Schelhaas, M.J. - \ 2019
    Wageningen : Statutory Research Tasks Unit for Nature & the Environment (WOt technical report 146) - 111
    This report provides a complete methodological description and background information of the Dutch National System for Greenhouse Gas Reporting of the LULUCF sector. It provides detailed description of the methodologies, activity data and emission factors that were used. Each of the reporting categories Forest Land, Cropland, Grassland, Wetlands, Settlements, Other land and Harvested Wood Products are described in a separate chapter. Additionally it gives a table-by-table elaboration of the choices and motivations for filling the CRF tables for KP-LULUCF.
    Methodology for estimating emissions from agriculture in the Netherlands : Calculations of CH4, NH3, N2O, NOx, NMVOC, PM10, PM2.5 and CO2 with the National Emission Model for Agriculture (NEMA), Update 2019
    Lagerwerf, L.A. ; Bannink, A. ; Bruggen, C. van; Groenestein, C.M. ; Huijsmans, J.F.M. ; Kolk, J.W.H. van der; Luesink, H.H. ; Sluis, S.M. van der; Velthof, G.L. ; Vonk, J. - \ 2019
    Wageningen : Statutory Research Tasks Unit for Nature & the Environment (WOt technical report 148) - 215
    The National Emission Model for Agriculture (NEMA) is used to calculate emissions to air from agricultural activities in the Netherlands on a national scale. Emissions of ammonia (NH3) and other N compounds (NOx and N2O) are calculated for animal housing, manure storage, manure application and grazing using a flow model for total ammoniacal nitrogen (TAN). Emissions from the application of inorganic N fertilizer, compost and sewage sludge, cultivation of organic soils, crop residues, and ripening of crops are calculated as well. The NEMA is also used to estimate emissions of methane (CH4) from enteric fermentation and manure management, nonmethane volatile organic compounds (NMVOC) and particulate matter (PM) from manure management and agricultural soils, as well as for carbon dioxide (CO2) from liming. Emissions are calculated in accordance with the criteria of international guidelines and reported in an annual Informative Inventory Report (IIR; for air pollutants) and National Inventory Report (NIR; for greenhouse gases). This methodology report provides an outline of and describes the background to the calculation of emissions according to the NEMA.
    Methodology for estimating emissions from agriculture in the Netherlands – update 2018 : calculations of CH4, NH3, N2O, NOx, PM10, PM2.5 and CO2 with the National Emission Model for Agriculture (NEMA)
    Vonk, J. ; Sluis, S.M. van der; Bannink, A. ; Bruggen, C. van; Groenestein, C.M. ; Huijsmans, J.F.M. ; Kolk, J.W.H. van der; Lagerwerf, L.A. ; Luesink, H.H. ; Oude Voshaar, S.V. ; Velthof, G.L. - \ 2018
    Wageningen : Statutory Research Tasks Unit for Nature & the Environment (WOt-technical report 115) - 176
    The National Emission Model for Agriculture (NEMA) is used to calculate emissions to air from agricultural activities in the Netherlands on a national scale. Emissions of ammonia (NH3) and other N-compounds (NOx and N2O) are calculated from animal housing, manure storage, manure application and grazing using a Total Ammoniacal Nitrogen (TAN) flow model. Furthermore, emissions from application of inorganic N fertilizer, compost and sewage sludge, cultivation of organic soils, crop residues, and ripening of crops are calculated. NEMA is also used to estimate emissions of methane (CH4) from enteric fermentation and manure management, particulate matter (PM) from manure management and agricultural soils and carbon dioxide (CO2) from liming. Emissions are calculated in accordance with international guidance criteria and reported in an annual Informative Inventory Report (IIR; for air pollutants) and National Inventory Report (NIR; for greenhouse gases). This methodology report describes the outline and backgrounds of the emission calculations with NEMA.
    Greenhouse gas reporting for the LULUCF sector in the Netherlands: methodological background, update 2018
    Arets, E.J.M.M. ; Kolk, J.W.H. van der; Hengeveld, G.M. ; Lesschen, J.P. ; Kramer, H. ; Kuikman, P.J. ; Schelhaas, M.J. - \ 2018
    Wageningen : Statutory Research Tasks Unit for Nature & the Environment (WOt-technical report 113) - 101
    This report provides a complete methodological description and background information of the Dutch National System for Greenhouse gas reporting of the LULUCF sector. It provides detailed description of the methodologies, activity data and emission factors that were used. Each of the reporting categories Forest Land, Cropland, Grassland, Wetlands, Settlements, Other land and Harvested Wood Products are described in a separate chapter. Additionally it gives a table-by-table elaboration of the choices and motivations for filling the CRF tables for KP-LULUCF
    Greenhouse gas reporting for the LULUCF sector in the Netherlands : methodological background, update 2017
    Arets, E.J.M.M. ; Kolk, J.W.H. van der; Hengeveld, G.M. ; Lesschen, J.P. ; Kramer, H. ; Kuikman, P.J. ; Schelhaas, M.J. - \ 2017
    Wageningen : Statutory Research Tasks Unit for Nature & the Environment (WOt-technical report 95) - 89
    This report provides a complete methodological description and background information of the DutchNational System for Greenhouse gas Reporting of the LULUCF sector. It provides detailed description of themethodologies, activity data and emission factors that were used. Each of the reporting categories, ForestLand, Cropland, Grassland, Wetlands, Settlements, Other land and Harvested Wood Products are describedin a separate chapter. Additionally it gives a table-by-table elaboration of the choices and motivations forfilling the CRF tables for KP-LULUCF---Dit rapport geeft de methodologische achtergrondinformatie die gebruikt wordt binnen het nationale systeemom broeikasgasemissies voor de LULUCF-sector (landgebruik en bosbouw) te berekenen zoals die aan de VNKlimaatconventie (UNFCCC) en het Kyoto Protocol (KP) worden gerapporteerd. Het rapport geeftgedetailleerde beschrijvingen van de gehanteerde methodologie, gebruikte activiteitendata enemissiefactoren. De te rapporteren categorieën Bos (forest land), Bouwland (cropland), Grasland(grassland), Wetlands, Bebouwd gebied (Settlements), Ander land, en geoogste houtproducten worden perhoofdstuk beschreven. Daarnaast worden in een apart hoofdstuk de gebruikte aggregatiestappen gegevenom tot berekeningen voor het KP te komen en worden voor iedere KP-LULUCF CRF-tabel de gemaaktekeuzes om de tabel te vullen, beschreven en gemotiveerd
    Mild and Selective Protein Release of Cell Wall Deficient Microalgae with Pulsed Electric Field
    Lam, Gerard 't; Kolk, Jelmer A. van der; Chordia, Akshita ; Vermuë, Marian H. ; Olivieri, Giuseppe ; Eppink, Michel H.M. ; Wijffels, René H. - \ 2017
    ACS sustainable chemistry & engineering 5 (2017)7. - ISSN 2168-0485 - p. 6046 - 6053.
    Microalgae - Mild cell disruption - Multiproduct biorefinery - Selective release

    Pulsed electric field (PEF) is considered to be a very promising technology for mild cell disruption. The application of PEF for microalgae that have a rigid cell wall, however, is hampered by the presence of that rigid outer cell wall. A cell wall free mutant of C. reinhardtii was used to mimic pretreated microalgae with removed cell wall, to investigate the possibility of using PEF for protein release from microalgae. A complete release of hydrophilic proteins from the cell wall free mutants was observed whereas PEF treatment on the cell wall containing species resulted in substantially lower protein yields. Additional experiments showed that even at low energy input (0.05 kWh/kgbiomass), still about 70% of the proteins could be released with respect to bead beating as reference. These released proteins were water-soluble while the hydrophobic chlorophyll remained mainly entrapped in cell particles. SEM-analysis of these cell particles showed that PEF only opened the cells, instead of completely fragmenting them into smaller particles. These results indicate that PEF is an energy-efficient cell disruption method for selective release of water-soluble proteins, after the microalgal outer cell wall is removed. Enzymatic pretreatment to degrade the cell walls before PEF treatment was shown to be an efficient method to remove the cell wall.

    Adipose tissue meal-derived fatty acid uptake before and after diet-induced weight loss in adults with overweight and obesity
    Vink, Roel G. ; Roumans, Nadia J.T. ; Kolk, Birgitta W. van der; Fazelzadeh, Parastoo ; Boekschoten, Mark V. ; Mariman, Edwin C.M. ; Baak, Marleen A. van - \ 2017
    Obesity 25 (2017)8. - ISSN 1930-7381 - p. 1391 - 1399.
    Objective: This study investigated whether diet-induced weight loss alters indices of in vivo postprandial fat uptake in adipose tissue (AT) and whether these changes are associated with weight regain in adults with overweight and obesity. Methods: In this randomized controlled trial, 16 (6 male) individuals (BMI: 28-35 kg/m2) were randomized to either a low-calorie diet (1,250 kcal/d) for 12 weeks or a very-low-calorie diet (500 kcal/d) for 5 weeks (weight loss [WL] period) followed by a 4-week weight-stable (WS) period (together, the dietary intervention [DI] period) and a 9-month follow-up period. Arteriovenous difference measurements combined with stable isotope labeling ([U-13C] palmitate) of a mixed meal were used to determine postprandial fatty acid uptake in AT. Results: Body weight was significantly reduced during the WL period (-8.2±0.6 kg, P<0.001), remained stable during the WS period (0.4±0.3 kg, P=0.150), and increased during follow-up (3.5±0.8 kg, P=0.001). Meal-derived in vivo fatty acid uptake dynamics across AT and expression of genes important for fatty acid uptake, storage, and release were not significantly changed during the DI period. Conclusions: Subcutaneous AT does not appear prone to enhanced meal-derived fatty acid uptake after weight loss, nor were fatty acid uptake dynamics detected as related to weight regain.
    Greenhouse gas reporting for the LULUCF sector in the Netherlands : methodological background, update 2016
    Arets, E.J.M.M. ; Kolk, J.W.H. van der; Hengeveld, G.M. ; Lesschen, J.P. ; Kramer, H. ; Kuikman, P.J. ; Schelhaas, M.J. - \ 2017
    Wageningen : Statutory Research Tasks Unit for Nature & the Environment (WOt-technical report 89) - 90
    greenhouse gases - methodology - emission - netherlands - broeikasgassen - methodologie - emissie - nederland
    This report provides a complete methodological description and background information of the DutchNational System for Greenhouse gas Reporting of the LULUCF sector. It provides detailed description of themethodologies, activity data and emission factors that were used. Additionally it gives a table-by-tableelaboration of the choices and motivations for filling the CRF tables for KP-LULUCF
    Samenstelling van blad, stengel en rhizomen in relatie tot optimaal oogst-tijdstip van Miscanthus x giganteus
    Kasper, G.J. ; Kolk, J.C. van der; Putten, J.C. van der - \ 2017
    Wageningen : Wageningen Livestock Research (Wageningen Livestock Research rapport 1022) - 27
    brandstofgewassen - biobased economy - miscanthus - grassen - oogsttijdstip - gewasopbrengst - akkerbouw - plantensamenstelling - suikergehalte - lignine - pectinen - droge stof - koolhydraten - stengels - wortelstokken - fuel crops - biobased economy - miscanthus - grasses - harvesting date - crop yield - arable farming - plant composition - sugar content - lignin - pectins - dry matter - carbohydrates - stems - rhizomes
    A plurality of components (such as sugars, lignin, pectin) of Miscanthus x giganteus has been studied in stem, leaf, and rhizomes for the harvest times July and January in view of the optimal harvest time. Additional literature search shows that the end of October is the optimum time for harvesting on the basis of the maximum above-ground dry matter yield and sugar yield, and dry matter yield in the next year. It will have to be investigated whether the optimal harvest time also applies to long-term research.
    Potential Arctic tundra vegetation shifts in response to changing temperature, precipitation and permafrost thaw
    Kolk, Henk-Jan van der; Heijmans, M.M.P.D. ; Huissteden, J. van; Pullens, J.W.M. ; Berendse, F. - \ 2016
    Biogeosciences 13 (2016)22. - ISSN 1726-4170 - p. 6229 - 6245.
    Over the past decades, vegetation and climate have changed significantly in the Arctic. Deciduous shrub cover is often assumed to expand in tundra landscapes, but more frequent abrupt permafrost thaw resulting in formation of thaw ponds could lead to vegetation shifts towards graminoid-dominated wetland. Which factors drive vegetation changes in the tundra ecosystem are still not sufficiently clear. In this study, the dynamic tundra vegetation model, NUCOM-tundra (NUtrient and COMpetition), was used to evaluate the consequences of climate change scenarios of warming and increasing precipitation for future tundra vegetation change. The model includes three plant functional types (moss, graminoids and shrubs), carbon and nitrogen cycling, water and permafrost dynamics and a simple thaw pond module. Climate scenario simulations were performed for 16 combinations of temperature and precipitation increases in five vegetation types representing a gradient from dry shrub-dominated to moist mixed and wet graminoid-dominated sites. Vegetation composition dynamics in currently mixed vegetation sites were dependent on both temperature and precipitation changes, with warming favouring shrub dominance and increased precipitation favouring graminoid abundance. Climate change simulations based on greenhouse gas emission scenarios in which temperature and precipitation increases were combined showed increases in biomass of both graminoids and shrubs, with graminoids increasing in abundance. The simulations suggest that shrub growth can be limited by very wet soil conditions and low nutrient supply, whereas graminoids have the advantage of being able to grow in a wide range of soil moisture conditions and have access to nutrients in deeper soil layers. Abrupt permafrost thaw initiating thaw pond formation led to complete domination of graminoids. However, due to increased drainage, shrubs could profit from such changes in adjacent areas. Both climate and thaw pond formation simulations suggest that a wetter tundra can be responsible for local shrub decline instead of shrub expansion.
    Referentieraming van emissies naar lucht uit de landbouw tot 2030 : achtergronddocument bij de Nationale Energieverkenning 2015, met emissies van ammoniak, methaan, lachgas, stikstofoxide en fijnstof uit de landbouw tot 2030
    Velthof, G.L. ; Bruggen, C. van; Groenestein, C.M. ; Huijsmans, J.F.M. ; Luesink, H.H. ; Sluis, S.M. van der; Kolk, J.W.H. van der; Oude Voshaar, S.V. ; Vonk, J. ; Schijndel, M.W. van - \ 2016
    Wageningen : Wageningen Environmental Research (Wageningen Environmental Research rapport 2746) - 75
    luchtverontreiniging - ammoniakemissie - methaan - distikstofmonoxide - stikstofoxide - fijn stof - landbouw - nederland - air pollution - ammonia emission - methane - nitrous oxide - nitric oxide - particulate matter - agriculture - netherlands
    Angiopoietin-Like Protein 4 and Postprandial Skeletal Muscle Lipid Metabolism in Overweight and Obese Prediabetics
    Kolk, Birgitta W. van der; Goossens, Gijs H. ; Jocken, Johan W. ; Kersten, Sander ; Blaak, Ellen E. - \ 2016
    Journal of Clinical Endocrinology and Metabolism 101 (2016)6. - ISSN 0021-972X - p. 2332 - 2339.
    Context: Angiopoietin-like protein 4 (ANGPTL4) decreases plasma triacylglycerol (TAG) clearance by inhibiting lipoprotein lipase (LPL) and may contribute to impairments in lipid metabolism under compromised metabolic conditions. Objectives: To investigate the effects of a high-saturated fatty acid (SFA) mixed meal on plasma ANGPTL4 concentrations in relation to in vivo muscle LPL activity, to study the effects of dietary fat quality, and to examine skeletal muscle ANGPTL4 release. Design, Participants, Setting, and Interventions: We used a dual stable-isotope tracer technique in combination with measurements of arteriovenous concentration differences across forearm muscle to investigate muscle ANGPTL4 secretion and fatty acid handling under fasting conditions and after a high-SFA mixed meal in 73 overweight and obese humans at the Metabolic Research Unit of Maastricht University. The effect of dietary fat quality manipulation on plasma ANGPTL4 was investigated in 10 obese insulin-resistant participants. Results: The high-SFA meal decreased circulating ANGPTL4 concentrations (fasting, 5.2 ng/mL; vs 4 hours postprandial, 4.0 ng/mL; P < .001). Furthermore, skeletal muscle ANGPTL4 secretion into the circulation was observed (AUC0–4 h, P = .048). However, no association was observed between plasma ANGPTL4 and skeletal muscle very low-density lipoprotein or dietary (chylomicron) TAG extraction (AUC0–4 h, P = .372 and P = .139, respectively). In contrast to a high-SFA or high-monounsaturated fat meal, plasma ANGPTL4 remained unchanged after a high-polyunsaturated fat meal. Conclusions: ANGPTL4 is secreted by human forearm muscle in postprandial conditions after a high-SFA meal. Plasma ANGPTL4 concentrations were not associated with in vivo skeletal muscle LPL activity after a high-SFA meal. Dietary fat quality affects plasma ANGPTL4, but it remains to be elucidated whether this influences short-term skeletal muscle lipid handling. “This human in vivo study showed ANGPTL4 secretion from forearm muscle after a high-fat meal. Plasma ANGPTL4 was not associated with muscle LPL activity, but was affected by dietary fat quality
    Methodology for estimating emissions from agriculture in the Netherlands. : Calculations of CH4, NH3, N2O, NOx, PM10, PM2.5 and CO2 with the National Emission Model for Agriculture (NEMA)
    Vonk, J. ; Bannink, A. ; Bruggen, C. van; Groenestein, C.M. ; Huijsmans, J.F.M. ; Kolk, J.W.H. van der; Luesink, H.H. ; Oude Voshaar, S.V. ; Sluis, S.M. ; Velthof, G.L. - \ 2016
    Wageningen : Statutory Research Tasks Unit for Nature & the Environment (WOt-technical report 53) - 164
    air pollutants, greenhouse gases, livestock, crops, animal housing, manure storage, manure application, inorganic fertilizer, enteric fermentation, manure management, agricultural soils, liming, NIR, CRF, IIR, NFR - landbouw - gewassen - landbouwgronden - vee - huisvesting, dieren - dierlijke meststoffen - rundveemest - mestverwerking - begrazing - broeikasgassen - luchtverontreinigende stoffen - emissie - ammoniakemissie - kooldioxide - methaan - anorganische meststoffen - fermentatie - bekalking - nederland - compost - rioolslib - teelt - oogstresten - rijp worden - agriculture - crops - agricultural soils - livestock - animal housing - animal manures - cattle manure - manure treatment - grazing - greenhouse gases - air pollutants - emission - ammonia emission - carbon dioxide - methane - inorganic fertilizers - fermentation - liming - netherlands - composts - sewage sludge - cultivation - crop residues - ripening
    The National Emission Model for Agriculture (NEMA) is used to calculate emissions to air from agricultural activities in the Netherlands on a national scale. Emissions of ammonia (NH3) and other N-compounds (NOx and N2O) from animal housing, manure storage, manure application and grazing are assessed using a Total Ammoniacal Nitrogen (TAN) flow model. Furthermore, emissions from application of inorganic N-fertilizer, compost and sewage sludge, cultivation of organic soils, crop residues, and ripening of crops are calculated. NEMA is also used to estimate emissions of methane (CH4) from enteric fermentation and manure management, particulate matter (PM) from manure management and agricultural soils, and carbon dioxide
    (CO2) from liming. Emissions are calculated in accordance with international guidance criteria and reported in an annual Informative Inventory Report (IIR; for air pollutants) and National Inventory Report (NIR; for greenhouse gases). This methodology report describes the outline and backgrounds of the emission
    calculations with NEMA
    Ontwikkeling van toepassingen met plasmawater in de glastuinbouw
    Quaedvlieg, William ; Hofland-Zijlstra, J.D. ; Hollinger, T.C. ; Noordam, Marianne ; Slootweg, G. ; Broek, R.C.F.M. van den; Kolk, Jan Paul van der; Ruijven, J.P.M. van; Stijger, I. - \ 2016
    Bleiswijk : Wageningen UR Glastuinbouw (Rapport GTB 1391) - 68
    teelt onder bescherming - gewasbescherming - biociden - desinfectie - irrigatiewater - radicalen - bevruchtingsvermogen - protected cultivation - plant protection - biocides - disinfection - irrigation water - radicals - fertilizing ability
    In collaboration with Vitalfluid, TU Eindhoven, FloraHolland, Bactimm, Alewijnse and Medical faculty of UMC Radboud Wageningen UR Greenhouse Horticulture studied horticultural application for the use of plasma activated water. This research was funded by the GO programm Oost Nederland, the European Fund for Regional Development (EFRO) and Top sector Horticulture & Propagation materials. By using a specialized plasma reactor, an indirect air dielectric barrier discharge in close proximity to water can create an acidified, nitrogen-oxide containing solution called plasma-activated water (PAW). Plasma water is tested for properties as a biocidal product, plant protection treatment and as a fertilizer in horticultural applications. Mild plasma water with a short production time (15 min) can effectively control bacteria and meet the biocidal product legislation. Strong plasma water with a longer production time (45 min) is necessary for controlling molds as Fusarium, Botrytis and powdery mildew. The tomato mosaic virus can be reduced with strong plasma up to 80%. The crop treatments showed no damage on young gerbera plants, lettuce and tomato plants. Application of plasma water through the irrigation system to promote plant growth is not promising, because nitrogen supply is in general not limited. Application as seed disinfection is possible with low concentrations of plasma water.
    Using a phenological network to assess weather influences on first appearance of butterflies in the Netherlands
    Kolk, Henk Jan Van Der; Wallis de Vries, Michiel ; Vliet, Arnold J.H. Van - \ 2016
    Ecological Indicators 69 (2016). - ISSN 1470-160X - p. 205 - 212.
    Anthocharis cardamines - Butterflies - Climate change - Monitoring network - Phenology - Plant-host interaction

    Phenological responses of butterflies to temperature have been demonstrated in several European countries by using data from standardized butterfly monitoring schemes. Recently, phenological networks have enabled volunteers to record phenological observations at project websites. In this study, the quality of the first appearance data of butterflies from the Dutch phenological network 'De Natuurkalender' was examined and these data were then used to analyze trends in butterfly appearance between 2001 and 2013, the effects of climatic factors on appearance of butterflies as well as the phenological interaction of one butterfly species, Anthocharis cardamines, and its two major host plants. Although phenological networks are relatively unstructured, this study shows that data from De Natuurkalender were highly comparable to more standardized data collected by the Dutch Butterfly Monitoring Scheme. No trend in first appearance of any butterfly species was found during the time period 2001-2013. The first appearance dates of most butterflies showed, however, a clear relationship with spring temperature. Higher temperatures, especially in March and April, advanced the first appearance of butterflies. Therefore, with climatic warming in the future, earlier appearance of butterflies is expected. Although climate warming is a potential threat for phenological mismatches between different trophic levels, this study shows a similar temperature response of A. cardamines and its main host plants in the Netherlands. However, as only few phenological interactions between species are examined, further research including rarer monophagous butterfly species and their host plants is needed.

    Ziekteweerbaarheid verhogen tegen wortelknobbelaaltjes (Meloidogyne spp.)
    Kolk, J.P. van der; Voogt, W. ; Streminska, M.A. ; Wurff, A.W.G. van der - \ 2016
    - 1
    tuinbouw - gewasbescherming - plagenbestrijding - meloidogyne - glastuinbouw - biologische bestrijding - compost - bodemvruchtbaarheid - tomaten - conferenties - biologische landbouw - plaagbestrijding met predatoren - vruchtgroenten - meloidogyne incognita - meloidogyne javanica - aaltjesdodende eigenschappen - plantenparasitaire nematoden - bodemweerbaarheid - horticulture - plant protection - pest control - meloidogyne - greenhouse horticulture - biological control - composts - soil fertility - tomatoes - conferences - organic farming - predator augmentation - fruit vegetables - meloidogyne incognita - meloidogyne javanica - nematicidal properties - plant parasitic nematodes - soil suppressiveness
    In de biologische glastuinbouw zijn bodem gebonden ziektes een groot knelpunt. Wortelknobbelaaltjes (Meloidogyne incognita en M. javanica) zijn belangrijke pathogenen die in de vruchtgroententeelt voor problemen zorgen.
    Plasmawater, een veelbelovend ontsmettings- en gewasbeschermingsmiddel
    Quaedvlieg, William ; Hofland-Zijlstra, J.D. ; Lemmers, M.E.C. ; Ruijven, J.P.M. van; Kolk, J.P. van der; Slootweg, G. ; Stijger, I. - \ 2016
    Wageningen : Wageningen UR Glastuinbouw - 1
    tuinbouw - gewasbescherming - pectobacterium carotovorum - botrytis cinerea - fusarium - tomatenmozaïekvirus - gerbera - biociden - landbouwkundig onderzoek - fytotoxiciteit - corrosie - ontsmettingsmiddelen - glastuinbouw - horticulture - plant protection - pectobacterium carotovorum - botrytis cinerea - fusarium - tomato mosaic virus - gerbera - biocides - agricultural research - phytotoxicity - corrosion - disinfectants - greenhouse horticulture
    Voor dit onderzoek is gekeken of plasmawater onder laboratorium- en semi-praktijk condities verschillende soorten micro-organismen (bacteriën, schimmels en virussen) kan afdoden op het gewas. Tevens is gelet op de veiligheid voor het gewas (fyto-tox) en kas (corrosie). Poster van het PlantgezondheidEvent 2016.
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