Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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    Human buccal epithelium acquires microbial hyporesponsiveness at birth, a role for secretory leukocyte protease inhibitor
    Menckeberg, C.L. ; Hol, J. ; Simons-Oosterhuis, Y. ; Raatgeep, H.R. ; Ruiter, L.F. de; Lindenbergh-Kortleve, D.J. ; Korteland-van Male, A.M. ; Aidy, S.F. El; Lierop, P.P.E. van; Kleerebezem, M. ; Groeneweg, M. ; Kraal, G. ; Elink-Schuurman, B.E. ; Jongste, J.C. de; Nieuwenhuis, E.E.S. ; Samsom, J.N. - \ 2015
    Gut 64 (2015). - ISSN 0017-5749 - p. 884 - 893.
    toll-like receptors - negative regulation - intestinal-mucosa - crohn-disease - responses - cells - tolerance - lipopolysaccharide - inflammation - mechanisms
    Objective Repetitive interaction with microbial stimuli renders epithelial cells (ECs) hyporesponsive to microbial stimulation. Previously, we have reported that buccal ECs from a subset of paediatric patients with Crohn's disease are not hyporesponsive and spontaneously released chemokines. We now aimed to identify kinetics and mechanisms of acquisition of hyporesponsiveness to microbial stimulation using primary human buccal epithelium. Design Buccal ECs collected directly after birth and in later stages of life were investigated. Chemokine release and regulatory signalling pathways were studied using primary buccal ECs and the buccal EC line TR146. Findings were extended to the intestinal mucosa using murine model systems. Results Directly after birth, primary human buccal ECs spontaneously produced the chemokine CXCL-8 and were responsive to microbial stimuli. Within the first weeks of life, these ECs attained hyporesponsiveness, associated with inactivation of the NF-¿B pathway and upregulation of the novel NF-¿B inhibitor SLPI but no other known NF-¿B inhibitors. SLPI protein was abundant in the cytoplasm and the nucleus of hyporesponsive buccal ECs. Knock-down of SLPI in TR146-buccal ECs induced loss of hyporesponsiveness with increased NF-¿B activation and subsequent chemokine release. This regulatory mechanism extended to the intestine, as colonisation of germfree mice elicited SLPI expression in small intestine and colon. Moreover, SLPI-deficient mice had increased chemokine expression in small intestinal and colonic ECs. Conclusions We identify SLPI as a new player in acquisition of microbial hyporesponsiveness by buccal and intestinal epithelium in the first weeks after microbial colonisation.
    Temporal and spatial interplay of microbiota and intestinal mucosa drive establishment of immune homeostasis in conventionalized mice
    Aidy, S. El; Baarlen, P. van; Derrien, M. ; Lindenbergh-Kortleve, D.J. ; Hooiveld, G.J. ; Levenez, F. ; Dore, J. ; Dekker, J. ; Samsom, J.N. ; Nieuwenhuis, E.E.S. ; Kleerebezem, M. - \ 2012
    Mucosal Immunology 5 (2012). - ISSN 1933-0219 - p. 567 - 579.
    inflammatory-bowel-disease - t-cell development - expression - responses - mechanisms - receptors - induction - bacteria - defense - mucins
    During colonization of germfree mice with the total fecal microbial community of their conventionally born and raised siblings (conventionalization), the intestinal mucosal immune system initiates and maintains a balanced immune response. However, the genetic regulation of these balanced, appropriate responses to the microbiota is obscure. Here, combined analysis of germfree and conventionalized mice revealed that the major molecular responses could be detected initiating at day 4 post conventionalization, with a strong induction of innate immune functions followed by stimulation of adaptive immune responses and development and expansion of adaptive immune cells at later stages of conventionalization. This study provides a comprehensive overview of mouse developmental and immune-related cellular pathways and processes that were co-mediated by the commensal microbiota and suggests which mechanisms were involved in this reprogramming. The dynamic, region-dependent mucosal responses to the colonizing microbiota revealed potential transcriptional signatures for the control of intestinal homeostasis in healthy mice, which may help to decipher the genetic basis of pathway dysregulation in human intestinal inflammatory diseases
    The influence of maltodextrins on the structure and properties of compression-molded starch plastic sheets
    Soest, J.J.G. van; Kortleve, P.M. - \ 1999
    Journal of Applied Polymer Science 74 (1999)9. - ISSN 0021-8995 - p. 2207 - 2219.
    Starch plastic sheets were prepared by compression molding of starch-based plastic granulates. The granulates were prepared by extrusion processing of mixtures of granular potato starch and several maltodextrins (5% w/w) in the presence of glycerol and water as plasticizers and lecithin as melt flow accelerator. The materials were semicrystalline, containing B-type, Vh-type, and Eh-type crystallinity. The properties were dependent on water content. For the materials, a brittle-to-ductile transition occurred at a water content in the range of 11-12%, which was in accordance with the observed glass transition temperature. The structural and mechanical properties were a function of starch composition and maltodextrin source as well as molding temperature. The amount of granular remnants and residual B-type crystallinity decreased with increasing processing temperature. The amount of recrystallized single-helical amylose increased with increasing temperature. At molding temperatures in the range of 180-200°C, a sharp decrease in starch molecular mass occurred. The influence of molding temperature was reflected in a sharp increase in elongation at molding temperature above 160°C and a gradual decrease in elastic modulus. The tensile strength showed an initial small increase up to 160°C and a sharp decrease at higher molding temperatures.
    Mechanisatie en opslag bij de teelt van Miscanthus.
    Huisman, W. ; Kortleve, W.J. - \ 1995
    In: Verslag 5e Nederlandse Zonne-energie Conferentie, Veldhoven, The Netherlands - p. 66 - 69.
    Plantmethoden voor Miscanthus giganteus.
    Kortleve, W.J. ; Huisman, W. - \ 1995
    Landbouwmechanisatie 46 (1995)2. - ISSN 0023-7795 - p. 14 - 15.
    bio-energie - biomassa - celkweek - kostenanalyse - droge stof - energie - vezelgewassen - meristemen - beplanten - wortelstokken - weefselkweek - knollen - nuttig gebruik - bioenergy - biomass - cell culture - cost analysis - dry matter - energy - fibre plants - meristems - planting - rhizomes - tissue culture - tubers - utilization
    Teeltaanwijzingen met een kostenoverzicht voor de aanplant van een hectare nieuw gewas door middel van meristeemplanten en door middel van rhizomen
    Opslag en bewaring van Miscanthus giganteus.
    Kortleve, W.J. ; Huisman, W. - \ 1995
    Landbouwmechanisatie 46 (1995)8. - ISSN 0023-7795 - p. 20 - 21.
    bio-energie - biomassa - hakselen - schoonmaken - energie - indeling - grassen - afpellen - planten - poaceae - wassen (activiteit) - nuttig gebruik - voedergrassen - bioenergy - biomass - chopping - cleaning - energy - grading - grasses - peeling - plants - poaceae - washing - utilization - fodder grasses
    Mechanisatie Miscanthus.
    Kortleve, W.J. ; Huisman, W. - \ 1994
    Unknown Publisher - 21 p.
    Mechanisation of harvest and conservation of Miscanthus sinensis giganteus.
    Huisman, W. ; Kortleve, W.J. - \ 1994
    In: Workshop programme Abstracts; COST 814: Alternative oilseed and fibre crops for cool and wet regions of Europe. Wageningen - p. 23 - 23.
    Mechanisation of crop establishment, harvest and post harvest conservation of Miscanthus sinensis Giganteus.
    Huisman, W. ; Kortleve, W.J. - \ 1994
    Industrial Crops and Products 2 (1994). - ISSN 0926-6690 - p. 289 - 297.
    Mogelijkheden en methoden voor Miscanthus (olifantsgras).
    Huisman, W. ; Kortleve, W.J. - \ 1994
    In: Techniek, energie en milieu; Verslag Studiedag Ned. Ver. Techniek in de Landbouw en Vlaams Genootschap voor Landbouwtechniek, Vinkeloord. Wageningen Pers - p. 40 - 42.
    Mechanisation of harvest and conservation of Miscanthus sinensis giganteus.
    Huisman, W. ; Kortleve, W.J. - \ 1994
    In: Alternative oilseed and fibre crops for cool and wet regions of Europe, COST 814 Workshop Proc. Wageningen - p. 91 - 100.
    Verslag van een studiereis naar Engeland van 15 tot 21 juli 1973
    Kortleve, C. ; Oosten, H.J. van; Uitterlinden, L. - \ 1973
    Wilhelminadorp : Proefstation voor de Fruitteelt - 23
    vruchtbomen - groot-brittannië - boomgaarden - fruit trees - great britain - orchards
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